Evaluating the health risk of probiotic supplements from the perspective of antimicrobial resistance by Qiwen Tian, Hailv Ye, Xuan Zhou, Junyi Wang, Lifang Zhang, Wenxuan Sun, Chenxin Duan, Minyu Fan, Wei Zhou, Chuyun Bi, Qiong Ye, Aloysius Wong

“…Building on our previous study that reported multiple antibiotic resistance in probiotics of health supplements, in this research, we have attempted to detect their ARGs that may account for resistant phenotypes. ARGs responsible for tetracycline, macrolide, aminoglycoside, and glycopeptide resistance were prevalent in probiotics. …”
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Identification of patient demographic, clinical, and SARS-CoV-2 genomic factors associated with severe COVID-19 using supervised machine learning: a retrospective multicenter study by Kuganya Nirmalarajah, Patryk Aftanas, Shiva Barati, Emily Chien, Gloria Crowl, Amna Faheem, Lubna Farooqi, Alainna J. Jamal, Saman Khan, Jonathon D. Kotwa, Angel X. Li, Mohammad Mozafarihashjin, Jalees A. Nasir, Altynay Shigayeva, Winfield Yim, Lily Yip, Xi Zoe Zhong, Kevin Katz, Robert Kozak, Andrew G. McArthur, Nick Daneman, Finlay Maguire, Allison J. McGeer, Venkata R. Duvvuri, Samira Mubareka

“…Therefore, we aimed to establish a COVID-19 patient dataset with linked clinical and viral genomic data to then examine associations between SARS-CoV-2 genomic signatures and clinical disease phenotypes. Methods A cohort of adult patients with laboratory confirmed SARS-CoV-2 from 11 participating healthcare institutions in the Greater Toronto Area (GTA) were recruited from March 2020 to April 2022. …”
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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma by Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, Solange Landreville, Miguel N. Burnier, Julia V. Burnier

“…We describe efficient methods for the isolation and growth of NCMs and report their phenotypic, proteomic, and transcriptomic characteristics, which will facilitate the investigation of UM development and progression. …”
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Sex differences in epigenetic ageing for older people living with HIVResearch in context by Carrie D. Johnston, Alina P.S. Pang, Eugenia L. Siegler, Charlene Thomas, Chelsie O. Burchett, Mia Crowley, Rochelle O'Brien, Lishomwa C. Ndhlovu, Marshall J. Glesby, Michael J. Corley

“…Hence, we generated a more representative epigenetic dataset to examine sex differences in epigenetic ageing and relationships to clinical phenotypes and proteomics. Methods: We calculated first, second, and third-generation epigenetic ages using DNA methylation data in an observational cohort of 52 females and 106 males with HIV age 50 and over. …”
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Unraveling the immunological landscape and gut microbiome in sepsis: a comprehensive approach to diagnosis and prognosisResearch in context by Yali Luo, Jian Gao, Xinliang Su, Helian Li, Yingcen Li, Wenhao Qi, Xuling Han, Jingxuan Han, Yiran Zhao, Alin Zhang, Yan Zheng, Feng Qian, Hongyu He

“…In addition, T cell phenotypes within adaptive immunity are correlated with sepsis severity and may serve as reliable prognostic markers. …”
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Single-Nucleus RNA Sequencing Reveals Cellular Transcriptome Features at Different Growth Stages in Porcine Skeletal Muscle by Ziyu Chen, Xiaoqian Wu, Dongbin Zheng, Yuling Wang, Jie Chai, Tinghuan Zhang, Pingxian Wu, Minghong Wei, Ting Zhou, Keren Long, Mingzhou Li, Long Jin, Li Chen

“…Numerous studies have highlighted the heterogeneous nature of skeletal muscle, with phenotypic differences reflecting variations in cellular composition and transcriptional profiles. …”
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Modeling feed efficiency over productive lifetime and integrating a submodel for body reserve management in Nordic dairy cattle by R.B. Stephansen, J. Lassen, V.M. Thorup, B.G. Poulsen, J. Jensen, G. Sahana, O.F. Christensen

