Physiological accumulation of lipid droplets in the newborn liver during breastfeeding is driven by TLR4 ligands by Wanderson Ferreira da Silva Júnior, Karen Marques de Oliveira Costa, Hortência Maciel Castro Oliveira, Maísa Mota Antunes, Kassiana Mafra, Brenda Naemi Nakagaki, Pedro Sérgio Corradi da Silva, Júlia Duarte Megale, Sarah Campos de Sales, Douglas Carvalho Caixeta, Mário Machado Martins, Robinson Sabino-Silva, Cristina Maria Pinto de Paula, Luiz Ricardo Goulart, Rafael Machado Rezende, Gustavo Batista Menezes

“…Using high-resolution microscopy assessed the spatial distribution of lipid droplets within hepatocytes. …”
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Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy by Timothy J Abreo, Emma C Thompson, Anuraag Madabushi, Kristen L Park, Heun Soh, Nissi Varghese, Carlos G Vanoye, Kristen Springer, Jim Johnson, Scotty Sims, Zhigang Ji, Ana G Chavez, Miranda J Jankovic, Bereket Habte, Aamir R Zuberi, Cathleen M Lutz, Zhao Wang, Vaishnav Krishnan, Lisa Dudler, Stephanie Einsele-Scholz, Jeffrey L Noebels, Alfred L George, Atul Maheshwari, Anastasios Tzingounis, Edward C Cooper

“…Analyzing prior KCNQ2 channel cryoelectron microscopy models revealed G256 as a node of an arch-shaped non-covalent bond network linking S5, the pore turret, and the ion path. …”
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Surface prereacted glass-ionomer particles incorporated into resin composites promote biocompatibility for restoration of subgingival dental defects by Yueyi Yang, Jing Huang, Xuchen Hu, Meiling Jing, Yujie Zhang, Chenci Xu, Wenduo Tan, Xiaoyu Liu, Chenguang Niu, Zhengwei Huang

“…The microstructure and elemental distribution were characterized by scanning electronic microscopy and energy-dispersive spectroscopy. The resin composites containing 50 or 70 wt% S-PRG fillers exhibited comprehensively better physicochemical properties, including flexural modulus, compressive strength, and water sorption. …”
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Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations by Lucas Tricoli, Sunetra Sase, Julia L. Hacker, Vi Pham, Maxwell Chappell, Laura Breda, Stephanie N. Hurwitz, Naoto Tanaka, Carlo Castruccio Castracani, Amaliris Guerra, Zhongqi Hou, Lars Schlotawa, Karthikeyan Radhakrishnan, Matthew Hogenauer, Aoife Roche, John Everett, Frederic Bushman, Peter Kurre, Rebecca Ahrens-Nicklas, Laura A. Adang, Adeline L. Vanderver, Stefano Rivella

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Genes Induced by Panax Notoginseng in a Rodent Model of Ischemia-Reperfusion Injury by Lanqing Meng, Qing Huang, Xuebin Li, Ping Liang, Yueyong Li, Xiaohua Huang, Jingjie Zhao, Qiuping Chen, Rong Qiu, Lan Li, Chongdong Jian, Hongfei Yao, Jianmin Huang, Xionglin Tang, Zechen Wang, Zhongheng Wei, Jun Wu, Liuzhi Wei, Qiuju Wei, Qianli Tang, Lu Huang, Jihua Wei, Dinggui Lu, Qunqiang Luo, Kegong Xie, Yang Ouyang, Jian Chen, Genliang Li, Linxue Luo, Linbo He, Chenyi Zhuo, Anding Xu, Lingzhang Meng

“…After that, staining with 2,3,5-triphenyltetrazolium chloride was employed to evaluate the infarcted area, and electron microscopy was used to assess ultrastructural changes of the neurovascular unit. …”
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Characterisation of <i>Staphylococcus aureus</i> Strains and Their Prophages That Carry Horse-Specific Leukocidin Genes <i>lukP/Q</i> by Stefan Monecke, Sindy Burgold-Voigt, Andrea T. Feßler, Martina Krapf, Igor Loncaric, Elisabeth M. Liebler-Tenorio, Sascha D. Braun, Celia Diezel, Elke Müller, Martin Reinicke, Annett Reissig, Adriana Cabal Rosel, Werner Ruppitsch, Helmut Hotzel, Dennis Hanke, Christiane Cuny, Wolfgang Witte, Stefan Schwarz, Ralf Ehricht

