In-silico formulation of a next-generation polyvalent vaccine against multiple strains of monkeypox virus and other related poxviruses. by Abu Tayab Moin, Nurul Amin Rani, Rajesh B Patil, Tanjin Barketullah Robin, Md Asad Ullah, Zahidur Rahim, Md Foyzur Rahman, Talha Zubair, Mohabbat Hossain, A K M Moniruzzaman Mollah, Nurul Absar, Mahboob Hossain, Mohammed Abul Manchur, Nazneen Naher Islam

“…This study utilized a reverse vaccinology approach to retrieve conserved epitopes for monkeypox virus and construct a vaccine that could provide cross-protection against related viruses with similar antigenic properties. The selected virulent proteins of monkeypox virus, MPXVgp165, and Virion core protein P4a, were subjected to epitope mapping for vaccine construction. …”
Get full text

Homeopathic Treatment of Arabidopsis thaliana Plants Infected with Pseudomonas syringae by Devika Shah-Rossi, Peter Heusser, Stephan Baumgartner

“…We investigated the potential of a common plant-pathogen system, Arabidopsis thaliana infected with the virulent bacteria Pseudomonas syringae, regarding its response towards a homeopathic treatment. …”
Get full text

CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots by Maria A. Lebedeva, Maria S. Gancheva, Maksim R. Losev, Sofia V. Sokornova, Oleg S. Yuzikhin, Anna A. Krutikova, Kirill V. Plemyashov, Lyudmila A. Lutova

“…However, the selection of new resistant sunflower varieties was accompanied by the emergence of new virulent races of broomrape, which overcame the effect of resistance genes. …”
Get full text

Potato resistance to quarantine diseases by A. V. Khiutti, O. Yu. Antonova, N. V. Mironenko, T. A. Gavrilenko, O. S. Afanasenko

“…Cultivation of cultivars poorly susceptible to PGN can stimulate the adaptive variability of the pathogen and induce virulent pathotypes for 2–3 pathogen generations.…”
Get full text

Low-Level Zoonotic Transmission of Clade C MERS-CoV in Africa: Insights from Scoping Review and Cohort Studies in Hospital and Community Settings by Andrew Karani, Cynthia Ombok, Silvia Situma, Robert Breiman, Marianne Mureithi, Walter Jaoko, M. Kariuki Njenga, Isaac Ngere

“…Ongoing research focuses on genomic comparisons between clade C and the more virulent clades A and B, alongside the surveillance of viral evolution. …”
Get full text

The Generation and Characterization of Recombinant Protein and Antibodies of Clostridium perfringens Beta2 Toxin by Jin Zeng, Fuyang Song, Yi Yang, Chenjie Ma, Guangcun Deng, Yong Li, Yujiong Wang, Xiaoming Liu

“…Clostridium perfringens (C. perfringens) beta2 toxin (CPB2) is an important virulent factor of necrotic enteritis in both animals and humans. …”
Get full text

Mendelian Susceptibility to Mycobacterial Diseases (MSMD) in a 13-Year-Old Ethiopian Girl with Autosomal Dominant Interferon Gamma Receptor 1(IFN-γ R1) Defect: A Clinical Diagnosti... by Netsanet Azene Gebeyehu, Solomie Jebessa Deribessa, Freeman Alexandra, Messay Tesfaye Demissie, W Mihretu Gebre, Aklilu Melaku Gebremariam, Dagmawit Mitiku Engliz, Tizita Yosef Kidane, Lidya Million Bekele, Abate Yeshidinber Weldetsadik

“…MSMD is characterized by vulnerability to less virulent mycobacteria, such as Bacillus Calmette-Guérin (BCG) vaccine strains, as well as environmental mycobacteria (EM). …”
Get full text

NERVE 2.0: boosting the new enhanced reverse vaccinology environment via artificial intelligence and a user-friendly web interface by Andrea Conte, Nicola Gulmini, Francesco Costa, Matteo Cartura, Felix Bröhl, Francesco Patanè, Francesco Filippini

“…Along with two newly built AI (Artificial Intelligence)-based models: ESPAAN and Virulent. To improve user-friendliness, NERVE was shifted to a tutored, web-based interface, with a noSQL-database to consent the user to submit, obtain and retrieve analysis results at any moment. …”
Get full text

ANTIGENIC AND PROTECTIVE PROPERTIES OF ASSOCIATED VIRUS VACCINE AGAINST SHEEP POX, GOAT POX AND PESTE DES PETITS RUMINANTS by A. V. Konstantinov, S. K. Starov, V. I. Diev, N. V. Moroz, E. V. Kurnenkova, D. K. Basova, A. V. Kononov, K. Yu. Fedoseyev, V. P. Melnikov

“…The vaccination induced high level of virus neutralizing antibodies against each of the above listed diseases and ensured formation of strong immunity tat is supported by the results of tallenge of sheep with virulent sheep pox virus (“Afghanistan” strain). In 14 and 21 days in blood of sheep immunized with the virus vaccine against sheep pox, goat pox and peste des petits rum^nts titres of antibodies against sheep pox virus ((4.17±0.12)-(4.84±0.31)) and PPR ((5.92±0.23)-(6.08±0.23)) were higher as compared to goat pox antibody titres ((1.99±0.89)-(3.53±0.58) log2). …”
Get full text

ANTIGENIC AND PROTECTIVE PROPERTIES OF EXPERIMENTAL ASSOCIATED VIRUS VACCINE AGAINST SHEEP POX AND GOAT POX by A. V. Konstantinov, S. K. Starov, V. I. Diev, N. V. Moroz, E. V. Kurnenkova, D. К. Basova, А. V. Kononov, K. Yu. Fedoseyev

