Water homeostasis gene expression in the kidney of broilers divergently selected for water conversion ratio by Kentu Lassiter, Loujain Aloui, Elizabeth S. Greene, Marciela Maqaeda, Travis Tabler, Sami Dridi, Robert F. Wideman, Jr., Sara Orlowski, Walter G. Bottje

“…In Exp. 1, male and female HWE and LWE broilers were individually phenotyped between 4 and 6 wks of age to determine growth performance and water conversion ratio (g water intake/g body weight gain). …”
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The Neosartorya (Aspergillus) fischeri antifungal protein NFAP2 has low potential to trigger resistance development in Candida albicans in vitro by Gábor Bende, Nóra Zsindely, Krisztián Laczi, Zsolt Kristóffy, Csaba Papp, Attila Farkas, Liliána Tóth, Szabolcs Sáringer, László Bodai, Gábor Rákhely, Florentine Marx, László Galgóczy

“…In this study, we provide evidence that NFAP2 has a low potential to trigger resistance in C. albicans in vitro, and the developed tolerance to NFAP2 is not associated with severe phenotypic changes compared with development of resistance to generic fluconazole. …”
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Understanding phage BX-1 resistance in Vibrio alginolyticus AP-1 and the role of quorum-sensing regulation by Xiaoyu Li, Xin Liu, Tianyi Ma, Haochen Su, Bingrui Sui, Lili Wang, Bilal Murtaza, Yongping Xu, Na Li, Demeng Tan

“…However, its application is hindered by a limited understanding of the genotypic and phenotypic dynamics governing phage-host interactions. …”
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Clinical, genomic, and histopathologic diversity in cerebral cavernous malformations by Jian Ren, Daochao Wang, Leiming Wang, Chendan Jiang, An Tian, Ziwei Cui, Yeqing Ren, Lisong Bian, Gao Zeng, Guolu Meng, Yongzhi Shan, Jiantao Liang, Xinru Xiao, Jie Tang, Yukui Wei, Chuan He, Liyong Sun, Yongjie Ma, Jiaxing Yu, Guilin Li, Ming Ye, Peng Hu, Jingwei Li, Ye Li, Lijian Niu, Qianwen Li, Feng Ling, Jan-Karl Burkhardt, Hongqi Zhang, Tao Hong

“…We analyzed 290 surgical specimens from symptomatic CCM patients, utilizing whole-exome sequencing, droplet digital PCR, and targeted panel sequencing, alongside immunohistology to examine genotypic and phenotypic differences. Among 290 cases, 201 had somatic MAP3K3, PIK3CA, or germline CCM mutations, each associated with distinct clinical parameters: hemorrhage risk (P < 0.001), lesion size (P = 0.019), non-hemorrhagic epilepsy (P < 0.001), Zabramski classifications (P < 0.001), developmental venous anomaly presence (P < 0.001), and MRI-detected edema (P < 0.001). …”
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Profile of the grain physical traits and physicochemical properties of selected Malaysian rice landraces for future use in a breeding program by Site Noorzuraini Abd Rahman, Rosimah Nulit, Faridah Qamaruz Zaman, Khairun Hisam Nasir, Mohd Hafiz Ibrahim, Mohd Ramdzan Othman, Nur Idayu Abd Rahim, Nor Sufiah Sebaweh

“…The gel consistency exhibited the highest phenotypic and genotypic coefficient of variance, with values of 42.44% and 41.88%, respectively. …”
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High Ki67 expression, HER2 overexpression, and low progesterone receptor levels in high-grade DCIS: significant associations with clinical practice implications by Hossein Schandiz, Hossein Schandiz, Lorant Farkas, Lorant Farkas, Daehoon Park, Yan Liu, Yan Liu, Solveig N. Andersen, Torill Sauer, Jürgen Geisler, Jürgen Geisler

