CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp by Jie Zhang, Liqiang Zhang, Xiaoman Wu, Minhui Tao, Yang Chen, Mingxian Chang

“…Compared with WT grass carp without infection, PI4KB F0 juvenile grass carp crispants without infection or WT grass carp infected with GCRV-II, higher expression levels for many genes involved in metabolic diseases and viral infections were observed in the liver from PI4KB F0 juvenile grass carp crispants infected with GCRV-II. …”
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Permafrost pore structure and its influence on microbial diversity: Insights from X-ray computed tomography by Nathan D. Blais, Joy M. O’Brien, Hannah Holland-Moritz, Lauren Farnsworth, Robyn A. Barbato, Thomas A. Douglas, Alexandra Contosta, Julie Bobyock, Erin C. Rooney, Taylor Sullivan, Jessica Gilman Ernakovich

“…Additionally, we identified pore connectivity as a potential driver of microbial diversity and provided evidence that conditions before the formation of permafrost exert a strong legacy effect on currently observed permafrost microbial diversity. These insights help to explain how soil physical structure acts to influence microbial communities in this extreme environment.…”
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miR‐224‐5p Suppresses Non‐Small Cell Lung Cancer via IL6ST‐Mediated Regulation of the JAK2/STAT3 Pathway by Jiao Tian, Yiming He, Zihui Zhang, Yuxin Zhu, Haixia Ren, Liang Zhang, Lei Li, Wei Li, Weidong Zhang, Ting Xiao, Honggang Zhou, Xiaoping Li

“…We identified IL6ST (interleukin 6 signal transducer) as a binding target of miR‐224‐5p. We observed that miR‐224‐5p could bind to and inhibit IL6ST expression and JAK2/STAT3 signaling pathway, and the inhibition of NSCLC tumor growth and JAK2/STAT3 pathway by miR‐224‐5p could be reversed by IL6ST overexpression. …”
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Whole-genome sequencing identifies novel loci for keratoconus and facilitates risk stratification in a Han Chinese population by Yinghao Yao, Xingyong Li, Lan Wu, Jia Zhang, Yuanyuan Gui, Xiangyi Yu, Yang Zhou, Xuefei Li, Xinyu Liu, Shilai Xing, Gang An, Zhenlin Du, Hui Liu, Shasha Li, Xiaoguang Yu, Myopia Associated Genetics Intervention and Consortiums, Hua Chen, Jianzhong Su, Shihao Chen

“…Additionally, a significant correlation of allelic effects was observed for suggestive SNPs between the largest multi-ethnic meta-genome-wide association study (GWAS) and our study. …”
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An Overview of Patient-reported Outcomes for Men with Prostate Cancer: Results from the PIONEER Consortium by Sebastiaan Remmers, Katharina Beyer, Tariq A. Lalmahomed, Peter Prinsen, Nicole J.E. Horevoorts, Nora Tabea Sibert, Christoph Kowalski, Francesco Barletta, Oliver Brunckhorst, Giorgio Gandaglia, Jochem R.N. van der Voort van Zyp, Emma J. Smith, Andre Deschamps, Laurence Collette, Philip Cornford, Susan Evans-Axelsson, James N’Dow, Mieke Van Hemelrijck, Monique J. Roobol, Lionne D.F. Venderbos

“…Most men were diagnosed with Gleason ≤7 disease, and T1 or T2 PCa. We observed that sexual dysfunction was the most affected PRO and found large differences between data sources. …”
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An open-label, randomized, post-authorization study of mirabegron in Chinese participants with overactive bladder by Zhipeng Zhang, Deyi Luo, Zhong Chen, Peng Zhang, Ganping Zhong, Keji Xie, Zhuoqun Xu, Xudong Li, Jianye Wang, Yingfan Yang, Farid Abdul Hadi, Arianne Schild

“…Results: Statistically significant reduction (p<0.001) from baseline to Week 12 was observed in mean micturitions per 24 h for participants randomized to mirabegron 50 mg/day: mean±standard error: 11.71±0.43 at baseline, 7.80±0.24 at Week 12; adjusted mean change: −3.73 (95% confidence interval −4.30 to −3.16). …”
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Compatibility and antimicrobial activity of silver nanoparticles synthesized using Lycopersicon esculentum peels by Esraa Ali, Samah H. Abu-Hussien, Esraa Hesham, Shimaa Ahmed, Habiba Mostafa, Ahmed Gamal, Salwa M. El-Sayed, Bahaa Hemdan, Ashraf Bakry, Naglaa M. Ebeed, Hesham Elhariry, Ahmed Galal, Basma T. Abd-Elhalim

