Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex by Gwang Sik Kim, Young Chul Lee

“…For the functional analysis of Saccharomyces cerevisiae Med6p (scMed6p), we generated a series of scMed6p mutants harboring a small internal deletion. Genetic analysis of these mutants revealed that three regions (amino acids 33–42 (Δ2), 125–134 (Δ5), and 157–166 (Δ6)) of scMed6p are required for cell viability and are located at highly conserved regions of Med6 homologs. …”
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A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approv... by Maryam Fothouhi, Peter S. McPherson, Carl Laflamme, Sara González Bolívar, Riham Ayoubi, Charles Alende, Irina Shlaifer

“…VAPB is an adaptor protein known for its role as an anchor for other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and the underlying mechanisms resulting from this defect are studied by researchers in this area to uncover its implication in the disease. …”
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YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress by Min Wang, Ping Feng, Xun Chen, Haifang Zhang, Bin Ni, Xiaofang Xie, Hong Du

“…In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. …”
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Effective in vivo binding energy landscape illustrates kinetic stability of RBPJ-DNA binding by Duyen Huynh, Philipp Hoffmeister, Tobias Friedrich, Kefan Zhang, Marek Bartkuhn, Francesca Ferrante, Benedetto Daniele Giaimo, Rhett A. Kovall, Tilman Borggrefe, Franz Oswald, J. Christof M. Gebhardt

“…We determine the binding kinetics, transcriptional activity, and genome-wide chromatin occupation of RBPJ and mutant variants by live-cell single-molecule tracking, reporter assays, and ChIP-Seq. …”
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Wheat NAC transcription factor NAC5‐1 is a positive regulator of senescence by Catherine Evans, Sophie Louise Mogg, Charline Soraru, Emma Wallington, Juliet Coates, Philippa Borrill

“…To characterize NAC5‐1, we combined missense mutations in NAC5‐A1 and NAC5‐B1 from a TILLING mutant population and overexpressed NAC5‐A1 in wheat. …”
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Interaction of toll-like receptors and ACE-2 with different variants of SARS-CoV-2: A computational analysis by Azadeh Zahmatkesh, Elham Salmasi, Reza Gholizadeh

“…Conclusion: It appears that Alpha and some Iranian isolates are the variants of concern due to their higher transmissibility and rapid spread. The Delta mutant is also a variant of concern, not only because of its closer interaction with ACE2, but also with TLR4. …”
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Regulation of stress granule maturation and dynamics by poly(ADP-ribose) interaction with PARP13 by Shang-Jung Cheng, Temitope Gafaar, Jijin R. A. Kuttiyatveetil, Aleksandr Sverzhinsky, Carla Chen, Minghui Xu, Allison Lilley, John M. Pascal, Anthony K. L. Leung

“…Furthermore, this PAR-binding mutant diminishes condensate dynamics and impedes fusion. …”
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The IQGAP-related RasGAP IqgC regulates cell–substratum adhesion in Dictyostelium discoideum by Lucija Mijanović, Darija Putar, Lucija Mimica, Sabina Klajn, Vedrana Filić, Igor Weber

“…Here, we show that the IQGAP-related protein IqgC is important for normal attachment of D. discoideum cells to the substratum. Mutant iqgC-null cells have impaired adhesion, whereas overexpression of IqgC promotes directional migration. …”
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Deconstructing the Politics of Linguistic Mutation in Tom Stoppard’s Dogg’s Hamlet, Cahoot’s Macbeth by Sarra Jouini

“…He stages a truncated, mutant version of Shakespeare’s plays where they are banned, giving voice to the intellectual restlessness of Czechoslovakia in the 1970s. …”
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Essential and recurrent roles for hairpin RNAs in silencing de novo sex chromosome conflict in Drosophila simulans. by Jeffrey Vedanayagam, Marion Herbette, Holly Mudgett, Ching-Jung Lin, Chun-Ming Lai, Caitlin McDonough-Goldstein, Stephen Dorus, Benjamin Loppin, Colin Meiklejohn, Raphaëlle Dubruille, Eric C Lai

“…Importantly, genetic interactions between nmy and tmy mutant alleles reveal that Tmy also specifically maintains male progeny for normal sex ratio. …”
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Determination of damaging SNP’s in SHANK3 gene with in silico methods by İrem Gülfem Albayrak, Şeyma Yektar, Süeda Kaya

