New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells by Xiangjun He, Yin-Xiong Li, Bo Feng

“…Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). …”
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Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Zhihan Zhao, Huan Lan, Ibrahim El-Battrawy, Xin Li, Fanis Buljubasic, Katherine Sattler, Gökhan Yücel, Siegfried Lang, Malte Tiburcy, Wolfram-Hubertus Zimmermann, Lukas Cyganek, Jochen Utikal, Thomas Wieland, Martin Borggrefe, Xiao-Bo Zhou, Ibrahim Akin

“…Background. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery. …”
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Optimized Method to Generate Well-Characterized Macrophages from Induced Pluripotent Stem Cells by Qimin Hai, Peter Bazeley, Juying Han, Gregory Brubaker, Jennifer Powers, Claudia M. Diaz-Montero, Jonathan D. Smith

“…The objectives of this study are to define a robust and consistent method for the differentiation of induced pluripotent stem cells (iPSCs) into macrophages that can address these needs for recurrent studies with high yields and the potential for gene editing. …”
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Modeling Alzheimer’s Disease with Induced Pluripotent Stem Cells: Current Challenges and Future Concerns by Weiwei Zhang, Bin Jiao, Miaojin Zhou, Tao Zhou, Lu Shen

“…We will then highlight the existing induced pluripotent stem cell models of AD and, lastly, discuss the potential clinical applications in this field.…”
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Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells by Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko

“…Human pluripotent stem cells (hPSCs) have the capacity to differentiate into essentially all cell types in the body. …”
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Generating Diverse Spinal Motor Neuron Subtypes from Human Pluripotent Stem Cells by Rickie Patani

“…The ability to predictably manipulate MN subtype lineage restriction from human pluripotent stem cells (PSCs) will form the essential basis to establishing accurate, clinically relevant in vitro disease models. …”
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Induced pluripotent stem cell–related approaches to generate dopaminergic neurons for Parkinson’s disease by Ling-Xiao Yi, Hui Ren Woon, Genevieve Saw, Li Zeng, Eng King Tan, Zhi Dong Zhou

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Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers by Junjun Li, Itsunari Minami, Leqian Yu, Kiyotaka Tsuji, Minako Nakajima, Jing Qiao, Masato Suzuki, Ken Shimono, Norio Nakatsuji, Hitetoshi Kotera, Li Liu, Yong Chen

“…Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) hold high potential for use in drug assessment and myocardial regeneration. …”
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Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair by Shinji Kotaka, Shigeyuki Wakitani, Akira Shimamoto, Naosuke Kamei, Mikiya Sawa, Nobuo Adachi, Mituo Ochi

“…We consider labeled induced pluripotent stem (iPS) cells to be an ideal source of cells for tissue regeneration, and if iPS cells could be delivered only into cartilage defects, it would be possible to repair articular cartilage. …”
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Small Molecules Greatly Improve Conversion of Human-Induced Pluripotent Stem Cells to the Neuronal Lineage by Sally K. Mak, Y. Anne Huang, Shifteh Iranmanesh, Malini Vangipuram, Ramya Sundararajan, Loan Nguyen, J. William Langston, Birgitt Schüle

“…Here, we describe our neurodifferentiation protocol using small molecules that very efficiently promote neural induction in a 5-stage EB protocol from six induced pluripotent stem cells (iPSC) lines from patients with Parkinson’s disease and controls. …”
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Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of Mouse-Induced Pluripotent Stem Cells by Yan Huang, Xiaofang Chen, Jifei Che, Qi Zhan, Jing Ji, Yubo Fan

“…In this study, we focused on the impact of different magnitudes of shear stress on the differentiation of mouse-induced pluripotent stem cells (iPSCs) towards arterial or venous ECs. …”
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Differences in the Gene Expression Profiles of Slow- and Fast-Forming Preinduced Pluripotent Stem Cell Colonies by Sujin Kwon, Jung Sun Park, Byungkuk Min, Yong-Kook Kang

“…Induced pluripotent stem cells (iPSCs) are generated through a gradual process in which somatic cells undergo a number of stochastic events. …”
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Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells by Huan Yi, Bingbing Xie, Ben Liu, Xuan Wang, Li Xu, Jia Liu, Min Li, Xiufeng Zhong, Fuhua Peng

“…Induced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development. …”
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Prospect of Human Pluripotent Stem Cell-Derived Neural Crest Stem Cells in Clinical Application by Qian Zhu, Qiqi Lu, Rong Gao, Tong Cao

“…In this review, we first discuss NCSC generation methods from human pluripotent stem cells and differentiation mechanism of NCSCs. …”
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Derivation of two induced pluripotent stem cell lines from a healthy control subject by Dan Zhang, Di Huang, Leon M. Larcher, Khine Zaw, Shang-Chih Chen, Luke Jennings, Tina M. Lamey, Jennifer A. Thompson, Terri L. McLaren, Fred K. Chen, Samuel McLenachan

“…Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. …”
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CRISPR/Cas9-Based Protocol for Precise Genome Editing in Induced Pluripotent Stem Cells by Avinash Singh, Swathy Babu, Marcus Phan, Shauna Yuan

“…However, the editing of induced pluripotent stem cells (iPSCs) with CRISPR presents notable challenges in ensuring cell survival and achieving high editing efficiency. …”
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Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm by Ines Ross Tacco, Joseph Olshausen, Tse Yuan Chan, Naima Turbes, Ming-Yow Hung, Chi-Tai Yeh, Patricia K. Nguyen, Karim Sallam, Nazish Sayed, Ian Y. Chen

“…These lines demonstrated normal morphology and karyotypes, robust expression of pluripotency markers, and ability for tri-lineage differentiation. …”
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Patient-derived induced pluripotent stem cell organoids for amyotrophic lateral sclerosis drug discovery by Wenyan Li, Jingqi Liu, Wenting Li, Lina Sun, Hao Zhang, Lei Gao, Chong Gao

“…Patient-derived induced pluripotent stem cell (iPSC) organoids have emerged as an innovative tool for disease modeling and drug evaluation. …”
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Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells by Sherry Li Zheng, Jonas L. Fowler, Julie Y. Chen, Christopher Li, Elaine Lin, Alana T. Nguyen, Angela Chen, George Q. Daley, Lay Teng Ang, Kyle M. Loh

“…Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated, seeded, and then sequentially differentiated into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and hematopoietic progenitors through the sequential addition of defined, serum-free media. …”
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The search for microRNAs potentially involved in the selfrenewal maintaining of laboratory rat pluripotent stem cells by V. V. Sherstyuk, S. P. Medvedev, M. T. Ri, Y. V. Vyatkin, O. V. Saik, D. N. Shtokalo, S. M. Zakian

Subjects: “…pluripotency…”
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