TAB2 deficiency induces dilated cardiomyopathy by promoting mitochondrial calcium overload in human iPSC-derived cardiomyocytes by Wenrui Sun, Jianchao Zhang, Shuang Li, Wanrong Fu, Yangyang Liu, Mengduan Liu, Jianzeng Dong, Xiaoyan Zhao, Xiaowei Li

“…Methods In this study, we generated a TAB2 compound heterozygous knockout cell line in induced pluripotent stem cells (iPSCs) derived from a healthy individual using CRISPR/Cas9 technology. …”
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Altered cytoskeleton dynamics in patient-derived iPSC-based model of PCDH19 clustering epilepsy by Rossella Borghi, Stefania Petrini, Valentina Apollonio, Marina Trivisano, Nicola Specchio, Sandra Moreno, Enrico Bertini, Marco Tartaglia, Claudia Compagnucci

“…Here, we evaluate the consequences of altered PCDH19 function on microfilaments and microtubules organization, using a disease model obtained from patient-derived induced pluripotent stem cells. We show that iPSC-derived cortical neurons are characterized by altered cytoskeletal dynamics, suggesting that this protocadherin has a role in modulating stability of MFs and MTs. …”
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Nanosensor-based imaging of realtime dopamine release in neurons derived from iPSCs of patients with Parkinson's disease by Nayeon Lee, Dakyeon Lee, Jae Hyeok Lee, Bo Seok Lee, Sungjee Kim, Jae Ho Kim, Sanghwa Jeong

“…In this study, we applied NIRCat to elucidate DA release in human induced pluripotent stem cells (hiPSCs)-derived dopaminergic neurons from both healthy control and PD patient carrying GBA1 mutations. …”
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Highly efficient construction of monkey blastoid capsules from aged somatic cells by Junmo Wu, Tianao Shao, Zengli Tang, Gaojing Liu, Zhuoyao Li, Yuxi Shi, Yu Kang, Jiawei Zuo, Bo Zhao, Guangyu Hu, Jiaqi Liu, Weizhi Ji, Lei Zhang, Yuyu Niu

“…In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. …”
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Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALB/c Mice by Thi Thao Do, Thi Phuong Do, Thi Nga Nguyen, Thi Cuc Nguyen, Thi Thu Phuong Vu, Thi Giang An Nguyen

“…The in vitro cytotoxic activity of liposomal L-ASP was less effective against LLC, MCF-7 (human breast carcinoma), HepG2 (human hepatocellular carcinoma), SK-LU-1 (human lung carcinoma), and NTERA-2 (pluripotent human embryonic carcinoma) cells than that of free L-ASP. …”
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Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture by Zhi-Gang Xue, Zhan-Ping Shi, Juan Dong, Ting-Ting Liao, Yan-Peng Wang, Xue-Ping Sun, Zheng-Jie Yan, Xiao-Qiao Qian, Yu-Gui Cui, Juan Chen, Jia-Yin Liu, Guoping Fan

“…Human primary fibroblasts are a popular type of somatic cells for the production of induced pluripotent stem (iPS) cells. Here we characterized biological properties of primary fibroblasts in terms of cell-growth rate, cytogenetic stability, and the number of inactive X chromosomes during long-term passaging. …”
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G6PD deficiency triggers dopamine loss and the initiation of Parkinson’s disease pathogenesis by Morgan G. Stykel, Shehani V. Siripala, Eric Soubeyrand, Carla L. Coackley, Ping Lu, Suelen Camargo, Sharanya Thevasenan, Gerardo Balderas Figueroa, Raphaella W.L. So, Erica Stuart, Rachi Panchal, Elissavet-Kalliopi Akrioti, Jeffery T. Joseph, Omid Haji-Ghassemi, Era Taoufik, Tariq A. Akhtar, Joel C. Watts, Scott D. Ryan

“…Using murine and human pluripotent stem cell (hPSC) models of PD coupled with human postmortem tissue, we show that accumulation of α-syn micro-aggregates impairs metabolic flux through the pentose phosphate pathway (PPP). …”
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Human Fetal Liver: An In Vitro Model of Erythropoiesis by Guillaume Pourcher, Christelle Mazurier, Yé Yong King, Marie-Catherine Giarratana, Ladan Kobari, Daniela Boehm, Luc Douay, Hélène Lapillonne

“…Human embryonic stem (ES) cells or induced pluripotent stem cell (iPS) are the natural candidates. …”
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…More recently, human intestinal organoids (HIOs), a new 3D in vitro model derived from pluripotent stem cells, have the advantage to closely resemble the architecture of the intestinal mucosa. …”
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HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant Dendritic Cells by Yoo-Wook Kwon, Hyo-Suk Ahn, Jin-Woo Lee, Han-Mo Yang, Hyun-Jai Cho, Seok Joong Kim, Shin-Hyae Lee, Heung-Mo Yang, Hyun-Duk Jang, Sung Joo Kim, Hyo-Soo Kim

