Protocol for the purification of the plastid-encoded RNA polymerase from transplastomic tobacco plants by Xiao-Xian Wu, Fan Li, Chuxia Zhu, Shu-Yi Sun, Wen-Hui Mu, Stephanie Ruf, Ralph Bock, Yu Zhang, Fei Zhou

“…Here, we present a strategy to purify the transcriptionally active protein complex from transplastomic tobacco (Nicotiana tabacum) lines in which one of the PEP core subunits is fused to an epitope tag. …”
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EMC2 suppresses ferroptosis via regulating TFRC in nasopharyngeal carcinoma by Xianghui Chen, Xiaoyan Wang, Yuxia Zou, Yan Wang, Tingting Duan, Zijie Zhou, Yi Huang, Qing Ye

“…The levels of endoplasmic reticulum membrane protein complex 2 (EMC2) in NPC tissue microarrays were evaluated by immunohistochemistry, and the prognostic value of EMC2 was analyzed in NPC patients. …”
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GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…By RNA-sequencing and experiments in vitro using multiple ovarian cancer cell models as well as in vivo using subcutaneous xenografts assay and in situ transplantation ovarian cancer model in nude mice, we further demonstrated that RAB8A positively mediated OC progression through activating HH signaling pathway by disassociating the protein–protein complex formation of GLI and SuFu (Suppressor of Fused), which reciprocally enhanced GPR137 activity, forming a regulation loop between HH signaling and GPR137. …”
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A dual role of Cohesin in DNA DSB repair by Michael Fedkenheuer, Yafang Shang, Seolkyoung Jung, Kevin Fedkenheuer, Solji Park, Davide Mazza, Robin Sebastian, Hiroyuki Nagashima, Dali Zong, Hua Tan, Sushil Kumar Jaiswal, Haiqing Fu, Anthony Cruz, Supriya V. Vartak, Jan Wisniewski, Vittorio Sartorelli, John J. O’Shea, Laura Elnitski, Andre Nussenzweig, Mirit I. Aladjem, Fei-Long Meng, Rafael Casellas

“…Cohesin, a multi-subunit protein complex, plays a crucial role in both chromosome organization and DNA repair by creating architectural loops through chromatin extrusion. …”
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SMARCA4 deficiency in small cell lung cancer: A case report and narrative review of the literature by Andreas M. Matthaiou, Ioannis Tomos, Nikoleta Bizymi, Ioannis Vamvakaris, Nektarios Anagnostopoulos, Aikaterini Papadopoulou, Kalliopi Angelou, Grigoris Stratakos, Adamantia Liapikou

“… SWItch/Sucrose Non-Fermentable (SWI/SNF) is a large protein complex with a central role in chromatin remodeling and genome transcription. …”
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The endocytic adaptor AP-2 maintains Purkinje cell function by balancing cerebellar parallel and climbing fiber synapses by Marianna Tolve, Janine Tutas, Ebru Özer-Yildiz, Ines Klein, Anne Petzold, Veronika J. Fritz, Melina Overhoff, Quinn Silverman, Ellie Koletsou, Filip Liebsch, Guenter Schwarz, Tatiana Korotkova, Silvana Valtcheva, Graziana Gatto, Natalia L. Kononenko

“…We show that endocytic adaptor protein complex 2 (AP-2) is crucial for Purkinje cell survival. …”
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Kuiyangling Enema Alleviates Ulcerative Colitis Mice by Reducing Levels of Intestinal NETs and Promoting HuR/VDR Signaling by Zhang D, Zhu Z, He Z, Duan S, Yi Q, Qiu M, Dai X, Su G, Li K, Xu L, Liu D, Wu Y, Gao Y, Li R, Guo S

“…Immunohistochemistry and immunofluorescence analyses were conducted to assess the levels of HuR/VDR protein complex, ZO-1, Occludin, Claudin-7, and intestinal NETs. …”
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Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice by Chi Lieu Kim Nguyen, Yumeno Kuba, Hoai Thu Le, Hossam Hassan Shawki, Natsuki Mikami, Madoka Aoki, Nanako Yasuhara, Hayate Suzuki, Saori Mizuno-Iijima, Shinya Ayabe, Yuki Osawa, Tomoyuki Fujiyama, Tra Thi Huong Dinh, Miyuki Ishida, Yoko Daitoku, Yoko Tanimoto, Kazuya Murata, Woojin Kang, Masatsugu Ema, Yuji Hirao, Atsuo Ogura, Satoru Takahashi, Fumihiro Sugiyama, Seiya Mizuno

