A Case Report of Chlamydia psittaci Infective Endocarditis Complicated With Pneumonia by Dongmei Zhao, Li Zhang, Qiulin Sun, Jun Cheng

“…A rare case of infective endocarditis complicated by pneumonia caused by Chlamydia psittaci was reported, which was diagnosed using metagenomic next-generation sequencing (mNGS). The patient recovered after receiving appropriate anti-infective treatment. …”
Get full text

T-PLL Presenting with an Indolent Course by Arsa Thammahong, Narittee Sukswai, Chantana Polprasert

“…T-cell receptor clonality assessment by next-generation sequencing revealed a dominant clone in the ß-chain constant region. …”
Get full text

Characterization of CO1 and 18S rDNA genes from Indonesian native ornamental shrimp Caridina boehmei cultured in Jakarta by Ekky Ilham Romadhona, Novi Megawati, Handang Widantara, Sutanti Sutanti, Arif Rahman Ardiansyah, Kiki Mariya Dewi, Annisa Fitri Larassagita, Aditia Firman, Moh Risky, Rahmat Rahmat, Ayub Ayub

“…The CO1 sequencing produced fragments consisting of 25.84% adenine, 20.95% cytosine, 19.11% guanine, and 34.10% thymine. …”
Get full text

Aminoglycoside tolerance in Vibrio cholerae engages translational reprogramming associated with queuosine tRNA modification by Louna Fruchard, Anamaria Babosan, Andre Carvalho, Manon Lang, Blaise Li, Magalie Duchateau, Quentin Giai Gianetto, Mariette Matondo, Frederic Bonhomme, Isabelle Hatin, Hugo Arbes, Céline Fabret, Enora Corler, Guillaume Sanchez, Virginie Marchand, Yuri Motorin, Olivier Namy, Valérie de Crécy-Lagard, Didier Mazel, Zeynep Baharoglu

Get full text

Advancing poliovirus eradication: lessons learned from piloting direct molecular detection of polioviruses in high-risk and priority geographies by Paula L. Marcet, Brandon Short, Ashley Deas, Hong Sun, Chelsea Harrington, Shahzad Shaukat, Muhammad Masroor Alam, Marycelin Baba, Adedayo Faneye, Prossy Namuwulya, Lea Necitas Apostol, Tamer Elshaarawy, John Kofi Odoom, Peter Borus, Shelina Moonsamy, Yogolelo Riziki, Marie Claire Endegue Zanga, Mesfin Tefera, Anfumbom K. W. Kfutwah, Salmaan Sharif, Varja Grabovac, Cara C. Burns, Nancy Gerloff

“…DD-ITD implementation would entail building proficiency in advanced molecular laboratory techniques and data analysis, and increased demand for confirmatory sequencing.IMPORTANCESurveillance of acute flaccid paralysis (AFP) and sensitive poliovirus detection are key components of the WHO Global Polio Eradication Strategy. …”
Get full text

Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease by Zhen Zhu, Guangxin Luan, Song Wu, Yiyi Song, Shuang Shen, Kaiyue Wu, Shengnan Qian, Weiping Jia, Jun Yin, Tao Ren, Jianping Ye, Li Wei

“…Single-nucleus RNA sequencing (snRNA-seq) technology is a high-throughput sequencing analysis technique for RNA at the level of a single cell nucleus. …”
Get full text

Congenital muscular dystrophies and myopathies: the leading cause of genetic muscular disorders in eleven Chinese families by Bin Mao, Xiaoling Cai, Na Lin, Yulin Jiang, Na Hao, Yifang Dai, Danhua Guo, Deqin He, Huili Xue, Lingji Chen, Qianqian He, Min Zhang, Meihuan Chen, Hailong Huang, Liangpu Xu

“…Nanopore sequencing and optical genome mapping were applied for detecting complicated genome rearrangements. …”
Get full text

