Isolation and characterization of mercuric reductase by newly isolated halophilic bacterium, Bacillus firmus MN8 by M. Noroozi, M.A. Amoozegar, A.A. Pourbabaei, N.S. Naghavi, Z. Nourmohammadi

“…Moreover, tracking and sequencing merA gene and kinetic properties of mercuric reductase in the selected strain were performed in this study. …”
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DeepRetention: A Deep Learning Approach for Intron Retention Detection by Zhenpeng Wu, Jiantao Zheng, Jiashu Liu, Cuixiang Lin, Hong-Dong Li

“…These metrics are not able to describe the pattern of sequencing depth of intronic reads, which is an intuitive and informative characteristic of retained introns. …”
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A Novel Pathogenic Splicing Mutation of OFD1 is Responsible for a Boy with Joubert Syndrome Exhibiting Orofaciodigital Spectrum Anomalies, Polydactyly and Retinitis Pigmentosa by Chen L, Zhao MF, Deng HW, Liao M, Fan LL, Zhong QB, Wang J, Li K, Wu ZH, Yin JY

“…Here, we identified a child who presented with Joubert syndrome exhibiting orofaciodigital spectrum anomalies, polydactyly, and retinitis pigmentosa. Whole exome sequencing and Sanger sequencing revealed a splicing mutation (NM_003611.2, c.2387+1G>A) in the OFD1 gene of the patient and his mother. mRNA sequencing further confirmed this mutation. …”
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A case report of confirmed difficult pulmonary tuberculosis based on the hybrid capture-based tNGS method by Weiqian Chen, Huimin Chen, Ze Liu, Xinle Chi, Yaomeng Chen, Huan Ye, Wenjie Huang, Chenlei Cao, Wei Weng

“…Currently, with the increasing popularity, iteration, and decreasing costs of Next-generation sequencing (NGS) testing technology, NGS is being more widely applied in the diagnosis of pulmonary tuberculosis. …”
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Metatranscriptomics as a tool to identify fungal species and subspecies in mixed communities – a proof of concept under laboratory conditions by Vanesa R. Marcelino, Laszlo Irinyi, John-Sebastian Eden, Wieland Meyer, Edward C. Holmes, Tania C. Sorrell

“…Abstract High-throughput sequencing (HTS) enables the generation of large amounts of genome sequence data at a reasonable cost. …”
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Endosperm culture-based allotriploid hybrid production from an interspecific cross of Haemanthus spp.: new insights into polyploidization and hybridization by Arisa Nakano, Masahiro Mii, Yoichiro Hoshino

“…In addition, chloroplast DNA sequencing revealed maternal inheritance in the endosperm-derived plantlets, consistent with embryo-derived plantlets. …”
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Comparisons of performances of structural variants detection algorithms in solitary or combination strategy. by De-Min Duan, Chinyi Cheng, Yu-Shu Huang, An-Ko Chung, Pin-Xuan Chen, Yu-An Chen, Jacob Shujui Hsu, Pei-Lung Chen

“…However, the precise identification and characterization of SVs remain challenging. While long-read sequencing offers superior accuracy for SV detection, short-read sequencing remains essential due to practical and cost considerations, as well as the need to analyze existing short-read datasets. …”
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Identification of the Hub Genes Involved in Stem Cell Treatment for Intervertebral Disc Degeneration: A Conjoint Analysis of Single-Cell and Machine Learning by Jianfeng Chen, Fuwei Qi, Guanshen Li, Qiaosong Deng, Chenlin Zhang, Xiaojun Li, Yafeng Zhang

“…In addition, the results of single-cell sequencing demonstrated the critical function of stem cells in IDD. …”
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Emerging technologies of single-cell multi-omics by Yi June Kim, Koichi Takahashi

“… The heterogeneity of the hematopoietic system was largely veiled by traditional bulk sequencing methods, which measure the averaged signals from mixed cellular populations. …”
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Isolation, Characterization, and Identification Candidate of Probiotic Bacteria Isolated from Wadi Papuyu (Anabas testudineus Bloch.) a Fermented Fish Product from Central Kalimant... by Yulistia Budianti Soemarie, Tiana Milanda, Melisa Intan Barliana

