Coinfection of severe acute respiratory syndrome coronavirus 2 and polyomavirus in a patient with coronavirus disease 2019 detected by enrichment next-generation sequencing: a case... by Gunadi, Esensi Tarian Geometri, Pramana Adhityo, Ali Zainal Abidin, Marcellus, Dyah Ayu Puspitarani, Fadila Dyah Trie Utami, Kristy Iskandar, Hendra Wibawa, Mohamad Saifudin Hakim

“…Whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 was performed using the Nextera DNA Flex for Enrichment using the Respiratory Virus Oligos Panel, Illumina MiSeq instruments, and Illumina MiSeq reagent v3 150 cycles (2 × 75 cycles). …”
Get full text

Which Measure of Stone Burden is the Best Predictor of Interventional Outcomes in Urolithiasis: A Systematic Review and Meta-analysis by the YAU Urolithiasis Working Group and EAU... by Robert Geraghty, Amelia Pietropaolo, Lazaros Tzelves, Riccardo Lombardo, Helene Jung, Andreas Neisius, Ales Petrik, Bhaskar K. Somani, Niall F. Davis, Giovanni Gambaro, Romain Boissier, Andreas Skolarikos, Thomas Tailly

“…However, recent studies have shown that multidimensional measures provide better prediction of outcomes. Methods: We performed a systematic review and meta-analysis of the prognostic accuracy of measures of stone size (PROSPERO reference CRD42022346967). …”
Get full text

Cyanoacrylate glue in breast surgery: the GLUBREAST Trial by Emanuela Esposito, Claudio Siani, Ivana Donzelli, Anna Crispo, Sergio Coluccia, Piergiacomo Di Gennaro, Assunta Luongo, Franca Avino, Alfredo Fucito, Ugo Marone, Maria Teresa Melucci, Ruggero Saponara, Raimondo di Giacomo

“…Stratified models by visits were performed.ResultsThe trial ended in June 2022. Older age was associated with a higher volume of seroma drained per day (β 0.30; 95% CI: 0.00–0.60). …”
Get full text

Assessment of Antibiotic Resistance Among Isolates of <i>Klebsiella</i> spp. and <i>Raoultella</i> spp. in Wildlife and Their Environment from Portugal: A Positive Epidemiologic Ou... by Carolina Sabença, Rani de la Rivière, Paulo Barros, João Alexandre Cabral, Roberto Sargo, Luís Sousa, Maria de Lurdes Enes Dapkevicius, Filipe Silva, Filipa Lopes, Ana Carolina Abrantes, Madalena Vieira-Pinto, Manuela Caniça, Gilberto Igrejas, Carmen Torres, Patrícia Poeta

“…<i>Klebsiella</i> and <i>Raoultella</i> genera isolates were recovered from fecal samples of wild animals and environmental samples using media without antibiotic supplementation. Antibiograms were performed for 15 antibiotics to determine the phenotypic resistance profile in these isolates. …”
Get full text

Assessment of myocardial deformation by CMR tissue tracking reveals left ventricular subclinical myocardial dysfunction in patients with gynecologic cancer undergoing chemotherapy by Kai Tao, Kai Tao, Lu Ye, Yan-Jia-Ni Xu, Yan-Jia-Ni Xu, Meng-Xi Yang, Ru-Tie Yin, Qing-Li Li, Xiao-Juan Lin, Ke-Min Li, Liang Song, Yu Ma, Lan Zhong, Ying Hu, Hua-Yan Xu, Dan-Qing Wang, Ying-Kun Guo

“…This study aimed to assess left ventricular (LV) myocardial deformation in patients with gynecologic cancer undergoing chemotherapy and to investigate the association between myocardial deformation and chemotherapy factors.MethodsCardiac magnetic resonance (CMR) was performed to assess LV deformation parameters using CMR tissue tracking based on cine images. …”
Get full text

Construction and Pathogenicity Analysis of a GFPuv-labeled Peanut Ralstonia solanacearum Strain by Jinling HUANG, Yushuang WU, Huiquan TANG, Minyi HUANG, Ruixue YANG, Qiang WANG, Yong YANG, Xiaodan TAN

“…Growth rate and pathogenicity determination were performed to evaluate the functional activity of the GFPuv-labeled strain.…”
Get full text

