Exploring aneuploidies in two-center isolated bovine embryonic stem cell lines: Implications for cultured meat production by Chloë Deelkens, Elly De Vlieghere, Mario Van Poucke, Masaki Kinoshita, Jeffrey Aalders, Lieven Thorrez, Bert Devriendt, Ann Van Soom, Luc Peelman, Björn Menten, Catharina De Schauwer, Jolanda van Hengel

“…These cells maintained core pluripotency markers (POU5F1, SOX2, SALL4, NANOG) and demonstrated the ability to differentiate into the three germ layers, indicating their potential for sustainable food applications. …”
Get full text

Migration, Proliferation, and Differentiation of Cord Blood Mesenchymal Stromal Cells Treated with Histone Deacetylase Inhibitor Valproic Acid by Leah A. Marquez-Curtis, Yuanyuan Qiu, April Xu, Anna Janowska-Wieczorek

“…We found that in MSC, VPA increased (i) the gene and total protein expression of CXCR4 and CXCR7 and primed migration towards a low gradient of SDF-1, (ii) the gene expression of MMP-2 and secretion and activation of proMMP-2, (iii) the proliferation and gene expression of pluripotency markers SOX2 and Oct-4, and exposure to lower concentrations of VPA (≤5 mM) had no effect on their differentiation to osteocytes and chondrocytes. …”
Get full text

TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6 by Sheng Luo, Wenjin Wang, Jingfang Feng, Rui Li

“…As a novel core part of pluripotency circuitry, testicular expression 10 (TEX10) plays an actively noticeable effect on reprogramming, early embryo development, and embryonic stem cell self-renewal. …”
Get full text

Regulation of meristem and hormone function revealed through analysis of directly-regulated SHOOT MERISTEMLESS target genes by Tamara Lechon, Nicholas A. Kent, James A. H. Murray, Simon Scofield

“…It regulates the expression of target genes that include transcriptional regulators associated with meristem function, particularly those involved in pluripotency and cellular differentiation, as well as genes involved in hormone metabolism and signaling. …”
Get full text

Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis by Shiki Fujino, Norikatsu Miyoshi

“…The Oct4 gene plays an important role in undifferentiated embryonic stem cells and regulates stem cell pluripotency. The aim of this study was to examine the relationship between Oct4 expression and liver metastasis of colorectal cancer (CRC) in clinical samples and investigate the role and abilities of Oct4-positive CRC cells. …”
Get full text

Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms by Anna Labedz-Maslowska, Elzbieta Kamycka, Sylwia Bobis-Wozowicz, Zbigniew Madeja, Ewa K. Zuba-Surma

“…Thus, we employed multi-instrumental flow cytometric approach including classical and imaging cytometry and we established that newly identified population of nonhematopoietic cells expressing CD106 (VCAM-I) antigen contains SCs with very small size, expressing markers of pluripotency (Oct-4A and Nanog) on both mRNA and protein levels that indicate VSEL population. …”
Get full text

Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells by Yuzhou Gu, Yuzhou Gu, Yuzhou Gu, Kexin Wu, Kexin Wu, Kexin Wu, Bowen Niu, Bowen Niu, Bowen Niu, Zhiting Wang, Zhiting Wang, Zhiting Wang, Yuchen Jie, Yuchen Jie, Yuchen Jie, Zixuan Fan, Zixuan Fan, Zixuan Fan, Junying Li, Junying Li, Congjiao Sun, Congjiao Sun, Congjiao Sun, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Li-Wa Shao, Li-Wa Shao, Li-Wa Shao

“…Cell viability, glycogen granule contents, and mRNA expression levels of pluripotency markers (NANOG and POUV), germ cell-specific markers (DAZL and CVH), and telomerase reverse transcriptase (TERT) were reduced in PGCs cultured under high temperatures (43°C for 12, 24, and 48 h). …”
Get full text

Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore by Zhenkui Qin, Aichang Ji, Meng Yan, Danwen Liu, Xixi Li, Xiaoli Hu, Zhifeng Zhang

“…Immunofluorescence detection of KLF4, c-MYC, MYOSIN, and 5-HT indicated that CfT cells may possess a certain degree of pluripotency. Four gene transfer methods were tested in the CfT cell line; however, the transfer efficiency (0.5 %-1 %) requires further optimization. …”
Get full text

Comparison of the MicroRNA Expression Profiles of Male and Female Avian Primordial Germ Cell Lines by Bence Lázár, Mahek Anand, Roland Tóth, Eszter Patakiné Várkonyi, Krisztina Liptói, Elen Gócza

“…As microRNAs have been proved to play a key role in the maintenance of pluripotency and the cell cycle regulation of stem cells, we continued with a complex miRNA analysis. …”
Get full text

A method for siRNA-mediated knockdown of target genes in RA-induced neurogenesis using P19 cells by Hossein Khodadadi, Hiroaki Taniguchi

“…Validation of the differentiation was performed through quantitative reverse transcription PCR (RT-qPCR) analysis, measuring the expression levels of key neuronal markers, including Map2 and Pax6 along with the pluripotency marker Oct4. Additionally, the efficiency of the siRNA-mediated knockdown was confirmed by western blot analysis, which demonstrated significant gene silencing at protein levels. …”
Get full text

Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice by Chao Chen, Dina Zhu, Shuai Zhang, Wensheng Zhang