“…Through rigorous analysis of histopathological and immunopathological samples from a substantial cohort, this study revealed robust correlations between heightened Ki67 expression, diminished progesterone (PR) levels, and HER2 overexpression, indicative of aggressive DCIS phenotypes. These findings offer novel insights into the surrogate immunomolecular subtyping landscape of DCIS, potentially refining risk stratification and therapeutic approaches. …”
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The implementation of genome sequencing in rare genetic diseases diagnosis: a pilot study from the Hong Kong genome projectResearch in context by Wai Kei Jacky Lam, Chak Sing Lau, Ho Ming Luk, Lisa Wing Chi Au, Gary C.P. Chan, Will Yap Hang Chan, Shirley Sze Wing Cheng, Timothy Hua Tse Cheng, Li Li Cheung, Yiu Fai Cheung, Josephine Shuk Ching Chong, Annie Tsz Wai Chu, Claudia Ching Yan Chung, Kin Lai Chung, Cheuk Wing Fung, Eva Lai Wah Fung, Yuan Gao, Stephanie Ho, Shirley Pik Ying Hue, Chi-Ho Lee, Tsz Leung Lee, Philip Hei Li, Hei Man Lo, Ivan Fai Man Lo, Herbert Ho Fung Loong, Becky Mingyao Ma, Wei Ma, Shirley Yin Yu Pang, Wai-Kay Seto, Steven Wai Kwan Siu, Ho So, Yuk Him Tam, Wenshu Tang, Rosanna Ming Sum Wong, Desmond Yat Hin Yap, Maggie Lo Yee Yau, Brian Hon Yin Chung, Su-Vui Lo

“…All participants received srGS, while lrGS was applied to a subset to resolve technically challenging regions unclear from srGS and provide phasing information for potential compound heterozygous variants. A phenotypic-driven diagnostic workflow was implemented to filter and prioritise rare and likely disease-causing variants. …”
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Knockout of the inhibitory receptor TIGIT enhances the antitumor response of ex vivo expanded NK cells and prevents fratricide with therapeutic Fc-active TIGIT antibodies by Dean A Lee, Jeremiah L Oyer, Md Faqrul Hasan, Tayler J Croom-Perez, Liza D Robles-Carrillo, Sanjana Kumar, Brendan W Andersen, Brian P Tullius, Alicja J Copik, Amanda R Campbell, Thomas A Dieffenthaller, Catherine A Cash, Jonathan E Eloriaga, Meisam Naeimi Kararoudi

“…Since NK cells express TIGIT and Fc-receptor CD16, Fc-active anti-TIGIT may deplete NK cells via fratricide, which has not been studied.Methods CRISPR-Cas9-based TIGIT knockout (KO) was performed in expanded NK cells. Phenotypic and transcriptomic properties of TIGIT KO and wild-type (WT) NK cells were compared with flow cytometry, CyTOF, and RNA sequencing. …”
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Phase I study of efineptakin alfa (NT-I7) for the treatment of Kaposi sarcoma by Leonard D’Amico, Martin Cheever, Steven P Fling, Anna Wright, Kathryn Lurain, Ramya Ramaswami, Irene Ekwede, Thomas S Uldrick, Robert Yarchoan, Judith C Kaiser, Angela Shaulov Kask, Manoj P Menon, Paul Couey, Eli Burnham, Allysson Angeldekao, Byung Ha Lee, Li-Lian Kwok, Chia-Ching Jackie Wang

“…In PWH, IL-7 administration leads to increased numbers of circulating central memory and naïve T-cell phenotypes.Methods In this multicenter phase I study with a 3+3 dose escalation design, participants with KS with or without HIV received up to four intramuscular injections of IL-7 (NT-I7) every 9 weeks. …”
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Obstructive sleep apnea and structural and functional brain alterations: a brain-wide investigation from clinical association to genetic causality by Kang Wu, Qiming Gan, Yuhong Pi, Yanjuan Wu, Wenjin Zou, Xiaofen Su, Sun Zhang, Xinni Wang, Xinchun Li, Nuofu Zhang