Evidence construction of Silibinin capsules against alcoholic liver disease based on a meta-analysis and systematic review by Yan Wang, Yan Wang, Wenmin Yang, Wenmin Yang, Yuan Yang, Yuan Yang, Xingning Liu, Xingning Liu, Lanfen Peng, Lanfen Peng, Qi Huang, Qi Huang, Qi Huang, Kongli Fan, Kongli Fan, Rui Hu, Rui Hu, Jinyu Yi, Jinyu Yi, Xin Zhong, Xin Zhong, Jing Li, Jialing Sun, Xiaozhou Zhou, Xiaozhou Zhou

“…Primary outcomes included liver function indicators such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), total bilirubin (TBIL), lipid indicators including triglycerides (TG) and total cholesterol (TC), coagulation indicators including prothrombin time (PT), liver fibrosis indicator (PC-III), and Effective Rate. Analysis was performed using Review Manager 5.4.1 and STATA 14.0.ResultsIn 15 RCTs involving 1,221 patients, compared to the non-Silibinin group, Silibinin capsules showed significant efficacy in terms of liver function, lipid levels, and effective rate in patients with ALD. …”
Get full text

Interactions of the emerging fungus Candida auris with Acanthamoeba castellanii reveal phenotypic changes with direct implications on the response to stress and virulence by Michele Ramos Valente, Lucas Martins Alcântara, Deborah Santos Cintra, Susana Ruiz Mendoza, Elisa Gonçalves Medeiros, Kamilla Xavier Gomes, Leandro Honorato, Marcos de Abreu Almeida, Carmen Baur Vieira, Joshua Daniel Nosanchuk, Diana Bridon da Graça Sgarbi, Marcia Ribeiro Pinto, Leonardo Nimrichter, Allan Jefferson Guimarães

“…It may be essential to understand the various interactions C. auris could perform in the environment, directly impacting the outcome of human infections under the One Health approach.…”
Get full text

Sherlock 3CGtip confirmation system for placement of peripherally inserted central catheters and related nursing countermeasures in a patient with double superior vena cava (1例双上腔静... by SONG Lanna (宋兰娜), WANG Yuan (王媛), WANG Wenxuan (王文萱), WANG Yu (王钰), ZHANG Na (张娜)

“…The X-ray positioning was performed to confirm that the tip of the catheter was located in the left superior vena cava. …”
Get full text

Clonal hematopoiesis of indeterminate potential is a risk factor of gastric cancer: A Prospective Cohort in UK Biobank study by Zhihui Xi, Huolun Feng, Kunling Chen, Xin Guo, Dandan Zhu, Jiabin Zheng, Yong Li

“…Exposures: Whole-exome sequencing was performed on blood samples collected at baseline. A CHIP status was identified based on the mutations on 43 CHIP-related genes. …”
Get full text

Inverse association between obesity and suicidal death risk by Joonyub Lee, Seung-Hwan Lee, Mee-Kyoung Kim, Hyuk-Sang Kwon, Jae-Seung Yun, Yeoree Yang, Kun-Ho Yoon, Jae-Hyoung Cho, Chi-Un Pae, Kyungdo Han, Jang Won Son

“…Multivariate Cox proportional hazard regression analysis was performed to estimate the risk of suicidal death with respect to BMI categories after adjusting for potential confounders (age, sex, income, diabetes, hypertension, dyslipidemia, smoking, drinking, exercise, self-abuse, waist circumference, schizophrenia, bipolar disorder, eating disorder, cancer, anxiety, and substance use disorder). …”
Get full text

Intestinal butyric acid-mediated disruption of gut hormone secretion and lipid metabolism in vasopressin receptor-deficient mice by Kazuki Harada, Eiji Wada, Yuri Osuga, Kie Shimizu, Reiko Uenoyama, Masami Yokota Hirai, Fumihiko Maekawa, Masao Miyazaki, Yukiko K. Hayashi, Kazuaki Nakamura, Takashi Tsuboi

“…Methods: We used the mouse GLP-1-secreting cell line, GLUTag, and performed live cell imaging to examine the contribution of V1a and V1b vasopressin receptors (V1aR and V1bR, respectively) to GLP-1 secretion. …”
Get full text

ALDH1L2 drives HCC progression through TAM polarization by Jiajun Li, Chi Zhang, Qingqing Zhou, Qinqin Long, Jiayi Chen, Lili Meng, Wei Tian, Yue Yang, Chao Ge, Yuting Su, Xi-Dai Long, Jun Wu, Hua Tian

