Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation by Sergio Mora-Castilla, Juan R. Tejedo, Rafael Tapia-Limonchi, Irene Díaz, Ana B. Hitos, Gladys M. Cahuana, Abdelkrim Hmadcha, Franz Martín, Bernat Soria, Francisco J. Bedoya

“…The function of pluripotency genes in differentiation is a matter of investigation. …”
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Alternative Splicing in Self-Renewal of Embryonic Stem Cells by Clara Y. Cheong, Thomas Lufkin

“…Much of embryonic stem cell biology has focused on transcriptional expression and regulation of genes that could mediate its unique potential in self-renewal or pluripotency. In alignment with our present understanding on the genetic, protein, and epigenetic factors that may direct cell fate, we present a short overview of the often overlooked contribution of alternative splice variants to regulatory diversity. …”
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Open problems in synthetic multicellularity by Ricard Solé, Núria Conde–Pueyo, Jordi Pla–Mauri, Jordi Garcia–Ojalvo, Nuria Montserrat, Michael Levin

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Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure by S. Arnhold, S. Glüer, K. Hartmann, O. Raabe, K. Addicks, S. Wenisch, M. Hoopmann

“…In order to show stem cell characteristics such as pluripotency and to evaluate a possible therapeutic application of these cells, AF fluid-derived stem cells were differentiated along the adipogenic, osteogenic, and chondrogenic as well as the neuronal lineage under hypoxic conditions. …”
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Stemness-Attenuating miR-503-3p as a Paracrine Factor to Regulate Growth of Cancer Stem Cells by Minkoo Seo, Seung Min Kim, Eun Young Woo, Ki-Cheol Han, Eun Joo Park, Seongyeol Ko, Eun wook Choi, Mihue Jang

“…The expression of pluripotency genes was significantly decreased in miR-503-3p-treated CSCs. …”
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Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential by Kristi A. Hohenstein Elliott, Cory Peterson, Anuradha Soundararajan, Natalia Kan, Brandon Nelson, Sean Spiering, Mark Mercola, Gary R. Bright

“…Laser-mediated propagation maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential. …”
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Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate by Lijuan Pei, Hongjie Zhang, Meihui Zhang, Yixuan Wang, Ke Wei

“…Rcor2, the corepressor 2 of REST, a transcriptional repressor, is predominantly expressed in embryonic stem cells (ESCs) and plays a major role in regulating ESC pluripotency and neurogenesis. The function of Rcor2 in development of other germ layers is yet unclear. …”
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Embryonic Stem Cell-Derived Cardiomyocyte Heterogeneity and the Isolation of Immature and Committed Cells for Cardiac Remodeling and Regeneration by Kenneth R. Boheler, Robert N. Joodi, Hui Qiao, Ondrej Juhasz, Amanda L. Urick, Sandra L. Chuppa, Rebekah L. Gundry, Robert P. Wersto, Rong Zhou

“…Pluripotent stem cells represent one promising source for cell replacement therapy in heart, but differentiating embryonic stem cell-derived cardiomyocytes (ESC-CMs) are highly heterogeneous and show a variety of maturation states. …”
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Culture and Use of Mesenchymal Stromal Cells in Phase I and II Clinical Trials by Bourin Philippe, Sensebé Luc, Planat-Bénard Valérie, Roncalli Jérôme, Bura-Rivière Alessandra, Casteilla Louis

“…Their potential has been extended to pluripotency, by their possibility of differentiating into tissues and cells of nonmesodermic origin. …”
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Epigenetic Induction of Definitive and Pancreatic Endoderm Cell Fate in Human Fibroblasts by Rangarajan Sambathkumar, Eric Kalo, Rob Van Rossom, Marijke M. Faas, Paul de Vos, Catherine M. Verfaillie

“…Interestingly, TSA-CRM did not affect expression of pluripotency and hepatocyte genes but induced some mesoderm transcripts. …”
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An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro by Li Zhang, Yenan Wu, Xiang Li, Shao Wei, Yiming Xing, Zhengxing Lian, Hongbing Han

