HEALTH-PROMOTING PROPERTIES OF BROAD BEANS (VICIA FABA L.) by Alicja Baranowska

“…A review of the research literature concludes that broad bean seeds are a rich source of plant proteins, complex carbohydrates, essential vitamins, minerals and phytochemicals. …”
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Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70 by Marc A. Morizono, Kelly L. McGuire, Natalie I. Birouty, Mark A. Herzik

“…In human mitochondria, mitochondrial Hsp70 (mortalin) and the nucleotide exchange factor (GrpEL1) work synergistically to stabilize proteins, assemble protein complexes, and facilitate protein import. However, our understanding of the molecular mechanisms guiding these processes is hampered by limited structural information. …”
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High-resolution fleezers reveal duplex opening and stepwise assembly by an oligomer of the DEAD-box helicase Ded1p by Eric M. Patrick, Rajeev Yadav, Kasun Senanayake, Kyle Cotter, Andrea A. Putnam, Eckhard Jankowsky, Matthew J. Comstock

“…Abstract DEAD-box RNA-dependent ATPases are ubiquitous in all domains of life where they bind and remodel RNA and RNA-protein complexes. DEAD-box ATPases with helicase activity unwind RNA duplexes by local opening of helical regions without directional movement through the duplexes and some of these enzymes, including Ded1p from Saccharomyces cerevisiae, oligomerize to effectively unwind RNA duplexes. …”
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Structure and function of a near fully-activated intermediate GPCR-Gαβγ complex by Maxine Bi, Xudong Wang, Jinan Wang, Jun Xu, Wenkai Sun, Victor Ayo Adediwura, Yinglong Miao, Yifan Cheng, Libin Ye

“…Consequently, the functions of intermediate GPCR-G protein complexes remain elusive. Guided by a conformational landscape visualized via 19F quantitative NMR and molecular dynamics (MD) simulations, we determined the structure of an intermediate GPCR-mini-Gαsβγ complex at 2.6 Å using cryo-EM, by blocking its transition to the fully activated complex. …”
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Abscission zone metabolism impacts pre- and post-harvest fruit quality: a very attaching story by Timothy J. Tranbarger, Francisco R. Tadeo

“…We focus on studies that have provided new insight into possible protein complexes that function in the AZ, including multiple MADS-box transcription factors, with potential overlapping regulatory roles which exist between AZ development, ethylene production, AZ activation, fruit ripening and overall fruit quality. …”
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Computer-aided approaches reveal trihydroxychroman and pyrazolone derivatives as potential inhibitors of SARS-CoV-2 virus main protease by Atatreh Noor, Hasan Shaima, Ali Bassam R., Ghattas Mohammad A.

“…MD simulations of 300 ns for the ligand-protein complexes of SN02 and SN18 affirmed the previously attained results of the potential inhibition activity of these two ligands. …”
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TCTEX1D2 is essential for sperm flagellum formation in mice by Ryua Harima, Kenshiro Hara, Kentaro Tanemura

“…Abstract Flagella and cilia are widely conserved motile structures, in mammalian, sperm possess flagella. Large protein complexes called dynein, including cytoplasmic dynein 2 and axonemal dynein, play a role in the formation of cilia and flagella. …”
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Non-enzymatic protein targeting agents as a promising strategy for cancer treatment by Madison Ambrose, Jeremy Lee, Aleem Syed, Zamal Ahmed, Guang Peng

“…The second approach targets protein-protein interactions (PPIs) as an alternative non-enzymatic route that alters the functional activities of protein complexes and thus significantly influence cancer cell fitness and survival. …”
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The impact of terahertz radiation on an extremophilic archaean Halorubrum saccharovorum proteome by T. N. Goryachkovskaya, S. H. Konstantinova, I. A. Meshcheriakova, S. V. Bannikova, E. A. Demidov, A. V. Bryanskaya, M. A. Scheglov, A. I. Semenov, D. Yu. Oshchepkov, V. M. Popik, S. E. Peltek

“…The aim of this study was the identification of protein complexes participating in the response of the archaea Halorubrum saccharovorum H3 isolated from an extreme natural environment to terahertz radiation. …”
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Alpha synuclein and inflammaging by Geneviève L. Putnam, Robert W. Maitta

“…There is a growing amount of data regarding α-syn normal function in the body that includes involvement in cellular transport such as protein complexes assembly, vesicular trafficking, neurotransmitter release, as well as immune cell maturation. …”
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PPAR-γ Signaling Crosstalk in Mesenchymal Stem Cells by Ichiro Takada, Alexander P. Kouzmenko, Shigeaki Kato

