Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations by Nele Niebrügge, Olga Trovato, Roman Praschberger, Andreas Lieb

“…For a more comprehensive investigation of heterotrimeric G-protein complex signaling, we investigated the impact of WT mutants in combination with (i) a specifically suggested assay, and (ii) the most abundantly expressed heterotrimeric G-protein complex combinations in WT receptor-enriched regions. …”
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Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis by Shuai Zheng, Lise C. Noack, Ouda Khammy, Andreas De Meyer, Ghazanfar Abbas Khan, Nancy De Winne, Dominique Eeckhout, Daniël Van Damme, Staffan Persson

“…To further demonstrate the power of PUP-IT, we used the system to identify protein interactions of the protein complex that underpin cellulose synthesis in plants. …”
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Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma by David A. Rowbotham, Katey S. S. Enfield, Victor D. Martinez, Kelsie L. Thu, Emily A. Vucic, Greg L. Stewart, Kevin L. Bennewith, Wan L. Lam

“…Our results indicate that VHL elongin BC protein complex components other than VHL could be important for PCC tumorigenesis and merit further investigation.…”
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Illuminating the impact of N-terminal acetylation: from protein to physiology by Nina McTiernan, Ine Kjosås, Thomas Arnesen

“…We also provide an overview of the impact of N-terminal acetylation, including its effects on protein folding, subcellular targeting, protein complex formation, and protein turnover. In particular, there may be competition between N-terminal acetyltransferases and other enzymes in defining protein fate. …”
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Recent advances in plant kinetochore research by Elena Kozgunova, Elena Kozgunova

“…Faithful chromosome segregation is crucial for cell division in eukaryotes, facilitated by the kinetochore, a multi-subunit protein complex that connects chromosomes to the spindle microtubules. …”
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Pulmonary surfactant system by Zafer Durgun, Ercan Keskin

“…Pulmonary surfactant, a phospholipid-protein complex that lines the alveoli and small airways of the lungs, is secreted by type ll alveolar epithelial cells. …”
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Heat Shock Protein 70 Neutralizes Apoptosis-Inducing Factor by Guido Kroemer

“…For example, cytochrome c, which is normally confined to the mitochondrial intermembrane space, is liberated from mitochondria and interacts with a cytosolic protein, Apaf-1, causing its oligomerization and constitution of the so-called apoptosome, a protein complex which activates a specific class of cysteine proteases, the caspases[2]. …”
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Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei by Lei Li, May G. Akl, Scott B. Widenmaier

“…We present a protocol to isolate fixed DNA-protein complexes from mouse liver prior to chromatin shearing. …”
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T Cell Costimulatory Molecules in Anti-Viral Immunity: Potential Role in Immunotherapeutic Vaccines by Tania H Watts, Edward M Bertram, Jacob Bukczynski, Tao Wen

“…The activation of T cells requires both an antigen specific signal, involving the recognition of a peptide/major histocompatibility protein complex by the T cell receptor, as well as additional costimulatory signals. …”
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Molecular Imaging of Hypoxia-Inducible Factor 1α and von Hippel-Lindau Interaction in Mice by Clara Y.H. Choi, Denise A. Chan, Ramasamy Paulmurugan, Patrick D. Sutphin, Quynh-Thu Le, Albert C. Koong, Wayne Zundel, Sanjiv S. Gambhir, Amato J. Giaccia

“…Under normoxia, HIF-1α is hydroxylated on prolines 402 and 564 and is targeted for ubiquitin-mediated degradation by interacting with the von Hippel-Lindau protein complex (pVHL). We have developed a novel method of studying the interaction between HIF-1α and pVHL using the split firefly luciferase complementation-based bioluminescence system in which HIF-1α and pVHL are fused to amino-terminal and carboxy-terminal fragments of the luciferase, respectively. …”
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Pulmonary fibrosis as the sole manifestation of anti-Ku antibody positivity in the absence of myositis: A case report by Angelo Nigro, Dr.