Spatiotemporal and genomic analysis of carbapenem resistance elements in Enterobacterales from hospital inpatients and natural water ecosystems of an Irish city by Mark Maguire, Carlos Serna, Natalia Montero Serra, Aneta Kovarova, Louise O’Connor, Niamh Cahill, Brigid Hooban, Niall DeLappe, Wendy Brennan, Genevieve Devane, Martin Cormican, Dearbháile Morris, Simone C. Coughlan, Georgios Miliotis, Bruno Gonzalez-Zorn, Liam P. Burke

“…We carried out whole-genome sequencing, combining short- and long-sequencing reads to enable complete characterization of CPE isolated from patients, wastewaters, and natural waters between 2018 and 2020 in Galway, Ireland. …”
Get full text

Atezolizumab plus bevacizumab in patients with unresectable or metastatic mucosal melanoma: 3‐year survival update and multi‐omics analysis by Jie Dai, Tianxiao Xu, Lifeng Li, Meiyu Fang, Jing Lin, Jun Cao, Xue Bai, Caili Li, Xiaoting Wei, Junjie Gu, Yaoyao Liu, Xuan Gao, Xuefeng Xia, Jun Guo, Yu Chen, Lili Mao, Lu Si

“…Available samples underwent whole exome sequencing, transcriptome sequencing and targeted bisulphite sequencing to assess correlations with clinical outcomes. …”
Get full text

Ophiostomatoid fungi associated with pine bark beetles and infested pines in south-eastern Australia, including Graphilbum ipis-grandicollis sp. nov. by Conrad Trollip, Angus J. Carnegie, Quang Dinh, Jatinder Kaur, David Smith, Ross Mann, Brendan Rodoni, Jacqueline Edwards

“…This study also provides an early example of whole genome sequencing (WGS) approaches replacing traditional PCR-based methods for taxonomic surveys. …”
Get full text

No microorganism was detected in amniotic fluid of healthy pregnancies from the second trimester to the delivery by Yu Liu, Jingmei Ma, Xiang Li, Haijuan Zhao, Qubo Ai, Lanying Zhang, Yulong Tong, Lingzhen Meng, Huixia Yang

“…While numerous studies have attempted to determine the presence or absence of intrauterine bacteria, the majority of them have drawn conclusions based on sequencing data from maternal or infant samples taken at a single time point. …”
Get full text

Pathogenicity of avian reovirus variant in the immune organs of broiler chicks by Haiyang Yu, Yudong Zhu, Qiong Wu, Wanjun Zhao, You Wang, Dong Wang, Huaguang Lu, Youxiang Diao, Yijing Li, Yi Tang

“…In this study, an ARV strain (Chicken/XJ/China/2022/01/16) was isolated from arthritic broiler chicks in Xinjiang province and whole genome sequencing of the ARV strain was performed using next generation sequencing (NGS) technology, and sequence analysis showed that the strain was a type V variant of the ARV gene. …”
Get full text

The Development of a One-Step PCR Assay for Rapid Detection of an Attenuated Vaccine Strain of Duck Hepatitis Virus Type 3 in Korea by Cheng-Dong Yu, Jong-Yeol Park, Sang-Won Kim, Yu-Ri Choi, Se-Yeoun Cha, Hyung-Kwan Jang, Min Kang, Bai Wei

“…The clinical sample results were completely consistent with those obtained using nested PCR detection and gold-standard sequencing. In summary, we successfully developed a rapid, one-step MAMA-PCR method that is more suitable for clinical diagnosis than traditional sequencing methods.…”
Get full text

Comprehensive bioinformatics analysis identifies metabolic and immune-related diagnostic biomarkers shared between diabetes and COPD using multi-omics and machine learning by Qianqian Liang, Yide Wang, Zheng Li, Zheng Li, Zheng Li, Zheng Li

“…Using xCell, we investigated the relationship between shared biomarkers and immune infiltration in diabetes and COPD. Single-cell sequencing, clinical samples, and animal models were used to confirm the robustness of shared biomarkers.ResultsCross-analysis identified 186 shared DEGs between diabetes and COPD patients. …”
Get full text

MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity by Yong Fang, Tianhang Huang, Jian Wu, Xieqi Luo, Fengjiao Fan, Peng Li, Jian Ding, Xinyang Sun

“…In this study, RNA-sequencing and miRNA-sequencing were used to independently identify differentially expressed mRNAs and small RNAs profi les in Pb2+-treated primary fetal rat cortical neurons and then the correlated miRNA-mRNA target pairs were obtained. …”
Get full text

Characterization and pathogenicity of a novel avian orthoreovirus in China by Shunyan Chen, Jialin Yang, Li Li, Yawei Guo, Shenghua Yang, Zetao Su, Sucan Zhao, Xuesong Li, Wencheng Lin, Yunping Du, Lijuan Yin, Lianxiang Wang, Feng Chen

“…IntroductionAvian orthoreovirus (ARV) is a significant pathogen causing viral arthritis, leading to substantial economic losses in the poultry industry worldwide.MethodsA novel ARV strain, designated FJ202311, was isolated from a broiler farm in Fujian Province, China. Whole-genome sequencing was conducted using next-generation sequencing with MGI technology, and phylogenetic analysis of the sigma C amino acid sequence was performed. …”
Get full text

A Highly Efficient System for Separating Glandular and Non-glandular Trichome of Cucumber Fruit for Transcriptomic and Metabolomic Analysis by Lei Sun, Zhongxuan Feng, Fang Wang, Yu Qi, Menghang An, Lin Yang, Min Feng, Mingqi Wang, Huazhong Ren, Xingwang Liu

“…However, current methods for separating trichomes encounter challenges such as low efficiency and insufficient accuracy, limiting their applicability in multi-omics sequencing studies. This protocol introduces an efficient system designed for the precise separation of glandular and non-glandular trichomes from cucumber fruit. …”
Get full text

Rare coinheritance of hemoglobin vancleave with severe beta-thalassemia mutation in a patient with secondary erythrocytosis by Nur Aisyah Aziz, Nurul Hidayah Musa, Melina Mathews, Komalah Thevii Rajenderan, Faidatul Syazlin Abdul Hamid, Syahzuwan Hassan, Syahira Lazira Omar, Wan Nurul Afiqha Binti Wan Yusoff, Melanie Ling Binti Mohd Din, Nurul Amira Binti Jamaludin, Wan Rohani Wan Taib, Ezalia Esa, Norafiza Mohd Yasin

“…The diagnosis of Hb Vancleave, in combination with codon 41/42 (-TTCT) (NM_000518.5:c.126_129del; dbSNP: rs80356821), was confirmed by direct sequencing. This report highlights the importance of sequencing in the differential diagnosis of beta-thalassemia syndrome in Malaysia.…”
Get full text

Characterization of volatile compounds and microbial diversity of Arabica coffee in honey processing method based on different mucilage retention treatments by Faguang Hu, Haohao Yu, Xingfei Fu, Zhongxian Li, Wenjiang Dong, Guiping Li, Yanan Li, Yaqi Li, Bingqing Qu, Xiaofei Bi

“…This study combined HS-SPME-GC–MS and high-throughput sequencing to explore how honey-processing methods with varying mucilage retentions impact volatile compounds and microbial communities in green coffee beans. …”
Get full text

Shenlian (SL) Decoction, a Traditional Chinese Medicine Compound, May Ameliorate Blood Glucose via Mediating the Gut Microbiota in db/db Mice by Rui-xi Sun, Wei-jun Huang, Yao Xiao, Dou-dou Wang, Guo-hua Mu, He Nan, Bo-ran Ni, Xiao-qiang Huang, Hsuan-chuan Wang, Yi-fan Liu, Qiang Fu, Jin-xi Zhao

“…To explore the effect of SL decoction on intestinal microbiota, gut microbiota of mice was analyzed by sequencing the gut bacterial 16S rRNA V3+V4 region and metagenomics. …”
Get full text