Intratumor heterogeneity: models of malignancy emergence and evolution by R. A. Ivanov, S. A. Lashin

“…Furthermore, single­cell sequencing methods have emerged as powerful tools for dissecting intratumoral heterogeneity and tracing clonal evolution. …”
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Spatial transcriptomics of healthy and fibrotic human liver at single-cell resolution by Brianna R. Watson, Biplab Paul, Raza Ur Rahman, Liat Amir-Zilberstein, Åsa Segerstolpe, Eliana T. Epstein, Shane Murphy, Ludwig Geistlinger, Tyrone Lee, Angela Shih, Jacques Deguine, Ramnik J. Xavier, Jeffrey R. Moffitt, Alan C. Mullen

“…Abstract Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of cell types and their heterogeneity within the human liver, but the spatial organization at single-cell resolution has not yet been described. …”
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Identification of a Novel Non-V600E BRAF Mutation in Papillary Thyroid Cancer by Marco Capezzone, Maja Rossi, Elisabetta Macerola, Silvia Cantara, Francesco Pepe, Eugenia Maria Morabito, Gilda Dalmazio, Sara Bardi, Agostino Ognibene, Massimo Alessandri, Gabriele Materazzi, Luigi De Napoli, Michele Cirianni, Liborio Torregrossa

“…The presence of this new variant of BRAF mutation was subsequently confirmed in the postoperative phase by direct Sanger sequencing. In conclusion, we report a new non-V600E variant previously undetected in papillary thyroid cancer. …”
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NLRP3 Is Expressed in the Spiral Ganglion Neurons and Associated with Both Syndromic and Nonsyndromic Sensorineural Deafness by Penghui Chen, Longxia He, Xiuhong Pang, Xiaowen Wang, Tao Yang, Hao Wu

“…Though a variety of targeted next-generation sequencing (NGS) panels has been recently developed to facilitate genetic screening of nonsyndromic deafness, some syndromic deafness genes outside the panels may lead to clinical phenotypes similar to nonsyndromic deafness. …”
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Mitochondrial DNA Structure in <i>Trypanosoma cruzi</i> by Alfonso Herreros-Cabello, Francisco Callejas-Hernández, Manuel Fresno, Núria Gironès

“…However, next-generation sequencing (NGS) methods have resolved these issues, enabling the complete sequencing of maxicircles from different strains. …”
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Genetic analysis of a serologically weak D phenotype caused by the p. R191G variant of the RHAG gene by ZHANG Xu, LI Xiaofeng, LI Jianping

“…[Results] The serological test results were weak D and normal Rh Ccee phenotypes. Normal sequence of RHD and RHCE genes, and a homozygous variation c. 572G>A (p. …”
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The First Infant Bloodstream Infection Caused by Pantoea dispersa in China: A Case Report and Literature Review by Yu W, Sun Z, Wang M, Li Z, Zhang C, Sun Y, Wang S

“…Through MALDI Biotyper and next-generation sequencing techniques, the pathogen was identified as P. dispersa. …”
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Ascophyllum nodosum and Fucus vesiculosus ameliorate restenosis via improving inflammation and regulating the PTEN/PI3K/AKT signaling pathway by Crystal Ngofi Zumbi, Chun-Hsu Pan, Hui-Yu Huang, Chieh-Hsi Wu

“…Meanwhile, according to the 16S rRNA gene sequencing analysis, supplementation with SE modulated gut microbiota composition in injured rats. …”
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Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. by Peng Du, Jianguo Wu, Jiayao Zhang, Shuqi Zhao, Hong Zheng, Ge Gao, Liping Wei, Yi Li

“…Our results provide a comparative analysis, via deep sequencing, of changes in the small RNA profiles and in the genes of RNA silencing machinery induced by different viruses in a natural and economically important crop host plant. …”
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Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host. by Martine Bangratz, Aurore Comte, Estelle Billard, Abdoul Kader Guigma, Guillaume Gandolfi, Abalo Itolou Kassankogno, Drissa Sérémé, Nils Poulicard, Charlotte Tollenaere

“…We aimed to decipher the distinct RYMV isolates that simultaneously infect a single rice plant and to sequence their genomes. To this end, we tested different sequencing strategies, and we finally combined direct cDNA ONT (Oxford Nanopore Technology) sequencing with the bioinformatics tool RVhaplo. …”
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STK3 Suppresses Ovarian Cancer Progression by Activating NF-κB Signaling to Recruit CD8+ T-Cells by Xiangyu Wang, Fengmian Wang, Zhi-Gang Zhang, Xiao-Mei Yang, Rong Zhang

“…RNA sequencing and gene set enrichment analysis (GSEA) were used to compare the transcriptome changes in the STK3 overexpression ovarian cancer and control cells. …”
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Diagnostic validity of the fifth edition of the Wechsler intelligence scales for children in characteristics Neuro-psychology of students with learning disability by Marzieh Shiri Aminlou, Parviz Sharifi Avaradi, Javad Khalatbari

