Mitochondrial Dynamics: In Cell Reprogramming as It Is in Cancer by Javier Prieto, Josema Torres

“…Somatic cells can be reprogrammed into a pluripotent cellular state similar to that of embryonic stem cells. …”
Get full text

Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells by Gergo Kovacs, Viktoria Szabo, Melinda K. Pirity

Get full text

Cerebral organoids: a promising model in cellular technologies by T. A. Shnaider

“…Finally, we regard existing problems and prospects for development of human pluripotent stem cell-derived threedimensional cerebral organoid technology.…”
Get full text

Mitochondrial Heterogeneity: Evaluating Mitochondrial Subpopulation Dynamics in Stem Cells by D. C. Woods

“…Moreover, contemporary studies evaluating the role of mitochondria in pluripotent stem cells, cellular reprogramming, and differentiation point to a potential importance of mitochondrial subpopulations and heterogeneity in the field of stem cell biology. …”
Get full text

Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding by Debora Salerno, Alessandro Rosa

“…Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. …”
Get full text

Cellular Programming and Reprogramming: Sculpting Cell Fate for the Production of Dopamine Neurons for Cell Therapy by Julio C. Aguila, Eva Hedlund, Rosario Sanchez-Pernaute

“…Pluripotent stem cells are regarded as a promising cell source to obtain human dopamine neurons in sufficient amounts and purity for cell replacement therapy. …”
Get full text

Autophagy in Stem Cell Biology: A Perspective on Stem Cell Self-Renewal and Differentiation by Xihang Chen, Yunfan He, Feng Lu

“…In this review, we describe the most significant advances in the understanding of autophagy regulation in hematopoietic and mesenchymal stem cells, as well as in induced pluripotent stem cells. In particular, we highlight the roles of various autophagy activities in the regulation of self-renewal and differentiation of these stem cells.…”
Get full text

Tumorigenic and Differentiation Potentials of Embryonic Stem Cells Depend on TGFβ Family Signaling: Lessons from Teratocarcinoma Cells Stimulated to Differentiate with Retinoic Aci... by Olga Gordeeva, Sergey Khaydukov

“…A significant challenge for the development of safe pluripotent stem cell-based therapies is the incomplete in vitro differentiation of the pluripotent stem cells and the presence of residual undifferentiated cells initiating teratoma development after transplantation in recipients. …”
Get full text

Novel, low-cost bioreactor for in vitro electrical stimulation of cardiac cells by Joseph P. Licata, Jonathan A. Gerstenhaber, Peter I. Lelkes

“…However, there are discrepancies in the literature as to best practices for promoting cardiac differentiation using ES.MethodsThis study presents a novel, 3D printed bioreactor that delivers in vitro ES to human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), promoting cell maturity and functional readiness for implantation. …”
Get full text

Generation of donor organs in chimeric animals via blastocyst complementation by T. I. Babochkina, L. A. Gerlinskaya, M. P. Moshkin

“…The technologies of producing induced pluripotent stem cells give us the opportunity to obtain human donor cells capable of populating a “free niche”. …”
Get full text

Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells by Lisa Nguyen, Lucas-Sebastian Spitzhorn, James Adjaye

“…Urine has become the source of choice for noninvasive renal epithelial cells and renal stem cells which can be used for generating induced pluripotent stem cells. The aim of this study was to generate a 3D nephrogenic progenitor cell model composed of three distinct cell types—urine-derived SIX2-positive renal progenitor cells, iPSC-derived mesenchymal stem cells, and iPSC-derived endothelial cells originating from the same individual. …”
Get full text

Modeling the Effect of Cannabinoid Exposure During Human Neurodevelopment Using Bidimensional and Tridimensional Cultures by Enrique Estudillo, Jorge Iván Castillo-Arellano, Emilio Martínez, Edgar Rangel-López, Adolfo López-Ornelas, Roxana Magaña-Maldonado, Laura Adalid-Peralta, Iván Velasco, Itzel Escobedo-Ávila

“…Recent studies shed light on this issue by using pluripotent stem cells of human origin, which can recapitulate human neurodevelopment. …”
Get full text

Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cell... by Dong-Seol Lee, Yeo Joon Song, Hye Ri Gug, Ji-Hyun Lee, Hyun Sook Bae, Joo-Cheol Park

