March1 E3 Ubiquitin Ligase Modulates Features of Allergic Asthma in an Ovalbumin-Induced Mouse Model of Lung Inflammation by Osama A. Kishta, Antoine Sabourin, Leora Simon, Toby McGovern, Maxime Raymond, Tristan Galbas, Abdelilah Majdoubi, Satoshi Ishido, James G. Martin, Jacques Thibodeau

“…Herein, we used an acute model of allergy to compare the asthmatic phenotype of March1-deficient and -sufficient mice immunized with ovalbumin (OVA) and later challenged by intranasal instillation of OVA in the lungs. …”
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Designing host-associated microbiomes using the consumer/resource model by Germán Plata, Karthik Srinivasan, Madan Krishnamurthy, Lukas Herron, Purushottam Dixit

“…ABSTRACT A key step toward rational microbiome engineering is in silico sampling of realistic microbial communities that correspond to desired host phenotypes, and vice versa. This remains challenging due to a lack of generative models that simultaneously capture compositions of host-associated microbiomes and host phenotypes. …”
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Deep learning captures the effect of epistasis in multifactorial diseases by Vladislav Perelygin, Alexey Kamelin, Alexey Kamelin, Nikita Syzrantsev, Layal Shaheen, Layal Shaheen, Anna Kim, Nikolay Plotnikov, Anna Ilinskaya, Valery Ilinsky, Alexander Rakitko, Alexander Rakitko, Maria Poptsova

“…Then the higher performance of non-linear models over linear was confirmed on real genetic data for multifactorial phenotypes such as obesity, type 1 diabetes, and psoriasis. …”
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The APOE∗3-Leiden Heterozygous Glucokinase Knockout Mouse as Novel Translational Disease Model for Type 2 Diabetes, Dyslipidemia, and Diabetic Atherosclerosis by Marianne G. Pouwer, Suvi E. Heinonen, Margareta Behrendt, Anne-Christine Andréasson, Arianne van Koppen, Aswin L. Menke, Elsbet J. Pieterman, Anita M. van den Hoek, J. Wouter Jukema, Brendan Leighton, Ann-Cathrine Jönsson-Rylander, Hans M. G. Princen

“…E3L and E3L.GK+/− mice developed NASH with severe inflammation and fibrosis which, however, was not altered by introduction of the defective GK phenotype, whereas mild kidney pathology with tubular vacuolization was present in all three phenotypes. …”
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Yunvjian decoction attenuates lipopolysaccharide-induced acute lung injury by inhibiting NF-κB/NLRP3 pathway and pyroptosis by Fanxuan Zhang, Fang Wang, Lisha Zhao, Leqian Wang, Wenjing Li, Feihua Huang, Feihua Huang, Nani Wang, Nani Wang

“…YNJ-containing serum also decreased the secretion of tumor necrosis factor-α, IL-6, and IL-1β in LPS-stimulated RAW264.7 macrophages, and promoted macrophage polarization toward an M2 phenotype. A total of 23 absorbed components were identified in YNJ-containing serum. …”
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Circulating FGF21 is lower in South Asians compared with Europids with type 2 diabetes mellitus by Carlijn A Hoekx, Borja Martinez-Tellez, Maaike E Straat, Maurice B Bizino, Huub J van Eyk, Hildebrandus J Lamb, Johannes W A Smit, Ingrid M Jazet, Kimberly J Nahon, Laura G M Janssen, Patrick C N Rensen, Mariëtte R Boon

“…While South Asians are more prone to develop T2DM than Europids, the inflammatory phenotype of the South Asian population remains relatively unknown. …”
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BeatProfiler: Multimodal In Vitro Analysis of Cardiac Function Enables Machine Learning Classification of Diseases and Drugs by Youngbin Kim, Kunlun Wang, Roberta I. Lock, Trevor R. Nash, Sharon Fleischer, Bryan Z. Wang, Barry M. Fine, Gordana Vunjak-Novakovic

