Radiation to all macroscopic sites of tumor permits greater systemic antitumor response to in situ vaccination by Zachary S Morris, Paul M Sondel, Amy K Erbe, Alexander L Rakhmilevich, Amber M Bates, Peter M Carlson, Alexander A Pieper, Ravi B Patel, Jen Birstler, Joseph Grudzinski, Reinier Hernandez, Bryan P Bednarz, Jamey P Weichert, Arika S Feils, Matthew Rodriquez, Claire Sun, Ian Marsh

“…Using flow cytometry, we identified an influx of CD8+ T cells with a less exhausted phenotype in both the ISV-targeted primary and the distant secondary tumor following the combination of secondary tumor EBRT or TRT with primary tumor ISV.Conclusions We report a novel use for low-dose RT, not as a direct antitumor modality but as an immunomodulator capable of driving and expanding antitumor immunity against metastatic tumor sites following ISV.…”
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Molecular hydrogen reduces dermatitis-induced itch, diabetic itch and cholestatic itch by inhibiting spinal oxidative stress and synaptic plasticity via SIRT1-β-catenin pathway in... by Linlin Zhang, Fangshi Zhao, Yize Li, Zhenhua Song, Lingyue Hu, Yuanjie Li, Rui Zhang, Yonghao Yu, Guolin Wang, Chunyan Wang

“…Spinal SIRT1 inhibition eliminates antipruritic and antioxidative effects of hydrogen, while SIRT1 agonism attenuates chronic itch phenotype, spinal β-catenin acetylation and mitochondrial damage. β-catenin inhibitors are effective against chronic itch via reducing β-catenin acetylation, blocking ERK phosphorylation and elevating antioxidant enzymes activity. …”
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Therapeutic effects of cyclophosphamide, dsDNA preparations and combinations thereof against Krebs-2 ascites cancer cells and various cancer transplants by E. A. Potter, E. V. Dolgova, A. M. Minkevich, Ya. R. Efremov, O. S. Taranov, V. V. Omigov, V. P. Nikolin, N. A. Popova, A. S. Proskurina, E. I. Vereschagin, A. V. Kozel, V. A. Rogachev, D. B. Petrov, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, S. S. Bogachev

“…When Krebs-2 ascites are treated with human DNA 1-12 hours post CP injection, this results in either elimination of cells that internalize TAMRA-labeled DNA (TISCs) or alters their phenotype, which is accompanied with the loss of surface expression of CD34. …”
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Immune-checkpoint-inhibitor therapy directed against PD-L1 is tolerated in the heart without manifestation of cardiac inflammation in a preclinical reversible melanoma mouse model by Caroline Schoenherr, Caroline Schoenherr, Stefan Pietzsch, Stefan Pietzsch, Cristina Barca, Franziska E. Müller, Frauke S. Bahr, Martina Kasten, Andre Zeug, Sergej Erschow, Christine S. Falk, Evgeni Ponimaskin, James T. Thackeray, Denise Hilfiker-Kleiner, Denise Hilfiker-Kleiner, Melanie Ricke-Hoch

“…Thus, anti-PD-L1 therapy improved survival in mice with advanced melanoma cancer with no major cardiac phenotype or inflammation 50 days after tumor inoculation. …”
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Thioredoxin-interacting protein (TXNIP) inhibition promotes retinal ganglion cell survival and facilitates M1-like microglial transformation via the PI3K/Akt pathway in glaucoma by Junjue Chen, Huimin Zhong, Bingqiao Shen, Huan Yu, Yang Zhang, Ruiqi Han, Ping Huang, Shouyue Huang, Yisheng Zhong

“…Western blot, RT-PCR and immunofluorescence were used to investigate the proinflammatory cytokines secretion, glucose uptake and phenotype transformation in wild type, TXNIP knockout and overexpressed microglia combined with IL-17A treatment. …”
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Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway by Fengzhen Cui, Shiyu Deng, Yan Fu, Tongtong Xu, Shuangshuang Bao, Siyi Wang, Yahang Lin, Xianghui Wang, Faming Zhao, Tingting Zhang, Shunqing Xu, Zhijun Zhang, Wanlu Li, Guo-Yuan Yang, Huanwen Tang, Jixian Wang, Xia Sheng, Yaohui Tang

“…Conditional knockout or pharmacological inhibition of IRE1α markedly inhibits NSC differentiation into astrocytes and astrocyte reactivity, attenuates synapse phagocytosis, and improves cognitive function. This phenotype is further recapitulated in a human brain organoid model. …”
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Klebsiella pneumoniae in stranded California sea lions (Zalophus californianus) of Central California between 2017-2022 by Sydney N. Zotto, Robin C. Dunkin, Juliana D. Limon, Maia S. Smith, Lisabet M. Hortensius, Margaret E. Martinez, Pádraig J. Duignan, Cara Field, Esteban Soto, Dane Whitaker, Carlos Rios, Maurice Byukusenge, Bhushan Jayarao, Suresh V. Kuchipudi, Amy C. Kinsley

