Insights from a Genome-Wide Study of <i>Pantoea agglomerans</i> UADEC20: A Promising Strain for Phosphate Solubilization and Exopolysaccharides Production by Edith Elizondo-Reyna, Humberto Martínez-Montoya, Yahaira Tamayo-Ordoñez, María Antonia Cruz-Hernández, Mauricio Carrillo-Tripp, María Concepción Tamayo-Ordoñez, Gerardo de Jesús Sosa-Santillán, José Antonio Rodríguez-de la Garza, Mario Hernández-Guzmán, Virgilio Bocanegra-García, Erika Acosta-Cruz

“…The genome was assembled into 13 scaffolds, constituting a total chromosome size of 4.2 Mbp, with two of the scaffolds representing closed plasmids. The strain exhibits activity in phosphate solubilization and exopolysaccharide (EPS) production and secretion; therefore, we explored its biotechnological potential via its genome sequencing and annotation. …”
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Current trends of Carbapenemase Production Amongst Multi-drug Resistant Gram-Negative Bacteria in a Tertiary-Care Hospital in West Bengal by Arunima Chakravarty, Raja Ray, Ananda S. Mondal, Hirak J. Raj

“… Background: Carbapenemase production, being encoded on plasmids, can lead to transferable resistance and outbreaks with a community/hospital setup. …”
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Overexpression of PLCG2 and TMEM38A inhibit tumor progression in clear cell renal cell carcinoma by Yiqiao Zhao, Liang Yang, Xiaojie Bai, Lu Du, Huan Lai, Yiyang Liu, Ping Chen, Michael E. DiSanto, Xinhua Zhang

“…Overexpression models of PLCG2 and TMEM38A were generated in Caki-1 and 786-O cell lines using plasmids. The in vitro experiments demonstrated that both of them exerted pro-apoptotic effects on Caki-1 and 786-O cells, inducing G2/M phase arrest, inhibiting proliferation, and suppressing EMT. …”
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Clinical and genetic analysis of a Chinese family with GM1 gangliosidosis caused by a novel mutation in GLB1 gene by Biao Zhang, Xiao-li Huang, Xin-xin Lu, Heng-bin Huang, Yan-an Wu

“…Enzyme activity of β-galactosidase was detected in 293T cells transiently transfected with plasmids encoding the detected mutations.ResultsTwo siblings in this family presented with neurological degeneration, and were classified as the late-infantile type. …”
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Cytokine-armed pyroptosis induces antitumor immunity against diverse types of tumors by Sara Orehek, Taja Železnik Ramuta, Duško Lainšček, Špela Malenšek, Martin Šala, Mojca Benčina, Roman Jerala, Iva Hafner-Bratkovič

“…We demonstrate that the electrogenic transfer of ICD effector-encoding plasmids into mouse melanoma tumors when combined with intratumoral expression of cytokines IL-1β, IL-12, or IL-18, enhanced anti-tumor immune responses. …”
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Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals by Jinhui Cui, Haixin Cui, Yan Wang, Changjiao Sun, Kui Li, Hongyan Ren, Wei Du

“…Agarose gel electrophoresis experiments show that DNA plasmids can be protected effectively against degradation of exonuclease and endonuclease. …”
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Antibiotic resistance gene occurrence in poultry farms in northeast Brazil by Henrique Francisco de Almeida, Paulo Ricardo Conceição Marques Trindade, César Roberto Viana Teixeira, Claudson Oliveira Brito, Silvio Santana Dolabella, Sona Jain, Maíra Pompeu Martins, Ana Andréa Barbosa

“…Furthermore, similarity was observed for resistance determinants located both on the chromosome and on plasmids, transposons, and integrons. Our results indicate the potential of poultry farming for the environmental dissemination of ARGs in the State of Sergipe. …”
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DNA-based hydrogels for bone regeneration: A promising tool for bone organoids by Xiang Wu, Yan Hu, Shihao Sheng, Huijian Yang, Zuhao Li, Qinglin Han, Qin Zhang, Jiacan Su

“…We then delineate three critical elements in the promotion of bone regeneration using DNA-based hydrogels: (i) osteogenesis driven by phosphate ions, plasmids, and oligodeoxynucleotides (ODNs) that enhance mineralization and promote gene and protein expression; (ii) vascularization facilitated by tetrahedral DNA nanostructures (TDNs) and aptamers, which boosts gene expression and targeted release; (iii) immunomodulation achieved through loaded factors, TDNs, and bound ions that stimulate macrophage polarization and exhibit antibacterial properties. …”
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Global phylogeography and genetic characterization of carbapenem and ceftazidime-avibactam resistant KPC-33-producing Pseudomonas aeruginosa by Longjie Zhou, Jiayao Yao, Ying Zhang, Xiaofan Zhang, Yueyue Hu, Haiyang Liu, Jintao He, Yunsong Yu, Minhua Chen, Yuexing Tu, Xi Li

“…Whole-genome sequencing indicated that bla KPC-33 was located on two Tn4401-like transposons contained in the plasmids and that most of these plasmids could be transferred into P. aeruginosa PAO1Rif isolates. …”
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PMA Induces Vaccine Adjuvant Activity by the Modulation of TLR Signaling Pathway by Dool-Ri Oh, Hu Won Kang, Jong-Ro Kim, Sunoh Kim, In-Kyu Park, Joon Haeng Rhee, Won Keun Oh, Young Ran Kim

