Beneficial effects of fermented jujube pulp in relieving loperamide hydrochloride-induced constipation in male ICR mice by Jing Li, Xinyue Yu, Xinxing Xu, Peipei Tang, Shuang Bi, Xiaopu Ren, Yong Shi, Jihong Wu, Fei Lao

“…Defecation function, serum gastrointestinal regulatory peptide, fecal short-chain fatty acids (SCFAs) and gut microbial composition were assessed. …”
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Sex Differences in the Prognostic Value of Circulating Biomarkers in Patients Presenting With Acute Chest Pain by Gard Mikael Sæle Myrmel, MD, Nasir Saeed, Ole Thomas Steiro, MD, Hilde Lunde Tjora, MD, PhD, Jørund Langørgen, MD, PhD, Rune Oskar Bjørneklett, MD, PhD, Øyvind Skadberg, MD, Vernon Vijay Singha Bonarjee, MD, PhD, Øistein Rønneberg Mjelva, MD, Eva Ringdal Pedersen, MD, PhD, Kjell Vikenes, MD, PhD, Torbjørn Omland, MD, PhD, Kristin Moberg Aakre, MD, PhD

“…Methods: High-sensitivity cardiac troponin (hs-cTn), hs-cTnI, N-terminal pro-B-type natriuretic peptide (NT-proBNP), growth differentiation factor (GDF)-15, and C-reactive protein (CRP) concentrations were measured in 1,476 patients admitted with suspected NSTE-ACS. …”
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Type 2 diabetes mellitus’ impact on heart failure patients’ exercise tolerance: a focus on maximal fat oxidation during exercise by Huiying Zhu, Huiying Zhu, Jianchao Pan, Jianxuan Wen, Jianxuan Wen, Xiaojing Dang, Xiaojing Dang, Xiankun Chen, Xiankun Chen, Yunxiang Fan, Yunxiang Fan, Weihui Lu, Weihui Lu, Wei Jiang, Wei Jiang

“…After adjusting for age, sex, body mass index (BMI), log N-terminal pro-B type natriuretic peptide (log NT-proBNP), left ventricular ejection fraction (LVEF), hemoglobin, renal function, coronary heart disease and hypertension, the peak VO2 was lower in the T2DM group compared to the non-T2DM group with a mean difference (MD) of −2.0 ml/kg/min [95% confidence interval (CI), −3.18 to −0.82, P < 0.01]. …”
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5-deoxy-rutaecarpine protects against LPS-induced acute lung injury via inhibiting NLRP3 inflammasome-related inflammation by Jinque Luo, Jinque Luo, Xin Li, Xin Li, Li Zhang, Li Zhang, Meijing Deng, Jieyang Zhao, Jinghuan Zhang, Wenyu Tang, Qinghua Guo, Ling Wang

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Expression Analysis and SNP Mining of the Parkin Co-Regulated Gene (PACRG) and parkin in Penaeus vannamei Against White Spot Syndrome Virus by Qian XUE, Xupeng LI, Yang LI, Sheng LUAN, Kun LUO, Jie KONG, Qun XING, Xianhong MENG

“…Parkin is predicted to contain UBQ and IBR structural domains and a signal peptide structure. Amino acid sequence alignment and phylogenetic tree analysis showed that the homology of PACRG between P. vannamei and Penaeus japonicus was the highest at 89.70% similarity. …”
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GrB-Fc-KS49, an anti-EMP2 granzyme B fusion protein therapeutic alters immune cell infiltration and suppresses breast cancer growth by Ana Alvarez de Cienfuegos, Lawrence H Cheung, Khalid A Mohamedali, Michael G Rosenblum, Brian Aguirre, Cheng-Hsiang Lu, Anubhav Chandla, Nidhi Kejriwal, Lucia Liu, Ann M Chan, SuYin Kok, Sergio Duarte, David Casero, Madhuri Wadehra

“…The construct’s pharmacokinetics (PK), toxicity profile, and in vivo efficacy were also evaluated.Results GrB-Fc-KS49 exhibited comparable GrB enzymatic activity to commercial GrB, as well as high affinity to an EMP2 peptide, with the dissociation constant in the picomolar range. …”
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Large-scale manufacturing and characterization of CMV-CD19CAR T cells by Stephen J Forman, Xiuli Wang, Ryan Urak, Miriam Walter, Min Guan, Tianxu Han, Vibhuti Vyas, Sheng-Hsuan Chien, Brenna Gittins, Mary C Clark, Sally Mokhtari, Angelo Cardoso, Don J Diamond, John Zaia, Ryotaro Nakamura