“…The expanded network of proteins interacting with these 5 genes shows that the TGF-β signaling pathway, signaling pathways regulating pluripotency of stem cell, fat digestion and absorption, and PPAR signaling pathway was highly enriched. …”
Get full text

H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts by Wilhelm Bouchereau, Hong-Thu Pham, Worawalan Samruan, Van-Hong Vu, Thierry Joly, Marielle Afanassieff, Pierre Savatier, Rangsun Parnpai, Nathalie Beaujean

“…These modifications impact the expression of genes associated with pluripotency and lineage determination, underscoring the importance of H3K9 modifications in embryonic cell fate decisions.…”
Get full text

The role of VANGL2 in glioma oncogenesis and progression: insights into expression profiles and prognostic relevance by Mingyu Zhao, Mingyu Zhao, Mingyu Zhao, Mingyu Zhao, Shanshuang Chen, Shanshuang Chen, Shanshuang Chen, Shanshuang Chen

“…Functional enrichment analysis of genes positively associated with VANGL2 in glioma underscored their enrichment in Notch signaling and pathway regulating pluripotency of stem cells. In vitro experiments further confirmed that VANGL2 promotes glioma cell migration, invasion, proliferation, and tumor sphere formation. …”
Get full text

STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation by Yu Qian Wong, Hongyan Xu, Qiang Wu, Xinyu Liu, Chengchen Lufei, Xiu Qin Xu, Xin-Yuan Fu

“…Taken together, the findings of this study indicated that InSTAT3 KO and InSTAT3 CA ESCs could provide a new tool to clarify the direct targets of STAT3 and its role in ESC maintenance, which will facilitate the elaboration of the mechanisms whereby STAT3 maintains ESC pluripotency and regulates ESC differentiation during mammalian embryogenesis.…”
Get full text

Irisin reshapes bone metabolic homeostasis to delay age-related osteoporosis by regulating the multipotent differentiation of BMSCs via Wnt pathway by Shangman Xing, Yifan Ma, Bing Song, Bing Song, Min Bai, Kexin Wang, Wenjing Song, Tingting Cao, Chao Guo, Yanying Zhang, Zhandong Wang, Yongfeng Wang, Yongfeng Wang

“…Simultaneously, the Wnt signaling pathway might be a crucial mechanism through which irisin sustains the bone-fat balance in aging BMSCs and mitigates the decline in pluripotency. In vivo, irisin reduced bone marrow fat deposition in aging mice and effectively alleviating the occurrence of bone loss.ConclusionIrisin mediates the Wnt signaling pathway, thereby influencing the fate determination of BMSCs. …”
Get full text

Long noncoding RNAs predict the survival of patients with colorectal cancer as revealed by constructing an endogenous RNA network using bioinformation analysis by Hui Zhang, Zhuo Wang, Jianzhong Wu, Rong Ma, Jifeng Feng

“…Functional enrichment analysis suggested that the target genes of the ceRNA network may be involved in the pathways related to cancer, including the signaling pathway that regulates the pluripotency of stem cells, wnt signaling pathway, hippo signaling pathway, basal cell carcinoma, and colorectal cancer. …”
Get full text

Comprehensive analysis of inhibin-β A as a potential biomarker for gastrointestinal tract cancers through bioinformatics approaches by Rohit Kumar Verma, Prashant Kumar Srivastava, Ashutosh Singh

“…The KEGG pathway analysis revealed that INHBA and its interacting proteins are involved in several pathways, including TGF-beta signaling, Signalling pathways regulating pluripotency of stem cells, colorectal cancer, pancreatic cancer, AGE-RAGE signaling, and so on as major pathways. …”
Get full text

Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis by Jane Carr-Wilkinson, Nilendran Prathalingam, Deepali Pal, Mohammad Moad, Natalie Lee, Aishwarya Sundaresh, Helen Forgham, Peter James, Mary Herbert, Majlinda Lako, Deborah A. Tweddle

“…Expression of the pluripotency marker OCT 4 decreased, whereas TP53 and LIN28B expression remained high at levels similar to SHSY5Y and IMR32 neuroblastoma cell lines. …”
Get full text

The potential role of differentially expressed tRNA-derived fragments in high glucose-induced podocytes by Zhenxing Zhang, Yunyang Qiao, Jialing Ji, Chan Huang, Huimin Shi, Weihua Gan, Aiqing Zhang

“…KEGG pathway analysis revealed that tRFs may be involved in signaling pathways related to growth hormones, phospholipase D, the regulation of stem cell pluripotency, and T-/B-cell receptors. Overexpression of tRF-1:24, one of the most differentially expressed tRFs, attenuated podocyte injury induced by HG. …”
Get full text

Computer analysis of co-localization of transcription factor binding sites in genome by ChIP-seq data by A. I. Dergilev, A. M. Spitsina, I. V. Chadaeva, A. V. Svichkarev, F. M. Naumenko, E. V. Kulakova, E. R. Galieva, E. E. Vityaev, M. Chen, Y. L. Orlov

“…Analysis of new ChIPseq data about binding of transcription factors Nr5a2, Tbx3 in the same cell type has confirmed the division of clusters of transcription factors binding sites into two types: those containing the binding sites of regulators of pluripotency (Oct4, Nanog, and others) and those not. …”
Get full text