“…The cytotoxicity effect was observed at a half-maximal inhibitory concentration (IC50) of 200.53 μg/ml on human colon NCM460D normal cells.…”
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Reproductive Efficiency of Nelore Cows in Fixed-Time Artificial Insemination Programs with Early Resynchronization by Larissa de Paiva Nunes Gonçalves, Alisson Jordão Prado, Aline Pacheco, Yana Eliza Feitosa de Almeida, Pietro Sampaio Baruselli, Welligton Conceição da Silva, Antônio Humberto Hamad Minervino, Jucelane Salvino de Lima, Kedson Alessandri Lobo Neves

“…The cumulative pregnancy rate after the 1st FTAI, 1st, and 2nd resynchronizations was 80.77% in the R30 group and 82.91% in the R22 group (<i>p</i> = 0.643), with no significant difference observed. Follicular dynamics were also monitored, with ovulation occurring in 78.95% of cases in the 1st FTAI, 91.67% in the 1st resynchronization, and 88.98% in the 2nd resynchronization. …”
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Achieving high strength-ductility synergy in Mg-6Sn-3Zn-0.3Zr (wt.%) alloy via a combination of casting, pre-treatment and hot extrusion by Tianshui Zhou, Yaqin Li, Feifei Guo, Qinglin Li, Zhi Jia, Dexue Liu

“…Strong random rather than basal texture is observed both in as-cast and pre-treatment samples. …”
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Efficacy and safety of the pulsed electromagnetic field in osteoarthritis: a meta-analysis by Ziying Wu, Xiang Ding, Guanghua Lei, Chao Zeng, Jie Wei, Jiatian Li, Hui Li, Tuo Yang, Yang Cui, Yilin Xiong, Yilun Wang, Dongxing Xie

“…Similarly, comparing with the sham–control treatment, significant function improvement was observed in the PEMF group in both knee and hand OA patients (SMD=−0.34, 95% CI −0.53 to –0.14, p=0.0006, and SMD=−1.49, 95% CI −2.12 to –0.86, p&lt;0.00001, respectively), but not in patients with cervical OA. …”
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Incidence of shoulder dislocations in the UK, 1995–2015: a population-based cohort study by Gary S Collins, Sarah E Lamb, Sally Hopewell, Daniel Prieto-Alhambra, Andrew Judge, Amar Rangan, Andrew J Carr, Jonathan L Rees, Nigel K Arden, Katherine Edwards, Tim A Holt, Anjali Shah, Antonella Delmestri, Rafael A Pinedo-Villanueva

“…The highest incidence was observed in men aged 16–20 years (80.5 per 100 000 person-years; 95% CI 80.5 to 80.6). …”
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Position in proton Bragg curve influences DNA damage complexity and survival in head and neck cancer cells by Tim Heemskerk, Celebrity Groenendijk, Marta Rovituso, Ernst van der Wal, Wouter van Burik, Konstantinos Chatzipapas, Danny Lathouwers, Roland Kanaar, Jeremy M.C. Brown, Jeroen Essers

“…These experimental observations are supported by the in silico study which demonstrates that irradiation at D20 leads to a 1.7-fold increase in complex DSBs with respect to the total number of strand breaks compared to P36 and P80. …”
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Short-term association between ambient air pollution and childhood non-allergic rhinitis: A time-series study at a National Children's Regional Medical Center, in Hefei, China, fro... by Wei Cui, Hongxia Gao, Liran Zhu, Yuanyuan Xu, Haipeng Liu

“…A total of 38233 NAR outpatients were included and a positive association and cumulative effects between air pollution and childhood NAR were observed. The relative risks (RRs) and 95 % confidence intervals (95 % CIs) for childhood NAR at the 75th percentile of NO2, SO2, PM10, PM2.5, O3, and CO against their minimum concentrations were 1.157 (95 % CI: 1.089, 1.23), 1.078 (1.005, 1.157), 1.168 (1.089, 1.252), 1.076 (1.02, 1.136), 1.033 (0.961, 1.11) and 1.07 (1.01, 1.134), respectively. …”
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Loneliness and Social Isolation with Risk of Incident Non-alcoholic Fatty Liver Disease, UK Biobank 2006 to 2022 by Ya Miao, Xiaoke Kong, Bin Zhao, Fang Fang, Jin Chai, Jiaqi Huang

