Image-based robotic (ROSA® knee system) total knee arthroplasty with inverse kinematic alignment compared to conventional total knee arthroplasty by Henriëtte M. Eijking, Isobel M. Dorling, Emil H. van Haaren, Roel Hendrickx, Thijs Nijenhuis, Martijn G.M. Schotanus, Lee Bouwman, Jasper Most, Bert Boonen

“…In the present study, robotic-assisted TKA will be performed to pursue inverse kinematic alignment (iKA) within predefined boundaries, focusing on restoring native tibia joint line. …”
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Concurrent Panniculectomy With Abdominal Wall Reconstruction: A Propensity-scored Matched Study of Quality Improvement Outcomes by Alexis M. Holland, MD, William R. Lorenz, MD, Matthew N. Marturano, MD, Rose K. Hollingsworth, BA, Gregory T. Scarola, MS, Brittany S. Mead, MD, B. Todd Heniford, MD, Vedra A. Augenstein, MD

“…A 1:1 propensity-scored matching was performed based on age, tobacco use, body mass index (BMI), American Society of Anesthesiologists (ASA) score, wound class, and defect size. …”
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Integration of single-cell transcriptomics and bulk transcriptomics to explore prognostic and immunotherapeutic characteristics of nucleotide metabolism in lung adenocarcinoma by Kai Zhang, Kai Zhang, Luyao Wang, Huili Chen, Lili Deng, Mengling Hu, Ziqiang Wang, Yiluo Xie, Chaoqun Lian, Xiaojing Wang, Xiaojing Wang, Jing Zhang

“…Finally, the key gene SNRPA was screened, and a series of in vitro experiments were performed on LUAD cell lines to explore the role of SNRPA in LUAD.ResultLUAD patients could be accurately categorized into subtypes based on the nucleotide metabolism-related prognostic risk score (NMBRS). …”
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NLRX1 limits inflammatory neurodegeneration in the anterior visual pathway by Alexander J. Gill, Matthew D. Smith, Danny Galleguillos, Thomas Garton, Jackson W. Mace, Sachin P. Gadani, Swati Kumar, Aayush Pokharel, Krista Solem, Saahith Potluri, Omar Hussein, Giuliana Sardi Rogines, Arihant Singh, Annatje Clark, Peter A. Calabresi, Marjan Gharagozloo

“…To minimize the effects of Nlrx1 −/− on peripheral lymphocyte priming during EAE, we performed adoptive transfer experiments, in which activated myelin-specific T cells were transferred into lymphocyte-deficient Rag −/− or Nlrx1 −/− Rag −/− mice. …”
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Socio-economic inequalities in minimum dietary diversity among Ethiopian children aged 6–23 months: a decomposition analysis by Nigusu Worku, Amare Mesfin Workie, Lamrot Yohannes, Mihret Getnet, Mihret Getnet, Wubshet Debebe Negash, Wubshet Debebe Negash, Adina Yeshambel Belay, Lakew Asmare, Hiwot Tadesse Alemu, Demiss Mulatu Geberu, Melak Jejaw, Kaleb Assegid Demissie, Misganaw Guadie Tiruneh, Asebe Hagos

“…A probit model decomposition analysis was performed. The concentration index (CIX) and curve were used to examine household wealth status and maternal education level inequalities in the MDD.ResultsThe total weighted prevalence of MDD among children under the age of 5 in Ethiopia was 13.5%. …”
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Examining internet use for health information seeking and influencing factors among undergraduate health science students in Southwest Ethiopia by Alex Ayenew Chereka, Fikadu Wake Butta, Addisalem Workie Demsash, Sisay Yitayh Kassie, Adamu Ambachew Shibabaw, Abiy Tasew Dubale, Geleta Nenko Dube, Mekonnen Kenate Hunde, Wubalem Dilie Limeneh, Amare Dagnew Fentahun, Milkias Dugassa Emanu, Mekashaw Tareke Abebe, Gemeda Wakgari Kitil

“…Data entry and analysis were performed using EpiData V4.6 and STATA V17, respectively. …”
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The therapeutic potential of PX-478 in a murine model of pelvic organ prolapse by Wei-Min Fan, Yu-Qi Yang, Li-Wen Zhang, Xiao-Hui Mei, Ke Sun, Duan-Qing Wu, Ying Yang, Chun-Fang Duan, Jun Ye, Ru-Jun Chen

“…The analysis of fibroblasts, macrophage and inflammation was performed through Masson staining, immunofluorescence, and ELISA. …”
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Genome-Wide Scans for Selection Signatures in Haimen Goats Reveal Candidate Genes Associated with Growth Traits by Zhen Zhang, Jiafeng Lu, Yifei Wang, Zhipeng Liu, Dongxu Li, Kaiping Deng, Guomin Zhang, Bingru Zhao, Peihua You, Yixuan Fan, Feng Wang, Ziyu Wang

