Fibroblast growth factor receptor 3 mutation promotes HSPB6-mediated cuproptosis in hypochondroplasia by impairing chondrocyte autophagy by Jing Chen, Dan He, Chengrun Yuan, Na Li, Baohong Shi, Conway Niu, Jiangfei Yang, Liangkai Zheng, Lin Che, Ren Xu

“…Methods: We generated an Fgfr3 (V376D) mutation mouse model using CRISPR/Cas9 technology and performed proteomic analyses to investigate the molecular mechanisms and potential therapeutic targets of HCH. …”
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Clinical characteristics and outcomes of patients with chondroblastoma undergoing surgery with various adjuvant procedures: a retrospective study of 59 cases by Toru Hirozane, Tetsuya Sekita, Eisuke Kobayashi, Tomoaki Mori, Naofumi Asano, Toru Udaka, Takashi Tajima, Rumi Nakagawa, Kazutaka Kikuta, Akira Yoshiyama, Hideo Morioka, Itsuo Watanabe, Ukei Anazawa, Michiro Susa, Keisuke Horiuchi, Yoshihisa Suzuki, Takeshi Morii, Robert Nakayama

“…The most common lesion site was the epiphyses of long bones (42 cases, 71%), including the femur, tibia, and humerus. Curettage was performed in 57 cases; 2 cases with an iliac lesion underwent resection. …”
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The impact of pre-transplant ventricular assist device support in pediatric patients with end-stage heart failure on the outcomes of heart transplantation—“a single center experien... by L. Lily Rosenthal, L. Lily Rosenthal, L. Lily Rosenthal, Carola Grinninger, Sarah Marie Ulrich, Robert Dalla Pozza, Nikolaus A. Haas, Paolo Brenner, Michael Schmoeckel, Sebastian Michel, Sebastian Michel, Sebastian Michel, Christian Hagl, Christian Hagl, Jürgen Hörer, Jürgen Hörer, Jürgen Hörer

“…In light of the limited availability of donor organs, particularly in Europe, the number of children requiring ventricular assist device support is rising at an unavoidable rate.MethodsWe performed a retrospective cohort study of patients who underwent a single and primary pediatric heart transplantation. …”
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Prevalence and clinical profile of comorbidity among newly diagnosed pulmonary tuberculosis patients: a multi-center observational study in eastern China by Wei Wang, Wei Wang, Xiaomeng Wang, Xiaomeng Wang, Songhua Chen, Jun Li, Qinglin Cheng, Yu Zhang, Qian Wu, Qian Wu, Kui Liu, Kui Liu, Xuli Jiang, Bin Chen, Bin Chen

“…Univariate and multivariate logistic regression analyzes were performed to identify the effects of comorbidities on the clinical features of the participants.ResultsOf the 8,421 total participants, 27.6% reported cavities in the chest radiography results, 41.9% were Mycobacterium tuberculosis-positive in the etiology test results, and 38.7% (3,258/8,421) had at least one type of comorbidity. …”
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Association between Loa loa microfilaremia and anatomical hyposplenia in a rural area of the Republic of Congo: a population-based cross-sectional study by Charlotte Boullé, Elodie Lebredonchel, Jérémy T. Campillo, Valentin Dupasquier, Marlhand C. Hemilembolo, Sébastien D. S. Pion, Jean Claude Djontu, Ludovic Rancé, Philippe Souteyrand, François Missamou, Michel Boussinesq, Francine Ntoumi, Cédric B. Chesnais

“…Centralized ultrasonographic examination was performed. The primary outcomes included SV, splenomegaly (cranio-caudal-distance ≥ 13 cm), and anatomical hyposplenia (AH) (SV ≤ 80, ≤ 110 or ≤ 150 cm3). …”
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Identification of biomarkers for the diagnosis of type 2 diabetes mellitus with metabolic associated fatty liver disease by bioinformatics analysis and experimental validation by Guiling Wu, Guiling Wu, Sihui Wu, Sihui Wu, Tian Xiong, Tian Xiong, Tian Xiong, You Yao, You Yao, Yu Qiu, Yu Qiu, Yu Qiu, Liheng Meng, Cuihong Chen, Xi Yang, Xi Yang, Xi Yang, Xinghuan Liang, Yingfen Qin

“…Therefore, we aimed to identify potential biomarkers for diagnosing both conditions.MethodsWe performed differential expression analysis and weighted gene correlation network analysis (WGCNA) on publicly available data on the two diseases in the Gene Expression Omnibus database to find genes related to both conditions. …”
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Logistic Regression Model in a Machine Learning Application to Predict Elderly Kidney Transplant Recipients with Worse Renal Function One Year after Kidney Transplant: Elderly KTbo... by Ubiracé Fernando Elihimas Júnior, Jamila Pinho Couto, Wallace Pereira, Michel Pompeu Barros de Oliveira Sá, Eduardo Eriko Tenório de França, Filipe Carrilho Aguiar, Diogo Buarque Cordeiro Cabral, Saulo Barbosa Vasconcelos Alencar, Saulo José da Costa Feitosa, Thais Oliveira Claizoni dos Santos, Helen Conceição dos Santos Elihimas, Emilly Pereira Alves, Marcio José de Carvalho Lima, Frederico Castelo Branco Cavalcanti, Paulo Adriano Schwingel