“…A hypermucoviscous (HMV) phenotype of K. pneumoniae is reported in stranded California sea lions (CSL) causing pleuritis and bronchopneumonia as a persistent cause of strandings. …”
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Characterisation of clinical response and transcriptional profiling of proliferating CD8 T cells in the blood of cancer patients after PD-1 monotherapy or combination therapy by Peng Li, Haydn Kissick, Rebecca C Obeng, Tahseen H Nasti, Christiane S Eberhardt, Rafi Ahmed, Zhengjia Chen, Warren J Leonard, Suresh S Ramalingam, Andreas Wieland, Annapaola Mariniello, Jeffrey M Switchenko, Kylee Martens, Daniel Y Chang, Donald McGuire, Candace Daugherty, Yuzi Zhang, Rathi Pillai, Alice O Kamphorst

“…The proliferating cells had an effector-like phenotype. The transcriptional profiles of the effector-like CD8 T cells were similar irrespective of treatment type or clinical response but distinct from that of YFV-specific effector CD8 T cells.Conclusions Our data suggest that early proliferative CD8 T-cell response in the blood is predictive, and that an early-and-sustained proliferative response may further identify patients with prolonged survival. …”
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Immunomodulatory Effects Mediated by Dopamine by Rodrigo Arreola, Samantha Alvarez-Herrera, Gilberto Pérez-Sánchez, Enrique Becerril-Villanueva, Carlos Cruz-Fuentes, Enrique Octavio Flores-Gutierrez, María Eugenia Garcés-Alvarez, Dora Luz de la Cruz-Aguilera, Emilio Medina-Rivero, Gabriela Hurtado-Alvarado, Saray Quintero-Fabián, Lenin Pavón

“…Receptors that are expressed in leukocytes are linked to signaling pathways that are mediated by changes in cAMP concentration, which in turn triggers changes in phenotype and cellular function. According to the leukocyte lineage, the effects of DA are associated with such processes as respiratory burst, cytokine and antibody secretion, chemotaxis, apoptosis, and cytotoxicity. …”
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Ferroptosis-related genes participate in the microglia-induced neuroinflammation of spinal cord injury via NF-κB signaling: evidence from integrated single-cell and spatial transcr... by Siqiao Wang, Li Yang, Zhourui Wu, Chen Li, Shaoke Wang, Zhihui Xiao, Bei Ma, Rongrong Zhu, Liming Cheng

“…Conclusions A novel subcluster of microglia exhibiting M1 polarization and ferroptosis phenotype was involved in SCI. These microglia may trigger neuroinflammation and induce neuronal degeneration within the middle site of SCI, which might be inhibited by NT3-chitosan or LDH-NT3.…”
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Gastrointestinal symptoms and bowel habits in 53 046 healthy Danish blood donors: a nationwide cross-sectional study by Christian Erikstrup, Ole Birger Pedersen, Henrik Hjalgrim, Erik Sørensen, Jens Frederik Dahlerup, Klaus Rostgaard, Mikkel Steen Petersen, Sisse Rye Ostrowski, Susan Mikkelsen, Khoa Manh Dinh, Thorsten Brodersen, Janna Nissen, Anne Karmisholt Grosen, Jens Kjærgaard Boldsen, Simon Mark Dahl Baunwall, Mie Topholm Bruun, Bitten Aagaard, Christina Mikkelsen, Christian Lodberg Hvas

“…Symptom phenotypes strongly correlate with sex, age, and bowel habits.…”
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Exercise-induced anxiety impairs local and systemic inflammatory response and glucose metabolism in C57BL/6J mice by I. Gálvez, M.C. Navarro, S. Torres-Piles, L. Martín-Cordero, M.D. Hinchado, E. Ortega

“…Phagocytic/microbicide activity, the expression of M1/M2 phenotype markers (CD11c, iNOS, CD206, ARG-1) and cytokines of the inflammatory response (MCP-1, IL-8, IL-6, TNF-α, TGF-β, IL-10) of peritoneal macrophages were determined via flow cytometry. …”
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Regulation of Shade Avoidance Under Low‐Blue‐Light by MTA in Soybean by Liya Zhang, Jun Liu, Jiaqi Chen, Yanyan Zhang, Chao Qin, Xiangguang Lyu, Zhuang Li, Ronghuan Ji, Bin Liu, Hongyu Li, Tao Zhao