“…To identify ligands that potentiate the adjuvant activity of flagellin, we screened a plant library using HEK293T cells transiently cotransfected with phTLR5 and pNF-κB-SEAP plasmids. The 90% EtOH extract from Croton tiglium showed significant NF-κB transactivation in a TLR5-independent manner along with the increase of a flagellin activity. …”
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Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells by Fu-qing Zhong, Yang Li, Xian-qiang Mi

“…Study Design/Materials and Methods. Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells. …”
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Transgene Pyramiding of the HVA1 and mtlD in T3 Maize (Zea mays L.) Plants Confers Drought and Salt Tolerance, along with an Increase in Crop Biomass by Thang Xuan Nguyen, Truong Nguyen, Hussien Alameldin, Benjamin Goheen, Wayne Loescher, Mariam Sticklen

“…The pBY520 containing the Hordeum vulgare  HVA1 regulated by the rice actin promoter (Act1 5′) or the JS101 containing the bacterial mannitol-1-phosphate dehydrogenase (mtlD) also regulated by rice Act1 5′ and a combination of these two plasmids were transferred into the maize genome, and their stable expressions were confirmed through fourth generations. …”
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Heterogeneous phenotype of a Chinese Familial WHIM syndrome with CXCR4V340fs gain-of-function mutation by Yu Huang, Yu Huang, Yu Huang, Lu Li, Lu Li, Ran Chen, Ran Chen, Lang Yu, Lang Yu, Shunkai Zhao, Yanjun Jia, Yanjun Jia, Ying Dou, Ying Dou, Ying Dou, Zhiyong Zhang, Zhiyong Zhang, Yunfei An, Yunfei An, Xuemei Tang, Xuemei Tang, Xiaodong Zhao, Xiaodong Zhao, Lina Zhou

“…Additionally, the frameshift variant CXCR4V340fs led to impaired receptor downregulation in patients’ PBMCs, and in HEK293T cells transfected with the variant plasmids.ConclusionsOur study provided detailed clinical features of four CXCR4V340fs WHIM patients from one Chinese family who presented atypical phenotype and enrich the spectrum of WHIM syndrome.…”
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Metagenomic insights into pathogenic bacteria and antimicrobial resistance in offshore coastal sediments – An example from Gulf waters by Nazima Habibi, Saif Uddin, Hanan Al Sarawi, Ahmad Aldhameer, Montaha Behbehani, Saja Fakhraldeen, Waleed Al-Zekri, Nasreem Abdulrazzack, Farhana Zakir, Anisha Shajan

“…The raw sequencing reads matched with 667 ARGs, 1606 plasmids, 167 integrons and 2143 insertion sequences. …”
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LINC02282 promotes DNA methylation of TRIM6 by recruiting DNMTs to inhibit the progression of Parkinson's disease by Lu Han, Chuansheng Zhao, Feng Jin, Rongfeng Jiang, Hao Wu

“…LINC02282 overexpression plasmids inhibited apoptosis and promoted the proliferation of SH-SY5Y and SK-N-AS cells. …”
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Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal by Rúben Fernandes, Paula Amador, Carla Oliveira, Cristina Prudêncio

“…Of the 193 isolates 81.3% presented transferable plasmids harboring blaESBL genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. …”
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Isoviolanthin promotes Schwann cells activity in peripheral nerve regeneration via Fhl3-mediated epithelial-mesenchymal transition-like process: An in vitro study by Yajuan Su, Tiantian Liu, Minjun Zhao, Dandan Wu, Yuehua Wang, Xubo Wu

“…Schwann cells were transduced with virus particles made in HEK-293T/17 cells by transfection with lentivirus packaging plasmids containing Fhl3. A notable enhancement in Schwann cell proliferation and migration was observed following transduction. …”
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Elucidation of peptide screen for targeted identification of Yersinia pestis by nano-liquid chromatography tandem mass spectrometry by Priya Rani, Syed Imteyaz Alam, Sandeep Singh, Subodh Kumar

“…Additionally, 148 peptides were discovered from proteins of Y. pestis-specific plasmids and chromosomal-associated virulence markers. …”
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Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective β-Estradiol-Degrading Bacterium by Hongyan Zhao, Kejian Tian, Qing Qiu, Yu Wang, Hongyan Zhang, Shuang Ma, Shenbao Jin, Hongliang Huo

“…The genome of Rhodococcus strain DSSKP-R-001 consists of three replicons: one chromosome and two plasmids of 5.2, 0.09, and 0.09, respectively. The results showed that there were ten steroid-degrading enzymes distributed in the whole genome of the strain. …”
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Utility of Lymphoblastoid Cell Lines for Induced Pluripotent Stem Cell Generation by Satish Kumar, Joanne E. Curran, David C. Glahn, John Blangero

“…Here, we report a new optimized LCL-to-iPSC reprogramming protocol using episomal plasmids encoding pluripotency transcription factors and mouse p53DD (p53 carboxy-terminal dominant-negative fragment) and commercially available reprogramming media. …”
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