“…Unicentric retrospective analysis of a cohort was performed with individuals aged ≥60 and <60 years old. …”
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High-throughput methylation sequencing reveals novel biomarkers for the early detection of renal cell carcinoma by Wenhao Guo, Weiwu Chen, Jie Zhang, Mingzhe Li, Hongyuan Huang, Qian Wang, Xiaoyi Fei, Jian Huang, Tongning Zheng, Haobo Fan, Yunfei Wang, Hongcang Gu, Guoqing Ding, Yicheng Chen

“…Methods High-throughput methylation sequencing was performed on peripheral blood samples obtained from 49 primarily Stage I RCC patients and 44 healthy controls. …”
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Impact of Sex-Related Differences in Infrarenal Aortic Neck Morphology on Outcomes of Endovascular Aneurysm Repair for Similar-Sized Aortic Aneurysm by Ombretta Martinelli, Antonio Marzano, Maria Irene Bellini, Roberto Gattuso, Luca Di Marzo, Valeria Gonta, Jihad Jabbour, Wassim Mansour, Simone Cuozzo

“…<b>Methods</b>: This study performed a retrospective analysis of patients electively treated by EVAR for infrarenal AAA between January 2019 and December 2023. …”
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Plasmodium falciparum transcription factor AP2-06B is mutated at high frequency in Southeast Asia but does not associate with drug resistance by Qiyang Shi, Changhong Wang, Wenluan Yang, Xiaoqin Ma, Jianxia Tang, Jiayao Zhang, Guoding Zhu, Yinlong Wang, Yaobao Liu, Xiaoqin He

“…One mutation, K3124R, was located in a PfAP2-06B AP2 domain.MethodsTo investigate transcriptional regulation by PfAP2-06B, ChIP-seq assays were performed on 3D7/PfAP2-06B-GFP schizonts using antibodies against GFP. …”
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Pragmatic algorithm for visual assessment of 4-Repeat tauopathies in [18F]PI-2620 PET Scans by Theresa Bauer, Matthias Brendel, Mirlind Zaganjori, Alexander M. Bernhardt, Alexander Jäck, Sophia Stöcklein, Maximilian Scheifele, Johannes Levin, Thilo van Eimeren, Alexander Drzezga, Osama Sabri, Henryk Barthel, Robert Perneczky, Günter Höglinger, Nicolai Franzmeier, Johannes Gnörich

“…A regional analysis was performed using the cortex, basal ganglia, midbrain, and dentate nucleus. …”
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Integration analysis of microRNAs as potential biomarkers in early-stage lung adenocarcinoma: the diagnostic and therapeutic significance of miR-183-3p by Guodong Huang, Yuxia Liu, Lisha Li, Bing Li, Ting Jiang, Yufeng Cao, Xiaoping Yang, Xinning Liu, Honglin Qu, Shitao Li, Xin Zheng

“…Gene-specific experimental validation was performed using qRT-PCR, western blotting and luciferase reporter assays.ResultsWe identified 36 differentially expressed miRNAs between LUAD tissues and matched paracancerous tissues. …”
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The walnut tree as a source of progesterone for reproductive control in goats by P. Chemineau, A.L. Lainé, D. Gennetay, C. Porte, D. Chesneau, C. Laclie, G. Goudet, M. Meunier, M. Delmas, M.L. Greil, P. Liere, A. Pianos, A. Bernard, E. Dirlewanger, J.A. Delgadillo, M. Keller

“…To determine their dietary acceptance and their efficacy in terms of P4 blood plasma concentration, three trials in ovariectomised goats and four trials in ovary-intact goats were performed (N = 83). The distribution of 600 g of pellets per day per ovary-intact goat over 3 days, 6 and 4 days before the introduction of males in April allowed us to achieve our objective of a significant increase of P4 plasma concentration to ∼1.5 ng/mL measured by EIA from 24 to 72 h after the first distribution in the walnut pellet group (n = 13). …”
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Co-benefits of nature-based solutions: A health impact assessment of the Barcelona Green Corridor (Eixos Verds) plan by Tamara Iungman, Sergi Ventura Caballé, Ricard Segura-Barrero, Marta Cirach, Natalie Mueller, Carolyn Daher, Gara Villalba, Evelise Pereira Barboza, Mark Nieuwenhuijsen