“…However, knowledge regarding the genetic structure and germplasm characteristics of Haimen goats remains limited. In this study, we performed 20× whole-genome resequencing of 90 goats (60 Haimen goats and 30 Boer goats) to identify single-nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) associated with growth traits. …”
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Lactiplantibacillus plantarum (CECT7484 and CECT7485) and Pedioccoccus acidilactici (CECT7483) enhance actin cytoskeleton and CYP1A1 expression restoring epithelial permeability al... by Maria Raffaella Barbaro, Francesca Bianco, Cesare Cremon, Giovanni Marasco, Francesca Bonomini, Marta Palombo, Cecilia Delprete, Marta Perez, Jordi Espadaler-Mazo, Vincenzo Stanghellini, Simone Guglielmetti, Giovanni Barbara

“…RNA was extracted from Caco-2 cells and used to perform qPCR analyses, to evaluate the expression of ZO-1 and β-actin, and RNAseq to evaluate the transcriptomic profile. …”
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Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis by Camila F. Oliveira-Toré, Amarilis G. Moraes, Gabriela F. Martinez, Janisleya S. F. Neves, Luciana C. Macedo, Marco A. Rocha-Loures, Marília G. Quirino, Hugo V. Alves, Ana M. Sell, Jeane E. L. Visentainer

“…The polymorphisms genotyped by PCR-RFLP were TLR2 rs5743708, TLR6 rs5743810, and TLR9 rs5743836 and rs187084. The HLA-B∗27 was performed by PCR-SSP. Results. Logistic regression analysis showed a strong association between SNPs in TLR2 and TLR9 and susceptibility to SpA (OR=12.56; CI=6.5-25.9 and OR=1.62; CI=1.20-2.21, respectively). …”
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Optimization of a micro-scale air–liquid-interface model of human proximal airway epithelium for moderate throughput drug screening for SARS-CoV-2 by Chandani Sen, Tammy M. Rickabaugh, Arjit Vijey Jeyachandran, Constance Yuen, Maisam Ghannam, Abdo Durra, Adam Aziz, Kristen Castillo, Gustavo Garcia, Arunima Purkayastha, Brandon Han, Felix W. Boulton, Eugene Chekler, Robert Garces, Karen C. Wolff, Laura Riva, Melanie G. Kirkpatrick, Amal Gebara-Lamb, Case W. McNamara, Ulrich A. K. Betz, Vaithilingaraja Arumugaswami, Robert Damoiseaux, Brigitte N. Gomperts

“…As a result, most of the drug screening assays for respiratory viruses are performed on commercial cell line-based 2D cultures that provide limited translational ability. …”
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Knowledge of prevention and treatment of infectious diarrhea among parents of children under five with diarrhea in Anhui province: an outpatient-clinic-based questionnaire survey by Yuanfang SUN, Yongkang XIAO, Lei GONG, Hongyuan WEI, Wanwan MA, Shuo FENG, Qiang ZHANG, Linlin LI, Qile WU, Jiabing WU

“…A multivariate analysis of factors influencing parental knowledge of diarrhea prevention was performed using binary logistic regression, adjusting for potential confounders. …”
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A comparative analysis in monitoring 24-hour urinary copper in wilson disease: sampling on or off treatment? by Isabelle Mohr, Patrick Lamade, Christophe Weber, Viola Leidner, Sebastian Köhrer, Alexander Olkus, Matthias Lang, Andrea Langel, Patrischia Dankert, Melanie Greibich, Silke Wolf, Holger Zimmer, Patrick Michl, Aurélia Poujois, Karl Heinz Weiss, Uta Merle

“…The study shows discordant results for both sampling methods in approximately half of the patients, revealing that interpretation of 24 h-UCE with respect to chelator-dosing decisions should be performed with caution.…”
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Adjustment of the Framingham index by abdominal aortic calcification scores enables a more accurate prediction of long-term cardiac events in general population aged 40 years and a... by Xiao-Jun Liu, Li Wang, Li-sha Zhang, Li-Fei Cao, Zhao-Fei Wan, Jia-Hong Xue, Yan-Ru He, Xin-Hong Wang, Xiao-Fang Bai