“…Here we developed a clinical platform for generating CMV-CD19CAR T cells.Methods Peripheral blood mononuclear cells (PBMCs) collected from CMV-seropositive healthy donors were stimulated with a good manufacturing practices-grade PepTivator overlapping CMVpp65 peptide pool and enriched for CMV-responsive interferon γ (IFNγ)+T cells using IFNγ Catchmatrix, within the CliniMACS Prodigy Cytokine Capture System (Miltenyi Biotec). …”
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Integrated microfluidics-based construction of anti-BTN2A2 gel droplet cell preparations for non-invasive tumor-infiltrating lymphocyte therapy by Yishen Tian, Jingxuan Li, Na Yang, Yang Zhao, Jiancao Zuo, Hang Xiong, Yiwen Pan, Li Xiao, Min Su, Feng Han, Zhixu He, Rong Hu

“…This preparation was surface modified with BTN2A2 antibodies and internally contained T cells isolated from the blood of tumor hosts, along with simulated collagen peptide CMP. Specifically, the cell preparation exerted its anti-tumor effects through multiple mechanisms: Firstly, the surface BTN2A2 antibodies effectively inhibited the proliferation of Tregs and MDSCs within the tumor microenvironment; Secondly, leveraging the T cell antigen receptors (TCRs) present in the blood T cells, which were similar to those of tumor-infiltrating lymphocytes, significantly enhanced their targeting and cytotoxic capabilities; Furthermore, the CMP component within the droplets effectively promoted the infiltration of T cells into tumor tissues. …”
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Therapeutic effects of composite probiotics derived from fermented camel milk on metabolic dysregulation and intestinal barrier integrity in type 2 diabetes rats by Tabusi Manaer, Tabusi Manaer, Tabusi Manaer, Tabusi Manaer, Jialehasibieke Sailike, Xin Sun, Baheban Yeerjiang, Baheban Yeerjiang, Baheban Yeerjiang, Baheban Yeerjiang, Xinhua Nabi, Xinhua Nabi, Xinhua Nabi, Xinhua Nabi

“…Measurements over 6 weeks included body weight (BW), fasting blood glucose (FBG), oral glucose tolerance test (OGTT), glycated hemoglobin (HbA1c), C-peptide (CP), lipid profiles, inflammatory markers, fecal short-chain fatty acids (SCFAs), and tight junction protein expression in colonic tissues.ResultsHigh-dose CPCM significantly increased BW by 22.2% (p < 0.05) and reduced FBG by 6.5 mmol/L (p < 0.001). …”
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Clinical Characteristics of Fulminant Type 1 Diabetes Compared with Typical Type 1 Diabetes: One-Year Follow-Up Study from the Guangdong T1DM Translational Medicine Study by Daizhi Yang, Yongwen Zhou, Sihui Luo, Xueying Zheng, Ping Ling, Liling Qiu, Wen Xu, Hua Liang, Bin Yao, Jianping Weng, Jinhua Yan

“…Both fasting and postprandial C-peptide levels (FCP and PCP, respectively) in FT1DM were significantly lower (P<0.001). …”
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The HFA‐PEFF score identifies ‘early‐HFpEF’ phenogroups associated with distinct biomarker profiles by Michiel T.H.M. Henkens, Anne‐Mar vanOmmen, Sharon Remmelzwaal, Gideon B. Valstar, Ping Wang, Job A.J. Verdonschot, Mark R. Hazebroek, Leonard Hofstra, Vanessa P.M. vanEmpel, Joline W.J. Beulens, Hester M. denRuijter, Stephane R.B. Heymans

