Spectral scanning and fluorescence lifetime imaging microscopy (FLIM) enable separation and characterization of C. elegans autofluorescence in the cuticle and gut by Heino J. Hulsey-Vincent, Elizabeth A. Cameron, Caroline L. Dahlberg, Domenico F. Galati

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Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy by Pillai Arun Gopinathan, Ganganna Kokila, Mahadesh Jyothi, Chatterjee Ananjan, Linganna Pradeep, Salroo Humaira Nazir

“…The present study was done to evaluate birefringence pattern of collagen fibres in different grades of oral squamous cell carcinoma using Picrosirius red stain and polarization microscopy and to determine if there is a change in collagen fibres between different grades of oral squamous cell carcinoma. …”
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Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction by Marco Tobia Vitali, Alessia Candeo, Andrea Farina, Paolo Pozzi, Alessia Brix, Andrea Bassi, Teresa M Correia

“…Light Sheet Fluorescence Microscopy (LSFM) enables rapid and gentle 3D fluorescence imaging of dynamic processes over extended periods in translucent samples at the mesoscopic scale. …”
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Disentangling the effects of intermittent faecal shedding and imperfect test sensitivity on the microscopy-based detection of gut parasites in stool samples. by Lana C E Ferreira-Sá, Eleuza R Machado, Rodrigo Gurgel-Gonçalves, Fernando Abad-Franch

“…Samples (413 overall) were independently examined, via standard sedimentation/optical microscopy, by a senior parasitologist and a junior, trained student (826 tests overall). …”
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Large-angle Lorentz Four-dimensional scanning transmission electron microscopy for simultaneous local magnetization, strain and structure mapping by Sangjun Kang, Maximilian Töllner, Di Wang, Christian Minnert, Karsten Durst, Arnaud Caron, Rafal E. Dunin-Borkowski, Jeffrey McCord, Christian Kübel, Xiaoke Mu

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FIRM image analysis: A machine learning workflow for quantifying extracellular matrix components from electron microscopy images. by Nicholas T Gigliotti, Justin Lee, Emily H Mang, Giancarlo R Zambrano, Mitra L Taheri

“…Although image analysis has seen much improvement in recent years, there has been no technique developed to address ambiguity in feature edges in electron microscopy images. Presented here is a new machine learning-based workflow for the analysis of microscopy images named FIRM (Feature Identification from Raw Microscopy) that uses a random forest classifier to identify ECM features of interest and generate binary segmentation masks for quantification with ImageJ-FIJI. …”
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System- and sample-agnostic isotropic three-dimensional microscopy by weakly physics-informed, domain-shift-resistant axial deblurring by Jiashu Han, Kunzan Liu, Keith B. Isaacson, Kristina Monakhova, Linda G. Griffith, Sixian You

“…Abstract Three-dimensional subcellular imaging is essential for biomedical research, but the diffraction limit of optical microscopy compromises axial resolution, hindering accurate three-dimensional structural analysis. …”
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Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view by Zhenguo Wu, Yunxian Tian, Jianhua Zhao, Yimei Huang, Harvey Lui, Sunil Kalia, Haishan Zeng

“…Traditionally high-resolution in vivo skin microscopy methods capture images in the en-face (xy) plane parallel to the skin surface but are affected by involuntary motion, particularly during large-area volumetric data acquisition using xy-z mosaicking. …”
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Cost–Benefit Analysis of in vivo Reflectance Confocal Microscopy for Melanoma Diagnosis in a Real-World Clinical Setting by Bruno GM, Di Matteo S, Longo C, Stanganelli I, Farnetani F, Borsari S, Mazzoni L, Ciardo S, Raucci M, Magi S, Bassoli S, Spadafora M, Mandel VD, Casari A, Chester J, Kaleci S, Colombo GL, Pellacani G

Subjects: “…reflectance confocal microscopy…”
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Histopathologic evaluation of wooden breast and white striping myopathy in different broiler genotypes using light microscopy and image analysis by Zehra Avci-Kupeli, Erdem Caglar-Kupeli, Metin Petek

