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321
USP22 inhibits microglial M1 polarization by regulating the PU.1/NLRP3 inflammasome pathway
Published 2025-01-01“…Methods: The BV-2 mouse microglia line was cultured in vitro, and plasmid and siRNA transfection was performed to overexpress or knockdown USP22. …”
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322
Emergence of mcr-8.1-bearing MDR-hypervirulent Klebsiella pneumoniae ST307
Published 2025-02-01“…In addition, the Armenian isolates obtained plasmids carrying virulence and AMR genes during the transmission event. …”
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323
DNGR-1 limits Flt3L-mediated antitumor immunity by restraining tumor-infiltrating type I conventional dendritic cells
Published 2021-05-01“…To overexpress Flt3L systemically, we performed gene therapy through the hydrodynamic injection of an Flt3L-encoding plasmid. To characterize the immune response, we performed flow cytometry and RNA-Seq of tumor-infiltrating cDC1s.Results Here, we found that cross-presentation of tumor antigens in the steady state was DNGR-1-independent. …”
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324
PMAIP1 regulates the progression of follicular thyroid carcinoma through the Wnt3/FOSL1 pathway
Published 2025-01-01“…Following the establishment of a stably transfected plasmid in cell lines, a series of functional assays and subcutaneous xenograft experiment were conducted to investigate the role of PMAIP1 in FTC. …”
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325
Genomic and resistome analysis of Salmonella enterica isolates from retail markets in Yichun city, China
Published 2025-06-01“…The IncR, IncFIB (K), and IncX1 plasmid replicons were widely prevalent and served as significant reservoirs of horizontally acquired foreign genes. …”
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326
Current data on the circulation of the Q fever pathogen in the Republic of Guinea
Published 2024-11-01“…Employing molecular methods, C. burnetii strains carrying the QpH1 plasmid, capable of causing diseases in humans and animals were identified. …”
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327
Construction and Pathogenicity Analysis of a GFPuv-labeled Peanut Ralstonia solanacearum Strain
Published 2024-11-01“…【Method】Through whole-genome analysis of R. solanacearum PeaFJ1 strain, the insertion sites for exogenous gene were selected. The suicide plasmid pK18mobsacB and homologous recombination double exchange technology were employed to integrate the GFPuv gene into the designated site of PeaFJ1 genome. …”
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328
Identification of a novel heterozygous GPD1 missense variant in a Chinese adult patient with recurrent HTG-AP consuming a high-fat diet and heavy smoking
Published 2025-01-01“…In vitro, the corresponding plasmid was constructed and transfected into human renal HEK-293T cells, and GPD1 protein levels were detected. …”
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329
Impact of Novel Resistance Profiles in HIV-1 Reverse Transcriptase on Phenotypic Resistance to NVP
Published 2012-01-01“…Methods. 646 bp HIV-1 pol gene fragments (from 592 to 1237 nucleotide) with different NNRTIs mutation profiles from AIDS patients receiving antiretroviral therapy containing NVP regimens were introduced into pNL4-3 backbone plasmid. H221Y and (or) Y181C mutations were reverted to wild type amino acids by site-directed mutagenesis, then strains containing various mutation patterns were packaged. …”
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330
Exploring the genomic and antimicrobial resistance tapestry: comparative insights into Salmonella enterica serotypes Agona, Braenderup, Muenchen, and Panama in Latin American surfa...
Published 2025-02-01“…The principal coordinates analysis reveals distinct clustering patterns based on country, serotype, number of ARGs per isolate, and plasmid and integron presence/absence. The whole-genome phylogenetic analysis demonstrates clear clusters, each associated with their respective countries. …”
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331
Electroacupuncture Relieves Suppression of Autophagy in Interstitial Cells of Cajal of Diabetic Gastroparesis Rats
Published 2020-01-01“…When ICCs were transfected with GFP-RFP-LC3 plasmid, autophagy flux was observed by laser scanning confocal microscope. …”
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332
Identification of serotype O3b and high-risk clone ST37 of Klebsiella pneumoniae revealed by comparative genomic analysis
Published 2025-01-01“…The ST515 sequence type was present in two K. pneumoniae isolates (CIFT-K7 and CIFT-K8), but the O3b serotype and ST192 allele type were present in the CIFT-K10 isolate.ConclusionTo the best of our knowledge, this research study represents the first Indian report of K. pneumoniae linked to fish, specifically the high-risk clone ‘ST37’ and two other STs, 515 and 192. The most common plasmid type detected in all four isolates was IncFIB, and 75% of the isolates were IncFII and IncHI1B. …”
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333
Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Inhibit Apoptosis of Pulmonary Microvascular Endothelial Cells in COPD Mice Through miR-30b/Wnt5a Pathway
Published 2025-01-01“…We transfected PMVECs with the miR-30b mimic and Wnt5a overexpression plasmid to assess the underlying mechanisms. Cigarette smoke extract (CSE)-induced COPD mice were treated with exosomes and HBLV-mmu-miR-30b via intratracheal instillation. …”
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334
Characterization of Shigella flexneri serotype 6 strains from geographically diverse low- and middle-income countries
Published 2025-01-01“…Complete sequencing of 11 clinical Sf6 strains, demonstrated that the Sf6 virulence plasmid (pINV) is ~38 kb smaller than the average non-Sf6 pINV (~228 kb). …”
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335
In vitro functional validation of anti-CD19 chimeric antigen receptor T cells expressing lysine-specific demethylase 1 short hairpin RNA for the treatment of diffuse large B cell l...