“…The Spearman’s rank correlation was performed to explore the correlation between the AAC score and FRS. the Kaplan-Meier method was used to make cumulative cardiac death curve. …”
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Mortality in patients with giant cell arteritis in Spain: results from the ARTESER registry by Juan Molina-Collada, Marta Domínguez-Álvaro, Rafael B. Melero-González, Eugenio de Miguel, Maite Silva-Díaz, Jesús Alejandro Valero Jaimes, Ismael González, Julio Sánchez Martín, Javier Narváez, Joan Calvet, Ivette Casafont-Solé, Jose A Román Ivorra, Selene Labrada Arrabal, Margarida Vasques Rocha, Carlota L Iñiguez, María Sagrario Bustabad Reyes, Cristina Campos Fernández, María Alcalde Villar, Antonio Juan Mas, Ricardo Blanco, on behalf of the ARTESER Project Collaborative Group

“…Kaplan-Meier method was performed for survival analysis. The factors influencing mortality were analyzed using Cox regression model. …”
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Influence of catheter ablation for atrial fibrillation on atrial and ventricular functional mitral regurgitation by Masaharu Masuda, Kimiko Sekiya, Mitsutoshi Asai, Osamu Iida, Shin Okamoto, Takayuki Ishihara, Kiyonori Nanto, Takashi Kanda, Takuya Tsujimura, Yasuhiro Matsuda, Yosuke Hata, Hiroyuki Uematsu, Taku Toyoshima, Naoko Higashino, Toshiaki Mano

“…Baseline and 6 month follow‐up echocardiography were performed to assess MR, LA, and LV sizes. MR improvement after ablation was comparable in the AFMR (64%) and VFMR groups (52%, P = 0.20). …”
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Cluster randomised controlled trial to examine medical mask use as source control for people with respiratory illness by Abrar Ahmad Chughtai, Chandini Raina MacIntyre, Yi Zhang, Quanyi Wang, Bayzidur Rahman, Haiyan Zhang, Holly Seale, Daitao Zhang, Yanhui Chu

“…Since 43 index cases in the control arm also used a mask during the study period, an as-treated post hoc analysis was performed by comparing outcomes among household members of index cases who used a mask (mask group) with household members of index cases who did not use a mask (no-mask group).Main outcome measure Primary outcomes measured in household members were clinical respiratory illness, ILI and laboratory-confirmed viral respiratory infection.Results In an intention-to-treat analysis, rates of clinical respiratory illness (relative risk (RR) 0.61, 95% CI 0.18 to 2.13), ILI (RR 0.32, 95% CI 0.03 to 3.13) and laboratory-confirmed viral infections (RR 0.97, 95% CI 0.06 to 15.54) were consistently lower in the mask arm compared with control, although not statistically significant. …”
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High Frequency of AIFM1 Variants and Phenotype Progression of Auditory Neuropathy in a Chinese Population by Hongyang Wang, Dan Bing, Jin Li, Linyi Xie, Fen Xiong, Lan Lan, Dayong Wang, Jing Guan, Qiuju Wang

“…A follow-up study was performed on 30 previously reported AIFM1-positive subjects, 16 follow-up cases (53.3%) were included in this study, and follow-up periods were recorded from 1 to 23 years with average 9.75±9.89 years. …”
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Effects of antibiotic therapy on the early development of gut microbiota and butyrate-producers in early infants by Jun Qiu, Jun Qiu, Sha Wu, Sha Wu, Ruiwen Huang, Zhenyu Liao, Xiongfeng Pan, Kunyan Zhao, Kunyan Zhao, Yunlong Peng, Yunlong Peng, Shiting Xiang, Yunhui Cao, Yunhui Cao, Ye Ma, Zhenghui Xiao, Zhenghui Xiao

“…Therefore, comprehending the effects of commonly used antibiotic therapy on the gut microbiota and butyrate-producers in early infants could provide information for therapeutic decision-making in the NICU.ObjectivesTo explore the effects of antibiotic therapy on the early development of gut microbiota and butyrate-producers in early infants.MethodsA total of 72 infants were included in the study. We performed 16S rRNA sequencing on stool swab samples collected from neonatal intensive care unit patients who received amoxicillin-clavulanic acid (AC, n = 10), moxalactam (ML, n = 28) and non-antibiotics (NA, n = 34). …”
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Spatiotemporal Assessment of PM2.5 Exposure of a High-risk Occupational Group in a Southeast Asian Megacity by Jarl Tynan Collado, Jose Gabriel Abalos, Imee de los Reyes, Melliza T. Cruz, Gabrielle Frances Leung, Katrina Abenojar, Carlos Rosauro Manalo, Bernell Go, Christine L. Chan, Charlotte Kendra Gotangco Gonzales, James Bernard B. Simpas, Emma E. Porio, John Q. Wong, Shih-Chun Candice Lung, Maria Obiminda L. Cambaliza

“…To the best of our knowledge, this study is the first in the country to perform a detailed characterization of the exposure of a high-risk occupational group to PM2.5. …”
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