“…Preventable mortality at the census tract level for adult residents was estimated, and to address uncertainties, we performed Monte Carlo iterations. We computed the percentage increase in GS (land use) and tree cover and converted them into Normalized Difference Vegetation Index (NDVI) to conduct the HIA, employing a Generalized Additive Model (GAM). …”
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Low-activity [18F]-somatostatin receptor (SSTR) imaging using [18F]SiTATE on a long axial field-of-view PET/CT scanner by Nils F. Trautwein, Eduardo Calderón, Pia M. Linder, Gerald Reischl, Philippe Driessen, Wenhong Lan, Andreas S. Brendlin, Thorben Groß, Helmut Dittmann, Martina Hinterleitner, Christian la Fougère, Fabian P. Schmidt, Lena S. Kiefer

“…A comparison of image reconstruction in high sensitivity (HS) and ultra-high sensitivity mode (UHS) mode was performed. Subjective image quality, noise and lesion detectability of n = 122 lesions were rated using a 5-point Likert scale. …”
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Resting-state functional brain connectivity in female adolescents with first-onset anorexia nervosa by Katrien F.M. Bracké, Laura Monteiro Rente Dias, Marisha N. Meijer, Cathelijne P.M. Steegers, Laurinde F. den Heijer, Tess van der Harst, Marjolein H.G. Dremmen, Meike W. Vernooij, Gwen C. Dieleman, Tonya White

“…To account for the severity of underweight, separate analyses were performed to investigate differences in RS-FC between underweight AN participants and TD adolescents, as well as between underweight (n = 30) and weight-restored AN (n = 26) participants. …”
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IL-6/GATA2/SERPINE1 pathway is implicated in regulating cellular senescence after acute kidney injury by Hongshuang Su, Xiaoxi Lin, Ayinuer Paredong, Ayinuer Paredong, Congcong Yao, Yan Zhang, Mengke Geng, Yuqian Guan, Lichao Gong, Feng Jiang, Qi Lv, Songtao Shou, Heng Jin

“…RNA sequencing analysis was performed on cells treated with myoglobin and tocilizumab. …”
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Detection of cervical precancerous lesions and cancer by small-scale RT-qPCR analysis of oppositely deregulated mRNAs pairs in cytological smears by Anastasia A. Artyukh, Mikhail K. Ivanov, Mikhail K. Ivanov, Sergei E. Titov, Sergei E. Titov, Victoria V. Dzyubenko, Sergey E. Krasilnikov, Sergey E. Krasilnikov, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Nikita A. Afanasev, Anastasia V. Malek, Sergei A. Glushkov, Eduard F. Agletdinov

“…Additionally, in the same specimens, the HPV genotyping, viral load assessment, diagnosis of cervicovaginal microbiome imbalance and profiling of some relevant mRNAs and miRNAs were performed by qPCR-based methods.ResultsThe best classifiers were obtained with pairs of mRNAs whose expression changes in opposite directions during lesion progression. …”
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A conserved element in the first intron of Cd4 has a lineage specific, TCR signal-responsive, canonical enhancer function that matches the timing of cell surface CD4 upregulation r... by Gregory A. Swan, Gregory A. Swan, Chika Fujii, Mia E. Guzynski, Sheridan M. Page, Isabelle V. Meyers, Yordan P. Penev, Sejiro Littleton, Sejiro Littleton, Adinda Azzahra, Christine Richardson, Sophia D. Sarafova, Sophia D. Sarafova

“…In this study, we investigate whether a conserved region, here called NCE, that is proximal to the Cd4 silencer and contains E4m has the required developmental-stage-specific canonical enhancer function and TCR responsiveness to mediate the CD4 upregulation required to prevent lineage errors.MethodsTo investigate the role of NCE, transient transfection of reporter plasmids was performed in thymoma cell lines arrested at the double-positive (DP, CD4+CD8+) and intermediate (INT, CD4+CD8lo) stages of development. …”
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MiR-216a-3p inhibits the cytotoxicity of primary natural killer cells by Rowan Abdelbary, Rowan Abdelbary, Manon Ragheb, Manon Ragheb, Shereen A. El Sobky, Nagwa El-Badri, Nourhan Aboud, Ahmed Tawheed, Asmaa Gomaa, Mona Zidan, Ramy K. Aziz, Abd Elrahman Abouzid, Radwa Ayman Salah, Mohamed El-Kassas, Imam Waked, Ahmed Moustafa, Ahmed Moustafa, Injie Omar Fawzy, Nada El-Ekiaby, Ahmed Ihab Abdelaziz

“…Furthermore, we induced the expression of miR-216a-3p in NK cells isolated from healthy controls, followed by measuring the release of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), perforins (PRF) and granzyme B (GrB) using ELISA as well as NK cells cytolytic activity against Huh7 cells using lactate dehydrogenase (LDH) cytotoxicity assay. After that, we performed an in silico analysis to understand the mechanistic regulation imposed by miR-216a-3p on NK cells to study its impact on one of its potential downstream targets.ResultsOur results have indicated that miR-216a-3p has higher expression in NK cells of patients with HCC, and simulating this elevated expression pattern via forcing miR-216a-3p expression in normal NK cells has negatively impacted the release of TNF- α, IFN- γ, GrB, and PRF. …”
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