“…A series of in vitro and in vivo assays were performed to explore the role and molecular mechanism of ALDH1L2 in HCC progression. …”
Get full text

Evaluation of health care providers’ ability to identify patient-ventilator triggering asynchrony in intensive care unit: a translational observational study in China by Shengjun Liu, Zhangyi Zhao, Xiangyu Chen, Yi Chi, Siyi Yuan, Fuhong Cai, Zhangwei Song, Yue Ma, Huaiwu He, Longxiang Su, Yun Long

“…Professionals who published more than 2 articles in the field of critical respiratory performed better on auto-triggers [41.7% vs. 15.9% (p = 0.001)] and reverse triggers [38.9% vs. 19.2% (p = 0.018)]. …”
Get full text

PINK1 modulates Prdx2 to reduce lipotoxicity‐induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction by Hao Zhang, Tianyu Xu, Xiyuan Mei, Qiming Zhao, Qiling Yang, Xianghui Zeng, Zhuang Ma, Haobin Zhou, Qingchun Zeng, Dingli Xu, Hao Ren

“…Immunoprecipitation, western blotting and immunofluorescence analysis were performed to elucidate the molecular mechanisms involved. …”
Get full text

The effect of renal function on the clinical outcomes and management of patients hospitalized with hyperglycemic crises by Sumaya N. Almohareb, Sumaya N. Almohareb, Sumaya N. Almohareb, Norah Aljammaz, Nada Yousif, Mayar Sunbul, Raghad Alsemary, Lama Alkhathran, Mohammed Aldhaeefi, Omar A. Almohammed, Abdulrahman I. Alshaya, Abdulrahman I. Alshaya, Abdulrahman I. Alshaya

“…Statistical analyses were performed using SAS® software.ResultsOut of 319 screened patients, 183 patients met the inclusion criteria. …”
Get full text

A patient-derived HCC spheroid system to model the tumor microenvironment and treatment response by Emilie Crouchet, Nuno Almeida, Sarah C. Durand, Marie Parnot, Marine A. Oudot, Fabio Giannone, Cloé Gadenne, Natascha Roehlen, Antonio Saviano, Emanuele Felli, Patrick Pessaux, Hong Tuan Duong, Hideki Ohdan, Hiroshi Aikata, Kazuaki Chayama, Thomas F. Baumert, Catherine Schuster

“…Characterization of the tumor spheroid cell populations was performed by flow cytometry, immunohistochemistry (IHC), and functional assays. …”
Get full text

The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells by Weronika Zarychta-Wiśniewska, Anna Burdzińska, Katarzyna Zielniok, Marta Koblowska, Kamila Gala, Piotr Pędzisz, Roksana Iwanicka- Nowicka, Anna Fogtman, Aleksandra Aksamit, Agnieszka Kulesza, Aleksandra Zołocińska, Leszek Pączek

“…The microarray analysis was performed on RNA isolated from hASC and hBM-MSC A cells. …”
Get full text

Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…The immunofluorescence staining, flow cytometry, qPCR, single-cell contraction, intracellular calcium transients, patch-clamp, dual luciferase reporter assay and western blot were performed for the study. Results Modeling TTC with high doses of epinephrine (Epi) treatment in hiPSC-CMs shows suppression of depolarization velocity (Vmax), prolongation of action potential duration (APD), and induction of arrhythmic events. …”
Get full text

Target trial emulation to evaluate the effect of immune-related adverse events on outcomes in metastatic urothelial cancer by Renate Pichler, Josef Fritz, Sarah Maier, Melanie R. Hassler, Johanna Krauter, David D`Andrea, Ekaterina Laukhtina, Kilian Gust, Keiichiro Mori, Karl H. Tully, Dora Niedersuess-Beke, Lea Korber, Jasmin Alija Spiegelberg, Thomas Bauernhofer, José D. Subiela, Roman Mayr, Andreas Kronbichler, Marco Moschini, Jeremy Teoh, Benjamin Pradere, Shahrokh F. Shariat, Hanno Ulmer, Laura S. Mertens, European Association of Urology–Young Academic Urologists (EAU-YAU): Urothelial Carcinoma Working Group

“…Methods TTE was contrasted to adjusted naïve and time-updated Cox models. We performed a multi-institutional retrospective study involving mUC patients under ICI. …”
Get full text