MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA by Swapna C. Senan, Jithu Paul Jacob, Ramani Bhai

“…Result: The twelve isolates resistant to carbapenem were confirmed phenotypically and were confirmed using a multiplex Polymerase Chain Reaction. …”
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Recent advances in adipose-derived mesenchymal stem cell-derived exosomes for regulating macrophage polarization by Zhewei Dong, Yingli Fu, Zhongming Cai, Hao Dai, Yucang He

“…This review summarizes the contents and functions of ADSC-EXOs, outlines their properties and the characteristics of macrophage phenotypes, and emphasizes their impact on macrophage polarization and their contribution to immune-related diseases.…”
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COMPARISON OF THE COMPLETE NUCLEOTIDE SEQUENCES OF MITOCHONDRIAL DNA FROM WISTAR AND OXYS RAT STRAINS MAINTAINED AT THE INSTITUTE OF CYTOLOGY AND GENETICS SB RAS by R. A. Maksyutov, E. V. Gavrilova, T. S. Nepomniashchikh, U. N. Rotskaya, P. S. Loshchenova, N. G. Kolosova, O. I. Sinitsyna, S. N. Shchelkunov

“…It was shown that two additional unique nucleotide substitutions between rats OXYS (ICG) and Wistar (ICG) were absent from other known nucleotide sequences of rat mitochondrial DNA of rats and that they did not cause amino acid substitutions, so that all phenotypic differences between the lines were determined only by differences in their nuclear DNA. …”
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Identification and structure-guided development of triazole urea-based selective antagonists of Arabidopsis karrikin signaling by Jianwen Wang, Ikuo Takahashi, Ko Kikuzato, Toshihiko Sakai, Zhangliang Zhu, Kai Jiang, Hidemitsu Nakamura, Takeshi Nakano, Masaru Tanokura, Takuya Miyakawa, Tadao Asami

“…KK181N1 selectively depress the KAR-induced phenotypes in Arabidopsis. We further elucidate the antagonistic, KAI2 binding mechanism of KK181N1, showing that KK181N1 binds to the catalytic pockets of KAI2 in a non-covalent binding manner. …”
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Acetyl-CoA synthesis in the skin is a key determinant of systemic lipid homeostasis by Phuong T.T. Nguyen, Mia Shiue, Nina Kuprasertkul, Pedro Costa-Pinheiro, Luke T. Izzo, Laura V. Pinheiro, Hayley A. Affronti, Gabriel Gugiu, Shivani Ghaisas, Joyce Y. Liu, Jordan C. Harris, Charles W. Bradley, John T. Seykora, Xiaolu Yang, Taku Kambayashi, Clementina Mesaros, Brian C. Capell, Kathryn E. Wellen

“…Dietary fat supplementation further boosts sebum production and partially rescues both the lipoatrophy and the aberrant skin phenotypes. The data establish a critical role for cytosolic acetyl-CoA synthesis in maintaining skin barrier integrity and highlight the skin as a key organ in systemic lipid regulation.…”
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Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering by Claudio Muscari, Emanuele Giordano, Francesca Bonafè, Marco Govoni, Carlo Guarnieri

“…To this aim, cells with a differentiation potential towards vascular and cardiac muscle phenotypes have been cocultured in 2D or 3D appropriate scaffolds. …”
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Cacao floral traits are shaped by the interaction of flower position with genotype by Seunghyun Lim, Insuck Baek, Seok Min Hong, Yoonjung Lee, Silvas Kirubakaran, Moon S. Kim, Lyndel W. Meinhardt, Sunchung Park, Ezekiel Ahn

“…These findings emphasize the phenotypic diversity of cacao flowers and demonstrate the potential of machine learning in genotype identification, offering valuable insights for breeding and cultivation strategies to enhance cacao productivity.…”
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Research Progress in Diseases Caused by STAT1 Gain-of-Function Mutations by LI Linpeng, MA Jing, GU Hao, SHU Zhou, MAO Huawei

“…With the understanding of disease mechanisms and the discovery of new clinical phenotypes, this review focuses on the diseases caused by gain-of-function mutations of STAT1, and introduces the clinical manifestations and treatment progress of STAT1-GOF to promote the understanding of such diseases and their future diagnosis, treatment and research works.…”
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NLRP3 Deficiency Attenuates Renal Fibrosis and Ameliorates Mitochondrial Dysfunction in a Mouse Unilateral Ureteral Obstruction Model of Chronic Kidney Disease by Honglei Guo, Xiao Bi, Ping Zhou, Shijian Zhu, Wei Ding

“…The severe glomerular injury and tubulointerstitial fibrosis induced in WT mice by UUO was markedly attenuated in NLRP3−/− mice as evidenced by blockade of extracellular matrix deposition, decreased cell apoptosis, and phenotypic alterations. Moreover, NLRP3 deletion reversed UUO-induced impairment of mitochondrial morphology and function. …”
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Single cell suppression profiling of human regulatory T cells by Jonas Nørskov Søndergaard, Janyerkye Tulyeu, David Priest, Shimon Sakaguchi, James B. Wing

