Effects of White Quinoa Polysaccharide on Regulation of Glucolipid Metabolism in Type 2 Diabetic Mice by Yanqing ZANG, Yingying CHUANG, Changyuan WANG, Yang CAO

“…The results of 16S rRNA sequencing revealed that the intake of WQP led to an increase in the abundance of the Bacteroidota and a decrease in the Firmicutes in the intestines of diabetic mice. …”
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Efficacy of 0.5% Levofloxacin and 5.0% Povidone-Iodine Eyedrops in Reducing Conjunctival Bacterial Flora: Metagenomic Analysis by Caixia Fan, Baoxia Yang, Yusen Huang

“…In conclusion, the metagenomic analysis using high-throughput sequencing provides a scientific way for evaluating the effectiveness of a disinfection method from the perspective of analyzing the composition and diversity of the conjunctival microbiome. …”
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Discovery of two novel Flavobacterium species with potential for complex polysaccharide degradation by Xu-Dong Lian, Yong Guan, Yue Jiang, Dong-Heui Kwak, Mi-Kyung Lee, Zhun Li

“…Through comprehensive analyses, including 16S rRNA gene sequencing, whole-genome sequencing, and detailed morphological, physiological, and chemotaxonomic characterizations, these strains have been identified as new species within the genus Flavobacterium. …”
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Prevalence and Subtype Analysis of Blastocystis hominis Isolated from Patients in the Northeast of Iran by Mitra Salehi, Jalal Mardaneh, Hamid Reza Niazkar, Mohammadhaasan Minooeianhaghighi, Elahe Arshad, Fateme Soleimani, Alireza Mohammadzadeh

“…Also, PCR was done on the positive samples, and sequencing and phylogenetic analysis were subsequently performed. …”
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Molecular Identification of Fusarium proliferatum from toenails: A case report by Sazan Jamal Gharib, Nawroz Abdul-razzak Tahir

“…For final identification sequencing of DNA has been carried out by [Macrogen Korea] using forward primer ITS1, The obtained consensus of query sequence was compared with the ITS DNA database on the BLAST homepage, aligned and diagnosed strain deposited to the GenBank and we received an accession number (MK112619). …”
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Altered Protein Structures and Neoepitopes in Lupus Neutrophils From Dysregulated Splicing of Messenger RNA by Rayan Najjar, Xiaoxing Wang, Jose Mario Bello Pineda, Hugh Alessi, Alison Bays, Robert K. Bradley, James N. Jarvis, Tomas Mustelin

“…RNA sequencing from SARS‐CoV2–infected individuals was used as an additional comparator. …”
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Technical considerations towards commercialization of porcine respiratory and reproductive syndrome (PRRS) virus resistant pigs by A. Mark Cigan, Pieter W. Knap

“…As traditional methods of classical breeding and selection for trait improvement are slow, recent progress in several areas of biology including (a) understanding host–pathogen interactions, (b) inexpensive and rapid DNA sequencing, and (c) robust gene editing like CRISPR-Cas provide geneticists tools to accelerate discovery and deployment of disease resistance alleles in livestock. …”
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Fetal medicine and current practice of prenatal screening by Akshatha Prabhu

“…The fetal sample is used for various genetic tests, such as chromosome microarray analysis (CMA), targeted clinical exome sequencing (CES), or whole exome sequencing (WES) depending upon the abnormality noted or the suspected genetic condition. …”
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Effect of age and sex differences on the abundances of neuronal, glial, and endothelial cells in non-diseased brain tissue. by Arttu Autio-Kimura, Tapio Nevalainen, Mikko Hurme

“…The relative abundance of excitatory and inhibitory neurons, astrocytes, oligodendrocytes, microglia, and endothelial cells was estimated from the RNA sequencing data using a deconvolution-based analysis. …”
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Network analysis reveals potential mechanisms that determine the cellular identity of keratinocytes and corneal epithelial cells through the Hox/Gtl2-Dio3 miRNA axis by Yanjie Guo, Weini Wu, Haoyu Chen, Xueqi Wang, Yi Zhang, Shuaipeng Li, Xueyi Yang

“…In this study, to investigate the molecular mechanisms determining the identity of keratinocytes and corneal epithelial cells, small RNA and mRNA sequencing analyses of these two cell types were performed. …”
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Tumor mutational burden quantification from targeted gene panels: major advancements and challenges by Sara Gandini, Laura Fancello, Pier Giuseppe Pelicci, Luca Mazzarella

