Paradoxical dominant negative activity of an immunodeficiency-associated activating PIK3R1 variant by Patsy R Tomlinson, Rachel G Knox, Olga Perisic, Helen Su, Gemma V Brierley, Roger L Williams, Robert K Semple

“…This attenuation of p110α signalling by a p110δ-activating PIK3R1 variant potentially explains co-incidence of gain-of-function and loss-of-function PIK3R1 phenotypes.…”
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Development of autonomic innervation at the venous pole of the heart: bridging the gap from mice to human by Fleur Zwanenburg, Thomas A. Bos, Arend D. J. Ten Harkel, Monique C. Haak, Nathan D. Hahurij, Robert E. Poelmann, Conny J. van Munsteren, Lambertus J. Wisse, Nico A. Blom, Marco C. DeRuiter, Monique R. M. Jongbloed

“…However, the timing of differentiation into sympathetic or parasympathetic phenotype was markedly distinct, as human autonomic markers emerged relatively later when related to major cardiogenesis. …”
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Elevated miR-221-3p inhibits epithelial–mesenchymal transition and biochemical recurrence of prostate cancer via targeting KPNA2: an evidence-based and knowledge-guided strategy by Dingchao Li, Jingang Jian, Manhong Shi, Zihao Chen, Anguo Zhao, Xuedong Wei, Yuhua Huang, Yalan Chen, Jianquan Hou, Yuxin Lin

“…Conclusions miR-221-3p down-regulation was a risk factor for PCa BRFS, and its over-expression could inhibit the malignant phenotype and EMT of PCa cells by directly targeting KPNA2. …”
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Nicotine promotes M2 macrophage polarization through α5-nAChR/SOX2/CSF-1 axis in lung adenocarcinoma by Guiyu Kang, Hui Song, Lei Bo, Qi Liu, Qiang Li, Jingtan Li, Pan Pan, Jingting Wang, Yanfei Jia, Haiji Sun, Xiaoli Ma

“…Correlations of α5-nAChR, SOX2 and M2 phenotype tumor-associated macrophages (TAMs) were validated in mouse LUAD models and clinical samples. α5-nAChR expression was connected to SOX2 expression, smoking and bad prognosis of LUAD among clinical samples. …”
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Construction and high-throughput screening of gradient nanowire coatings on titanium surface towards ameliorated osseointegration by Ruiyue Hang, Yuyu Zhao, Huanming Chen, Xiaomei Li, Runhua Yao, Yonghua Sun, Xiaohong Yao, Long Bai, Huaiyu Wang, Yong Han, Ruiqiang Hang

“…The results showed that macrophages (MФs) that mainly governed the initial inflammatory reaction exhibited a polarization tendency towards pro-healing M2 phenotype with decreased nanowire dimension due to nanomorphology-mediated focal adhesion formation and activation of its downstream signaling pathways (typically PI3K-Akt). …”
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RNA m6A involves in regulation of oxidative stress and apoptosis may via NF-kB pathway in cadmium-induced lung cells by Nan Zhang, Jie Yang, Yuan Zhao, Wenhong Li, Bo Zhao, Rongxian Li, Zuoshun He, Shiyan Gu

“…In present study, our data demonstrated that prolonged treatment of 1 μmol/L CdSO4 for 40 passages in bronchial epithelial cells (Beas-2B cells) resulted in the development of a malignant phenotype, which manifested as boosted proliferation, migration and invasion capacity as well as apoptosis reduction. …”
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Protective effect of adiponectin on oxidative stress-induced ovarian granulosa cell senescence in geese by Yan Zheng, Yunqiao Qiu, Ming Gao, Qianhui Wang, Lei Yu, Zhongzan Cao, Xinhong Luan

“…The results showed that ADPN could alleviate oxidative stress and cell cycle arrest in GCs, reduce the expression of the senescence-associated secretory phenotype (SASP)-related genes IL-6 and IL-8, regulate the metabolic capacity of GCs, reduce the accumulation of SA-β-gal, maintain telomere length, and alleviate the senescence of GCs induced by D-gal. …”
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Fasting activates optineurin-mediated mitophagy in chondrocytes to protect against osteoarthritis by Min-Na Zhang, Ran Duan, Gui-Hong Chen, Mei-Jun Chen, Chun-Gu Hong, Xin Wang, Zhi-Lin Pang, Chun-Yuan Chen, Hua-Feng Liu, Da Zhong, Hui Xie, Wen-Bao Hu, Zheng-Zhao Liu