“…We then demonstrate that the data acquired by our automatic acquisition pipeline can be further harnessed for machine learning (ML) analysis to phenotype a disease model of restrictive cardiomyopathy and profile cardioactive drug functional response. …”
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Transcriptional regulation of miR528-PPO module by miR156 targeted SPLs orchestrates chilling response in banana by Xiangjin Kong, Kuan Peng, Youxia Shan, Ze Yun, Tamas Dalmay, Xuewu Duan, Yueming Jiang, Hongxia Qu, Hong Zhu

“…Transient overexpression of miR156c resulted in a more severe chilling phenotype by decreasing the expression of MaSPL4 and miR528. …”
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Case Report: The importance of genetic counseling for families with hyperinsulinism by Victoria R. Sanders, Victoria R. Sanders, Katherine Lord, Katherine Lord, Katherine Lord, Winnie M. Sigal, Winnie M. Sigal, Winnie M. Sigal, Heather McKnight, Heather McKnight, N. Scott Adzick, N. Scott Adzick, Lisa J. States, Lisa J. States, Tricia Bhatti, Tricia Bhatti, Diva D. De Leon, Diva D. De Leon, Diva D. De Leon

“…Congenital hyperinsulinism (HI) is the most common cause of persistent hypoglycemia in infancy. Genotype-phenotype correlations directly inform medical care for patients. …”
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Expression of miR-141 and Osteogenic Gene Dlx5, Msx2 and Runx2 in Osteogenic Differentiation of BMSCs in Ovariectomized Osteoporosis Model Mice by Zhenpu WEI, Wenming ZHANG, Pan SUN, Zhiqiang WANG, Yanping LIN

“…The morphology of the cells was observed and the cell phenotype was identified by flow cytometry. The osteogenic differentiation of BMSCs was observed by alkaline phosphatase staining, quantification and alizarin red staining on the 3rd, 8th and 13th day, respectively to observe the osteogenic ability of BMSCs. …”
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Cellular and immune landscape of chronic liver diseases: insights from immunophenotyping by Shaghayegh Soleimani, Shaghayegh Soleimani, Ozgur Albayrak, Kayra Somay, Hong Yang, Buket Yigit, Burge Ulukan, Dila Atak, Murat Akyildiz, Metehan Gursoy, Elif Demirtas, Adil Mardinoglu, Adil Mardinoglu, Atay Vural, Atay Vural, Murat Dayangac, Mujdat Zeybel

“…Differential expression analysis identified several genes associated with immune regulation, including downregulation of CD27 and upregulation of granzyme B in ARLD, consistent with a highly differentiated phenotype. LAG3 and PDCD1 were upregulated in peri-tumor samples. …”
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A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections by Amber De Bleeckere, Frits van Charante, Thibault Debord, Stien Vandendriessche, Michiel De Cock, Marte Verstraete, Fabien Lamret, Bram Lories, Jerina Boelens, Fany Reffuveille, Hans P. Steenackers, Tom Coenye

“…These data indicate that the in vitro SSF2 model could be a valuable addition to evaluate the antimicrobial susceptibility of biofilm-like aggregates in the context of PJI.IMPORTANCEInfections after joint replacement are rare but can lead to severe complications as they are difficult to treat due to the ability of pathogens to form surface-attached biofilms on the prosthesis as well as biofilm aggregates in the tissue and synovial fluid. This biofilm phenotype, combined with the microenvironment at the infection site, substantially increases antimicrobial tolerance. …”
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Multi-targeted nanogel drug delivery system alleviates neuroinflammation and promotes spinal cord injury repair by Penghui Wang, Zaifeng Chen, Ping Li, Abdullah Al Mamun, Shaoxia Ning, Jinjing Zhang, Chonghui Tang, Tianmiao Sun, Jian Xiao, Xiaojie Wei, Fenzan Wu

“…Crucially, our findings reveal that QLipTC@HDM induces M2 phenotype transformation in glial cells and in mice with SCI, thereby mitigating inflammation. …”
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Clinical features and genetic analysis of 15 Chinese children with dent disease by Qian Li, Zhenle Yang, Ruixian Zang, Suwen Liu, Lichun Yu, Jing Wang, Cong Wang, Xiaoyuan Wang, Shuzhen Sun