“…Additionally, histopathological lesions such as lipidosis, inflammatory cell infiltration, connective tissue formation, and degeneration were compared among genotypes using manual scoring with traditional light microscopy, as well as less commonly utilized digital image analysis software, including ImageJ and QuPath. …”
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The Impact of Carbon on Electronic Structure of N-Doped ZnO Films: Scanning Photoelectron Microscopy Study and DFT Calculations by Elzbieta Guziewicz, Sushma Mishra, Matteo Amati, Luca Gregoratti, Oksana Volnianska

“…A Scanning Photoelectron Microscopy (SPEM) experiment has been applied to ZnO:N films deposited by Atomic Layer Deposition (ALD) under O-rich conditions and post-growth annealed in oxygen at 800 °C. …”
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Minimal differences observed when comparing the morphological profiling of microglia obtained by confocal laser scanning and optical sectioning microscopy by Sânziana Godeanu, Sânziana Godeanu, Mădălina Iuliana Mușat, Anja Scheller, Anja Scheller, Eugen Osiac, Bogdan Cătălin, Bogdan Cătălin

Subjects: “…confocal laser scanning microscopy…”
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Identification and Quality Evaluation of Raw and Processed Asarum Species Using Microscopy, DNA Barcoding, and Gas Chromatography-Mass Spectrometry by Guangzhe Yao, Wenjuan Ma, Xuhua Huang, Qi Jia, Jiayuan Shen, Yanxu Chang, Huizi Ouyang, Jun He

“…In this study, 16 batches of Asarum herbs were collected, and their species were identified using DNA barcoding, which is a method for distinguishing plant species, coupled with microscopy. A gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination of 10 compounds was established to evaluate the contents of raw and processed Asarum herbs. …”
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Effect of Annealing Temperature on the Morphology and Piezoresponse Characterisation of Poly(vinylidene fluoride-trifluoroethylene) Films via Scanning Probe Microscopy by K. Lau, Y. Liu, H. Chen, R. L. Withers

“…Ferroelectric switchable domains were identified by piezoresponse force microscopy (PFM) for all films. Small grains, with weak piezoresponse character, were observed for films annealed just above the Curie temperature. …”
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Lens-Free On-Chip Quantitative Phase Microscopy for Large Phase Objects Based on a Biplane Phase Retrieval Method by Yufan Chen, Xuejuan Wu, Yang Chen, Wenhui Lin, Haojie Gu, Yuzhen Zhang, Chao Zuo

Subjects: “…lens-free on-chip microscopy…”
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Cation-Specific Effects on the Self-Assembly of Collagen Molecules Mediated by Acetate on Mica Surface Observed with Atomic Force Microscopy by Zhiwei Wang, Qi Xiao, Xuan Song, Yunfei Wan, Jie Zhu

“…In this work, collagen extracted from fish scales by acid-base method was used to explore the self-assembly characters, and atomic force microscopy was applied to observe the collagen assembled on mica surface mediated by acetate with four different cations, including K+, Na+, Mg2+, and Ca2+. …”
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Microscopic methods in analysis of submicron phospholipid dispersions by Płaczek Marcin, Kosela Magdalena

Subjects: “…optical microscopy…”
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Application of IR Fourier microscopy for investigation of kimberlite indicator minerals to search for diamond deposits using heavy-mineral concentrate method by Khachatryan G. K., Anashkina N. E.

“…Possibilities of the infrared microscopy technique as applied to the express diagnostics of diamond crystals and of grains of most of its satellite minerals, extracted from heavy mineral concentrate (HMC) samples, are considered. …”
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In Vivo Corneal Confocal Microscopy Detects Improvement of Corneal Nerve Parameters following Glycemic Control in Patients with Type 2 Diabetes by Xiaofan Jia, Xiaogang Wang, Xiaoxia Wang, Qi Pan, Tongzhang Xian, Xiaobin Yu, Lixin Guo

“…This study aimed to investigate whether in vivo corneal confocal microscopy (CCM) can detect the improvement of corneal nerve parameters following glycemic control in patients with type 2 diabetes in natural history. …”
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Unsupervised inter-domain transformation for virtually stained high-resolution mid-infrared photoacoustic microscopy using explainable deep learning by Eunwoo Park, Sampa Misra, Dong Gyu Hwang, Chiho Yoon, Joongho Ahn, Donggyu Kim, Jinah Jang, Chulhong Kim

“…Abstract Mid-infrared photoacoustic microscopy can capture biochemical information without staining. …”
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