“…Identification results using the VITEK 2 Compact method and 16S rRNA gene PCR-sequencing showed that of the 4 isolated strains, three were bacterial and one belonged to yeasts.…”
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scRNA‐seq data analysis method to improve analysis performance by Junru Lu, Yuqi Sheng, Weiheng Qian, Min Pan, Xiangwei Zhao, Qinyu Ge

“…Abstract With the development of single‐cell RNA sequencing technology (scRNA‐seq), we have the ability to study biological questions at the level of the individual cell transcriptome. …”
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Dynamic Phase Signal Control Method for Unstable Asymmetric Traffic Flow at Intersections by Xiancai Jiang, Yu Jin, Yanli Ma

“…This paper addresses the limitations that the phases proposed in variable phase sequencing studies for stochastic traffic flow are all predetermined and that the variable phase sequencing is only suitable for low traffic volume environment. …”
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Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids by Lu Zhu, Wenhao Yang, Jiaxin Luo, Danli Lu, Yanan Hu, Rui Zhang, Yan Li, Li Qiu, Zelian Chen, Lina Chen, Hanmin Liu

“…RNA sequencing analysis of differentially expressed genes indicated a significant reduction in the immune response in NO. …”
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Silencing of STEAP3 suppresses cervical cancer cell proliferation and migration via JAK/STAT3 signaling pathway by Zouyu Zhao, Panpan Yu, Yan Wang, Hong Li, Hui Qiao, Chongfeng Sun, Lina Zhu, Ping Yang

“…The Reduced Representation Bisulfite Sequencing (RRBS) was used to detect global gene DNA methylation in CC tissues and paracancerous tissues. …”
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Preliminary exploration of mRNA, lncRNA, and miRNA expressions in the bovine jejunum unveils novel aspects of Mycobacterium avium subspecies paratuberculosis infections by Yahui Gao, Jie Cao, Bo Han, Dongxiao Sun

“…Results We applied whole transcriptome sequencing analysis to examine the jejunum tissue in nine Holstein cows. …”
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Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats by Tao-tao Lv, Yan-jun Mo, Tian-yuan Yu, Shuai Shao, Meng-qian Lu, Yu-ting Luo, Yi Shen, Yu-mo Zhang, Wong Steven

“…PCR results were consistent with those of RNA sequencing, suggesting that the results of transcriptome sequencing were reliable. …”
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Slight thermal stress exerts genetic diversity selection at coral (Acropora digitifera) larval stages by Cristiana Manullang, Nozomi Hanahara, Ariyo Imanuel Tarigan, Yuko Abe, Mao Furukawa, Masaya Morita

“…Many larval protein-coding sequences were identified in this genomic region, and the codon evolution of many of these genes showed signs of positive selection. …”
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Massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS) by Zhi Weng, Jiangxue Li, Yi Wu, Xuehao Xiu, Fei Wang, Xiaolei Zuo, Ping Song, Chunhai Fan

“…Nevertheless, unbiased information retrieval from low-copy-number sequences remains a barricade that largely arises from the indispensable DNA amplification. …”
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Exercise-induced methylation of the Serhl2 promoter and implication for lipid metabolism in rat skeletal muscle by Mutsumi Katayama, Kazuhiro Nomura, Jonathan M. Mudry, Alexander V. Chibalin, Anna Krook, Juleen R. Zierath

“…To determine the skeletal muscle DNA methylation signature of endurance swim training, we used whole-genome methylated DNA immunoprecipitation (MeDIP) sequencing. Methods: We utilized endurance-trained rats, cultured L6 myotubes, and human skeletal muscle cells, employing MeDIP sequencing, gene silencing, and palmitate oxidation assays. …”
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Identification of prognostic biomarkers of sepsis and construction of ceRNA regulatory networks by Guihong Chen, Wen Zhang, Chenglin Wang, Muhu Chen, Yingchun Hu, Zheng Wang

“…Subsequently, GO analysis was performed on the differential expression genes; Meta-analysis was used to screen for prognostic related genes; 10 × Single-cell RNA sequencing was used to annotate the cell distribution of core genes. …”
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