“…The collected data were analyzed using logistic regression analysis and SPSS software (Version 23).The study finally revealed that vocabulary and information tests (from verbal comprehension index), Block design with time point (from Visual-spatial index),arithmetic (from fluid reasoning scale), number span (direct), number span (reverse), picture span, letter-digit sequencing and letter-number sequencing (from working memory scale),and coding, cancellation (random), cancellation (structural), (from Processing speed index), had diagnostic validity.The fifth version of the Wechsler IQ Scales for children has diagnostic validity in the neuropsychological characteristics of students with learning disabilities and can distinguish students with learning disabilities from normal students.…”
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Genetic Diversity of Human Enterovirus in Kazakhstan, during 2022 by Dinara Kamalova, Assel Akhmetova, Asylulan Amirgazin, Igor Sytnik, Viktoriya Rudenko, Gulzhan Yessimkhanova, Dinagul Bayesheva, Sergey Yegorov, Alexander Shevtsov

“…We then genotyped enteroviral subtypes associated with AM (n = 50) and HA (n = 9) using next-generation sequencing (NGS) on the viral protein 1 (VP1), followed up by whole-genome sequencing of the isolated viral species. …”
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Hypermethylation of Hif3a and Ifltd1 is associated with atrial remodeling in pressure-overload murine model by Keiko Abe, Tetsuo Sasano, Yurie Soejima, Haruhisa Fukayama, Shigeru Maeda, Tetsushi Furukawa

“…A comprehensive analysis using RNA-sequencing (RNA-Seq) and methyl-CpG-binding domain sequencing (MBD-Seq) in the left atrial tissue identified Hif3a and Ifltd1 as showing increased DNA methylation in their promoter regions and decreased RNA expression. …”
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CryoSCAPE: Scalable immune profiling using cryopreserved whole blood for multi-omic single cell and functional assays by Alexander T. Heubeck, Cole Phalen, Neel Kaul, Peter J. Wittig, Jessica Garber, Morgan Weiss, Palak C. Genge, Zachary Thomson, Claire Gustafson, Julian Reading, Peter J. Skene

“…The method was then optimized and verified for high sample throughput using fixed single cell RNA sequencing and liquid handling automation with a single batch of 60 cryopreserved whole blood samples. …”
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Molecular Assessment of Blastocystis hominis in Patients with Diabetes Mellitus in Balad City, Saladin Province, Iraq by Shereen Khalid Ismaiel, Abeer Abbas Ali, Akeel Hussein Ali Al-Assi

“…Thirty samples were further studied using PCR, and only 16 showed clear bands on the gel, whereas only eight samples were successfully sequenced. Conclusion: This study demonstrated that diabetic mellitus patients (DM group) had a higher prevalence of B. hominis infection compared to the non-DM group. …”
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Sagittal Craniosynostosis with Uncommon Anatomical Pathologies in a 56-Year-Old Male Cadaver by Andrey Frolov, Craig Lawson, Joshua Olatunde, James T. Goodrich, John R. Martin III

“…The histological analysis of the latter revealed atypical, noncompensatory massive bone overproduction. Exome sequencing of DNA extracted from the cadaveric tissue specimen performed on the Next Generation Sequencing (NGS) platform yielded 81 genetic variants identified as pathologic. …”
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Sanger validation of WGS variants by Arina Kopernik, Mariia Sayganova, Gaukhar Zobkova, Natalia Doroschuk, Anna Smirnova, Daria Molodtsova-Zolotukhina, Olesya Sagaydak, Oxana Ryzhkova, Sergey Kutsev, Olga Groznova, Lyusya Melikyan, Elizaveta Bondarchuk, Mary Woroncow, Eugene Albert, Viktor Bogdanov, Pavel Volchkov

“…Abstract With the development of next-generation sequencing (NGS) technologies it became possible to simultaneously analyze millions of variants. …”
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Usefulness of Pulsed-Field Gel Electrophoresis in Tracking Two Outbreaks of Invasive Meningococcal Disease Serogroup C in British Columbia by Grahame Quan, Mark Gilbert, Samara T David, Tazim Rahim, Kathy Adie, Carol Shaw, Alan McNabb, Judy Isaac-Renton, David M Patrick

“…Pulsed-field gel electrophoresis (PFGE) and porA gene sequencing of all retained IMD-C isolates were used to assess correlations between genotypes and epidemiological patterns. …”
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Antibiotic Resistance and Virulence Factors in Clinical Isolates of Stenotrophomonas maltophilia from Hospitalized Patients in Tehran, Iran by Mahrokh Bahrami, Narjess Bostanghadiri, Mehdi Goudarzi, Niloufar Khodaei, Ali Hashemi

“…This study conducted an analysis of antibiotic susceptibility by disc diffusion method and E-test assay, resistance and virulence gene frequencies were examined by PCR-sequencing, and multilocus sequencing typing (MLST) was performed for strain typing. …”
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