Optimizing Imaging of Three-Dimensional Multicellular Tumor Spheroids with Fluorescent Reporter Proteins Using Confocal Microscopy by Lucia le Roux, Andrei Volgin, David Maxwell, Katashi Ishihara, Juri Gelovani, Dawid Schellingerhout

“…In this article, we show a series of optimizations that allowed us to obtain excellent quality confocal microscopy images at imaging depths of up to 320 μm. …”
Get full text

Surface Precision in Orthodontics: A Comprehensive Analysis of Interproximal Reduction Methods Using Atomic Force Microscopy by Abhishek Sinha, Sneha Surendran, Rashme Rashme, Savadamoorthi Kamatchi Subramani

Subjects: “…atomic force microscopy…”
Get full text

Multiscale characterization of medical devices and pharmaceutical formulations with 3D X-ray microscopy and computed tomography by Herminso Villarraga-Gómez, Ria L. Mitchell

“…It also covers deep-learning (DL) data reconstruction techniques that enhance 3D X-ray microscopy (XRM) and computed tomography (CT) applications in drug delivery and the pharmaceutical industry. …”
Get full text

Laboratory-based x-ray phase contrast microscopy system for targeting in unstained soft-tissue samples by Michela Esposito, Nicole Schieber, Alessandro Olivo, Yannick Schwab, Marco Endrizzi

“…Its potential for soft-tissue microscopy is demonstrated on two biological specimens and benchmarked against gold-standard synchrotron data. …”
Get full text

Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy by Takeo Minamikawa, Yoshinori Murakami, Naokazu Matsumura, Hirohiko Niioka, Shuichiro Fukushima, Tsutomu Araki, Mamoru Hashimoto

“…Our findings suggested that the multifocus excitation scheme is expected to be suitable for CARS microscopy in terms of minimal invasiveness.…”
Get full text

Mechanical testing of miniature carbon fiber reinforced polymer (CFRP) samples under digital light microscopy by E. S. Statnik, Iu. A. Sadykova, E. N. Prokopev, A.I. Salimon, E. V. Nazarov, N. V. Turbin, A.M. Korsunsky

Subjects: Get full text

Accelerating biopharmaceutical cell line selection with label-free multimodal nonlinear optical microscopy and machine learning by Jindou Shi, Alexander Ho, Corey E. Snyder, Eric J. Chaney, Janet E. Sorrells, Aneesh Alex, Remben Talaban, Darold R. Spillman, Marina Marjanovic, Minh Doan, Gary Finka, Steve R. Hood, Stephen A. Boppart

“…Employing simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy with fluorescence lifetime imaging microscopy (FLIM), we characterized Chinese hamster ovary (CHO) cell lines at early passages (0–2). …”
Get full text

A 2D cell segmentation protocol for monitoring multiple STAT signaling pathways by fluorescence microscopy by Gergő Szanda, Éva Wisniewski, László Barna, Gábor Turu, Ken Mackie

Subjects: “…Microscopy…”
Get full text

Conventional and High-Speed Intravital Multiphoton Laser Scanning Microscopy of Microvasculature, Lymphatics, and Leukocyte-Endothelial Interactions by Timothy P. Padera, Brian R. Stoll, Peter T.C. So, Rakesh K. Jain

“…Multiphoton laser scanning microscopy (MPLSM) has recently been applied in vivo to overcome three drawbacks associated with traditional epifluorescence microscopy: (i) limited depth of imaging due to scattering of excitation and emission light; (ii) projection of three-dimensional structures onto a two-dimensional plane; and (iii) phototoxicity. …”
Get full text

In Vitro Whole Blood Clot Lysis for Fibrinolytic Activity Study Using D-Dimer and Confocal Microscopy by Abuzar Elnager, Wan Zaidah Abdullah, Rosline Hassan, Zamzuri Idris, Nadiah Wan Arfah, S. A. Sulaiman, Zulkifli Mustafa

“…The fibrinolytic activity was assessed by D-dimer (DD), confocal microscopy, and clot weight. DD was measured photometrically by immunoturbidimetric method. …”
Get full text