“…The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro.…”
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The Role of Epigenetic in Dental and Oral Regenerative Medicine by Different Types of Dental Stem Cells: A Comprehensive Overview by Ahmed Hussain, Hamid Tebyaniyan, Danial Khayatan

“…Several lines of evidence have shown that epigenetic modification of DNA and DNA-correlated histones are necessary for determining cells’ phenotypes and regulating stem cells’ pluripotency and renewal capacity. It is increasingly recognized that nuclear enzyme activities, such as histone deacetylases, can be used pharmacologically to induce stem cell differentiation and dedifferentiation. …”
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Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents by Foued Ghanjati, Simeon Santourlidis

“…Unrestricted somatic stem cells (USSCs) from human umbilical cord blood, which have a broad differentiation spectrum, reside in an uncommitted epigenetic state with partial methylation of the regulatory region of the gene coding for the pluripotency master regulator OCT4. Thus we hypothesized that further opening of this “poised” epigenetic state could broaden the differentiation potential of USSCs. …”
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Mesenchymal Stem/Stromal Cells from Discarded Neonatal Sternal Tissue: In Vitro Characterization and Angiogenic Properties by Shuyun Wang, Lakshmi Mundada, Eric Colomb, Richard G. Ohye, Ming-Sing Si

“…Neonatal sternal bone MSCs (sbMSCs) were clonogenic, had a surface marker expression profile that was characteristic of bone marrow MSCs, were multipotent, and expressed pluripotency-related genes at low levels. Neonatal sbMSCs also demonstrated in vitro proangiogenic properties. …”
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Enigma of Retrotransposon Biology in Mammalian Early Embryos and Embryonic Stem Cells by Ying Yin, Liquan Zhou, Shuiqiao Yuan

“…Additionally, endeavors made to unveil the roles of these mysterious elements may facilitate stem cell status conversion and manipulation of pluripotency.…”
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Collagen Modulates the Biological Characteristics of WJ-MSCs in Basal and Osteoinduced Conditions by Vun Vun Hiew, Peik Lin Teoh

“…Gene expression profiles showed that COL not only maintained the pluripotency, self-renewal, and immunophenotype of WJ-MSCs but also primed cells toward lineage differentiations by upregulating BMP2 and TGFB1 genes. …”
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Long-Term Cultured Human Term Placenta-Derived Mesenchymal Stem Cells of Maternal Origin Displays Plasticity by Vikram Sabapathy, Saranya Ravi, Vivi Srivastava, Alok Srivastava, Sanjay Kumar

“…Flow analysis established bonafied MSCs phenotypic characteristics, staining positively for CD29, CD73, CD90, CD105 and negatively for CD14, CD34, CD45 markers. Pluripotency of the cultured MSCs was assessed by in vitro differentiation towards not only intralineage cells like adipocytes, osteocytes, chondrocytes, and myotubules cells but also translineage differentiated towards pancreatic progenitor cells, neural cells, and retinal cells displaying plasticity. …”
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Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis by Jurate Savickiene, Grazina Treigyte, Sandra Baronaite, Giedre Valiuliene, Algirdas Kaupinis, Mindaugas Valius, Audrone Arlauskiene, Ruta Navakauskiene

“…Using two-stage protocol, MSCs were successfully cultured and exhibited typical stem cell morphological, specific cell surface, and pluripotency markers characteristics. AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. …”
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Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro by Divya S. Varghese, Shama Parween, Mustafa T. Ardah, Bright Starling Emerald, Suraiya A. Ansari

“…In this study, we found that antibiotics, pen-strep, and gentamicin did not affect hESC cell viability or expression of pluripotency markers. However, during directed differentiation towards neural and hepatic fate, significant cell death was noted through the activation of caspase cascade. …”
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The Impact of Bioactive Lipids on Cardiovascular Development by Alexander Kleger, Stefan Liebau, Qiong Lin, Götz von Wichert, Thomas Seufferlein

“…Interestingly, S1P may also act to maintain human stem cell pluripotency. Both LPA and S1P positively regulate the proliferative capacity of murine ES cells. …”
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