“…Additionally, large-scale Mendelian randomization analyses were performed using genome-wide association study (GWAS) data on OSA and 3935 brain imaging-derived phenotypes (IDPs), assessed in up to 33,224 individuals between December 2023 and March 2024, to explore potential genetic causality between OSA and alterations in whole brain structure and function. …”
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Proteomic analysis to explore potential mechanism underlying pseudomale sperm defect in Cynoglossus semilaevis by Xihong Li, Lu Li, Zhongkai Cui, Ming Li, Xuexue Sun, Zhijie Li, Zhangfan Chen, Lanqing Ding, Dongdong Xu, Wenteng Xu

“…Females of Chinese tongue sole (Cynoglossus semilaevis) grow 2–4 times faster and bigger than males, but some genetic females can sex reverse into phenotypic males (called pseudomales, ZW karyotype) that presents similar slow growth to normal males. …”
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New insights into the immunomodulatory potential of sialic acid on monocyte-derived dendritic cells by Zélia Silva, João Amorim Rabaça, Vanessa Luz, Rita Adubeiro Lourenço, Mariolina Salio, Alexandra Couto Oliveira, Pedro Bule, Sebastian Springer, Paula Alexandra Videira

“…In this study, we focused on the impact of sialic acid remodelling by treatment with Clostridium perfringens sialidase on MoDCs' phenotypic and functional characteristics. Our investigation juxtaposes this novel approach with the conventional cytokine-based maturation regimen commonly employed in clinical settings. …”
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Gut dysbiosis is associated with difficult-to-treat rheumatoid arthritis by Patricia Ruiz-Limón, Patricia Ruiz-Limón, Patricia Ruiz-Limón, Natalia Mena-Vázquez, Natalia Mena-Vázquez, Isabel Moreno-Indias, Isabel Moreno-Indias, Isabel Moreno-Indias, Jose Manuel Lisbona-Montañez, Jose Manuel Lisbona-Montañez, Jose Manuel Lisbona-Montañez, Arkaitz Mucientes, Arkaitz Mucientes, Sara Manrique-Arija, Sara Manrique-Arija, Sara Manrique-Arija, Rocío Redondo-Rodriguez, Rocío Redondo-Rodriguez, Laura Cano-García, Laura Cano-García, Francisco J. Tinahones, Francisco J. Tinahones, Francisco J. Tinahones, Francisco J. Tinahones, Antonio Fernández-Nebro, Antonio Fernández-Nebro, Antonio Fernández-Nebro

“…In 2022, we identified 2 extreme patient phenotypes: (1) D2T RA, which was defined as failure of ≥2 biological or targeted synthetic disease-modifying antirheumatic drugs (b/tsDMARDs) (with different mechanisms of action) plus signs of active disease; and (2) easy-to-treat RA (E2T RA), i.e., stable disease managed with a single treatment. …”
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Assessment of Antibiotic Resistance Among Isolates of <i>Klebsiella</i> spp. and <i>Raoultella</i> spp. in Wildlife and Their Environment from Portugal: A Positive Epidemiologic Ou... by Carolina Sabença, Rani de la Rivière, Paulo Barros, João Alexandre Cabral, Roberto Sargo, Luís Sousa, Maria de Lurdes Enes Dapkevicius, Filipe Silva, Filipa Lopes, Ana Carolina Abrantes, Madalena Vieira-Pinto, Manuela Caniça, Gilberto Igrejas, Carmen Torres, Patrícia Poeta

“…Antibiograms were performed for 15 antibiotics to determine the phenotypic resistance profile in these isolates. Extended-spectrum β-lactamase (ESBL) production was tested by the double-disc synergy test, and one ESBL-producing <i>K. pneumoniae</i> isolate was screened by PCR and whole-genome sequencing. …”
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Genome-wide association mapping and genomic prediction analyses reveal the genetic architecture of grain yield and agronomic traits under drought and optimum conditions in maize by Manigben Kulai Amadu, Yoseph Beyene, Vijay Chaikam, Pangirayi B. Tongoona, Eric Y. Danquah, Beatrice E. Ifie, Juan Burgueno, Boddupalli M. Prasanna, Manje Gowda

