Cell Differentiation Trajectory Predicts Prognosis and Immunotherapeutic Response in Clear Cell Renal Cell Carcinoma by Jin Xu, Xi Chen, Yinyu Chen, Qiushuang Wang, Yingliang Jin, Huashuo Zhao

“…Primary single cell culture and single cell sequencing are novel research technologies. In this study, we explored the differentiation status of ccRCC cells and its significance in prognosis and immunotherapeutic response through bioinformatics. …”
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Somatic Mutaome Profile in Human Cancer Tissues by Nayoung Kim, Yourae Hong, Doyoung Kwon, Sukjoon Yoon

“…The Cancer Genome Atlas database includes large amounts of sequencing and omics data generated from diverse human cancer tissues. …”
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Wastewater metagenomics in Africa: Opportunities and challenges. by Stephen Kanyerezi, Fatma Zahra Guerfali, Abbas Abel Anzaku, Oluwasegun Adesina Babaleye, Tracey Calvert-Joshua, Julien Alban Nguinkal, Oluwaseun Paul Amoo, Chiraz Atri, Waqasuddin Khan, Iqra Saleh, M Imran Nisar, Arthur Shem Kasambula, Koketso Morapedi, Gerald Mboowa

“…These include variability in wastewater composition due to differing local customs, limited infrastructure for sequencing and data analysis, and a shortage of bioinformatics expertise. …”
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Genotypic Profiling of Bacillus cereus Recovered from Some Retail Foods in Ogun State, Nigeria, and Their Phylogenetic Relationship by Titilayo O. Adesetan, Moses O. Efuntoye, Olubukola O. Babalola

“…Thirty of the isolates were subjected to molecular identification by 16S rDNA sequencing. The thirty sequences were deposited in GenBank for accession number. …”
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Chromosome-level genome assembly of Fistularia commersonii (Syngnathiformes, Fistulariidae) by Zufa Ding, Cheng Wang, Yuting Qian, Wenjun Chen, Dan Yu, Yang Liu, Liandong Yang, Ying Wang

“…In this study, we achieve assembly of a chromosome-level genome for F. commersonii by harnessing the precision of PacBio HiFi sequencing in conjunction with the sophistication of Hi-C sequencing technologies. …”
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The curated Lactobacillus acidophilus NCFM genome provides insights into strain specificity and microevolution by Meichen Pan, Sarah O’Flaherty, Ashley Hibberd, Svetlana Gerdes, Wesley Morovic, Rodolphe Barrangou

“…Abstract Background The advent of next generation sequencing technologies has enabled a surge in the number of whole genome sequences in public databases, and our understanding of the composition and evolution of bacterial genomes. …”
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Chromosome-level genome assembly of a destructive leaf-mining moth Eriocrania semipurpurella alpina by Gui-fang Wang, Jin-wu He, Cheng Sun, Xue-yan Li, Pei-feng Qi, Dan-dan Feng, Cai-qing Yang, Ai-bing Zhang

“…BUSCO completeness values of 97.6%. 49.02% of the genome sequences were recognized as repetitive sequences, and a total of 22,581 protein-coding genes were annotated. …”
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Audiological alterations in resistance to thyroid hormone syndrome: emphasizing lifelong assessment by Alexandre Machado Silva de Oliveira, Viviane Motti, Jessica Casemiro, Pedro Resende Rende, Caio Leônidas Oliveira de Andrade, Luciene da Cruz Fernande, Helton Estrela Ramos

“…Genetic sequencing confirmed the THRB gene alteration (p.M442T) in three family members. …”
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Truncated SPAG9 as a novel candidate gene for a new syndrome: Coarse facial features, albinism, cataract and developmental delay (CACD syndrome) by Majid Alfadhel, Bashayr S. Alhubayshi, Muhammad Umair, Ahmed Alfaidi, Deemah Alwadaani, Essra Aloyouni, Safdar Abbas, Abdulkareem Al Abdulrahman, Mohammed Aldrees, Abeer Al Tuwaijri, Ruaa S. Alharithy, Abdulaziz Alajlan, Abdulrahman Alswaid, Saad Almohrij, Sultan Al-Khenaizan

“…Sanger sequencing of both families revealed perfect segregation of the identified variant in all family members. 3D protein modeling revealed substantial changes in the protein’s secondary structure. …”
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Multiplex-PCR technique to predict polymorphic antigens – M, N, S and s – and associations between their alleles and Mia-associated hybrid glycophorins by Thanaporn Kerdthip, Oytip Nathalang, Sarisa Chidtrakoon, Tanaporn Choychimplee, Dollapak Apipongrat, Kamphon Intharanut

“…Remarkably, multiplex PCR was well optimised, and the results aligned with serological phenotyping and DNA sequencing data with maximum accuracy and reliability; this confirmed our findings on its validity in predicting MNSs phenotypes. …”
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Cardiac Tamponade: A Rare Manifestation of Familial Mediterranean Fever by Abdolreza Malek, Tina Zeraati, Ariane Sadr-Nabavi, Niloofar Vakili, Mohammad Reza Abbaszadegan