“…Although human induced pluripotent stem cells (iPSCs) can serve as a universal cell source for regenerative medicine, the use of iPSCs in clinical applications is limited by prohibitive costs and prolonged generation time. …”
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Metabolic adaptations to acute glucose uptake inhibition converge upon mitochondrial respiration for leukemia cell survival by Monika Komza, Jesminara Khatun, Jesse D. Gelles, Andrew P. Trotta, Ioana Abraham-Enachescu, Juan Henao, Ahmed Elsaadi, Andriana G. Kotini, Cara Clementelli, JoAnn Arandela, Sebastian El Ghaity-Beckley, Agneesh Barua, Yiyang Chen, Mirela Berisa, Bridget K. Marcellino, Eirini P. Papapetrou, Masha V. Poyurovsky, Jerry Edward Chipuk

“…When wild-type and oncogenic RAS patient-derived induced pluripotent stem cell acute myeloid leukemia (AML) models were examined, KL-11743 mediated induction of mitochondrial respiration and dependency for survival associated with oncogenic RAS. …”
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Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“… POU5F1, a key transcription factor, plays a pivotal role in maintaining pluripotency and cellular differentiation, making it of immense interest in developmental biology and animal husbandry. …”
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Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog by Ting Ye, Jingyuan Li, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Jianyu Wang, H. Rosie Xing

“…The orphan nuclear receptor Nr5a2 is a transcriptional factor, and a regulator of stemness of embryonic stem cells and induced pluripotent stem cells. However, whether Nr5a2 regulates the self‐renewal of lung CSCs is unknown. …”
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Susceptibility to Ventricular Arrhythmias Resulting from Mutations in FKBP1B, PXDNL, and SCN9A Evaluated in hiPSC Cardiomyocytes by Hector Barajas-Martinez, Maya Smith, Dan Hu, Robert J. Goodrow, Colleen Puleo, Can Hasdemir, Charles Antzelevitch, Ryan Pfeiffer, Jacqueline A. Treat, Jonathan M. Cordeiro

“…We assessed the functional impact of these mutations in induced pluripotent stem cell cardiomyocytes (hiPSC-CMs) derived from the proband and an unaffected family member. …”
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The importance of stem cells in the treatment of type 1 diabetes, type 2 diabetes and diabetes complications by Monika Kamińska, Maria Jasiewicz, Magdalena Miernik-Skrzypczak, Aleksandra Spyra, Marta Malicka, Natalia Dąbrowska, Aleksandra Kozioł, Katarzyna Moczyróg

“…For type 1 diabetes, MSCs reduced inflammation, preserved pancreatic islet function, and decreased insulin requirements without major adverse effects. Pluripotent stem cells showed promise in islet transplantation, achieving stable glycemic control in clinical trials. …”
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Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment by Kenta Takeuchi, Shunsuke Tabe, Yuya Yamamoto, Kenta Takahashi, Megumi Matsuo, Yasuharu Ueno, Masayuki Ohtsuka, Soichiro Morinaga, Yohei Miyagi, Tomoyuki Yamaguchi, Naoki Tanimizu, Hideki Taniguchi

“…We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including heterogeneous cancer-associated fibroblasts (CAFs), using patient-derived PDAC cells and human-induced pluripotent cell-derived endothelial and mesenchymal cells. …”
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Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components by Malena Herrera Lopez, Matías Bertone Arolfo, Mónica Remedi, Laura Gastaldi, Carlos Wilson, Gonzalo G. Guendulain, Danilo Ceschin, Andrés Cardozo Gizzi, Alfredo Cáceres, Ana Lis Moyano

“…Human neural rosettes (hNRs) are radial cell structures that assemble from induced pluripotent stem cells (hiPSCs) and recapitulate some stages of neural tube morphogenesis. …”
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Four cardiomyopathy patients with a heterozygous DSG2 p.Arg119Ter variant by Takuya Sumida, Shou Ogawa, Shuichiro Higo, Yuki Kuramoto, Ryo Eto, Yoshihiko Ikeda, Congcong Sun, Junjun Li, Li Liu, Tomoka Tabata, Yoshihiro Asano, Mikio Shiba, Yasuhiro Akazawa, Daisuke Nakamura, Takafumi Oka, Tomohito Ohtani, Yasushi Sakata

“…A microforce test using human cardiomyocytes differentiated from induced pluripotent stem cells (iPSC-CMs) demonstrated reduced contractility in iPSC-CMs carrying a heterozygous truncating variant in DSG2. …”
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Distinct regulation of Tau Monomer and aggregate uptake and intracellular accumulation in human neurons by Amir T. Marvian, Tabea Strauss, Qilin Tang, Benjamin J. Tuck, Sophie Keeling, Daniel Rüdiger, Negar Mirzazadeh Dizaji, Hossein Mohammad-Beigi, Brigitte Nuscher, Pijush Chakraborty, Duncan S. Sutherland, William A. McEwan, Thomas Köglsperger, Stefan Zahler, Markus Zweckstetter, Stefan F. Lichtenthaler, Wolfgang Wurst, Sigrid Schwarz, Günter Höglinger

“…A neuronal eTau uptake and accumulation assay was developed for human induced pluripotent stem cell-derived neurons (iPSCNs) and Lund human mesencephalic cells (LUHMES)-derived neurons. …”
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Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells by Yeon-Ji Lee, In-Won Lee, Tae-Suk Kim, Bo-Gyeong Seo, Sang-Ki Baek, Cheol Hwangbo, Joon-Hee Lee

“…Background: Pluripotent stem cells (PSCs) are capable of differencing into various cell types in the body, providing them valuable for therapy of degenerative diseases. …”
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