Effect of Chronic Morphine Consumption on Synaptic Plasticity of Rat’s Hippocampus: A Transmission Electron Microscopy Study by Mohammad Hassan Heidari, Abdollah Amini, Zohreh Bahrami, Ali Shahriari, Abolfazle Movafag, Reihane Heidari

Get full text

Analysis of a Cementless Femoral Stem Neck Fracture Using Scanning Electron Microscopy and the Finite Element Method by Hirokazu Takai, Daisuke Nakayama, Masatoshi Murayama, Tomoki Takahashi

“…We examined the broken stem by digital microscopy, scanning electron microscopy, and finite element method. …”
Get full text

MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain by Ismael Benito-Altamirano, Sergio Moreno, David M. Vaz-Romero, Anna Puig-Pujol, Gemma Roca-Domènech, Joan Canals, Anna Vilà, Joan Daniel Prades, Ángel Diéguez

Subjects: Get full text

Detection of Viable but Nonculturable E. coli Induced by Low-Level Antimicrobials Using AI-Enabled Hyperspectral Microscopy by MeiLi Papa, Aarham Wasit, Justin Pecora, Teresa M. Bergholz, Jiyoon Yi

Subjects: Get full text

Quantifying glucose uptake at the single cell level with confocal microscopy reveals significant variability within and across individuals by Joel D. Paprocki, Patrick J. Macdonald, Yongjin Xu, Alan Cheng, Timothy C. Dunn, Sergey Y. Tetin

“…Here, we devised a fluorescence-based method to quantitatively observe GLUT1-mediated intracellular glucose analog tracer uptake in individual RBCs utilizing microfluidics and confocal microscopy. For the first time, we demonstrate that intracellular/extracellular tracer percentages can be measured at the single cell level using the fluorescently labeled glucose analog, 2-NBDG. …”
Get full text

New findings of dermoscopy and reflectance confocal microscopy for perforating pseudoxanthoma elasticum with elastosis perforans serpiginosa-like changes by Juan Zhao, PhD, Ruiya Li, MD, Hongyong Sun, MD, Yan Duan, PhD

Subjects: “…reflectance confocal microscopy…”
Get full text

Characterization of Precipitation in Al-Li Alloy AA2195 by means of Atom Probe Tomography and Transmission Electron Microscopy by Muna Khushaim, Torben Boll, Judith Seibert, Ferdinand Haider, Talaat Al-Kassab

“…The electrochemically prepared samples were analyzed by scanning transmission electron microscopy and atom probe tomography for chemical mapping. …”
Get full text

Magnetic Targeting of Rhodamine-Labeled Superparamagnetic Liposomes to Solid Tumors: In Vivo Tracking by Fibered Confocal Fluorescence Microscopy by Marie-Sophie Martina, Jean-Paul Fortin, Laure Fournier, Christine Ménager, Florence Gazeau, Olivier Clément, Sylviane Lesieur

“…Noninvasive fibered confocal fluorescence microscopy was successfully used to track the liposomes in vivo within organs and tumor blood vessels. …”
Get full text

Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy by Xuejiao Tian, Tzu-Yang Lin, Po-Ting Lin, Min-Ju Tsai, Hsin Chen, Wen-Jie Chen, Chia-Ming Lee, Chiao-Hui Tu, Jui-Cheng Hsu, Tung-Han Hsieh, Yi-Chung Tung, Chien-Kai Wang, Suewei Lin, Li-An Chu, Fan-Gang Tseng, Yi-Ping Hsueh, Chi-Hon Lee, Peilin Chen, Bi-Chang Chen

“…Abstract Taking advantage of the good mechanical strength of expanded Drosophila brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic fluorescent proteins preserved by glutaraldehyde fixation in the nervous system. …”
Get full text

High-resolution imaging of organic and inorganic nanoparticles at nanometre-scale resolution by X-ray ensemble diffraction microscopy by Ning-Jung Chen, Chia-Hui Yeh, Huai-Yu Cao, Nai-Chi Chen, Chun-Jung Chen, Chun-Yu Chen, Yi-Wei Tsai, Jhih-Min Lin, Yu-Shan Huang, Chien-Nan Hsiao, Chien-Chun Chen

Subjects: “…ensemble diffraction microscopy…”
Get full text