“…Among these QTNs, 17 QTNs explained over 10% of the phenotypic variation (R 2  ≥ 10%). Furthermore, 43 candidate genes were discovered and annotated. …”
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Prevalence and Molecular Characterization of Carbapenemase-Producing Multidrug-Resistant Bacteria in Diabetic Foot Ulcer Infections by Mohd Saleem, Soha Abdallah Moursi, Tahani Nasser Almofeed Altamimi, Mohammed Salem Alharbi, Alwaleed Mohammad Alaskar, Sahar Adly Hassan Hammam, Ehab Rakha, Ozair Ilyas Syed Muhammad, Hamoud Abdulmohsin Almalaq, Metab Nasser Alshammari, Azharuddin Sajid Syed Khaja

“…A total of 200 clinical isolates from DFU patients were analyzed via phenotypic assays, including the modified Hodge test (MHT) and the Carba NP test, alongside molecular techniques to detect carbapenemase-encoding genes (<i>blaKPC</i>, <i>blaNDM</i>, <i>blaVIM</i>, <i>blaIMP</i>, and <i>blaOXA-48</i>). …”
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Genotypic difference in response to copper stress in upland cotton as revealed by physiological and molecular expression analyses by Jianfei Wu, Tao Wang, Yin Huang, Shuiping Xiao, Xiaoxia Luo, Yanfeng Deng, Xiu Yang, Qingquan Kong, Feiyu Tang

“…Results Based on the phenotypic values and corresponding tolerance indexes of 12 parameters, A2304 and A1415 were identified as Cu-tolerant and -sensitive, respectively. …”
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Chemotherapy-induced cellular senescence promotes stemness of aggressive B-cell non-Hodgkin’s lymphoma via CCR7/ARHGAP18/IKBα signaling activation by Yangyang Wang, Fan Wu, Yang Wan, Huiping Wang, Linhui Hu, Qianshan Tao, Zhimin Zhai, Jiyu Wang, Keke Huang, Anwen Ren, Jinlan Li, Liuying Yi, Yanjie Ruan, Zhixiang Wanyan, Chaohong Liu

“…Furthermore, B-NHL-TIS regulated by CCR7 and exhibited enhanced phenotypic and functional stemness features, including the upregulation of stemness markers, increased colony-forming, invasive and migratory capabilities. …”
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Antibiotic Susceptibility Patterns and Virulence Profiles of Classical and Hypervirulent <i>Klebsiella pneumoniae</i> Strains Isolated from Clinical Samples in Khyber Pakhtunkhwa,... by Azra, Taj Ali Khan, Ihtisham Ul Haq, Woranich Hinthong, Susana Campino, Aisha Gohar, Noman Khan, Muhammad Kashif, Ihsan Ullah, Taane G. Clark

“…Among these, 64 <i>K. pneumoniae</i> strains were isolated and subsequently subjected to antimicrobial susceptibility testing (AST) and phenotypic virulence detection. Among the 64 isolates, 21 (32.8%) exhibited hypermucoviscosity, a characteristic associated with increased pathogenicity. …”
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Genetic parameters and genome-wide association studies including the X chromosome for various reproduction and semen quality traits in Nellore cattle by Felipe E. de Carvalho, José Bento S. Ferraz, Victor B. Pedrosa, Elisangela C. Matos, Joanir P. Eler, Marcio R. Silva, José D. Guimarães, Fernando Bussiman, Barbara C. A. Silva, Henrique A. Mulim, Artur Oliveira Rocha, Andre C. Araujo, Hui Wen, Gabriel S. Campos, Luiz F. Brito

“…Conclusions Our findings indicate the key role of genes located on the X chromosome on the phenotypic variability of male and female reproduction and fertility traits in Nellore cattle. …”
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