Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish by Feng Zhao, Guoying Ding, Qilong Wang, Huihui Du, Guosheng Xiao, Deqing Zhou

“…Thus, we constructed a waaf gene deletion mutant (i.e., △waaf) of wild-type (WT) which isolated from shellfish serotype O3:K6 via chitin-mediated transformation technology. …”
Get full text

Beclin 1-Mediated Autophagy Is Potentiated by an Interaction with the Neuronal Adaptor FE65 by Wai Wa Ray Chan, Jessica Chow, Dennis Dik-Long Chau, Yuqi Zhai, Kwok-Fai Lau

“…Moreover, the stimulatory effect of FE65 on Beclin 1-mediated autophagy is diminished by an FE65 C-terminus deletion mutant that disrupts the FE65–Beclin 1 interaction. …”
Get full text

Acidovorax temperans skews neutrophil maturation and polarizes Th17 cells to promote lung adenocarcinoma development by Joshua K. Stone, Natalia von Muhlinen, Chenran Zhang, Ana I. Robles, Amy L. Flis, Eleazar Vega-Valle, Akihiko Miyanaga, Masaru Matsumoto, K. Leigh Greathouse, Tomer Cooks, Giorgio Trinchieri, Curtis C. Harris

“…Here, we instilled A. temperans in an animal model driven by mutant K-ras and Tp53. This revealed A. temperans accelerates tumor development and burden through infiltration of proinflammatory cells. …”
Get full text

Dual RNA sequencing of a co-culture model of Pseudomonas aeruginosa and human 2D upper airway organoids by Cayetano Pleguezuelos-Manzano, Wouter A. G. Beenker, Gijs J. F. van Son, Harry Begthel, Gimano D. Amatngalim, Jeffrey M. Beekman, Hans Clevers, Jeroen den Hertog

“…Using dual RNA-sequencing, we dissected the interaction between organoid epithelial cells and WT or QS-mutant P. aeruginosa strains. Since only a single healthy individual and a single CF subject were used as donors for the organoids, conclusions about CF-specific effects could not be deduced. …”
Get full text

Genome-wide identification and expression analysis of GRAS transcription factors under cold stress in diploid and triploid Eucalyptus by Jiannan Liu, Hao Chen, Chenhe Li, Kang Du, Jun Yang

“…The GRAS [GRI (Gibberellic Acid Insensitive), RGA (Repressor of GAI-3 mutant), and SCR (Scarecrow)] transcription factors play a pivotal role in the development and stress responses of plants. …”
Get full text

Current Status of Treatment of Spinal and Bulbar Muscular Atrophy by Fumiaki Tanaka, Masahisa Katsuno, Haruhiko Banno, Keisuke Suzuki, Hiroaki Adachi, Gen Sobue

“…Since ligand-induced nuclear translocation of mutant AR has been shown to be a critical step in motor neuron degeneration in SBMA, androgen deprivation therapies using leuprorelin and dutasteride have been developed and translated into clinical trials. …”
Get full text

Characterisation of the antibody-mediated selective pressure driving intra-host evolution of SARS-CoV-2 in prolonged infection. by Michael Schoefbaenker, Theresa Günther, Eva Ulla Lorentzen, Marie-Luise Romberg, Marc Tim Hennies, Rieke Neddermeyer, Marlin Maybrit Müller, Alexander Mellmann, Chiara Robin Bojarzyn, Georg Lenz, Matthias Stelljes, Eike Roman Hrincius, Richard Vollenberg, Stephan Ludwig, Phil-Robin Tepasse, Joachim Ewald Kühn

“…Notably, the composition and frequency of escape mutations changed in a short period with an unprecedented dynamic. The triple mutant S:Delta141-4 E484K S494P became dominant until virus elimination. …”
Get full text

Porphyromonas gingivalis affects neutrophil pro-inflammatory activities by Agnieszka Zimny, Alicja Płonczyńska, Alicja Płonczyńska, Wiktor Jakubowski, Natalia Zubrzycka, Natalia Zubrzycka, Jan Potempa, Jan Potempa, Maja Sochalska

“…To investigate the impact of gingipains on neutrophil viability and function, we conducted studies using murine HoxB8 neutrophils and primary human neutrophils infected with wild-type strains of Porphyromonas gingivalis (W83 and ATCC 33277), or a gingipains-null mutant with deleted gingipains encoding genes, or wild-type bacteria preincubated with specific gingipain inhibitors. …”
Get full text

Neuroligin-3 R451C induces gain-of-function gene expression in astroglia in an astroglia-enriched brain organoid model by Rui Dang, Mridul Dalmia, Ziyuan Ma, Mengmeng Jin, Kushal Aluru, Vincent R. Mirabella, Ava V. Papetti, Li Cai, Peng Jiang

“…Interestingly, our RNA sequencing data suggest that these mutant astroglia significantly upregulate pathways that support neural functions when compared to isogenic wild-type astroglia. …”
Get full text

Dysfunctional BCAA degradation triggers neuronal damage through disrupted AMPK-mitochondrial axis due to enhanced PP2Ac interaction by Shih-Cheng Wu, Yan-Jhen Chen, Shih-Han Su, Pai-Hsiang Fang, Rei-Wen Liu, Hui-Ying Tsai, Yen-Jui Chang, Hsing-Han Li, Jian-Chiuan Li, Chun-Hong Chen

