Codon optimized influenza H1 HA sequence but not CTLA-4 targeting of HA antigen to enhance the efficacy of DNA vaccines in an animal model by Dito Anurogo, Chia-Yuan Chen, Chu-Chi Lin, Jeanne Adiwinata Pawitan, Daniel W. Qiu, J. Timothy Qiu

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Single cell RNA sequencing reveals human tooth type identity and guides in vitro hiPSC derived odontoblast differentiation (iOB) by Sesha Hanson-Drury, Sesha Hanson-Drury, Sesha Hanson-Drury, Anjali P. Patni, Anjali P. Patni, Anjali P. Patni, Anjali P. Patni, Deborah L. Lee, Deborah L. Lee, Deborah L. Lee, Ammar Alghadeer, Ammar Alghadeer, Ammar Alghadeer, Ammar Alghadeer, Yan Ting Zhao, Yan Ting Zhao, Yan Ting Zhao, Devon Duron Ehnes, Devon Duron Ehnes, Devon Duron Ehnes, Vivian N. Vo, Vivian N. Vo, Vivian N. Vo, Sydney Y. Kim, Sydney Y. Kim, Druthi Jithendra, Druthi Jithendra, Ashish Phal, Ashish Phal, Ashish Phal, Natasha I. Edman, Natasha I. Edman, Natasha I. Edman, Natasha I. Edman, Thomas Schlichthaerle, Thomas Schlichthaerle, David Baker, David Baker, David Baker, Jessica E. Young, Jessica E. Young, Julie Mathieu, Julie Mathieu, Hannele Ruohola-Baker, Hannele Ruohola-Baker, Hannele Ruohola-Baker, Hannele Ruohola-Baker, Hannele Ruohola-Baker

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High throughput single cell metagenomic sequencing with semi-permeable capsules: unraveling microbial diversity at the single-cell level in sewage and fecal microbiomes by Meilee Ling, Judit Szarvas, Vaida Kurmauskaitė, Vaidotas Kiseliovas, Rapolas Žilionis, Rapolas Žilionis, Baptiste Avot, Baptiste Avot, Patrick Munk, Frank M. Aarestrup

Subjects: “…single cell sequencing…”
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Germline Mutation in KIF1Bβ Gene Associated with Loss of Heterozygosity: Usefulness of Next-Generation Sequencing in the Genetic Screening of Patients with Pheochromocytoma by Giuseppina De Filpo, Elisa Contini, Viola Serio, Andrea Valeri, Massimiliano Chetta, Daniele Guasti, Daniele Bani, Massimo Mannelli, Elena Rapizzi, Michaela Luconi, Mario Maggi, Tonino Ercolino, Letizia Canu

“…We reported a case presentation and next-generation sequencing analysis supported by in silico studies and evaluation of mitochondrial status in KIF1Bβ tissue. …”
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Detection of colorectal‐cancer‐associated bacterial taxa in fecal samples using next‐generation sequencing and 19 newly established qPCR assays by Thulasika Senthakumaran, Tone M. Tannæs, Aina E. F. Moen, Stephan A. Brackmann, David Jahanlu, Trine B. Rounge, Vahid Bemanian, Hege S. Tunsjø

Subjects: “…16S rRNA amplicon sequencing…”
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Single-cell RNA sequencing reveals intrahepatic signature related to pathobiology of duck hepatitis A virus type 3 (DHAV-3) infection by Yunsheng Zhang, Dingbang Ding, Shaofei Li, Qiong Pan, Jinlong Ru, Haonan Zhao, Guang Yao, Jie Wei, Shuaiqin Wang, Shuisheng Hou, Xia Wang

“…To dissect the molecular characteristics associated with pathobiology of ducklings to DHAV-3, we applied single-cell RNA-sequencing approach to profile the transcriptome of 1.4 million cells from 14 livers of DHAV-3 susceptible (S) and resistant (R) ducklings during viral infection and 4 uninfected healthy controls. …”
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Improved Predictability of Diagnosis and Prognosis Using Serum‐ and Tissue‐Derived Extracellular Vesicles From Bulk mRNA Sequencing in Pancreatic Ductal Adenocarcinoma by Qian Zhu, Zhang Chen, Ming Tian, Xin Yan, Xiangdong Gongye, Zhicheng Liu, Anbang Zhao, Zhiyong Yang, Yufeng Yuan

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Diversity of bacterial symbionts associated with the tropical plant bug Monalonion velezangeli (Hemiptera: Miridae) revealed by high-throughput 16S-rRNA sequencing by Navarro-Escalante, Lucio, Benavides, Pablo, Acevedo, Flor E.