“…After an indefinite number of divisions, pluripotent cells differentiate into various types of cells. …”
Get full text

Characterization of Human Mesenchymal Stem Cells Isolated from the Testis by Letizia De Chiara, Elvira Smeralda Famulari, Sharmila Fagoonee, Saskia K. M. van Daalen, Stefano Buttiglieri, Alberto Revelli, Emanuela Tolosano, Lorenzo Silengo, Ans M. M. van Pelt, Fiorella Altruda

“…Spontaneously arising pluripotent stem cells can be obtained in culture from murine spermatogonial stem cells (SSCs), while the pluripotency of the human counterpart remains a matter of debate. …”
Get full text

Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide. by Justyna Jarczak, Kamila Bujko, Katarzyna Brzeźniakiewicz-Janus, Mariusz Ratajczak, Magdalena Kucia

“…Populations of very small embryonic-like stem cells (VSELs) (CD34+lin-CD45- and CD133+lin-CD45-), circulating in the peripheral blood of adults in small numbers, have been identified in several human tissues and together with the populations of hematopoietic stem cells (HSCs) (CD34+lin-CD45+) and CD133+lin-CD45+constitute a pool of cells with self-renewal and pluripotent stem cell characteristics. Using advanced cell staining and sorting strategies, we isolated populations of VSELs and HSCs for bulk RNA-Seq analysis to compare the transcriptomic profiles of both cell populations. …”
Get full text

zIncubascope: Long-term quantitative imaging of multi-cellular assemblies inside an incubator. by Anirban Jana, Naveen Mekhileri, Adeline Boyreau, Aymerick Bazin, Nadège Pujol, Kevin Alessandri, Gaëlle Recher, Pierre Nassoy, Amaury Badon

“…We exemplify the capabilities of our system by investigating human induced pluripotent stem cells (hiPSCs) enclosed in spherical capsules, hiPSCs in tubular capsules and yeast cells in spherical capsules. …”
Get full text

Changes in the Expression of Mitochondrial Morphology-Related Genes during the Differentiation of Murine Embryonic Stem Cells by Jeong Eon Lee, Bong Jong Seo, Min Ji Han, Yean Ju Hong, Kwonho Hong, Hyuk Song, Jeong Woong Lee, Jeong Tae Do

“…Here, we investigated the expression patterns of mitochondria-related genes during the differentiation of mouse embryonic stem cells (ESCs). Pluripotent ESCs maintain stemness in the presence of leukemia inhibitory factor (LIF) via the JAK-STAT3 pathway but lose pluripotency and differentiate in response to the withdrawal of LIF. …”
Get full text

Rescue of in vitro models of CSF1R-related adult-onset leukodystrophy by iluzanebart: mechanisms and therapeutic implications of TREM2 agonism by Kelley C. Larson, Frederick W. Gergits, Abigail J. Renoux, Elizabeth J. Weisman, Borislav Dejanovic, Liyue Huang, Bhaumik Pandya, Donald G. McLaren, Berkley A. Lynch, Richard Fisher, Evan Thackaberry, David Gray, Francois Gaudreault, Christian Mirescu

“…Iluzanebart treatment rescued viability of human monocyte-derived macrophages (hMDM) and induced pluripotent stem cell-derived human microglia (iMGL) in multiple in vitro models of CSF1R-ALSP, including in induced pluripotent stem cell (iPSC) differentiated microglia carrying the heterozygous I794T mutation found in CSF1R-ALSP patients. …”
Get full text

Current Bioengineering Methods for Whole Kidney Regeneration by Shuichiro Yamanaka, Takashi Yokoo

“…Here, we review recent advances in the field of kidney regeneration, including (i) the directed differentiation of induced pluripotent stem cells/embryonic stem cells into kidney cells; (ii) blastocyst decomplementation; (iii) use of a decellularized cadaveric scaffold; (iv) embryonic organ transplantation; and (v) use of a nephrogenic niche for growing xenoembryos for de novo kidney regeneration from stem cells. …”
Get full text

Nuclear Reprogramming in Mouse Primordial Germ Cells: Epigenetic Contribution by Massimo De Felici

“…The status of genome acquired during reprogramming and the associated expression of key pluripotency genes render PGCs susceptible to transform into pluripotent stem cells. …”
Get full text