A systematic screening assay identifies efficient small guide RNAs for CRISPR activation by Elin Arvidsson, Diana Duarte Lobo, Diana Duarte Lobo, Ermelinda Sabarese, Fabio Duarte, Rui Jorge Nobre, Rui Jorge Nobre, Rui Jorge Nobre, Luis Quintino, Cecilia Lundberg

“…Correlation analysis of gene activation relative to sgRNA binding site distance to transcription start-site or nearby transcription factor binding sites failed to detect common characteristics influencing gene activation in the selected promoter regions. …”
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CAFs-released exosomal CREB1 promotes cell progression and immune evasion in thyroid cancer via the positive regulation of CCL20 by Chen Zheng, Hu Hei, Yifei Zhai, Wenbo Gong, Runfang Zhang, Songtao Zhang

“…The cAMP response element binding protein 1 (CREB1) acts as a transcription factor to participate in cancer development. …”
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All-Trans Retinoic Acid Inhibits Bone Marrow Mesenchymal Stem Cell Commitment to Adipocytes via Upregulating FRA1 Signaling by Linjun Xie, Liying Zou, Jie Chen, Youxue Liu

“…The expression level of FRA1 (FOS like 1, AP-1 transcription factor subunit) in atRA-treated BMSCs increased, suggesting that atRA-mediated inhibition of BMSCs adipogenesis involves FRA1. …”
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Maintenance of a Schwann-Like Phenotype in Differentiated Adipose-Derived Stem Cells Requires the Synergistic Action of Multiple Growth Factors by Alice E. Mortimer, Alessandro Faroni, Mahmut A. Kilic, Adam J. Reid

“…However, GGF-2 alone increased the expression of Krox20, the main transcription factor involved in myelination, relative to those cells treated with all stimulating factors. …”
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Single-cell transcriptome atlas of male mouse pituitary across postnatal life highlighting its stem cell landscape by Silke De Vriendt, Emma Laporte, Berkehür Abaylı, Julie Hoekx, Florian Hermans, Diether Lambrechts, Hugo Vankelecom

“…Importantly, we functionally validated transcriptomic findings using pituitary stem cell organoids, revealing roles for Krüppel-like transcription factor 5 (KLF5), activator protein-1 (AP-1) complex and epidermal growth factor (EGF) pathways in pituitary stem cell regulation. …”
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Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes by Li Bo, Tan Jun, Zou Bosheng, Liu Xiaojia, Yu Yiling

“…Normal primary HFM were isolated and cultured till logarithmic period of second passage, then treated with different concentrations of PCA (0.1–200 μmol L−1) to study the cell proliferation, melanin contents, tyrosinase activity and protein and mRNA expression of melanogenic genes (tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF)) in the cultured HFM. In addition, we have also measured the contents of superoxide dismutase (SOD) and glutathione (GSH) in PCA treated HFM. …”
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Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice by Chian-Jiun Liou, You-Rong Lai, Ya-Ling Chen, Yi-Hsien Chang, Zih-Ying Li, Wen-Chung Huang

“…In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. …”
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A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells by Sorina Dinescu, Bianca Galateanu, Eugen Radu, Anca Hermenean, Adriana Lungu, Izabela Cristina Stancu, Dana Jianu, Tudorita Tumbar, Marieta Costache

“…Moreover, we find that after exposure to prochondrogenic conditions a key transcription factor known to induce chondrogenesis, namely, Sox9, is highly expressed in our hADSCs/G-A-PAA bioconstruct, along with cartilage specific markers such as collagen type II, CEP68, and COMP extracellular matrix (ECM) components. …”
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Next generation thiazolyl ketone inhibitors of cytosolic phospholipase A2 α for targeted cancer therapy by Felicity J. Ashcroft, Asimina Bourboula, Nur Mahammad, Efrosini Barbayianni, Astrid J. Feuerherm, Thanh Thuy Nguyen, Daiki Hayashi, Maroula G. Kokotou, Konstantinos Alevizopoulos, Edward A. Dennis, George Kokotos, Berit Johansen

“…Consistent with these results, we show that elevated intracellular reactive oxygen species and activating transcription factor 4 target gene expression precede cell death in AVX420-sensitive T-cell acute lymphoblastic leukemia cells. …”
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Fenofibrate Enhances the In Vitro Differentiation of Foxp3+ Regulatory T Cells in Mice by Zhou Zhou, Ying Liang, Yanxiang Gao, Wei Kong, Juan Feng, Xian Wang

“…In addition, PPAR𝛼 could not upregulate the promoter activity of the Treg-specific transcription factor Foxp3. Fenofibrate might exert its function by enhancing Smad3 phosphorylation, a critical signal in Treg differentiation, via Akt suppression. …”
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Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition by Sajida Parveen, Kashif ur Rehman Khan, Shahid Muhammad Iqbal, Hanan Y. Aati, Areej M. Al-taweel, Liaqat Hussain, Musaddique Hussain, Musaddique Hussain

“…ELISA testing on BALF found that the plant extract efficiently reinstated superoxide dismutase, total anti-oxidant capacity, malondialdehyde, and total oxidative stress. qRT-PCR indicated a decline in the endotoxin-induced overproduction of pro-inflammatory markers, oxidative stress, transcription factor, and downregulated antioxidant potential in extract-treated groups. …”
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Palm Tocotrienol Activates the Wnt3a/β-Catenin Signaling Pathway, Protecting MC3T3-E1 Osteoblasts from Cellular Damage Caused by Dexamethasone and Promoting Bone Formation by Norfarahin Abdullah Sani, Nur Aqilah Kamaruddin, Ima Nirwana Soelaiman, Kok-Lun Pang, Kok-Yong Chin, Elvy Suhana Mohd Ramli

