Correlation analysis of DLG5 and PD-L1 expression in triple-negative breast cancer by Jingmin Che, Bo Chen, Xusheng Wang, Baoe Liu, Cuixiang Xu, Huxia Wang, Jingying Sun, Qing Feng, Xiangrong Zhao, Zhangjun Song

“…Prior research has indicated that the expression level of the cell polarity protein discs large homolog 5 (DLG5) correlates with the malignant progression and prognosis of breast cancer; nevertheless, its influence on PD-L1 expression and its function in immunotherapy for TNBC require further investigation. …”
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High-fat diet promotes gestational diabetes mellitus through modulating gut microbiota and bile acid metabolism by Lei Yao, Xuefei Zhou, Xianqi Jiang, Hao Chen, Yuanliang Li, Xiao Xiong, Yan Tang, Haogang Zhang, Pengfei Qiao

“…Expression levels of Fxr, Shp and Fgf15 mRNA and protein were detected by quantitative reverse transcription polymerase chain reaction and western blot, respectively.ResultsOur data indicated that high fat diet (HFD) was linked with higher prevalence of GDM, and HFD was positively associated with poor prognosis in GDM patients. …”
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FKBP5 as a key regulator of metabolic processes in birds: Insights from chicken pectoral muscle by Pengfei Du, Xiangli Zhang, Yao Zhu, Ziyang Wang, Xuemeng Si, Huaiyong Zhang, Yanqun Huang, Wen Chen

“…FK506-binding protein 5 (FKBP5) is a negative regulator of the glucocorticoid response and may play an important role in regulating metabolic homeostasis in birds. …”
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Comparison of the mid-infrared spectra and prediction equations developed from morning and evening milk samples from twice-a-day milked dairy cows by M. Frizzarin, E. Hayes, A. Casa, D.P. Berry

“…ABSTRACT: Mid-infrared spectroscopy is a technology used globally for quantifying the concentration of fat, protein, lactose, and other constituents in the milk samples of both individual animals and bulk tank milk. …”
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Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model by Tala Curry-Koski, Tala Curry-Koski, Liam P. Curtin, Mitra Esfandiarei, Mitra Esfandiarei, Mitra Esfandiarei, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas

“…Despite these significant clinical observations, investigation into cerebrovascular dysfunction and neuropathology in MFS remains limited.MethodsUsing 6M-control (C57BL/6) and 6M-MFS (Fbn1C1041G/+) and healthy 12M-control male and female mice, we test the hypothesis that abnormal Fbn1 protein expression is associated with altered cerebral microvascular density, BBB permeability, and neuroinflammation in the PCA-perfused hippocampus, all indicative of a pre-mature aging brain phenotype. …”
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Proximal, Microbiological and Nutritional Characterization in Chinese Potato Flour of the White Variety Colocasia Esculenta for Application in Functional Foods by F. López, L. Arboleda, V. González

“…By obtaining the flour, the proximal percentages are evaluated; moisture 9.01%, ash 2.24 ± 0.15% protein 2.02 ± 0.02% fiber 4.25 ± 0.26% and fat 0.43 ± 0.15%. …”
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Effects of Hypoxia Stress on Biochemical Indices and Expression of Hypoxia-Related Genes in the Heart of Rainbow Trout (Oncorhynchus mykiss) by Jiuju MA, Jinqiang HUANG, Yongjuan LI, Shenji WU, Lu ZHAO, Yue ZENG

“…Under severe hypoxic stress, lactate dehydrogenase gene (ldh), pyruvate kinase gene (pk), sdh, hif-1α, proline hydroxylase domain protein 2 (egln-1), and von Hippel-Lindau (vhl) expressions were significantly increased at 24 h (P < 0.05) compared to those under moderate hypoxic stress. …”
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Effectiveness and safety of PD-1/L1 inhibitors as first-line therapy for patients with advanced or metastatic urothelial carcinoma who are ineligible for platinum-based chemotherap... by Weiming Liang, Weiming Liang, Zhijing Wang, Zhilong Huang, Yanping Huang, Chunyan Li, Yiwen Liang, Miaoyan Huang, Duo Zhang, Chenchen Li

“…ObjectiveTo evaluate the efficacy and safety of programmed cell death protein 1 or its ligand (PD-1/L1) inhibitors as first-line therapy in advanced or metastatic urothelial carcinoma (mUC) who are ineligible for platinum-based chemotherapy.MethodA systematic search was conducted in four databases (Pubmed, Embase, Web of Science, and the Cochrane Library) to find articles that evaluate the effectiveness of first-line PD-1/L1 inhibitors for mUC, from the establishment of the databases to 22 November 2023. …”
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Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format by Kalvaityte U, Bagdonas E, Kirdaite G, Kausaite-Minkstimiene A, Uzieliene I, Ramanaviciene A, Popov A, Butkiene G, Karabanovas V, Denkovskij J, Mobasheri A, Bernotiene E

