Dissecting the biophysical mechanisms of oleate hydratase association with membranes by William A. Lathram, Robert J. Neff, Ashley N. Zalla, James D. Brien, Vivekanandan Subramanian, Christopher D. Radka

“…These findings advance our understanding of the biophysical principles underlying OhyA’s role in bacterial membrane function and virulence.…”
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Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology by Qi Wu, Chen Sun, Liping Guo, Yujia Xie, Jinpeng Zhang, Jinpeng Zhang, Dehui Yin, Dehui Yin

“…VirB proteins, components of the Type IV secretion system (T4SS) in Brucella, play a pivotal role in bacterial virulence and pathogenesis but have been understudied for their diagnostic potential.MethodsTandem Mass Tag (TMT) proteomics technology was utilized to identify highly expressed VirB proteins from wild-type Brucella strains. …”
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Understanding the Impact of Salt Stress on Plant Pathogens Through Phenotypic and Transcriptomic Analysis by Hyejung Jung, Gil Han, Duyoung Lee, Hyun-Kyoung Jung, Young-Sam Kim, Hee Jeong Kong, Young-Ok Kim, Young-Su Seo, Jungwook Park

“…For plant diseases to become established, plant pathogens require not only virulence factors and susceptible hosts, but also optimal environmental conditions. …”
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Streptococcus suis-associated neonatal meningitis and sepsis: characterization, antimicrobial resistance, and public health implications by Giovanna Fusco, Rubina Paradiso, Lorena Cardillo, Maria Antonia Salvia, Saveria Dodaro, Veronica Del Monaco, Gianfranco Scarpelli, Francesca Greco, Antonio Rinaldi, Lorella Barca, Stefania Ambrogio, Antonio Limone, Esterina De Carlo, Giorgia Borriello

“…Next-generation sequencing revealed that the strain was serotype 2 sequence type 1, and contained the ermB and tet(W) genes, which are responsible for resistance to macrolides and tetracycline, along with several pilus-associated genes and 20 virulence factors. High homology was observed with previously identified human and swine strains in the same area. …”
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Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization by Gaetano Maugeri, Maddalena Calvo, Dafne Bongiorno, Dalida Bivona, Giuseppe Migliorisi, Grete Francesca Privitera, Guido Scalia, Stefania Stefani

“…This study documents emerging invasive sequence types such as ST101, ST307, and ST395, mainly revealing <i>blaNDM</i> or <i>blaKPC</i> genes, along with siderophores and hyperproduction capsule markers as virulence factors. Most of the detected factors are presumably related to a specific plasmid content, which are extremely varied and rich. …”
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The Fem cell-surface signaling system is regulated by ExsA in Pseudomonas aeruginosa and affects pathogenicity by Yanqi Li, Anjali Bhagirath, Sara Badr, Pansong Zhang, Lin Chen, Maryam Dadashi, Michael G. Surette, Kangmin Duan

“…Intriguingly, the Fem system also influenced virulence factors in P. aeruginosa, including the quorum sensing systems, pyocyanin production, biofilm formation, and the type six secretion systems (T6SSs). …”
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Detection of Francisellaceae and the differentiation of main European F. tularensis ssp. holarctica strains (Clades) by new designed qPCR assays by Kristin Köppen, Kerstin Rydzewski, Julia Zajac, Marwah Al-Senwi, Sema Evcimen, Darius Schulze, Daniela Jacob, Klaus Heuner

“…In addition to these Francisella species, further genera of the family Francisellaceae have been described, such as Allofrancisella, Parafrancisella and Pseudofrancisella, but less is known about the distribution and putative virulence of these genera. The methods currently available were not made for a fast and easy detection of all these strains and genera of Francisellaceae. …”
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Inhibition and evasion of neutrophil microbicidal responses by Legionella longbeachae by Hannah E. Hanford, Christopher T. D. Price, Silvia Uriarte, Yousef Abu Kwaik

“…Overall, L. longbeachae employs unique virulence strategies to evade the major microbicidal processes of neutrophils.…”
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Emerging biofilm formation and disinfectant susceptibility of ESBL-producing Klebsiella pneumoniae by Hanan S. Khalefa, Amany A. Arafa, Dalia Hamza, Khaled A. Abd El-Razik, Zeinab Ahmed

“…The presence of K. pneumoniae in animal and environmental sources, along with the high prevalence of ESBL-mediated resistance and biofilm-associated virulence genes, underscores the zoonotic potential of this pathogen. …”
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Antimicrobial and anti-quorum sensing activities of giant fennel (ferula elaeochytris korovin) from the hatay region by Enis Fuat Tüfekci, Anfal Alkateeb, Sarah Akar, Orhan Çorum, Yasemin Çelik Altunoğlu, Mehmet Cengiz Baloğlu, Muammer Kiraz, Nilay Çöplü

“…Because the QS system performs a crucial part in the synthesis of virulence factors in bacteria. Numerous medicinal plants are known to have promising antimicrobial and anti-QS activities. …”
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A broad spectrum Shigella vaccine based on VirG53 – 353 multiepitope region produced in a cell-free system by Girmay Desalegn, Charlotte Abrahamson, K. Ross Turbyfill, Lucy Pill-Pepe, Leslie Bautista, Chitradevi S. Tamilselvi, Dylan Dunn, Neeraj Kapoor, Barbara Sullinger, Matheo Herrera, Edwin V. Oaks, Jeff Fairman, Marcela F. Pasetti