“…The expression of Wnt3a, β-catenin, collagen 1α1, alkaline phosphatase, osteocalcin, low-density lipoprotein receptor-related protein 6, and runt-related transcription factor-2 were significantly increased in the PTT-treated groups compared to the vehicle control group, especially at 0.5 µg/mL of PTT (<i>p</i> < 0.05) and on day 6 of treatment. …”
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KLF12 Aggravates Angiotensin II‐Induced Cardiac Remodeling in Male Mice by Transcriptionally Inhibiting SMAD7 by Min Hu, Shi‐Yu Huang, Yi‐Peng Gao, Yu‐Xin Hu, Sha‐Sha Wang, Teng Teng, Xiao‐Feng Zeng, Qi‐Zhu Tang

“…The KLF family of transcription factors plays crucial roles in heart injury. …”
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Curcumin Improves Epithelial Barrier Integrity of Caco-2 Monolayers by Inhibiting Endoplasmic Reticulum Stress and Subsequent Apoptosis by Xinxin Zhou, Mengting Ren, Jinpu Yang, Hanghai Pan, Mosang Yu, Feng Ji

“…Furthermore, curcumin administration greatly blocked the protein kinase-like endoplasmic reticulum kinase- (PERK-) eukaryotic translation initiation factor 2α- (eIF2α-) activating transcription factor 4- (ATF4-) CHOP signaling pathway. Curcumin enhanced intestinal epithelial barrier integrity in the in vitro coculture model by upregulating TJ protein expressions and reducing intestinal epithelial cell apoptosis. …”
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The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla by Chen Zhang, Xiao Han, Yuncun Liang, Huina Liu, Zhipeng Fan, Jianpeng Zhang

“…By an alkaline phosphatase (ALP) activity assay, Alizarin red staining, real-time RT-PCR, and western blot analysis, we found that KDM3B enhanced the ALP activity and mineralization of SCAPs and promoted the expression of runt-related transcription factor 2 (RUNX2), osterix (OSX), dentin sialophosphoprotein (DSPP), and osteocalcin (OCN). …”
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Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells by Yi-Lin Cheng, Yee-Shin Lin, Chia-Ling Chen, Shu-Wen Wan, Yi-Dan Ou, Chia-Yi Yu, Tsung-Ting Tsai, Po-Chun Tseng, Chiou-Feng Lin

“…Infection with dengue virus (DENV) causes an increase in proinflammatory responses, such as nitric oxide (NO) generation and TNF-α expression; however, the molecular mechanism underlying this inflammatory activation remains undefined, although the activation of the transcription factor NF-κB is generally involved. In addition to TNF-α production in DENV-infected murine macrophage RAW264.7 cells, inducible NO synthase was transcriptionally and posttranslationally elevated and accompanied by NO generation. …”
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Genetic Variations and mRNA Expression of NRF2 in Parkinson’s Disease by Caroline Ran, Karin Wirdefeldt, Lovisa Brodin, Mehrafarin Ramezani, Marie Westerlund, Fengqing Xiang, Anna Anvret, Thomas Willows, Olof Sydow, Anders Johansson, Dagmar Galter, Per Svenningsson, Andrea Carmine Belin

“…Nuclear factor erythroid 2-like 2 (NRF2) encodes a transcription factor regulating mechanisms of cellular protection and is activated by oxidative stress. …”
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Anthracyclines disaggregate and restore mutant p63 function: a potential therapeutic approach for AEC syndrome by Fabiana Boncimino, Ludovica D’Auria, Kristina Todorova, Sabina Y. van der Zanden, Jacques Neefjes, Anna Mandinova, Caterina Missero, Stefano Sol

“…Abstract Ankyloblepharon-Ectodermal Defects-Cleft Lip/Palate (AEC) syndrome is a rare genetic disorder caused by mutations in the TP63 gene, which encodes a transcription factor essential for epidermal gene expression. …”
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Novel insights into immunopathogenesis and crucial biomarkers between primary open‐angle glaucoma and systemic lupus erythematosus by Yixian Liu, Mengling You, Zhou Zeng, Jing Wang, Rong Rong, Xiaobo Xia

“…Additionally, machine learning identified five biomarkers, and we established associated transcription factors and miRNA regulatory networks. Immune infiltration analysis indicated an elevated presence of immune cells, including macrophages, T cells, and B cells, in both conditions. …”
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Activation of PPARγ Protects Obese Mice from Acute Lung Injury by Inhibiting Endoplasmic Reticulum Stress and Promoting Mitochondrial Biogenesis by Yin Tang, Ke Wei, Ling Liu, Jingyue Ma, Siqi Wu, Wenjing Tang

“…Conversely, expression of the mitochondrial biogenesis-related proteins peroxisome proliferator-activated receptor γ coactivator 1 (PGC-1α), nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TFAM) increased. Furthermore, activation of PPARγ is associated with decreased levels of lung inflammation and epithelial apoptosis and increased expression of adiponectin (APN) and mitofusin2 (MFN2). …”
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