“…Our study reveals that the exocyst complex, a heterotetrameric protein complex important for tethering in vesicular transport, is important for proper intra-oocyte trafficking of c-KIT and GDF9 in mice. …”
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Structural basis of RNA polymerase complexes in African swine fever virus by Guoliang Zhu, Fei Xi, Wuxia Zeng, Yifei Zhao, Weijun Cao, Chen Liu, Fan Yang, Yi Ru, Shuqi Xiao, Shilei Zhang, Huanan Liu, Hong Tian, Fayu Yang, Biao Lu, Shukai Sun, Haiyang Song, Bozhang Sun, Xiaoyi Zhao, Lijie Tang, Kangli Li, Jijun He, Jianhong Guo, Yun Zhu, Zixiang Zhu, Fei Sun, Haixue Zheng

“…The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and regulation. …”
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MagIC beads for scarce macromolecules by Carlos Moreno-Yruela, Beat Fierz

“…Specialized magnetic beads that bind target proteins to a cryogenic electron microscopy grid make it possible to study the structure of protein complexes from dilute samples.…”
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Comparison of Trace Elements in High-Molecular-Mass Multiprotein Complex and in Female Milk from Which It Was Obtained by Svetlana E. Soboleva, Natalia P. Zaksas, Georgy A. Nevinsky

“…Such metal ions can be important for the formation and biological function of protein complexes.…”
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Calcium bridges built by mitochondria-associated endoplasmic reticulum membranes: potential targets for neural repair in neurological diseases by Yichen Peng, Li Zhou, Yaju Jin, Danli Wu, Na Chen, Chengcai Zhang, Hongpeng Liu, Chunlan Li, Rong Ning, Xichen Yang, Qiuyue Mao, Jiaxin Liu, Pengyue Zhang

“…Certain proteins or protein complexes within these membranes directly or indirectly regulate the distance between the endoplasmic reticulum and mitochondria, as well as the transduction of Ca2+ signaling. …”
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Drosophila Torsin Protein Regulates Motor Control and Stress Sensitivity and Forms a Complex with Fragile-X Mental Retardation Protein by Phuong Nguyen, Jong Bok Seo, Hyo-Min Ahn, Young Ho Koh

“…In addition, we also found that human Torsin1A and human FMRP were present in the same protein complexes, suggesting that this phenomenon is evolutionarily conserved.…”
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Inflammasomes in Mycobacterium tuberculosis-Driven Immunity by Sebastian Wawrocki, Magdalena Druszczynska

“…The key role in this process is played by inflammasomes, multimeric protein complexes serving as a platform for caspase-1, an enzyme responsible for proteolytic cleavage of IL-1β and IL-18 precursors. …”
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An integrated approach using proximity labelling and chemical crosslinking to probe in situ host-virus protein–protein interactions by Jiaqi Li, Zhewang Lin

“…Traditional proteomic efforts based on affinity purification of protein complexes from cell lysates are limited to detecting strong and stable interactions. …”
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RNA Editing and Drug Discovery for Cancer Therapy by Wei-Hsuan Huang, Chao-Neng Tseng, Jen-Yang Tang, Cheng-Hong Yang, Shih-Shin Liang, Hsueh-Wei Chang

“…RNA editing is vital to provide the RNA and protein complexity to regulate the gene expression. Correct RNA editing maintains the cell function and organism development. …”
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Alternative Splicing: Emerging Roles in Anti-Aging Strategies by Lingyue Gao, Rong Jia

“…Alternative splicing plays a fundamental role in gene expression and protein complexity. Aberrant splicing impairs cell homeostasis and is closely associated with aging and cellular senescence. …”
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Mechanistic studies on the effect of endogenous proteins on the starch retrogradation characteristics of corn before and after postharvest ripening by Nannan Hu, Weihua Qi, Jinying Zhu, Fuyin Zhao, Lin Xiu, Mingzhu Zheng, Jingsheng Liu

“…The corn starch-protein complexes before postharvest ripening (JD-0) and after postharvest ripening (JD-40) were subjected to protease treatment, and the influence of protein on starch retrogradation was studied. …”
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Structural basis of human VANGL-PRICKLE interaction by Yanyi Song, Shuyi Jian, Junlin Teng, Pengli Zheng, Zhe Zhang

“…The well-organized polarity pattern in cells is established by the asymmetric distribution of two core protein complexes on opposite sides of the cell membrane. …”
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A Pharmacological and Toxicological Profile of Silver as an Antimicrobial Agent in Medical Devices by Alan B. G. Lansdown

“…The present paper discusses the mechanisms of absorption and metabolism of silver in the human body, presumed mechanisms of argyria and argyrosis, and the elimination of silver-protein complexes in the bile and urine. Minimum blood silver levels consistent with early signs of argyria or argyrosis are not known. …”
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