“…Based on PCR screening and DNA sequencing of the wsp gene, Wolbachia infection was confirmed in almost 80% of samples, and represented by two different isolates or strains within the supergroup B. …”
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The Investigation of Nonavailability of Passive Safety Systems Effects on Small Break LOCA Sequence in AP1000 Using RELAP5 MOD 4.0 by Anwar Hussain, Amjad Nawaz

“…However, the unavailability or failure to operate of any of the passive safety systems will change the accident sequence and may affect reactor safety. The analysis in this study is based on some hypothetical scenarios, in which the passive safety system failure is considered during the loss of coolant accidents. …”
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Investigation of interleukin-4 (IL-4) and leukotriene C4 synthase (LTC4S) genes in patients with asthma by next-generation sequencing analysis by Muhammet Mesut Nezir Engin, Önder Kılıçaslan, Recep Eröz, Öner Özdemir, Ramazan Temizkan, Kenan Kocabay

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Assessment of XCI skewing and demonstration of XCI escape region based on single-cell RNA sequencing: comparison between female Grave’s disease and control by In-Cheol Baek, Soo Yeun Sim, Byung-Kyu Suh, Tai-Gyu Kim, Won Kyoung Cho

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Integrative analysis of single-cell and transcriptome sequencing with experimental validation reveals PKHD1L1 as a novel biomarker in lung adenocarcinoma by Xiangqian Zhang, Jie Wang, Hanyang Su, Xiaojin Liu

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One Step Ahead Prediction of Ozone Concentration for Determination of Outdoor Air Quality Level by Ercan Avşar, Waleed Mahmood

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Comprehensive analysis of scRNA-seq and bulk RNA-seq reveals the non-cardiomyocytes heterogeneity and novel cell populations in dilated cardiomyopathy by Siyu He, Chunyu Li, Mingxin Lu, Fang Lin, Sangyu Hu, Junfang Zhang, Luying Peng, Li Li

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Single-cell transcriptomics in thyroid eye disease by Sofia Ahsanuddin, Albert Y. Wu

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Study on gene expression in the liver at various developmental stages of human embryos by Hanqing Chen, Tingting Li, Ming Cai, Zhiqi Huang, Jianjun Gao, Hongping Ding, Minmin Li, Weiyu Guan, Jinpeng Chen, Wenran Wang, Chunhong Li, Jianwu Shi

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Galaxy @Sciensano: a comprehensive bioinformatics portal for genomics-based microbial typing, characterization, and outbreak detection by Bert Bogaerts, Julien Van Braekel, Alexander Van Uffelen, Jolien D’aes, Maxime Godfroid, Thomas Delcourt, Michael Kelchtermans, Kato Milis, Nathalie Goeders, Sigrid C. J. De Keersmaecker, Nancy H. C. Roosens, Raf Winand, Kevin Vanneste

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Metagenomics approaches for studying the human microbiome by Biniam Moges, Degisew Yinur Mengistu

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Genetics of Primary Adrenal Insufficiency Beyond CAH in Saudi Arabian Population by Mohamed H. Al‐Hamed, Alya Qari, Lamya Alrayes, Mohammed Alotaibi, Zainab Al Masseri, Afaf Alotaibi, Abdullah AlAshwal, Zuhair N. AlHassnan, Afaf Alsagheir

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Polymorphism of <i>Sdr</i> genes regulating seed dormancy in <i>Triticum persicum</i> Vav. and <i>Triticum aethiopicum</i> Jakubz. by M. S. Bazhenov, E. D. Guseva, V. S. Rubets

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