“…Those results suggest the importance of recording phenotypes in most time periods within and across lactations. …”
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Association of pituitary neuroendocrine tumors and neurofibromatosis type 1: assessing causation versus coincidence. Case report by Mercedes Aguilar-Soto, Julia M. Zuarth-Vázquez, Laura Leyva-Figueroa, Karla Zarco-Ávila, Armando Gamboa-Domínguez, Aldo Eguiluz-Melendez, Laura C. Hernández-Ramírez

“…Two individuals developed multiple endocrine neoplasia-like phenotypes but tested negative for other relevant genetic defects.ConclusionsAlthough the association of NF1 and PitNETs could be coincidental, the clinical characteristics of the reviewed cases differ from those of typical incidentalomas. …”
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Comparative Genomics Reveals Evidence of the Genome Reduction and Metabolic Potentials of <i>Aliineobacillus hadale</i> Isolated from Challenger Deep Sediment of the Mariana Trench by Shaofeng Yang, Jie Liu, Yang Liu, Weichao Wu, Jiahua Wang, Yuli Wei

“…Based on the GTDB taxonomy and phenotypic data, strain Lsc_1132^T could represent a novel species of a novel genus, proposed as <i>Aliineobacillus hadale</i> gen. nov. sp. nov. …”
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An Easy and Quick Risk-Stratified Early Forewarning Model for Septic Shock in the Intensive Care Unit: Development, Validation, and Interpretation Study by Guanghao Liu, Shixiang Zheng, Jun He, Zi-Mei Zhang, Ruoqiong Wu, Yingying Yu, Hao Fu, Li Han, Haibo Zhu, Yichang Xu, Huaguo Shao, Haidan Yan, Ting Chen, Xiaopei Shen

“…Among patients forewarned as high risk, similar pathophysiological phenotypes and high mortality were observed in both those subsequently diagnosed as having SS and those without such a diagnosis. …”
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Mitochondrial targeting of Candida albicans SPFH proteins and requirement of stomatins for SDS-induced stress tolerance by Hyunjeong Kim, Marienela Y. Heredia, Xiao Chen, Maisha Ahmed, Mohammad Qasim, Tracy L. Callender, Aaron D. Hernday, Jason M. Rauceo

“…However, a slp2Δ/Δ/slp3Δ/Δ double mutant strain grew poorly in the presence of 0.08% SDS, accumulated intracellular reactive oxidative species, and displayed aberrant ergosterol distribution in the plasma membrane. These phenotypes were not observed in slp2Δ/Δ or slp3Δ/Δ single mutants, indicating a possible indirect genetic interaction between SLP2 and SLP3. …”
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Multidrug-Resistant <i>Acinetobacter baumannii</i>: Risk Factors for Mortality in a Tertiary Care Teaching Hospital by Kristina Černiauskienė, Astra Vitkauskienė

“…Carbapenemase-producing isolates were detected phenotypically. To determine risk factors for in-hospital mortality, logistic regression analysis was performed. …”
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Selection of terminal Piétrain pigs improves vitality and prevalence of congenital defects in crossbred piglets by Wim Gorssen, Wim Gorssen, Carmen Winters, Roel Meyermans, Léa Chapard, Katrijn Hooyberghs, Jaro De Kort, Alice Van den Broeke, Jürgen Depuydt, Steven Janssens, Nadine Buys

“…Moreover, vitality scores were genetically moderately correlated with preweaning mortality (rg=-0.59) and porcine congenital splay leg syndrome (rg=-0.52). Evolutions of phenotypic records and estimated breeding values since the start of the breeding program indicate a positive impact on piglet survival and prevalence of porcine congenital splay leg syndrome. …”
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Autoimmune glial fibrillary acidic protein astrocytopathy misdiagnosed as intracranial infectious diseases: case reports and literature review by Runhua Bai, Runhua Bai, Li An, Wei Du, Zhiwei Wang, Xiaokun Qi, Jianguo Liu, Ming Ren, Yingxin Yu

