Genomic characterization of noroviruses from an outbreak associated with oysters by Annika Flint, Jennifer Harlow, Madison McLeod, Madeleine Blondin-Brosseau, Kelly Weedmark, Neda Nasheri

“…We next proceeded with whole-genome sequencing and obtained full genomes for 19 samples. …”
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RNF43 Mutations in IPMN Cases: A Potential Prognostic Factor by Xiao Yan Chang, Yan Wu, Ying Jiang, Peng Yan Wang, Jie Chen

“…The mutation rates of codons 12, 13, and 61 in KRAS and codon 201 in GNAS were detected by Sanger sequencing. Next-generation sequencing was performed on RNF43, and the results were further verified by Sanger sequencing. …”
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Development and validation of a minimal SNP genotyping panel for the differentiation of Cannabis sativa cultivars by Alex Cull, David L. Joly

“…Results In this study, we have conducted whole genome sequencing of 32 cultivars, mined the sequencing data for SNPs, developed a reduced SNP genotyping panel to discriminate between sequenced cultivars, then validated the 20-SNP panel using DNA from the sequenced cultivars and tested the assays on commercially available dried flower. …”
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Exploring the utility of snRNA-seq in profiling human bladder tissue: A comprehensive comparison with scRNA-seq by Briana Santo, Emily E. Fink, Alexandra E. Krylova, Yi-Chia Lin, Mohamed Eltemamy, Alvin Wee, Oliver Wessely, Byron H. Lee, Angela H. Ting

“…Summary: Single cell sequencing technologies have revolutionized our understanding of biology by mapping cell diversity and gene expression in healthy and diseased tissues. …”
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Screening for Janus kinase-2 exon 12 mutations among Janus kinase-2 V617F-negative polycythemia vera suspected Iraqi patients by Mushtaq M. Khazeem, Alaa Fadhil Alwan

“…Out of them, there were 102 patients with JAK2V617F-negative who retrospectively screened for JAK2 exon 12 mutations by direct sequencing (Sanger sequencing). RESULTS: Initial evaluation for the total 323 patients suspected with PV revealed that 84 cases were mutated for the JAK2V617F gene, and 239 were unmutated. …”
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Optimized genotyping method for identification of bacterial contaminants in pharmaceutical industry by Stamatoski Borche, Ilievska Miroslava, Babunovska Hristina, Sekulovski Nikola, Panov Sasho

“…Molecular identification of bacterial contaminants based on DNA sequencing of the hypervariable 16SrRNA gene has been introduced recently. …”
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Genomic Analysis Methods of Microorganisms by Canan Kebabçıoğlu, Osman Erganiş

“…MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. …”
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A Robotic Brain Scheme: Proposal Originated by a Modular Robotic Control by J. Negrete-Martínez

“…Finally, the planning and sequencing modules are imperative implementations in a robotic brain.…”
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Chromosome-scale genome assembly of Korean goosegrass (Eleusine indica) by Solji Lee, Changsoo Kim

“…We then combined Pore-C sequencing technology to successfully anchor 255 contigs to nine pseudochromosomes. …”
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How data science and AI-based technologies impact genomics by Jing Lin, Kee Yuan Ngiam

“…However, the accumulation of genomic data from sequencing and clinical data from electronic health records (EHRs) poses significant challenges for data scientists. …”
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Evaluation of various RNA-seq approaches for identification of gene outrons in the flatworm Opisthorchis felineus by N. I. Ershov, D. E. Maslov, N. P. Bondar

“…In the current study, we tested various experimental approaches for identifying the sequences of outrons in O. felineus using massive parallel sequencing. …”
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Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model by Hadi M. Alsakee, Hussein M. Abdulla, Reshna K. Albarzanji

“…Minor sequence polymorphisms, 1.67%, are observed in one of the isolates, whereas remarkable DNA sequence polymorphisms are noticed in three of the isolates. …”
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RETRACTED ARTICLE: Single-cell omics and machine learning integration to develop a polyamine metabolism-based risk score model in breast cancer patients by Xiliang Zhang, Hanjie Guo, Xiaolong Li, Wei Tao, Xiaoqing Ma, Yuxing Zhang, Weidong Xiao

“…Methods We used a multi-omics approach combining bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) to study polyamine metabolism. …”
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A scalable tool for analyzing genomic variants of humans using knowledge graphs and graph machine learning by Shivika Prasanna, Ajay Kumar, Deepthi Rao, Eduardo J. Simoes, Praveen Rao

“…Advances in high-throughput genome sequencing have enabled large-scale genome sequencing in clinical practice and research studies. …”
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Assembly-free reads accurate identification (AFRAID) approach outperforms other methods of DNA barcoding in the walnut family (Juglandaceae) by Yanlei Liu, Kai Chen, Lihu Wang, Xinqiang Yu, Chao Xu, Zhili Suo, Shiliang Zhou, Shuo Shi, Wenpan Dong

“…Species identification of 100% was achieved when chloroplast genome sequence coverage reached 20% and the original sequencing data reached 500,000 reads. …”
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The quest for environmental analytical microbiology: absolute quantitative microbiome using cellular internal standards by Chunxiao Wang, Yu Yang, Xiaoqing Xu, Dou Wang, Xianghui Shi, Lei Liu, Yu Deng, Liguan Li, Tong Zhang

“…However, the relative abundances derived from sequencing impede comparisons across samples and studies. …”
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Mitochondrial trifunctional protein deficiency caused by a deep intronic deletion leading to aberrant splicing by Thomas Cassini, Sarah Silverstein, Molly Behan, Cynthia J. Tifft, May Christine Malicdan, David R. Adams, Undiagnosed Diseases Network, Sun‐Young Ahn, Debra S. Regier

“…Both siblings were also diagnosed with focal segmental glomerulosclerosis (FSGS) and bone marrow failure and ultimately died of hypoxemic respiratory failure. Initial sequencing of the TFP‐associated genes HADHA and HADHB showed only a paternally inherited variant in HADHB, NM_000183.3:c.1059del (p.Gly354AspfsTer10). …”
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Distinct relative abundances in pathogens detected in mechanically ventilated patients with suspected pneumonia in the intensive care unit at King Abdulaziz University Hospital by Haifa Algethamy, Diyaa H. Bokhary, Ibrahim Abushoshah, Abdulrahman A. Alalyani, Maan K. Baamer, Dalya M. Attallah, Ruba M. Alotaibi, Shehla N. Amin, Shaza A. Mass, Nisreen R. Tashkandy

“…DNA from 83 deep endotracheal aspirate lung samples was subjected to PacBio sequencing to identify pathogens in comparison with conventional diagnostic techniques. …”
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Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum by FENG Chaoqun, RUAN Kunfei, SHI Shaotong, LI Sen, CHENG Shaoying, LIU Zhonghua

“…[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis in P . grandiflorum roots through transcriptome sequencing. [Methods] We performed transcriptome sequencing using Illumina high throughput sequencing technology. …”
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Genetic Characteristics of the Rat Fibroblast Cell Line Rat-1 by Thomas Liehr, Stefanie Kankel, Eva Miriam Buhl, Sarah K. Schröder-Lange, Ralf Weiskirchen

“…Additionally, we analyzed the transcriptome of Rat-1 cells through mRNA sequencing (mRNA-Seq) using next-generation sequencing (NGS). …”
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