“…The present study revealed a novel mechanism by which OPTN-mediated mitophagy influences chondrocytes and the OA phenotype in Optn-knockout mice, suggesting that OPTN-mediated mitophagy plays a crucial role in OA development and progression. …”
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A family with normal sperm motility carrying a sY86 deletion in AZFa region and partial deletion in AZFc region by Yuhong Zhao, Weiwei Zhi, Weiwei Zhi, Dongsheng Xiong, Dongsheng Xiong, Ningjing Li, Xinrong Du, Jiuzhi Zeng, Jiuzhi Zeng, Guohui Zhang, Guohui Zhang, Weixin Liu, Weixin Liu

“…Notably, although the son’s sperm motility parameters showed no significant abnormalities, there was a history of failed pregnancies for twice, with sperm exhibiting a high rate of head defect.DiscussionGiven the complexities of the reproductive phenotype following AZF region deletions, additional extended genetic testing is necessary when partial deletions in the AZF region are detected, thus providing more accurate predictions of the spermatogenesis in patient. …”
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Identification of potential druggable targets for endometriosis through Mendelian randomization analysis by Peng Chen, Xin Wei, Xiao-Ke Li, Yi-Hang Zhou, Yi-Hang Zhou, Qi-Fang Liu, Ling Ou-Yang

“…We applied a range of validation techniques, including reverse causality detection, phenotype scanning, Bayesian co-localization (BC) analysis, and external validations to substantiate our findings. …”
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3-Hydroxybutyrate alleviates septic liver injury by promoting macrophage polarization from M1 to M2 by Hu Jingjuan, Deng Fan, Sun Qishun, Min Yue, Feng Siyuan, Wu Ling, Chen Xiaofeng, Liu Kexuan

“…Peritoneal macrophage polarization (M1 and M2 phenotypes), liver injury, and inflammatory cytokine expression were analyzed. …”
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Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4 by Rongyu Shi, Ning Zhang, Han Li, Hu Zhong, Chengcheng Zhong, Wei Du, Xi Yang

“…Moreover, COL17A1 silencing in GEM-resistant PC cells or the incubation of COL17A1-decreased CAF with GEM-resistant PC cells could suppress GEM resistance and cell oncogenic phenotype progression. Mechanistically, COL17A1 interacted with ACTN4 protein, and the anticancer effects mediated by COL17A1-decreased CAFs in resistant PC cells were reversed by ACTN4 overexpression. …”
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Lipids and C-reactive protein predict anhedonia and reward circuit functional connectivity responses to anti-cytokine and dopaminergic therapies in patients with depression by Aditya Singh, Mandakh Bekhbat, David R. Goldsmith, Ngoc-Anh Le, Evanthia C. Wommack, Zhihao Li, Ebrahim Haroon, Jennifer C. Felger

“…Increased inflammation and associated metabolic disturbances have been shown to affect neurotransmitters and brain circuits, contributing to an immunometabolic phenotype of anhedonic depression. To extend our previous findings on relationships between plasma lipids and antidepressant response to anti-cytokine therapy, we explored in secondary analyses whether lipid-related biomarkers similarly predicted change in anhedonia or functional connectivity (FC) in dopamine-rich corticostriatal reward circuitry in medically-stable, depressed patients with a range of inflammation levels (indexed by plasma C-reactive protein [CRP]) who were administered inflammation-targeted therapies. …”
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Cell Morphology on Poly(methyl methacrylate) Microstructures as Function of Surface Energy by Matthias Katschnig, Boris Maroh, Natascha Andraschek, Sandra Schlögl, Ulrike Zefferer, Elisabeth Bock, Gerd Leitinger, Christa Trattnig, Maria Kaufmann, Werner Balika, Clemens Holzer, Ute Schäfer, Silke Patz