“…The aims of this study were to determine whether loneliness and social isolation are independently associated with the risk of NAFLD and to explore potential mediators for the observed associations. Methods: In this large prospective cohort analysis with 405,073 participants of the UK Biobank, the status of loneliness and social isolation was assessed through self-administrated questionnaires at study recruitment. …”
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Gut microbiota interacting with vitamin D but not anandamide might contribute to the pathogenesis of preeclampsia: a preliminary study by Xiao-Qiang Han, Xiao-Qiang Han, Hui-Hui Jiang, Meng-Ling Chen, Meng-Ling Chen, De-Yang Han, De-Yang Han, Su-Fen Zhou, Su-Fen Zhou, Jin-Wen Wang, Shu-Shen Ji, Ling-Yun Wang, Jing-Wei Lou, Ming-Qun Li, Ming-Qun Li

“…Although no significant correlations were identified between the plasma AEA levels and serum VitD levels or any of the top 6 identified marker genera, a significant negative correlation was observed between VitD and Dorea, indicating VitD and gut microbiota have the potential to be combined targets for early diagnosis and management of PE.…”
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The AMD-associated genetic polymorphism CFH Y402H confers vulnerability to Hydroquinone-induced stress in iPSC-RPE cells by Angela Armento, Inga Sonntag, Ana-Cristina Almansa-Garcia, Merve Sen, Sylvia Bolz, Blanca Arango-Gonzalez, Ellen Kilger, Ruchi Sharma, Kapil Bharti, Rosario Fernandez-Godino, Berta de la Cerda, Simon J. Clark, Simon J. Clark, Simon J. Clark, Marius Ueffing

“…The exposure of RPE cells to RPE conditioned medium, normal human serum (NHS) and inactivated NHS (iNHS) had minimal impact on cell cytotoxicity and caspase activation, nor did the presence of purified soluble FH rescue the observed effects. Considering the known connection of oxidative stress to proteotoxic stress and degrading processes, we investigated the unfolded protein response (UPR) and autophagy. …”
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Correlations among lymphocyte count, white matter hyperintensity and brain atrophy in patients with ischemic stroke by Chenchen Liu, Dai Shi, Xiaoqiong Ni, Shoujiang You, Shoujiang You, Xiaofen Wu, Sheng Zhuang, Sheng Zhuang, Wu Cai, Liang Xu, Liang Xu

“…Furthermore, a notable inverse association was observed between continuous lymphocyte counts and WMH, PVH, DWMH, and brain atrophy. …”
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Higher 3-year recurrence-free survival rate in patients with complete pathological remission following neoadjuvant chemotherapy plus immunotherapy for esophageal cancer: a two-cent... by Hai Zhang, Hai Zhang, Hai Zhang, Haiquan He, Qingyi Feng, Bomeng Wu, Ying Chen, Zhenyang Zhang, Linrong Zhou, Cui Li, Wanli Lin, Jiangbo Lin, Jiangbo Lin

“…No significant differences were observed in the incidences of hemoglobin decrease, platelet decrease, liver function damage, elevated serum creatinine, diarrhea, radioactive pneumonia or immunotherapy-related pneumonia, and esophageal perforation. …”
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Comparative Analysis of Automated and Handheld Breast Ultrasound Findings for Small (≤1 cm) Breast Cancers Based on BI-RADS Category by Han Song Mun, Eun Young Ko, Boo-Kyung Han, Eun Sook Ko, Ji Soo Choi, Haejung Kim, Myoung Kyoung Kim, Jieun Kim

“…No significant differences were observed in other US findings or tumor sizes between the two modalities (all <i>p</i> > 0.05). …”
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Application of a new highly multiplexed amplicon sequencing tool to evaluate Plasmodium falciparum antimalarial resistance and relatedness in individual and pooled samples from Dsc... by Jacob M. Sadler, Alfred Simkin, Valery P. K. Tchuenkam, Isabela Gerdes Gyuricza, Isabela Gerdes Gyuricza, Abebe A. Fola, Kevin Wamae, Ashenafi Assefa, Ashenafi Assefa, Karamoko Niaré, Kyaw Thwai, Samuel J. White, William J. Moss, Rhoel R. Dinglasan, Sandrine Eveline Nsango, Christopher B. Tume, Jonathan B. Parr, Jonathan B. Parr, Jonathan B. Parr, Innocent Mbulli Ali, Jeffrey A. Bailey, Jeffrey A. Bailey, Jonathan J. Juliano, Jonathan J. Juliano, Jonathan J. Juliano, Jonathan J. Juliano

“…No K13 candidate or validated artemisinin partial resistance mutations were detected, but one low-level non-synonymous change was observed. Pf-SMARRT’s hypervariable targets, used to assess complexity of infections and parasite diversity and relatedness, showed similar levels and patterns compared to molecular inversion probe (MIP) sequencing. …”
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