“…It is found that the gmmtas mutants showed a substantial reduction of m6A levels, a dwarf phenotype, and a diminished sensitivity to LBL. Further investigation of the gmmtas mutants demonstrates that GmMTA regulates shade avoidance response by altering the expression of GmCRY1s, GmSPAs, and GmCOP1s, resulting in increased accumulation of GmSTFs that are known to suppress the shad avoidance response in response to LBL in soybean. …”
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Human proximal tubular epithelial cell interleukin-1 receptor signalling triggers G2/M arrest and cellular senescence during hypoxic kidney injury by Kurt T. K. Giuliani, Purba Nag, Benjamin C. Adams, Xiangju Wang, Seokchan Hong, Anca Grivei, Rebecca L. Johnston, Nicola Waddell, Kenneth K. C. Ho, Yilin Tian, Muhammad Ali Khan, Chang Seong Kim, Monica S. Y. Ng, Glenda Gobe, Jacobus P. J. Ungerer, Josephine M. Forbes, Helen G. Healy, Andrew J. Kassianos

“…Hypoxia+IL-1β-treated PTECs displayed signatures of cellular senescence, with reduced proliferation, G2/M cell cycle arrest, increased p21 expression, elevated senescence-associated β-galactosidase (SA-β-gal) activity and increased production of pro-inflammatory/fibrotic senescence-associated secretory phenotype (SASP) factors compared to normoxic conditions. …”
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Deficiency of FABP7 Triggers Premature Neural Differentiation in Idiopathic Normocephalic Autism Organoids by Xiao Han, Yuanlin He, Yuanhao Wang, Wenzhu Hu, Chu Chu, Lei Huang, Yuan Hong, Lu Han, Xu Zhang, Yao Gao, Yuan Lin, Hongxia Ma, Hongbing Shen, Xiaoyan Ke, Yan Liu, Zhibin Hu

“…ASD individuals with a shared clinical phenotype might contribute to revealing the molecular mechanism underlying ASD progression. …”
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Case report: Clinical characteristics and Genetical analysis of HSD11B2 in three Chinese children with apparent mineralocorticoid excess: a case series by Yuan Ding, Yuan Ding, Ming Cheng, Ming Cheng, Bingyan Cao, Bingyan Cao, Min Liu, Min Liu, Xuyun Hu, Xuyun Hu, Di Wu, Di Wu, Di Wu

“…Through our analysis, four novel variants of the HSD11B2 gene were identified, thereby enhancing the genotype-phenotype spectrum associated with AME. Early genetic testing in patients suspected of having AME is beneficial for facilitating prompt diagnosis and the implementation of standardized treatment protocols. …”
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SMARCA4 deficiency in small cell lung cancer: A case report and narrative review of the literature by Andreas M. Matthaiou, Ioannis Tomos, Nikoleta Bizymi, Ioannis Vamvakaris, Nektarios Anagnostopoulos, Aikaterini Papadopoulou, Kalliopi Angelou, Grigoris Stratakos, Adamantia Liapikou

“…This case indicates that SMARCA4-deficient small cell lung cancer may present with an aggressively deteriorating phenotype with poor outcomes for the patie …”
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Hypervirulent Klebsiella pneumoniae have better clinical outcomes than classical Klebsiella pneumoniae for lower respiratory tract infection patients by Xianxia Zhuo, Zichen Lei, Danni Pu, Yongli Wu, Jiankang Zhao, Bin Cao

“…Conclusions These findings suggest that capsule serotype is a more accurate factor for the prediction of the virulence phenotype, while hvKp have better clinical outcomes than cKp for LRTI patients. …”
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Possibility of alleviating dextran sulfate sodium-induced colitis in mice by modulate intestinal barrier function and gut microbiota with laminarin acetyl esters by Kit-Leong Cheong, Amanullah Sabir, Suresh Veeraperumal, Franck Quero, Rui Li, Qiaoli Zhao, Karsoon Tan, Saiyi Zhong, Udayakumar Veerabagu

“…Immunohistochemical analysis revealed a reduction in CD68 expression and an elevation in CD163 expression, indicating a shift toward an anti-inflammatory macrophage phenotype. These findings demonstrate that LA mitigates pro-inflammatory responses, enhances gut barrier integrity, and supports a healthy gut microbiota, underscoring its potential as a therapeutic agent for managing colitis and improving gut health.…”
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Comparison of Properties of Stem Cells Isolated from Adipose Tissue and Lipomas in Dogs by Takahiro Teshima, Akito Matsuoka, Maika Shiba, Kazuho Dairaku, Hirotaka Matsumoto, Ryohei Suzuki, Hidekazu Koyama

“…Some LDSCs isolated from canine subcutaneous lipomas were morphologically different from ADSCs and showed a rounded shape instead of fibroblast-like morphology. The phenotype of cell surface markers in LDSCs was similar to those in ADSCs, but CD29 and CD90 stem cell markers were more highly expressed compared with those of ADSCs. …”
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