“…There was no significant difference in age or clinical phenotype among patients with different mutation types (p > 0.05). …”
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Characterization of Thirty Germplasms of Millet Pepper (<i>Capsicum frutescens</i> L.) in Terms of Fruit Morphology, Capsaicinoids, and Nutritional Components by Ruihao Zhang, Mengjuan Li, Junheng Lv, Pingping Li, Yunrong Mo, Xiang Zhang, Hong Cheng, Qiaoling Deng, Min Gui, Minghua Deng

“…PCA analysis using phenotypic data and physicochemical data showed that the classification results were different. …”
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Long-Term Analysis of Recurrence Beyond Milan Criteria Following Ablation of Solitary Early-Stage Hepatocellular Carcinoma &le;3 cm in Potentially Transplantable Patients: A Over 1... by Chen S, Qi H, Tan H, Cao F, Xie L, Huang T, Wu Y, Wen C, Wang Y, Shen L, Fan W

“…Elevated Platelet-CRP Score(PCS), integrating CRP and PLT, correlated significantly with an increased incidence of RBM and a more aggressive phenotype, characterized by vascular invasion or metastatic dissemination (P< 0.05). …”
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T cell immune senescence is associated with frailty and sarcopenia in lung transplant candidates by Joanna M. Schaenman, MD, PhD, Harry Pickering, PhD, Elaine F. Reed, PhD, Maura Rossetti, PhD, Benjamin Seligman, MD, S. Samuel Weigt, MD, Michael Shino, MD, David Sayah, MD, PhD, John Belperio, MD, Ashley Hu, MD, Ashley Prosper, MD, Kathleen Ruchalski, MD, Abbas Ardehali, MD, Reshma Biniwale, MD

“…Frailty was measured using the Fried Frailty Phenotype. Immune phenotyping was performed using multichannel flow cytometry of peripheral blood mononuclear cells (PBMC) in a total of 26 lung transplant candidates. …”
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Oligogenic effect is associated with the clinical heterogeneity of autosomal dominant deafness-15 by Jianyan Pan, Hua Teng, Fang Liu, Siyi Chen, Yaning Liu, Yanling Teng, Desheng Liang, Zhuo Li, Lingqian Wu

“…In this study, A four-generation Chinese family with 11 patients manifesting late-onset progressive non-syndromic hearing loss was recruited. The phenotype of hearing loss in this family showed a large variability in terms of onset age and progression speed. …”
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Comprehensive analysis of full genome sequence and Bd-milRNA/target mRNAs to discover the mechanism of hypovirulence in Botryosphaeria dothidea strains on pear infection with BdCV1... by Wangcheng Hu, Hui Luo, Yuekun Yang, Qiong Wang, Ni Hong, Guoping Wang, Aiming Wang, Liping Wang

“…BdCV1 had a greater effect on the phenotype, virulence, conidiomata, vertical and horizontal transmission ability, and mycelia cellular structure biological characteristics of B. dothidea strains than BdPV1 and virus-free strains. …”
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Lymphoid Progenitor Cells from Childhood Acute Lymphoblastic Leukemia Are Functionally Deficient and Express High Levels of the Transcriptional Repressor Gfi-1 by Jessica Purizaca, Adriana Contreras-Quiroz, Elisa Dorantes-Acosta, Eduardo Vadillo, Lourdes Arriaga-Pizano, Silvestre Fuentes-Figueroa, Horacio Villagomez-Barragán, Patricia Flores-Guzmán, Antonio Alvarado-Moreno, Hector Mayani, Isaura Meza, Rosaura Hernandez, Sara Huerta-Yepez, Rosana Pelayo

“…The aim of the present study was to investigate the ability of lymphoid progenitors from B-cell precursor ALL BM to proliferate and undergo multilineage differentiation. By phenotype analyses, in vitro proliferation assays, and controlled culture systems, the lymphoid differentiation potentials were evaluated in BM primitive populations from B-cell precursor ALL pediatric patients. …”
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