“…The purpose of the present research was to develop sensitive multiplex detection model of QD-based ELISA (QLISA), through the spectroscopic QD-analyte complex measurements in microvolume liquid droplets on a glass microslide.Methods: QLISA was used for the detection of cartilage oligomeric matrix protein (COMP) and human growth hormone (hGH) as model analytes. …”
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Development and validation of a hyperlipidemia risk prediction model for middle-aged and older adult Chinese using 2015 CHARLS data by Li-xiang Zhang, Shan-Bing Hou, Fang-fang Zhao, Ting-ting Wang, Ying Jiang, Xiao-juan Zhou, Jiao-yu Cao

“…The LASSO regression and multivariate Logistic regression analysis all revealed that Body Mass Index (BMI), fasting blood glucose, serum uric acid, C-reactive protein, and white blood cell count were independent risk factors for hyperlipidemia in this demographic (with Odds Ratios (OR) greater than 1 and p-values less than 0.05). …”
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Obtaining patient phenotypes in SARS-CoV-2 pneumonia, and their association with clinical severity and mortality by Fernando García-García, Dae-Jin Lee, Mónica Nieves-Ermecheo, Olaia Bronte, Pedro Pablo España, José María Quintana, Rosario Menéndez, Antoni Torres, Luis Alberto Ruiz Iturriaga, Isabel Urrutia, COVID-19 & Air Pollution Working Group

“…Our clustering algorithm identified k=3 distinct phenotypes and 18 strongly informative variables: Phenotype A (788 cases [50.9% prevalence] – age $$\sim$$ ∼ 57, Charlson comorbidity $$\sim$$ ∼ 1, pneumonia CURB-65 score $$\sim$$ ∼ 0 to 1, respiratory rate at admission $$\sim$$ ∼ 18 min-1, FiO2 $$\sim$$ ∼ 21%, C-reactive protein CRP $$\sim$$ ∼ 49.5 mg/dL [median within cluster]); phenotype B (620 cases [40.0%] – age $$\sim$$ ∼ 75, Charlson $$\sim$$ ∼ 5, CURB-65 $$\sim$$ ∼ 1 to 2, respiration $$\sim$$ ∼ 20 min-1, FiO2 $$\sim$$ ∼ 21%, CRP $$\sim$$ ∼ 101.5 mg/dL); and phenotype C (140 cases [9.0%] – age $$\sim$$ ∼ 71, Charlson $$\sim$$ ∼ 4, CURB-65 $$\sim$$ ∼ 0 to 2, respiration $$\sim$$ ∼ 30 min-1, FiO2 $$\sim$$ ∼ 38%, CRP $$\sim$$ ∼ 152.3 mg/dL). …”
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Early Postoperative Safety of Total Hip Arthroplasty in Systemic Lupus Erythematosus Patients by YANG Xingdong, YU Muyang, XU Yiming, ZHU Wei, HU Mingwei, WENG Xisheng, FENG Bin

“…After propensity score matching, 163 cases from SLE and control groups were included for analysis. (1) Regarding medical complications, compared with control group, SLE group showed significant differences in osteoporosis, respiratory system disorders, gastrointestinal diseases, urinary system disorders, hematologic abnormalities, and secondary or concomitant rheumatic diseases (all P < 0.05). (2) In terms of preoperative laboratory tests, SLE group had lower platelet counts, absolute lymphocyte counts, hemoglobin levels, hematocrit, albumin levels, blood glucose levels, and activated partial thromboplastin times than control group, while C-reactive protein, erythrocyte sedimentation rate, and D-dimer levels were higher (all P < 0.05). (3) Regarding surgical-related indicators, a higher proportion of patients in SLE group had an ASA grade > 2 (15.95% vs. 3.07%, P < 0.001), but intraoperative blood loss was significantly smaller [324.7 (200.0, 500.0) mL vs. 421.8 (200.0, 500.0) mL, P=0.005]. (4) In terms of complications, SLE group exhibited a significantly higher incidence of major complications than control group (8.59% vs. 1.23%, P=0.005), with a relative risk of 1.081 (95% CI: 1.028-1.136). …”
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Serum ALT activity and its isoenzymes as potential biomarkers for diagnosis of Sarcopenia in older adults: a retrospective, cross-sectional study by Jiangping Zeng, Nannan Li, Jiaying Ge, Huihui Ma, Siqi Sun, Yujie Jing, Chunhua Qian, Ran Cui, Shen Qu, Hui Sheng

“…Abstract Background Alanine aminotransferase (ALT) is an enzyme crucial for energy and protein metabolism in muscle cells. Despite this, its association with sarcopenia remains inadequately explored. …”
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Identification of Lauric Acid as a Potent Sodium Channel NaV1.5 Blocker from Compound Chinese Medicine Wenxin Keli by Xie W, Gao J, Liang Y, Huang C, Zhang B, Chen X, Yao X, Nan G, Wu H, Wang Y, Wu L, Wang T, Zhu Y