“…Finally, the mutation found by whole exome sequencing was confirmed by direct Sanger sequencing revealing a heterozygote c.44G > C (p.Glu148Gln) mutation in exon 2, confirming the clinical diagnosis of familial Mediterranean fever. …”
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Mutational Profile and Retinal Phenotypes of PCARE-Related Cone-Rod Dystrophies in a Mexican Cohort by Víctor R. López-Rodríguez, Rocío Arce-González, Alan Martínez-Aguilar, Carlos E. Rodríguez-López, Sergio Groman-Lupa, M. Isabel Neria-González, Genaro Rodríguez-Uribe, Juan C. Zenteno

“…Genetic screening was performed either by gene panel sequencing or by exome sequencing. Results. According to the results of multimodal imaging and functional tests, all 14 patients were diagnosed with cone-rod dystrophy. …”
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Clinical follow-up of 2 families with glomerulopathy caused by COQ8B gene variants and literature review by Lei Zhang, Gentzon Hall, Peitong Han, Chunzhen Li, Jieyuan Cui

“…Pathogenic variants were identified in COQ8B in the exome studies and confirmed by direct sequencing.ResultsClinical exome sequencing revealed biallelic variants of the COQ8B gene in 2 families. …”
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Edaravone dexborneol alleviates pericyte-mediated fibrosis depositing extracellular matrix through TGF-β1/IL-11 in cerebral small vessel disease by Qingrui Duan, Zhiyang Liu, Yuxuan Xing, Haifeng Huang, Lin Zhu, Jiaxuan Liu, Peikun He, Guixian Ma, Yuhu Zhang, Kun Nie, Yuyuan Gao, Lijuan Wang

“…Subsequently, the results of sequencing were confirmed in both cellular and mouse model. …”
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Rapidly Fatal Postlaparoscopic Liver Infection from the Rarely Isolated Species Clostridium butyricum by Kenneth L. Muldrew

“…Liver examination at autopsy showed cystic spaces, necrosis, and spore-forming Gram-positive rods. 16sRNA gene sequencing of the cystic liver tissue identified the organism as C. butyricum.…”
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Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…The immunosuppressive effect of these two pathways on the tumor microenvironment was evaluated via RNA sequencing. Single-cell RNA sequencing was further applied to investigate the dysfunctional CD8+ T cells. …”
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Metagenomic insights into the development of microbial communities of straw and leaf composts by Anastasiia K. Kimeklis, Anastasiia K. Kimeklis, Grigory V. Gladkov, Olga V. Orlova, Tatiana O. Lisina, Alexey M. Afonin, Alexey M. Afonin, Tatiana S. Aksenova, Arina A. Kichko, Alla L. Lapidus, Evgeny V. Abakumov, Evgeny V. Abakumov, Evgeny E. Andronov

“…Using a comprehensive approach with chemical analyses of the substrates, amplicon, and full metagenome sequencing data, we have shown that such communities may be a source of identifying the highly effective decomposing species with novel PULs.…”
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Deciphering the therapeutic effects of Xiyanping injection: insights into pulmonary and gut microbiome modulation, SerpinB2/PAI-2 targeting, and alleviation of influenza a virus-in... by Tengwen Liu, Shuping Li, Xuerui Wang, Mingjiang Liu, Yuchen Wang, Jie Ying, Shuwen Zhang, Yan Lin, Ning Wang, Yungjing Bai, Lan Xie, Tengfei Chen, Quansheng Feng, Xiaolong Xu

“…In this study, we established an IAV infection mice model, and utilized 16 S rRNA sequencing, RNA sequencing, protein chips, and molecular docking, to investigate the mechanisms of XYP injection on altering pulmonary and gut microbiota, and identifying its target sites. …”
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A stratified two-stage tumor molecular profiling algorithm to identify clinically actionable molecular alterations in pancreatic cancer☆ by S. Hussung, D. Akhoundova, C. Pistoni, D. Lenggenhager, A. Töpfer, C. Pauli, B. Pestalozzi, C. Britschgi, M. Zoche, M. Rechsteiner, H. Moch, A. Weber, R. Fritsch

“…Stage 1 comprised immunohistochemistry for mismatch repair deficiency and targeted sequencing employing a 33-gene next-generation sequencing panel covering common PC drivers and DNA damage response genes. …”
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Deciphering the generation of heterogeneity in esophageal squamous cell carcinoma metastasis via single-cell multiomics analysis by Kaiwen Sheng, Jun Chen, Ruitang Xu, Haoqiang Sun, Ran Liu, Yongjie Wang, Wenwen Xu, Jiao Guo, Miao Zhang, Shuai Liu, Juan Lei, Yawen Sun, Yang Jia, Dianhao Guo

“…In conjunction, we integrated these data with 55 scRNA-seq datasets, ChIP-seq or CUT&Tag sequencing data, Hi-C sequencing data, bulk RNA-seq data, and bulk ATAC-seq data from ESCC cell lines to dissect the mechanisms underlying the heterogeneity of ESCC and tumor microenvironment (TME). …”
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