“…Additionally, scSPOT identifies a Treg phenotypic split previously observed in viral infection and propose modes of action by the FDA-approved drugs Ipilimumab and Tazemetostat. scSPOT is thus scalable, robust, widely applicable, and may be used to better understand Treg immunobiology and screen for therapeutic compounds.…”
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Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus by Chenglin Sang, Xuecheng Cao, Fangjing Chen, Xuekang Yang, Yongxian Zhang

“…We aimed to figure out whether NP and AF also contain stem cells and whether these stem cells share common properties with chondrocytes and/or fibroblasts in their phenotypes or whether they are completely different types of cells with different characteristics. …”
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Comprehensive Genetic Analysis of Edible-Podded Pea Genotypes: Variability, Heritability, and Multivariate Approach Across Two Agro-Climatic Zones in India by Saurabh Yadav, Rajinder Kumar Dhall, Hira Singh, Parteek Kumar, Priti Sharma, Pradeep Kumar, Priyanka Kumari, Neha Rana

“…Significant variation was observed for most traits, with high genotypic and phenotypic coefficients of variation, heritability, and genetic advance, especially in vigor, yield, and biochemical traits. …”
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From Abnormal Hippocampal Synaptic Plasticity in Down Syndrome Mouse Models to Cognitive Disability in Down Syndrome by Nathan Cramer, Zygmunt Galdzicki

“…While the resulting physiological and behavioral phenotypes vary in their penetrance and severity, all individuals with DS have variable but significant levels of cognitive disability. …”
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Oxidative Stress, DNA Damage, and c-Abl Signaling: At the Crossroad in Neurodegenerative Diseases? by Stefania Gonfloni, Emiliano Maiani, Claudia Di Bartolomeo, Marc Diederich, Gianni Cesareni

“…This suggests that aberrant nonspecific posttranslational modifications induced by c-Abl may contribute to fuel the recurrent phenotypes/features linked to neurodegenerative disorders, such as an impaired mitochondrial function, oxidative stress, and accumulation of protein aggregates. …”
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3D bioprinting for the production of a perfusable vascularized model of a cancer niche by Federico Maggiotto, Federico Maggiotto, Lorenzo Bova, Lorenzo Bova, Lorenzo Bova, Sara Micheli, Sara Micheli, Camilla Pozzer, Pina Fusco, Pina Fusco, Paolo Sgarbossa, Fabrizio Billi, Elisa Cimetta, Elisa Cimetta

“…We used a multi-material and sacrificial bioprinting approach to fabricate vascularized 3D cell-laden structures and developed a customized perfusion system enabling maintenance of growth and viability of the constructs for up to 3 weeks. Cell phenotypes and densities in co-culture for both the bulk of the construct and the endothelialization of the vascular channels were optimized to better replicate in vivo conditions and ideally simulate tumor progression. …”
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RNA helicase MOV10 suppresses fear memory and dendritic arborization and regulates microtubule dynamics in hippocampal neurons by Temirlan Shilikbay, Aatiqa Nawaz, Megan Doon, Stephanie Ceman

“…Restoration of NUMA1 expression and knockdown of HAUS rescued phenotypes of the Mov10 Deletion hippocampal neurons. …”
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A mechanism-informed deep neural network enables prioritization of regulators that drive cell state transitions by Xi Xi, Jiaqi Li, Jinmeng Jia, Qiuchen Meng, Chen Li, Xiaowo Wang, Lei Wei, Xuegong Zhang

“…Some recent neural network models can connect molecular changes to cellular phenotypes, but their design lacks modeling of regulatory mechanisms, limiting the decoding of regulations behind key cellular events, such as cell state transitions. …”
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Recent Insights into Antibiotic Resistance in Helicobacter pylori Eradication by Wenming Wu, Yunsheng Yang, Gang Sun

“…Many factors have been implicated as causes of treatment failure, but the main antibiotic resistance mechanisms described to date are due to point mutations on the bacterial chromosome, a consequence of a significantly phenotypic variation in H. pylori. The prevalence of antibiotic (e.g., clarithromycin, metronidazole, tetracycline, amoxicillin, and furazolidone) resistance varies among different countries; it appears to be partly determined by geographical factors. …”
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Recent Advances in Our Understanding of Human Inflammatory Dendritic Cells in Human Immunodeficiency Virus Infection by Freja A. Warner van Dijk, Kirstie M. Bertram, Thomas R. O’Neil, Yuchen Li, Daniel J. Buffa, Andrew N. Harman, Anthony L. Cunningham, Najla Nasr

“…This increased HIV susceptibility in inflamed tissue likely stems from a disrupted epithelial barrier integrity, phenotypic changes in resident DCs and an influx of inflammatory HIV target cells, including DCs and CD4 T cells. …”
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A study on the production of extracellular vesicles derived from novel immortalized human placental mesenchymal stromal cells by Yingying Tong, Jie Sun, Xin Jiang, Xu Jia, Huimin Xiao, Hua Wang, Guanghua Yang

“…We investigated immortalized placental chorionic mesenchymal stromal cells (imCMSCs), a stromal cell line that surpasses the growth limitations of primary passage cells while retaining phenotypic characteristics and differentiation potential. …”
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