“…Molecular docking and molecular dynamics simulation suggested that LA binds to the NaV 1.5 protein, with a significant binding affinity forming interactions with functionally essential residues and blocks the inward flow of Na+. …”
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Cucumeropsis mannii seed oil shields against cyclophosphamide-induced hepatorenal toxicity by modulating redox imbalance and iNOS/IL-β1 signalling in rats by Amaka Cecilia Agbara, Ejike Daniel Eze, Christian Emeka Offor, Adam Moyosore Afodun, Ezebuilo Ugbala Ekpono, Peter Chinedu Agu, Chinyere Aloke, Nkeiru Nwaamaka Ezeani, Emmanuel Orire Ikuomola, Ekom Monday Etukudo, Ilemobayo Victor Fasogbon, Angela Mumbua Musyoka, Patrick Maduabuchi Aja

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Nucleated red blood cells for characterization of systemic inflammatory response syndrome in dogs by ARom Cho, Hyeona Bae, Youngju Kim, Yeseul Jeon, Rankyung Jung, Minji Kim, Minjeong Kang, Sumin Cha, Kyu‐Woan Cho, Dong‐In Jung, Jinho Park, DoHyeon Yu

“…Results The median WBC (17.63, interquartile range [IQR]: 11.72‐20.24 × 109/L), neutrophils (12.28, IQR: 7.17‐16.88 × 109/L), band neutrophils (1288.5, IQR: 252.5‐2575 cells/μL), serum IL‐6 (731.80, IQR: 299.79‐5522.05 pg/mL), and plasma C‐reactive protein (4.10, IQR: 1.00‐8.58 mg/L) were significantly higher in nRBC‐positive dogs than negative dogs (11.27, IQR: 7.63‐15.13 × 109/L; 7.57, IQR: 4.96‐11.71 × 109/L; 62.5, IQR: 0‐350.25 cells/μL; 232.30, IQR: 99.33‐447.01 pg/mL; 0.40, IQR: 0.10‐3.00 mg/L, respectively; P < .05). …”
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A c-type lectin HcLec1 with dual function of immunology and mineralization from the freshwater oyster (Hyriopsis cumingii Lea) by Xiaofeng Chen, Xiaoya Shen, Shijun Liu, Wenjuan Li, Wenjuan Li, He Wang, Jiale Li, Jiale Li, Zhiyi Bai, Zhiyi Bai

“…The recombinant HcLec1 protein demonstrated binding affinity to LPS and PGN, a robust ability to agglutinate Escherichia coli, Staphylococcus aureus, Aeromonas veronii, and Aeromonas hydrophila, and significantly inhibited bacterial growth (P &lt; 0.05). …”
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Biosynthesis and activity of Zn-MnO nanocomposite in vitro with molecular docking studies against multidrug resistance bacteria and inflammatory activators by Samy Selim, Tarek M. Abdelghany, Mohammed S. Almuhayawi, Mohammed K. Nagshabandi, Muyassar K. Tarabulsi, Mohammed Yagoub Mohammed Elamir, Asmaa A. Alharbi, Soad K. Al Jaouni

“…MnO-NPs and ZnO-NPs demonstrated inhibitory activity against the crystal structure of putative minohydrolase (PDB ID: 4EWT), methicillin acyl-penicillin binding protein 2a structure (PDB ID: 1MWU) and K2U bound crystal structure of class II peptide deformylase from MRSA (PDB ID: 6JFQ). …”
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Long-term trends and patterns in ultra-processed food consumption among Korean adults from 1998 to 2022 by Hyeseung Lee, Yesol Yim, Yerin Cho, Jiyeon Oh, Soeun Kim, Yejun Son, Hyesu Jo, Seohyun Hong, Masoud Rahmati, Hanseul Cho, Jiyoung Hwang, Dong Keon Yon

“…In 2020–2022, ultra-processed foods had a higher proportion of calories from carbohydrates (62.12% vs. 58.81%) and fats (28.22% vs. 22.31%) but a lower proportion from protein (10.32% vs. 17.26%) compared to non-ultra-processed foods. …”
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Elevated D-dimer on admission may predict poor prognosis in childhood influenza associated encephalopathy by Huizhen Wang, Lingkong Zeng, Xingfeng Cheng, Hui Li, Hong Zhang, Yuanmei Shi, Yong Zhang, Changjian Li

“…In the univariate analysis, the poor prognosis group compared with the clinically cured group showed a significant increase in the number of days of hospitalization (3.0 vs. 9.5 days, P < 0.001), high-sensitivity C-reactive protein (6.80 vs. 1.88 mg/L, P = 0.003), interleukin-6 (20.26 vs. 8.24 pg/mL, P = 0.001), interleukin-10 (11.75 vs. 4.72 pg/mL, P = 0.003), alanine aminotransferase (104.0 vs. 20.0 U/L, P = 0.011), aspartate azelotransferase (186.5 vs. 37.0 U/L, P = 0.003), serum albumin (37.99 vs. 40.76 g/L, P = 0.042), prothrombin time (13.2 vs. 11.4 s, P = 0.017), D-dimer (4.34 vs. 0.44 mg/L FEU, P < 0.001), peripheral blood CD19 B-cell count (35.11 vs. 32.75 cells/µL, P = 0.018), and cerebrospinal fluid chloride (126.82 vs. 125.50 mmol/L, P = 0.027) were statistically different in the above 11 indicators. …”
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