“…We previously reported the broad protective capacity of VirGα, a conserved surface-exposed domain of Shigella virulence factor. Seeking to refine the vaccine antigenic target and achieve scalable manufacturing compatible with Good Manufacturing Practices, we mapped linear B-cell epitopes spanning the entire VirG protein sequence by probing the immune reactivity of 10-mer peptides (overlapping 4–8 aa) with sera from Shigella-infected rhesus monkeys. …”
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A model of the artificial metastasis of human epidermoid carcinoma A431 in nude mice for examination of the oncolytic activity of vaccinia virus by G. V. Kochneva, A. A. Grazhdantseva, G. F. Sivolobova, A. V. Tkacheva, A. N. Shvalov, A. Yu. Unusova, E. I. Ryabchikova, S. V. Netesov

“…We used the attenuated recombinant vaccinia virus (VACV) VVdGF-GFP2 of the L-IVP strain (GenBank accession number KP233807), with deletion of two virulence genes: the virus growth factor and thymidine kinase, with the gene for the green fluorescent protein (GFP2) inserted in an area of the latter. …”
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Environmental microbial communications in gram-positive and gram-negative bacteria by P. Srikanth, D. Sivakumar, J. Nouri

“…This may result in coordinated behaviours, including the development of biofilms, the generation of virulence factors, or other group activities. Without the ability to detect and react to the presence of their neighbours, microbial communities would not be able to adjust to changing environmental conditions or carry out collective actions that are essential for survival. …”
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Edible plant Artemisia argyi H.Lév. &amp; Vaniot: An in vitro study on its anti-Helicobacter pylori effect by Meiyun Chen, Sheng Pan, Fengli Lin, Weixing Zhu, Baojie Li, Bing Zhou, Guimin Zhang, Zhong Feng, Meicun Yao

“…It demonstrated no antagonistic effects when combined with antibiotics and disrupted H. pylori morphology and structure, downregulated various virulence genes, and inhibited urease activity. In co-culture systems, A. argyi reduced H. pylori adhesion, urease levels, and NO production, indicating immunomodulatory activity. …”
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Major F plasmid clusters are linked with ColV and pUTI89-like marker genes in bloodstream isolates of Escherichia coli by Cameron J. Reid, Max L. Cummins, Steven P. Djordjevic

“…We hypothesise that their abundance in BSI E. coli is partially driven by carriage of backbone genes previously shown to contribute to virulence in models of bloodstream infection. Their carriage by pandemic E. coli STs suggests clonal expansion also plays a role in their success in BSI. …”
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High hemolytic activity in Staphylococcus aureus t1081/ST45 due to increased hla protein production and potential RNAIII-independent regulation by Yu-Tzu Lin, Ngoc-Niem Bui, Yu-Syuan Cheng, Cheng-Wen Lin, Chun-Li Lee, Tai-Fen Lee, Po-Ren Hsueh

“…Background: α-Hemolysin, encoded by hla, is a major virulence factor of Staphylococcus aureus. Sequence type (ST) 45 is a globally spread clone with increasing clinical prevalence in Taiwan. …”
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Isolation and Characterization of Colistin-Resistant <i>Enterobacteriaceae</i> from Foods in Two Italian Regions in the South of Italy by Rosa Fraccalvieri, Angelica Bianco, Laura Maria Difato, Loredana Capozzi, Laura Del Sambro, Stefano Castellana, Adelia Donatiello, Luigina Serrecchia, Lorenzo Pace, Donatella Farina, Domenico Galante, Marta Caruso, Maria Tempesta, Antonio Parisi

“…Furthermore, 56 antimicrobial resistance genes were identified, suggesting the genetic mechanisms underlying resistance to several antibiotic classes. Virulence gene analysis showed that <i>E. coli</i> and other isolates carried genes linked to pathogenicity, increasing the potential risk to public health. …”
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Poor Glycemic Control in Carbapenem-Resistant Klebsiella pneumoniae Infections: Impact on Epidemiological Features, Mortality Risks, and Polymyxin Resistance by Wang Q, Yan T, Ma C, Teng X, Shen C, Wang N, Yu K, Chu W, Zhou Q, Liu Z

“…Antibiotic sensitivity, capsular serotypes, and virulence genes were tested of the strains. Three clinically isolated CRKP strains were used to investigate the effect of glucose on bacterial capsule synthesis and the impact on bacterial resistance to polymyxin and serum killing.Results: Patients with poor glycemic control experienced more severe infections and had a higher likelihood of chronic kidney disease (CKD) and acute renal insufficiency compared to those with good glycemic control. …”
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Tracking the Prevalence of Antibiotic Resistance in <i>Enterococcus</i> Within the Spanish Poultry Industry: Insights from a One Health Approach by Josep Garcia-Llorens, Isaac Monroy, Jan Torres-Boncompte, Jose M. Soriano, Pablo Catalá-Gregori, Sandra Sevilla-Navarro

“…Mannitol fermentation tests revealed high metabolic capacity in <i>Enterococcus faecalis</i> (99.25%) and <i>Enterococcus faecium</i> (96.88%), associated with adaptability and virulence potential. Genotypic analysis showed a very low prevalence of <i>vanB</i> and <i>vanC</i> genes. …”
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NEW MID-SEASON SPRING BARLEY CULTIVAR OMSKY 101 by P. N. Nikolaev, O. A. Yusova, N. I. Aniskov, I. V. Safonova, J. V. Ryapolova

“…‘Omsky 100’. Loose smut virulence rate was medium (23.1%), which is level with the reference but exceeds ‘Omsky 100’. …”
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