Integrative physiological and transcriptome analysis unravels the mechanism of low nitrogen use efficiency in burley tobacco by Yuqing Feng, Yuanyuan Zhao, Yanjun Ma, Xiaolong Chen, Hongzhi Shi

“…Abstract Burley tobacco, a chlorophyll‐deficient mutant with impaired nitrogen use efficiency (NUE), generally requires three to five times more nitrogen fertilization than flue‐cured tobacco to achieve a comparable yield, which generates serious environmental pollution and negatively affects human health. …”
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Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice by Xiaoqiong Guo, Chunli Wang, Qian Zhu, Wenhua Dongchen, Xiaoling Zhang, Wei Li, Hui Zhang, Cui Zhang, Zar Ni Naing Nant Nyein, Mengting Li, Lijuan Chen, Dongsun Lee

“…In our investigation, we identified a rice mutant with defective chloroplasts in rice (Oryza sativa L.), named albino lethal 13 (osal13), which displayed a distinct albino phenotype in leaves, ultimately resulting in seedling lethality. …”
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First detection of the S989P+V1016G+D1763Y haplotype and expansion of voltage-gated sodium channel mutations in Aedes aegypti in Taiwan in 2016-2023. by Han-Hsuan Chung, Hwa-Jen Teng, Chin-Gi Huang, I-Jung Tsai, Hung-Jui Chen, Osamu Komagata, Shinji Kasai, Kun-Hsien Tsai, Shiu-Ling Chen

“…A T1520I mutation was detected for the first time and has since rapidly spread throughout Taiwan. The triple-mutant haplotype PGTFY was first documented in Ae. aegypti. …”
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Transcript Profiles of Stria Vascularis in Models of Waardenburg Syndrome by Linjun Chen, Lin Wang, Lei Chen, Fangyuan Wang, Fei Ji, Wei Sun, Hui Zhao, Weiju Han, Shiming Yang

“…The transcript profile of stria vascularis of Waardenburg syndrome was studied using Mitf-M mutant pig and mice models. Therefore, GO analysis was performed to identify the differential gene expression caused by Mitf-M mutation. …”
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Case report: A novel 11-bp deletion in exon 11 causing a frameshift in the C-terminal of the ALAS2 gene leading to X-linked sideroblastic anemia—a family study by Salam Al kindi, Salam Al kindi, Altaf Al-Mamari, Shoaib Al-Zadjali, Mohamed Al-Rawahi, Ali Al Madhani, Anil V. Pathare

“…The screening of the entire family’s kindred revealed that two other male siblings were also hemizygous for the same mutation with hypochromic microcytic anemia and tissue iron overload, whereas, three female siblings and their mother were heterozygous for the mutant allele. They did not have anemia or iron overload.…”
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Genomic and mutational analysis of Pseudomonas syringae pv. tagetis EB037 pathogenicity on sunflower by Jude E. Maul, John Lydon, Dilip Lakshman, Colin Willard, Hyesuk Kong, Daniel P. Roberts

“…Whole genome sequencing of Pstag strain EB037 and transposon-mutant derivatives, no longer capable of causing apical chlorosis, was conducted to improve understanding of the molecular basis of disease caused by this pathogen. …”
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Oncogenic RIT1 mutations confer ferroptosis vulnerability in lung adenocarcinoma by Ruilan Ma, Dian Yang, Peng Wang, Ziyi Zhang, Xuehong Zhang, Jialiang Song, Han Liu, Shuyan Liu, Yingqiu Zhang, Lijuan Zou

“…Importantly, RIT1 mutants markedly sensitized cells to ferroptosis induction, and RIT1 knockdown suppressed ferroptotic cell death. …”
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Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping by V. A. Devyatkin, A. A. Shklyar, A. Zh. Fursova, Yu. V. Rumyantseva, O. S. Kozhevnikova

“…The method consists of real-time PCR with a pair of primers and two oligonucleotide probes that are complementary to a sequence near a given locus in such a way that one probe is complementary to the wild-type allele, and the other to a mutant one. Advantages of this approach are its specificity, sensitivity, low cost, and quick results. …”
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The Arabidopsis RING‐Type E3 Ligase TEAR4 Controls Seed Germination by Targeting RGA for Degradation by Hao Yu, Yutao Wang, Yi Wang, Xing Wang Deng, Genji Qin

“…The tear1/2/3/4/5/6 sextuple mutant displayed delayed seed germination under the white or PhyB‐dependent light condition, and nearly no seed germination under the PhyA‐dependent light condition. …”
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Gene Therapy with HSV1-sr39TK/GCV Exhibits a Stronger Therapeutic Efficacy Than HSV1-TK/GCV in Rat C6 Glioma Cells by Lei-qing Li, Fang Shen, Xiao-yan Xu, Hong Zhang, Xiao-feng Yang, Wei-guo Liu