“…The most common clinical phenotypes among 40 patients were mimicked meningitis, meningoencephalitis, meningoencephalomyelitis, encephalitis and encephalomyelitis.ConclusionA-GFAP-A is a specific autoimmune meningoencephalomyelitis associated with GFAP-IgG, with lesions involved the brain, meninges, and spinal cord. …”
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Characterization of Listeria monocytogenes strains isolated from soil under organic carrot farming by Maciej Nowak, Zbigniew Paluszak, Natalia Wiktorczyk-Kapischke, Katarzyna Grudlewska-Buda, Anna Budzyńska, Joanna Skonieczna-Kurpiel, Ewa Wałecka-Zacharska, Monika Huse-Kutowska, Krzysztof Skowron

“…These rods can vary in phenotypic characteristics and virulence levels.DiscussionThe research conducted allows to draw attention to the occurrence of pathogens, including L. monocytogenes in crops from organic farming. …”
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Dysplasie faciale chez les chimpanzés sauvages de Sebitoli, Parc national de Kibale, Ouganda : la pollution environnementale en cause ? by Sabrina Krief, Jean-Michel Krief, Andrew Seguya

“…Dans le parc national de Kibale, en Ouganda, notre travail d’habituation dans la zone nord de Sebitoli nous a permis d’observer des phénotypes faciaux singuliers. Huit des 80 chimpanzés (Pan troglodytes schweinfurthii) présentent un nez aplati avec des narines réduites ou absentes et une face concave, sans qu’aucune lésion de la peau ne soit visible. …”
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The behavior of the Bacillus probiotic species under conditions of co-cultivation by O. V. Knysh, A. V. Martynov, S. I. Pokhyl, N. I. Skliar

“…The diameters of swimming halos and migration swarms formed by monoand mixed cultures were measured, and the areas covered by them were compared. The phenotypes of the meeting of swarms formed by cultures spotted on the swarm plates at different locations were also investigated. …”
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Autoinducer-2 enhances the defense of Vibrio furnissii against oxidative stress and DNA damage by modulation of c-di-GMP signaling via a two-component system by Heng Zhang, Wenjin Zhao, Wenguang Yang, Huimin Zhang, Xinyu Qian, Kai Sun, Qiao Yang, Xihui Shen, Lei Zhang

“…In contrast with another well-known AI-2 receptor LuxP that recognizes the borated form of AI-2, AsrK is found to show higher affinity with AI-2 under borate-depleted conditions, and thus boron has a negative effect on AI-2 sensing by AsrK in regulation of the biofilm and motility phenotypes. AI-2 binds to the extracytoplasmic dCache_1 domain of AsrK to inhibit its autokinase activity, thus decreasing the phosphorylation level of its cognate response regulator AsrR and activating the phosphodiesterase activity of AsrR to degrade the cellular second messenger cyclic di-GMP (c-di-GMP). …”
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Green Tea Extract (<i>Theaceae</i>; <i>Camellia sinensis</i> L.): A Promising Antimicrobial, Anti-Quorum Sensing and Antibiofilm Candidate Against Multidrug-Resistant <i>Campylobac... by Mona S. Emara, Ahmed M. Ammar, Ashraf M.O. Abdelwahab, Attia A. Elgdawy, Adel Abdelkhalek, Elena Pet, Gabi Dumitrescu, Mirela Ahmadi, Norhan K. Abd El-Aziz

“…Methods: This study investigated the antimicrobial and antibiofilm activities of some herbal extracts against multidrug-resistant (MDR) <i>Campylobacter</i> species recovered from different sources using phenotypic and molecular techniques. Results: The overall <i>Campylobacter</i> species prevalence was 21.5%, representing 15.25% and 6.25% for <i>C. jejuni</i> and <i>C. coli</i>, respectively. …”
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Secreted PD-L1 alleviates inflammatory arthritis in mice through local and systemic AAV gene therapy by Wenjun Li, Wenjun Li, Junjiang Sun, Susi Feng, Ariana La Rosa, Panli Zhang, Eveline Y. Wu, Richard Loeser, Chengwen Li, Chengwen Li, Chengwen Li

“…To study the therapeutic effect of systemic expression of sPD-L1, we administered AAV8/shPD-L1 gene therapy in CIA mice via retro-orbital injection and found significant improvements in joint inflammation and paw swelling, exhibiting similar phenotypes to that in naïve mice. The levels of total immunoglobulin and anti-collagen specific antibodies were lower in AAV8/shPD-L1 treated CIA mice than those in controls. …”
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