“…The top eight biomarkers—N‐terminal prohormone brain natriuretic peptide, growth differentiation factor‐15, matrix metalloproteinase‐2, osteoprotegerin, tissue inhibitor of metalloproteinase‐4, chitinase‐3‐like protein 1, insulin‐like growth factor‐binding protein 2, and insulin‐like growth factor‐binding protein 7—are mainly involved in inflammation and extracellular matrix remodelling, which are currently proposed key processes in HFpEF pathophysiology. …”
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From the depths of the Java Trench: genomic analysis of Priestia flexa JT4 reveals bioprospecting and lycopene production potential by Ocky K. Radjasa, Ray Steven, Yosua Natanael, Husna Nugrahapraja, Septhy K. Radjasa, Tati Kristianti, Maelita R. Moeis, Joko P. Trinugroho, Haekal B. Suharya, Alfito O. Rachmatsyah, Ari Dwijayanti, Mutiara R. Putri, Charlie E. de Fretes, Zen L. Siallagan, Muhammad Fadli, Rafidha D. A. Opier, Jandinta D. Farahyah, Viana Rahmawati, Meirifa Rizanti, Zalfa Humaira, Ary S. Prihatmanto, Nugroho D. Hananto, R. Dwi Susanto, Agus Chahyadi, Elfahmi, Neil Priharto, Kamarisima, Fenny M. Dwivany

“…Moreover, our analysis uncovered eight biosynthetic gene clusters (BGCs) using the common genome mining pipeline, antiSMASH. Two non-ribosomal peptide synthetase (NRPS) BGCs within these clusters exhibited the potential to generate novel biological compounds. …”
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Clinical implication of NT-proBNP to predict mortality in patients with acute type A aortic dissection: a retrospective cohort study by Rui Zhang, Hao Wang, Chenxi Song, Rui Fu, Kefei Dou, Shuai Liu, Qianqian Liu, Xiaohui Bian, Weida Liu, Chengang Zhu, Jingjing Gao, Sheng Yuan, Xinming Cui, Sijia Qin, Yumeng Li

“…We aimed to investigate the prognostic value of N-terminal pro-B type natriuretic peptide (NT-proBNP) among this critically ill population.Design The design of this study was a retrospective cohort study.Setting The study population was recruited in the Emergency Department of Fuwai hospital in China from 2018 to 2020.Participants We consecutively enrolled 829 patients with acute type A aortic dissection and measurable baseline NT-proBNP.Primary outcome The primary endpoint was 1-year all-cause death.Results Based on tertiles of NT-proBNP (pg/mL), patients were stratified into low (≤150.3, n=276), intermediate (150.3–667.6, n=277) and high (&gt;667.6, n=276) NT-proBNP groups. …”
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Effects of <i>Enterococcus faecalis</i> Supplementation on Growth Performance, Hepatic Lipid Metabolism, and mRNA Expression of Lipid Metabolism Genes and Intestinal Flora in Geese by Siyu Sun, Yujie Zhao, Zhen Pang, Baoxia Wan, Jiaqi Wang, Zhenyu Wu, Qiuju Wang

“…Serum insulin levels were significantly elevated on day 25, and glucagon-like peptide-1 (GLP-1) levels were significantly increased on day 45 (<i>p</i> < 0.05). …”
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TCR-engaging scaffolds selectively expand antigen-specific T-cells with a favorable phenotype for adoptive cell therapy by Marco Donia, Özcan Met, Julie Westerlin Kjeldsen, Sine Reker Hadrup, Inge Marie Svane, Arianna Draghi, Anders Handrup Kverneland, Christina Heeke, Siri Amanda Tvingsholm, Marcus Svensson Frej, Vibeke Mindahl Rafa, Ulla Kring Hansen, Maria Ormhøj, Alexander Tyron, Agnete W P Jensen, Mohammad Kadivar, Amalie Kai Bentzen, Kamilla K Munk, Gitte N Aasbjerg, Jeppe S H Ternander, Tripti Tamhane, Christian Schmess, Samuel A. Funt, Søren Nyboe Jakobsen

“…Here, in this study, we present a novel technology for ex vivo expansion of antigen-specific T-cells; artificial antigen-presenting scaffolds (Ag-scaffolds) consisting of a dextran-polysaccharide backbone, decorated with combinations of peptide-Major Histocompatibility Complex (pMHC), cytokines and co-stimulatory molecules, enabling coordinated stimulation of antigen-specific T-cells.Methods The capacity of Ag-scaffolds to expand antigen-specific T-cells was explored in ex vivo cultures with peripheral blood mononuclear cells from healthy donors and patients with metastatic melanoma. …”
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A Cardiac‐Targeting and Anchoring Bimetallic Cluster Nanozyme Alleviates Chemotherapy‐Induced Cardiac Ferroptosis and PANoptosis by Junyue Xing, Xiaohan Ma, Yanan Yu, Yangfan Xiao, Lu Chen, Weining Yuan, Yingying Wang, Keyu Liu, Zhiping Guo, Hao Tang, Kelong Fan, Wei Jiang