“…Results This research uses in silico approaches such as SIFT, PolyPhen-2, I-Mutant 2.0, and Project HOPE to find harmful SNPs, which are the single nucleotide polymorphisms the most prevalent types of genetic variation in humans, in the SHANK3 gene. …”
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Rice glycosyltransferase OsDUGT1 is involved in heat stress tolerance by glycosylating flavonoids and regulating flavonoid metabolism by Guang-rui Dong, Shu-man Zhao, Yi Ding, Yu-qing Ma, Xing-mei Ma, Chong-lin Liu, Bing-kai Hou

“…Additionally, our transcriptomic analysis of Osdugt1 mutant lines under heat stress condition indicated that mutation of OsDUGT1 can reduce the transcriptional activity of heat response related genes, antioxidant enzyme genes and other genes involved in the flavonoid biosynthetic pathway. …”
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Association between BAFF gene polymorphism and systemic lupus erythematosus: a meta-analysis by Qing Wu, Qin Zhang, Dong-Qing Ye

“…Conclusion The correlation between BAFF gene promoter polymorphism-871C > T allele frequency and mutant genotype T and SLE risk was demonstrated, and a stronger correlation was found in the population subgroup analysis compared with non-Asians.…”
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Xanthomonas citri subsp. citri requires a polyketide cyclase to activate the type III secretion system for virulence by Shuying Zhu, Siyu Wu, Yanmin Liu, Zaibao Zhang, Huasong Zou

“…Furthermore, there as a remarkable reduction in the expression of type III genes, such as hrpG and hrpX. In the mutant carrying the pkc deletion, ketoleucine and acetone cyanohydrin were downregulated, and four metabolites, including d-ribose, creatine, polyoxyethylene dioleate, and cohibin C, were upregulated. …”
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Bilateral Pheochromocytomas in a Patient with Y175C Von Hippel-Lindau Mutation by Olga Astapova, Anindita Biswas, Alessandra DiMauro, Jacob Moalem, Stephen R. Hammes

“…VHL protein acts as a tumor suppressor by targeting hypoxia-inducible factors (HIFs) for degradation through an oxygen-dependent mechanism. VHL mutants with more severely reduced HIF degrading function carry a high risk of RCC, while mutants with preserved HIF degrading capacity do not cause RCC but still lead to other tumors. …”
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CBL mutations in chronic myelomonocytic leukemia often occur in the RING domain with multiple subclones per patient: Implications for targeting. by Kelly Lim, Winnie L Kan, Pramod C Nair, Monika Kutyna, Angel F Lopez, Timothy Hercus, David M Ross, Steven Lane, Chun Yew Fong, Anna Brown, Agnes Yong, David Yeung, Timothy Hughes, Devendra Hiwase, Daniel Thomas

“…Furthermore, we noted an increased percentage of CMML CD34+ stem and progenitor cells expressing CD116 and CD131 in all CBL mutant cases and increased CD116 receptor density compared to healthy controls, similar to CMML overall. …”
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The role of the pAbp gene encoding the cytoplasmic poly(A)-binding protein in spermatogenesis of Drosophila melanogaster by E. U. Bolobolova, E. M. Baricheva, N. V. Dorogova

“…However, some flies carrying combinations of mutant pAbp alleles survive but display male sterility and show defects in spermatogenesis. …”
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Temperature Depended Role of Shigella flexneri Invasion Plasmid on the Interaction with Acanthamoeba castellanii by Amir Saeed, David Johansson, Gunnar Sandström, Hadi Abd

“…A. castellanii in the absence or presence of wild type, IpaB mutant, or plasmid-cured strain S. flexneri was cultured at 30∘C and 37∘C and the interaction was analysed by viable count of both bacteria and amoebae, electron microscopy, flow cytometry, and statistical analysis. …”
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Boosting the human antiviral response in conjunction with natural plant products by Rashmi Srivastava, Neeraj Kumar Dubey, Megha Sharma, Harsha Kharkwal, Rajesh Bajpai, Rakesh Srivastava

“…The increasing prevalence of viral infections and the emergence of drug-resistant or mutant strains necessitate the exploration of novel antiviral strategies. …”
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Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran by Danial Jahantigh, Saeedeh Salimi, Roya Alavi-Naini, Abolfazl Emamdadi, Hamid Owaysee Osquee, Farzaneh Farajian Mashhadi

“…The frequencies of the mutant alleles of TLR4 Arg299Gly, Thr399Ile, and TLR9 T-1486C polymorphisms were 0.8 versus 0.1, 5.6 versus 3, and 28.6 versus 25.2 in patients and controls, respectively, that were not significant. …”
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