“…We investigated this by generating Drosophila mutants lacking BCAA-catabolic activity, resulting in elevated BCAA levels and neurological dysfunction, mimicking disease-relevant symptoms. …”
Get full text

Mitochondrial-ER Contact Sites and Tethers Influence the Biosynthesis and Function of Coenzyme Q by Noelle Alexa Novales, Hadar Meyer, Yeynit Asraf, Maya Schuldiner, Catherine F. Clarke

“…Here we show that deletion of COQ11 suppresses the respiratory deficient phenotype of select ERMES mutants, results in repair and reorganization of the CoQ synthome, and enhances mitochondrial CoQ domains. …”
Get full text

SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence in Streptococcus pneumoniae D39 by Ye Tao, Li Lei, Shuhui Wang, Xuemei Zhang, Yibing Yin, Yuqiang Zheng

“…Our results showed that the D39Δspd0410 mutant strain exhibited significantly elevated CPS levels compared to the parental strain D39s. …”
Get full text

Programmed neurite degeneration in human central nervous system neurons driven by changes in NAD+ metabolism by Markus Brüll, Selina Multrus, Michael Schäfer, Ivana Celardo, Christiaan Karreman, Marcel Leist

“…As a third approach to investigate the role of altered NAD+ metabolism, we made use of the WLD(s) protein, which has been found in a mutant mouse to inhibit Wallerian degeneration of axons. …”
Get full text

In Silico Analysis Revealed Five Novel High-Risk Single-Nucleotide Polymorphisms (rs200384291, rs201163886, rs193141883, rs201139487, and rs201723157) in ELANE Gene Causing Autosom... by Khyber Shinwari, Mikhail A. Bolkov, Muhammad Yasir Akbar, Liu Guojun, Svetlana S. Deryabina, Irina A. Tuzankina, Valery. A. Chereshnev

“…We identified the deleterious nsSNPs, constructed mutant protein structures, and evaluated the impact of mutation by employing molecular docking. …”
Get full text

A detoxified TLR4 agonist inhibits tumour growth and lung metastasis of osteosarcoma by promoting CD8+ cytotoxic lymphocyte infiltration by Ryunosuke Oyama, Akira Nabeshima, Makoto Endo, Alexey Novikov, Toshifumi Fujiwara, Capucine Phelip, Nobuhiko Yokoyama, Yoshinao Oda, Martine Caroff, Yoshihiro Matsumoto, Jerome Kerzerho, Yasuharu Nakashima

“…We evaluated tumour growth, lung metastases, and immune cell infiltration in wild-type and TLR4-mutant mice and performed selective immunodepletion. …”
Get full text

Multiomic quantification of the KRAS mutation dosage improves the preoperative prediction of survival and recurrence in patients with pancreatic ductal adenocarcinoma by Won-Gun Yun, Daeun Kim, Youngmin Han, Wooil Kwon, Seong-Geun Lee, Jin-Young Jang, Daechan Park

“…RNA, whole-exome, and KRAS-targeted sequencing data were used to estimate the dosage of the KRAS G12 mutant. Our prognostic scoring system included the mutation dosage from targeted sequencing ( > 0.195, 1 point), maximal tumor diameter at preoperative imaging ( > 20 mm, 1 point), and carbohydrate antigen 19-9 levels ( > 150 U/mL, 1 point). …”
Get full text

Structural insights into light-gating of potassium-selective channelrhodopsin by Takefumi Morizumi, Kyumhyuk Kim, Hai Li, Probal Nag, Tal Dogon, Oleg A. Sineshchekov, Yumei Wang, Leonid S. Brown, Songhwan Hwang, Han Sun, Ana-Nicoleta Bondar, Igor Schapiro, Elena G. Govorunova, John L. Spudich, Oliver P. Ernst

“…Here, we use single-particle cryo-electron microscopy of peptidisc-incorporated protein samples to determine the structures of the slow-cycling mutant C110A of kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) in the dark and upon laser flash excitation. …”
Get full text

Study on Management of Blood Transfusion Therapy in Patients with Hereditary Spherocytosis by Shiyue Ma, Lingjian Tang, Chaoli Wu, Hui Tang, Xue Pu, Jinhong Niu

“…SDS-PAGE results demonstrated that the concentration of band 3 was reduced in II-1, whereas the levels of the corresponding mutant proteins in the other probands were unchanged. …”
Get full text

Frequency and Prognostic Impact of CDKN2A/B Alteration in Oligodendrogliomas: Systematic Review and Meta-analysis by Satoshi NAKASU, Shoichi DEGUCHI, Yoko NAKASU

“…Isocitrate dehydrogenase (IDH)-mutant astrocytomas with homozygous deletion of cyclin-dependent kinase 2A/B (CDKN2A/B-HomoD) are categorized to grade 4 in the new World Health Organization (WHO) classification. …”
Get full text

Assessment of MGMT and TERT Subtypes and Prognosis of Glioblastoma by Whole Tumor Apparent Diffusion Coefficient Histogram Analysis by Ling Chen, Min Wu, Yao Li, Lifang Tang, Chuyun Tang, Lizhao Huang, Tao Li, Li Zhu

“…There were significant differences in ADCmin, ADCp10, ADCmean, and entropy between TERT promoter mutant and wild‐type groups. Entropy showed the best diagnostic performance in differentiating between positive and negative TERT groups (AUC = 0.722, p = 0.001). …”
Get full text