“…Therefore, In order to reduce the GCV dose required, we aim to compare the therapeutic efficacy of HSV1-sr39TK, an HSV1-TK mutant with increased GCV prodrug catalytic activity, with wildtype TK in C6 glioma cells. …”
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Engineering of Dihydroxy Biphenyl Dioxygenase Enzyme to Improve Its Performance In Removing PCBs: A Molecular Docking Study by Susan Yazdankhah, Alireza Zakeri, Saeed Khalili, Abolfazl Jahangiri, ZahraalSadat Hashemi, Alireza Heidari

“…Conclusion: The more negative binding energy indicates the greater stability of this interaction in the mutant enzyme. As a result, these mutations will be able to improve the strength of the enzyme function. …”
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Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence by Leilei Wu, Xueyan Zhu, Hang Long, Xinyu Huang, Shuying Zhu, Qi Xue, Ziao Li, Huasong Zou

“…The deletion of tolB resulted in similar phenotypic alterations as the OMPXan mutant in salt stress tolerance, cell membrane integrity, and the expression of hrpG, hrpX, hrpD6, and hrcC for pathogenicity. …”
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Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time by Jing Cui, Yajing Li, Hao Liu, Xu Jiang, Lili Zhang, Hongbo Dai, Xue Wang, Fei He, Mingna Li, Junmei Kang

“…In contrast, the flowering time of the cbfs mutant was earlier, and overexpression of MsCBF9 also increased the number and size of Arabidopsis rosette leaves. …”
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A Novel Pathogenic Splicing Mutation of OFD1 is Responsible for a Boy with Joubert Syndrome Exhibiting Orofaciodigital Spectrum Anomalies, Polydactyly and Retinitis Pigmentosa by Chen L, Zhao MF, Deng HW, Liao M, Fan LL, Zhong QB, Wang J, Li K, Wu ZH, Yin JY

“…This study presents a new case of JS and expands the mutant spectrum of OFD1, but also enhances our understanding of the mechanism by which OFD1 is associated with ciliosis.Keywords: Joubert syndrome, OFD1, splicing mutation, ciliosis…”
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The diagnostic accuracy of circulating free DNA for the detection of KRAS mutation status in colorectal cancer: A meta‐analysis by Wenli Xie, Li Xie, Xianrang Song

“…We extracted true‐positive (TP), false‐positive (FP), false‐negative (FN), true‐negative (TN) values, survival rate of CRC patients with mutant and wild‐type KRAS and calculated pooled sensitivity and specificity, positive/negative likelihood ratios [PLRs/NLRs], diagnostic odds ratios [DORs], and corresponding 95% confidence intervals [95% CIs]. …”
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Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida by Eveline-Marie Lammens, Daniel C. Volke, Alison Kerremans, Yannick Aerts, Maarten Boon, Pablo I. Nikel, Rob Lavigne

“…By finetuning expression levels of phi15rnap and introducing a phi15 lysozyme mutant that inhibits phi15 RNAP in uninduced conditions, a stringent system was created with 200-fold inducibility. …”
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The endonuclease activity of MCPIP1 controls the neoplastic transformation of epithelial cells via the c-Met/CD44 axis by Paulina Marona, Rafał Myrczek, Iga Piasecka, Judyta Gorka, Oliwia Kwapisz, Ewelina Pospiech, Janusz Rys, Jolanta Jura, Katarzyna Miekus

“…Proteomic analysis of mouse plasma revealed increased secretion of cancer-related proteins (CXCL13, CXCL16, and MMP2) in the MCPIP1-mutant group. Additionally, we revealed that MCPIP1 RNase activity regulates the expression of the stemness markers CD44 and CD133 and the phosphorylation of the c-Met receptor in tumor tissue samples. …”
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Decreased SynMuv B gene activity in response to viral infection leads to activation of the antiviral RNAi pathway in C. elegans. by Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H X Liu, Jennifer D Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J Tate, Taiowa A Montgomery, Gary Ruvkun

“…Caenorhabditis elegans mutants that disable RNAi are more sensitive to viral infection. …”
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Deletion of pagL and arnT genes involved in LPS structure and charge modulation in the Salmonella genome confer reduced endotoxicity and retained efficient protection against wild-... by Ram Prasad Aganja, Jun Kwon, Amal Senevirathne, John Hwa Lee

“…We evaluated two novel mutant SG strains, JOL3015 and JOL3016, carrying in-frame deletions in ΔlonΔrfaLΔarnT and ΔlonΔrfaLΔpagL, respectively. …”
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Phosphorylation of S‐S‐S Motif in Nuclear Export Protein (NEP) Plays a Critical Role in Viral Ribonucleoprotein (vRNP) Nuclear Export of Influenza A and B Viruses by Xiaokun Liu, Cha Yang, Xian Lin, Xiaomei Sun, Huanchun Chen, Qiang Zhang, Meilin Jin

“…However, neither the inhibitors above nor the NEP peptide mimics significantly inhibit the replication of H5N6‐DDD mutant, indicating phosphorylation of S‐S‐S motif is required for the vRNP nuclear export. …”
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