“…Here we evaluate the hypothesis that surface energy plays a decisive role in substrate-mediated modulation of cell phenotype by evaluation of cell behaviour on synthetic microstructures exhibiting pronounced differences in surface energy. …”
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Unraveling the pathogenesis of Barrett’s esophagus and esophageal adenocarcinoma: the “omics” era by Alberto Barchi, Giuseppe Dell’Anna, Giuseppe Dell’Anna, Luca Massimino, Luca Massimino, Francesco Vito Mandarino, Edoardo Vespa, Edi Viale, Sandro Passaretti, Vito Annese, Vito Annese, Alberto Malesci, Alberto Malesci, Silvio Danese, Silvio Danese, Federica Ungaro

“…Barrett’s esophagus (BE) represents a pre-cancerous condition that is characterized by the metaplastic conversion of the squamous esophageal epithelium to a columnar intestinal-like phenotype. BE is the consequence of chronic reflux disease and has a potential progression burden to esophageal adenocarcinoma (EAC). …”
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Psychological stress disturbs bone metabolism via miR-335-3p/Fos signaling in osteoclast by Jiayao Zhang, Juan Li, Jiehong Huang, Xuerui Xiang, Ruoyu Li, Yun Zhai, Shuxian Lin, Weicai Liu

“…Our result found that these CUMS mice exhibited osteoporosis phenotype that is mainly attributed to the abnormal activities of osteoclasts. …”
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Acute Bronchiolitis To Pediatric Inpatient Clinic In Patients Under 2 Years Old, Installed with Diagnosis Investigation of the Relationship of Bronchiolitis and Asthma by Selin Kuzucu, İpek Türktaş, Tuğba Bedir Demirdağ, Meltem Polat, Hasan Tezer, Anıl Tapısız, Nazmi Mutlu Karakaş, Ayşe Tana Aslan, Hakan Tüzün

“…Objective: Previously considered a uniform disease, bronchiolitis has been revealed through research as heterogeneous, displaying varied phenotypes and clinical-histopathological differences among patients. …”
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MicroRNA-451a is a candidate biomarker and therapeutic target for major depressive disorder by Yan Chen, Yun Liu, Ming Xiao, Chun Wang, Panpan Hu, Qiuchen Cao, Hu Feng, Jingfan Xu, Weixi Feng, Huaiqing Sun, Huachen Ding, Junying Gao

“…Overexpressing miR-451a in the mPFC reversed the loss of dendritic spines and the depression-like phenotype of CRS mice. Mechanistically, miR-451a could inhibit CRS-induced corticotropin-releasing factor receptor 1 expression via targeting transcription factor 2, subsequently protecting dendritic spine plasticity.Conclusions Together, these results highlighted miR-451a as a candidate biomarker and therapeutic target for MDD.…”
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Modulation of Acid Sphingomyelinase in Melanoma Reprogrammes the Tumour Immune Microenvironment by Emma Assi, Davide Cervia, Laura Bizzozero, Annalisa Capobianco, Sarah Pambianco, Federica Morisi, Clara De Palma, Claudia Moscheni, Paolo Pellegrino, Emilio Clementi, Cristiana Perrotta

“…Since acid sphingomyelinase (A-SMase) downregulation in melanoma was shown to determine a malignant phenotype, we aimed here to elucidate the role of A-SMase in the regulation of tumour immunogenic microenvironment using in vivo melanoma models in which A-SMase was either downregulated or maintained at constitutively high levels. …”
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Ruxolitinib synergizes with regulatory T cells to improve inflammation but has no added benefits in decreasing albuminuria in SLE by Mi-Ae Lyu, Ximing Tang, Maria Gabriela Raso, Meixian Huang, Ke Zeng, Tara Sadeghi, Christopher R. Flowers, Simrit Parmar

“…We hypothesize that the addition of ruxolitinib to UCB-Tregs may improve SLE outcomes.MethodsIn vitro cell suppression, phenotype change, IL-10 secretion, and cytokine levels in coculture supernatants were determined to quantify the impact of adding ruxolitinib to UCB-Tregs. …”
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