“…Treatment with Wnt5a-activated NLK (nemo-like kinase) induced physical association of the endogenous NLK and H3K9 histone methyltransferase (SETDB1) protein complexes with PPAR-γ. This resulted in histoneH3K9 tri-methylation at PPAR-γ target gene promoters. …”
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Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes by Jue Lin, Joshua Cheon, Rashida Brown, Michael Coccia, Eli Puterman, Kirstin Aschbacher, Elizabeth Sinclair, Elissa Epel, Elizabeth H. Blackburn

“…Telomeres, the protective DNA-protein complexes at the ends of linear chromosomes, are important for genome stability. …”
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Loss of H2A.Z Is Not Sufficient to Determine Transcriptional Activity of Snf2-Related CBP Activator Protein or p400 Complexes by Tamara A. Bowman, Madeline M. Wong, Linda K. Cox, Joseph J. Baldassare, John C. Chrivia

“…The p400 and SRCAP (Snf2-related CBP activator protein) complexes remodel chromatin by catalyzing deposition of histone H2A.Z into nucleosomes. …”
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Structure of an ex vivoDrosophila TOM complex determined by single-particle cryoEM by Agalya Periasamy, Pamela Ornelas, Thomas Bausewein, Naomi Mitchell, Jiamin Zhao, Leonie M. Quinn, Werner Kuehlbrandt, Jacqueline M. Gulbis

“…As the first reported structure of a transgenic protein expressed and purified ex vivo from Drosophila, the method provides impetus for parallel structural and genetic analyses of protein complexes linked to human pathology. The core TOM complex extracted from native membranes of the D. melanogaster retina contains transgenic Tom40 co-assembled with four endogenous TOM components: Tom22, Tom5, Tom6 and Tom7. …”
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Metal-Enhanced Fluorescence of Chlorophylls in Light-Harvesting Complexes Coupled to Silver Nanowires by Dorota Kowalska, Bartosz Krajnik, Maria Olejnik, Magdalena Twardowska, Nikodem Czechowski, Eckhard Hofmann, Sebastian Mackowski

“…While fluorescence spectra are identical both in terms of overall shape and maximum wavelength for peridinin-chlorophyll-protein complexes both isolated and coupled to metallic nanostructures, we conclude that interaction with plasmon excitations in the latter remains neutral to the functionality of the biological system. …”
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Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitoch... by Haiqing Yu, Joann J. Lu, Wei Rao, Shaorong Liu

“…Density gradient centrifugation is widely utilized for various high purity sample preparations, and density gradient ultracentrifugation (DGU) is often used for more resolution-demanding purification of organelles and protein complexes. Accurately locating different isopycnic layers and precisely extracting solutions from these layers play a critical role in achieving high-resolution DGU separations. …”
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Protein Ubiquitylation in Pancreatic Cancer by Thomas Bonacci, Julie Roignot, Philippe Soubeyran

“…Indeed, despite its role in promoting protein degradation through the proteasome, ubiquitylation is now known to regulate diverse cellular processes, such as membrane protein endocytosis and intracellular trafficking, assembly of protein complexes, gene transcription, and activation or inactivation of enzymes. …”
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Behavioral phenotyping of Kaiso-deficient mice by V. S. Korostina, A. V. Kulikov

“…It binds methylated DNA with its zinc-finger domain and recruits repressive protein complexes to the methylated DNA fragments by the interaction of the BTB/POZ domain with the complex of NCoR corepressor and histone deacetylase, thereby performing transcription repression. …”
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CUL4-Based Ubiquitin Ligases in Chromatin Regulation: An Evolutionary Perspective by Makiko Nakagawa, Tadashi Nakagawa

“…It is orchestrated by the concerted action of three types of enzymes, with substrate specificity governed by ubiquitin ligases (E3s), which may exist as single proteins or as part of multi-protein complexes. Although Cullin (CUL) proteins lack intrinsic enzymatic activity, they participate in the formation of active ubiquitin ligase complexes, known as Cullin-Ring ubiquitin Ligases (CRLs), through their association with ROC1 or ROC2, along with substrate adaptor and receptor proteins. …”
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Plant expression systems for production of recombinant pharmaceutically important proteins by E. V. Deineko, A. A. Zagorskaya

“…In addition, plants are higher eukaryotes, and therefore fullvalue folding and the formation of multimeric protein complexes occur in their cells, as well as a significant portion of post­translational modifications similar to those in mammalian cells. …”
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