“…All sheep immunized with the monovalent vaccine against sheep and goat pox and associated virus vaccines, resisted the disease when challenged with a virulent sheep pox virus “Afghanistan” strain. Three sheep vaccinated with a monovalent vaccine against goat pox demonstrated a large local reaction, one sheep was diagnosed with generalized form of sheep pox.…”
Get full text

Toxin and capsule production by Bacillus cereus biovar anthracis influence pathogenicity in macrophages and animal models. by Treenate Jiranantasak, Andrew P Bluhm, Donald J Chabot, Arthur Friedlander, Richard Bowen, Ian A McMillan, Ted L Hadfield, Airn Hartwig, Jason K Blackburn, Michael H Norris

“…Our research demonstrated that Bcbva is more virulent than B. anthracis Ames using two in vivo models, Galleria mellonella larvae and guinea pigs. …”
Get full text

Field apple scab susceptibility of a diverse Malus germplasm collection identifies potential sources of resistance for apple breeding by David Papp, Liqiang Gao, Ranjita Thapa, Dan Olmstead, Awais Khan

“…We also screened a differential host set for 2 years to monitor for changes in the effectiveness of ten known scab resistance genes, which allowed us to confirm the presence of virulent pathogen races in the orchard. Results We found that ~ 37% of the wild Malus accessions and domesticated cultivars were resistant to apple scab in the field. …”
Get full text

Properties of Actinobacillus pleuropneumoniae isolates by V. А. Evgrafova, О. V. Pruntova, N. B. Shadrova, А. М. Timina

“…Isolate No. 4 belonging to serotype 5 was found to be the most virulent out of tested isolates. Thus, LD50 was 4.19 lg microbial cells for white mice and 5.49 lg microbial cells for piglets that was consistent to the data of other authors carried out tests of actinobaccilli isolated in the Russian Federation for their pathogenicity. …”
Get full text

Identification of site-specific adaptations conferring increased neural cell tropism during human enterovirus 71 infection. by Samuel Cordey, Tom J Petty, Manuel Schibler, Yannick Martinez, Daniel Gerlach, Sandra van Belle, Lara Turin, Evgeny Zdobnov, Laurent Kaiser, Caroline Tapparel

“…Enterovirus 71 (EV71) is one of the most virulent enteroviruses, but the specific molecular features that enhance its ability to disseminate in humans remain unknown. …”
Get full text

Results of scientific expedition to natural biotopes of the Republic of Tyva in 2019 with the purpose of infectious disease monitoring in wild bird populations by M. S. Volkov, V. N. Irza, A. V. Varkentin, S. V. Rogolyov, A. V. Andriyasov

“…Notwithstanding the absence of AIV very virulent isolate detections in wild bird populations the middle term prognosis for 2020 can be designated as cautious, as the avian influenza epidemic situation is deteriorating globally, especially in the European countries, and the threat of the virus introduction to the Russian territory with migratory birds still exists.…”
Get full text

Prophage-DB: a comprehensive database to explore diversity, distribution, and ecology of prophages by Etan Dieppa-Colón, Cody Martin, James C. Kosmopoulos, Karthik Anantharaman

“…Phages are classified as virulent or temperate based on their life cycles. Temperate phages adopt the lysogenic mode of infection, where the genome integrates into the host cell genome forming a prophage. …”
Get full text

Use of “ARRIAH-AviFluVac” vaccine in turkeys, geese and ducks by N. V. Moroz, S. V. Frolov, V. N. Irza, L. O. Scherbakova, V. Yu. Kulakov

“…The vaccine facilitated 9–26-fold decrease in the virulent virus shedding by the ducks. Protection of turkeys vaccinated at the dose of 1.0 cm3 was maintained at the level of 87.5% after challenge with high pathogenicity avian influenza virus of subtype H5N1, clade 2.3.4.4b. …”
Get full text

Marek's disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius. by Chenyi Bao, Jun Chu, Qi Gao, Shasha Yang, Xiaoyu Gao, Wenwen Chen, Fuchun Yang, Fei Jiang, Chenxi Tong, Mingyi Lei, Linlin Jiao, Jitong Li, Kexin Wei, Xue Lian, Kai Li, Suresh Kumar Tikoo, Nikolaus Osterrieder, Lorne A Babiuk, Yufeng Li, Yong-Sam Jung, Yingjuan Qian

“…To this end, an infectious bacterial artificial chromosome (BAC) harboring the complete genome of the MDV-1 very virulent strain Md5 was generated, and the rescued rMd5 maintained biological properties similar to the parental virus both in vitro and in vivo. …”
Get full text

High content analysis of primary macrophages hosting proliferating Leishmania amastigotes: application to anti-leishmanial drug discovery. by Nathalie Aulner, Anne Danckaert, Eline Rouault-Hardoin, Julie Desrivot, Olivier Helynck, Pierre-Henri Commere, Hélène Munier-Lehmann, Gerald F Späth, Spencer L Shorte, Geneviève Milon, Eric Prina

“…These data thus validate a novel phenotypic screening assay using virulent Leishmania amastigotes growing inside primary macrophage to identify new chemical entities with bona fide drug potential.…”
Get full text

Transformational technologies for more uptakes of entomopathogenic nematodes by Mahfouz M. M. Abd-Elgawad

“…Classical breeding of EPNs could obtain Heterorhabditis bacteriophora-infective juveniles with so virulent and persistent superiority that application rates and consequent costs were reduced into the range of chemical insecticides. …”
Get full text