Published 2025-01-01“…The MFG-LSD1 shRNA anti-CD19 CAR plasmid was first constructed, then packaged in retroviral vectors and transduced into human primary peripheral blood mononuclear cell-derived T cells to generate the corresponding CAR-T cells. …”
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336
Safety, Tolerability, and Immunogenicity of the Invaplex<sub>AR-Detox</sub> <i>Shigella</i> Vaccine Co-Administered with the dmLT Adjuvant in Dutch and Zambian Adults: Study Protoc...
Published 2025-01-01“…Invaplex<sub>AR-Detox</sub> is an injectable Shigella vaccine that uses a novel combination of conserved invasion plasmid antigen proteins and a serotype-specific bacterial lipopolysaccharide attenuated for safe intramuscular administration. …”
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337
Microbiology /
Published 2005Table of Contents: “…The history and scope of microbiology -- The study of microbial structure: microscopy and specimen preparation -- Procaryotic cell structure and function -- Eucaryotic cell structure and function -- Microbial nutrition -- Microbial growth -- Control of microorganisms by physical and chemical agents -- Metabolism: energy, enzymes, and regulation -- Metabolism: energy release and conservation -- Metabolism: the use of energy in biosynthesis -- Genes: structure, replication, and mutation -- Genes: expression and regulation -- Microbial recombination and plasmids -- Recombinant DNA technology -- Microbial Genomics -- The viruses: introduction and general characteristics -- The viruses: bacteriophages -- The viruses: viruses of eucaryotes -- Microbial taxonomy -- The archaea -- Bacteria: the deinococci and nonproteobacteria gram negatives -- Bacteria: the proteobacteria -- Bacteria: the Low G 1 C gram positives -- Bacteria: the High G 1 C gram positivies -- The fungi (eumycota), slime molds, and water molds -- The algae -- The protozoa -- Microorganism interactions and microbial ecology -- Microorganisms in aquatic environments -- Microorganisms in terrestrial environments -- Normal microbiota and nonspecific host resistance -- Specific immunity -- Medical immunology -- Pathogenicity of microorganisms -- Antimicrobial chemotherapy -- Clinical microbiology -- The epidemiology of infectious disease -- Human diseases caused by viruses -- Human diseases caused by bacteria -- Human diseases causedd by fungi and protozoa -- Microbiology of food -- Industrial microbiology and biotechnology.…”
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338
Alcohol intake exacerbates experimental autoimmune prostatitis through activating PI3K/AKT/mTOR pathway-mediated Th1 differentiation
Published 2025-01-01“…In contrast, PI3K inhibitor LY294002 and shRNA-PI3K plasmid inhibited PI3K/AKT/mTOR pathway activation, reduced Th1 cell differentiation, and alleviated EAP inflammation severity, respectively.ConclusionOur study is the first to demonstrate that alcohol intake promotes Th1 cell differentiation and exacerbates EAP by activating the PI3K/AKT/mTOR pathway. …”
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339
Variant rs4149584 (R92Q) of the TNFRSF1A gene in patients with familial multiple sclerosis
Published 2025-01-01“…We also conducted a functional study to analyze the pathogenic mechanism of variant rs4149584 (-g.6442643C > G, NM_001065.4:c.362 G > A, R92Q) by plasmid transfection into human oligodendroglioma (HOG) cells, which behave like oligodendrocyte lineage cells; protein labeling was used to locate the protein within cells. …”
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340
Delivery of dendritic cells targeting 3M2e-HA2 nanoparticles with a CpG adjuvant via lysosomal escape of Salmonella enhances protection against H9N2 avian influenza virus
Published 2025-01-01“…Finally, a synthesized 20-repeat CpG adjuvant gene fragment was inserted into pYL327, resulting in the plasmid pYL331. All the constructed plasmids were then transformed into the sifA gene-deficient Salmonella vector χYL56 for oral immunization.The results showed that sifA-deficient Salmonella could efficiently increase antigen-specific mucosal sIgA antibody titers, especially in alveolar lavage samples, whereas the presence of the phage-54 nanobody could dramatically increase intracellular IFN-γ mRNA levels, indicating its ability to enhance the Th1-type immune response. …”
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