“…TMB can be quantitated by a number of NGS-based sequencing technologies. Whole Exome Sequencing (WES) allows comprehensive measurement of TMB and is considered the gold standard. …”
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SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice by Chunxia Yu, Peng Zhang, Sujuan Liu, Yanmei Niu, Li Fu

“…Fecal microbiota composition and function were assessed by 16S rRNA sequencing. The sequencing results showed that SESN2−/− mice displayed differed microbiome profile from WT mice. …”
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Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia by Xiuchun Yang, Yijun Zong, Zhentian Zhang, Yan Zhao, Xueying Gao, Jie Zhang, Qian Hou, Renyi Li, Bing Xiao

“…Peripheral venous blood samples from patients with CAE were collected for RNA sequencing to identify differentially expressed genes (DEGs), followed by functional enrichment. …”
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Exploration of predictive markers associated with primary angle-closure glaucoma risk in companion dogs using genomic information by Ji Hyun Jun, Gwang Hyeon Lee, Zultsetseg Byambasuren, Hong Sik Kong

“…Sequencing analysis results for SNP validation revealed differences in frequency among breeds. …”
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Heme Oxygenase 1/Peroxisome Proliferator-Activated Receptor Gamma Pathway Protects Intimal Hyperplasia and Mitigates Arteriovenous Fistula Dysfunction by Regulating Oxidative Stres... by Tingfei Xie, Yunpeng Xu, Lecai Ji, Xiaolu Sui, Aisha Zhang, Yanzi Zhang, Jihong Chen

“…AVF model mice were established, and the vascular tissues from the arteriovenous anastomosis site were sent for mRNA sequencing. Differentially expressed mRNAs (DEmRNAs) were screened and subjected to functional analysis. …”
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Conservative mechanism through various rapeseed (Brassica napus L.) varieties respond to heavy metal (Cadmium, Lead, Arsenic) stress by Lingyu Li, Zhanhuang Fan, Qingqin Gan, Gang Xiao, Mingbao Luan, Rilong Zhu, Zhenqian Zhang

“…Nevertheless, its ability to tolerate heavy metal stress at the molecular level remains unclear.MethodsHere, with 7-day seedlings as raw materials, we investigated physiological and biochemical indexes, analyzed the transcriptome sequencing for different treated materials (control, 50×, and 100×), combined with the results of transcriptome and proteome sequencing of the near-isogenic lines (F338 and F335) to reveal the response mechanism to heavy metal stress. …”
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Exploring RNA modifications in infectious non-coding circular RNAs by Pavel Vopalensky, Anton Škríba, Michela Chiumenti, Lucia Ďuričeková, Anna Šimonová, Ondřej Lukšan, Francesco di Serio, Beatriz Navarro, Hana Cahova

“…ONT sequencing predicted three m6A positions. Employing orthogonal SELECT method, we confirmed m6A in two positions A353 and A360, which are highly conserved among CEVd variants. …”
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Genomic prediction of forage nutritive value in perennial ryegrass by Agnieszka Konkolewska, Michael Dineen, Rachel Keirse, Patrick Conaghan, Dan Milbourne, Susanne Barth, Aonghus Lawlor, Stephen Byrne

“…The objective of this study was to phenotype a training population and develop prediction models to assess the potential of predicting organic matter digestibility (OMD) and neutral detergent fiber (NDF) with genotyping‐by‐sequencing data. Methods Near infra‐red reflectance spectroscopy calibrations for OMD and NDF were developed and used to phenotype a spaced plant training population of n = 1606, with matching genotype‐by‐sequencing data, for developing genomic selection models. …”
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Single-cell landscape of dynamic changes in CD8+ T cells, CD4+ T cells and exhausted T cells in hepatocellular carcinoma by Rongqiang Liu, Jing Ye, Jianguo Wang, Wangbin Ma, Zhendong Qiu, Jia Yu, Weixing Wang

“…In this study, we investigated the value of T-cell-related genes in prognosis by single-cell sequencing data in hepatocellular carcinoma. Twelve cases of hepatocellular carcinoma single-cell sequencing were included in the study. …”
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Identifying novel heterozygous PI4KA variants in fetal abnormalities by Chen Cheng, Fan Yang, Xinlin Chen, Sheng Zhao

“…Novel PI4KA variants were detected through clinical Whole exon sequencing (WES) and validated by Sanger sequencing. …”
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