“…Background: Anti-Ku antibodies are autoantibodies directed against the Ku protein complex involved in DNA repair. They are typically associated with overlap syndromes featuring polymyositis and systemic sclerosis. …”
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Loss of Notch dimerization perturbs intestinal homeostasis by a mechanism involving HDAC activity. by Quanhui Dai, Kristina Preusse, Danni Yu, Rhett A Kovall, Konrad Thorner, Xinhua Lin, Raphael Kopan

“…A tri-protein complex containing NICD, RBPj and MAML1 binds DNA as monomer or as cooperative dimers to regulate transcription. …”
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Finding the Correct Partner: The Meiotic Courtship by Tomás Naranjo

“…In the worm Caenorhabditis elegans, each chromosome carries a pairing center consisting of a chromosome-specific DNA-protein complex, and in the fission yeast Schizosaccharomyces pombe, the sme2 locus encodes a meiosis-specific non-coding RNA that mediates on homologous recognition. …”
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A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins by K. A. Mooslehner, J. D. Davies, I. A. Hughes

“…Of those, 47 were common to both samples suggesting different AR protein complex components in proliferating and proliferation-inhibited cells from the mouse proximal caput epididymus. …”
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Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin by Ruoshi Xu, Qiumei Kang, Jie Ren, Zukun Li, Xiaoping Xu

“…The results infer that the multimeric protein complex of β-catenin could be increased by CGA upregulated genes GSK-3β and APC, which could inhibit the free β-catenin into the nucleus to connect with TCF. …”
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The Drosophila homologue of the amyloid precursor protein is a conserved modulator of Wnt PCP signaling. by Alessia Soldano, Zeynep Okray, Pavlina Janovska, Kateřina Tmejová, Elodie Reynaud, Annelies Claeys, Jiekun Yan, Zeynep Kalender Atak, Bart De Strooper, Jean-Maurice Dura, Vítězslav Bryja, Bassem A Hassan

“…Molecularly, both human APP and fly APPL form complexes with PCP receptors, thus suggesting that APPs are part of the membrane protein complex upstream of PCP signaling. Moreover, we show that APPL regulates PCP pathway activation by modulating the phosphorylation of the Wnt adaptor protein Dishevelled (Dsh) by Abelson kinase (Abl). …”
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Structural Exploration and Conformational Transitions in MDM2 upon DHFR Interaction from Homo sapiens: A Computational Outlook for Malignancy via Epigenetic Disruption by Arundhati Banerjee, Sujay Ray

“…Residual disclosure from the best suited simulated protein complex disclosed 18 side chain and 3 ionic interactions to strongly accommodate MDM2 protein into the pocket-like zone in DHFR due to the positive environment by charged residues. …”
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Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters by Syed Zawar Shah, Thomas N. Perry, Andrea Graziadei, Valentina Cecatiello, Thangavelu Kaliyappan, Agata D. Misiaszek, Christoph W. Müller, Ewan P. Ramsay, Alessandro Vannini

“…At U6 snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc) and a Brf2-containing TFIIIB complex, forming a pre-initiation complex (PIC). …”
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Identification of Binding Partners of Deafness-Related Protein PDZD7 by Haibo Du, Rui Ren, Panpan Chen, Zhigang Xu, Yanfei Wang

“…PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV1, whirlin, harmonin, SANS, and MYO7A, all belonging to USH proteins. …”
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Preparation and application of glucono-δ-lactone-induced gel of transglutaminase cross-linked black bean protein isolate-whey protein isolate: Effect of ultrasound pretreatment by Yuexin Liu, Yichen Zhang, Fengjuan Dong, Qingkui Zhao, Shuang Zhang, Chen Tan

“…These findings will guide the design of double protein complex gels, providing possible avenues for use as carriers of biologically active substances such as riboflavin.…”
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