“…To enhance cardiac targeting, the atrial natriuretic peptide is conjugated to the AuRu nanozyme. These cardiac‐targeted bimetallic cluster nanozymes, with their anchoring capability, effectively reduce DOX‐induced cardiomyocyte ferroptosis and PANoptosis without compromising tumor treatment efficacy. …”
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Exploring the role of ESR1 mutations in metastatic hormone receptor-positive breast cancer T cell immune surveillance disruption by Morgane Lopez, Laurie Spehner, Fabrice André, Julien Viot, Evan Seffar, Amélie Marguier, Elsa Curtit, Guillaume Meynard, Erion Dobi, Sylvain Ladoire, Romain Boidot, Romain Loyon, Valentin Derangere, François-Clément Bidard, Christophe Borg, Laura Mansi, Marie Kroemer

“…Methods We identified candidate ESR1-derived peptides by predictive software (SYFPEITHI and NetMHCpan 3.0). …”
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Effectiveness and tolerability of rimegepant in the acute treatment of migraine: a real-world, prospective, multicentric study (GAINER study) by Luigi Francesco Iannone, Gloria Vaghi, Gabriele Sebastianelli, Francesco Casillo, Antonio Russo, Marcello Silvestro, Francesca Pistoia, Giorgio Dalla Volta, Matteo Cortinovis, Alberto Chiarugi, Danilo Antonio Montisano, Maria Pia Prudenzano, Sabina Cevoli, Edoardo Mampreso, Gianluca Avino, Marina Romozzi, Mariarosaria Valente, Carla Fasano, Stefania Battistini, Antonio Granato, Elisa Maria Piella, Innocenzo Rainero, Raffaele Ornello, Roberto De Icco, On behalf of the Italian Headache Registry (RICe) Study Group

“…Abstract Background Rimegepant, a novel oral calcitonin gene-related peptide receptor antagonist, has been recently approved for the acute migraine treatment. …”
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The diagnostic value of the combined application of blood lipid metabolism markers and interleukin-6 in osteoporosis and osteopenia by Liping Fan, Jiahao Chen, Chong Chen, Yongwei Zhang, Yeqing Yang, Zhe Chen

“…Additionally, apolipoprotein A1 (ApoA1) was negatively correlated with BMD only in females, and the ApoA1/ApoB ratio was positively correlated with BMD only in males. (2) FFAs and IL-6 were positively correlated with β-CrossLaps peptide in males. However, for females, TC, ApoB, LDL-C, and IL-6 were negatively correlated with 25-hydroxyvitamin D. …”
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Effect of Yanshiqiangxin Decoction on Cardiomyocyte Apoptosis in Heart Failure Rats Based on PI3K/Akt/Caspase-9 Signaling Pathway by ZHU Sixing, YAN Shiyun, XU Yan, QIN Yan, CHEN Liyun, JIA Meijun

“…One day after the last gavage, M-mode echocardiography [left ventricular ejection fraction (LVEF), left ventricular short-axis shortening (LVFS), left ventricular internal dimension diastole (LVIDd) and left ventricular internal dimension systole (LVIDs)] was performed through an ultra-high resolution small animal ultrasound imaging system; B-type natriuretic peptide (BNP) was measured by ELISA method; the apoptosis of cardiomyocytes was observed through laser confocal microscopy and the apoptosis index was calculated; the expression of apoptosis-related proteins Bax, Bcl-2 and PI3K/Akt/Caspase-9 signalling pathway proteins were detected by Western blot method.Results① Cardiac ultrasound test results: compared with the normal group, the left ventricular anterior wall motion waveform of the model group was not obvious, close to linear motion, and LVEF and LVFS decreased significantly (<italic>P</italic>&lt;0.05), LVIDd and LVIDs increased significantly (<italic>P</italic>&lt;0.05), the left ventricle was significantly enlarged and the contractility was reduced. …”
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