“…In addition to sequencing, phages were characterised by mitomycin C induction and transmission electron microscopy (TEM). All <i>lukP/Q</i> prophages integrated into the <i>lip2</i>=<i>geh</i> gene, and all included also the gene <i>scn-eq</i> encoding an equine staphylococcal complement inhibitor. …”
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Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88 by Hongbo Yi, Li Wang, Yunxia Xiong, Zhilin Wang, Yueqin Qiu, Xiaolu Wen, Zongyong Jiang, Xuefen Yang, Xianyong Ma

“…Using colloidal gold immunoelectron microscopy, these effects of L. reuteri LR1 on ZO-1 and occludin content in IPEC-1 cells were confirmed. …”
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Non-lethal sonodynamic therapy mitigates hypertensive renal fibrosis through the PI3K/AKT/mTORC1-autophagy pathway by DanDan Liu, Hui Wang, Jialong Li, Siqi Sheng, Shu Wang, Ye Tian

“…Pathway-related protein inhibitors (the autophagy-related inhibitor 3-methyladenine (3-MA), chloroquine (CQ), ROS inhibitor N-acetyl-L-cysteine (NAC) were applied, and autophagosome changes were observed under transmission electron microscopy. Immunofluorescence was used to observe LC3 spot formation within cells. …”
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ApoB100 remodeling and stiffened cholesteryl ester core raise LDL aggregation in familial hypercholesterolemia patients by Maria Teresa La Chica Lhoëst, Andrea Martínez, Eduardo Garcia, Jany Dandurand, Anna Polishchuk, Aleyda Benitez-Amaro, Ana Cenarro, Fernando Civeira, Amable Bernabé, David Vilades, Joan Carles Escolà-Gil, Valerie Samouillan, Vicenta Llorente-Cortes

“…Dynamic light scattering and transmission electron microscopy showed that LDL in FH patients exhibited smaller size and greater susceptibility to aggregation. …”
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N6-methyladenosine RNA modification regulates the transcription of SLC7A11 through KDM6B and GATA3 to modulate ferroptosis by Haisheng Zhang, Cheng Yi, Jianing Li, Yunqing Lu, Haoran Wang, Lijun Tao, Jiawang Zhou, Yonghuang Tan, Jiexin Li, Zhuojia Chen, Gholamreza Asadikaram, Jie Cao, Jianxin Peng, Wanglin Li, Junming He, Hongsheng Wang

“…Transmission electron microscopy and flow cytometry were used to detect mitochondrial reactive oxygen species (ROS). …”
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Evaluation of dried blood spot sampling for real-time PCR malaria diagnostics in a rural setting in Angola by Alejandro Mediavilla, Begoña Febrer-Sendra, Aroa Silgado, Patricia Martínez-Vallejo, Beatriz Crego-Vicente, Arlette Nindia, Carles Rubio Maturana, Lidia Goterris, Joan Martínez-Campreciós, Sandra Aixut, Pedro Fernández-Soto, María Luisa Aznar, Antonio Muro, Inés Oliveira-Souto, Israel Molina, Elena Sulleiro

“…(ii) Field diagnosis (microscopy and/or RDT) showed a 51.5% (103/200) positivity rate, while 50% (100/200) of the DBS samples tested positive by real-time PCR. …”
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Protective mechanism of safflower yellow injection on myocardial ischemia-reperfusion injury in rats by activating NLRP3 inflammasome by Lingmei Li, Ce Cao, Hao Guo, Li Lin, Lei Li, Yehao Zhang, Gaojie Xin, Zixin Liu, Shujuan Xu, Xiao Han, Qiong Zhang, Jianhua Fu

“…Cell morphology and cell viability were detected by transmission electron microscopy and CCK-8. Results In vivo, compared with sham group, the percentage of myocardial infarction area was increased and myocardial tissue arrangement was disordered in I/R group. …”
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Collagen deposition in lung parenchyma driven by depletion of interstitial Lyve-1+ macrophages prevents cigarette smoke-induced emphysema and loss of airway function by Yinebeb Mezgebu Dagnachew, Yinebeb Mezgebu Dagnachew, Yinebeb Mezgebu Dagnachew, Hwee Ying Lim, Hwee Ying Lim, Liao Wupeng, Liao Wupeng, Sheau Yng Lim, Sheau Yng Lim, Sheng Jie Natalie Lim, Sheng Jie Natalie Lim, Chung Hwee Thiam, Chung Hwee Thiam, Shu Wen Tan, Shu Wen Tan, Joan Lau Joo Eng, Joan Lau Joo Eng, Dan Mei, Syaza Hazwany Mohammad Azhar, Wei Siong Ong, Qi Hui Caris Tan, Wai-Shiu Fred Wong, Wai-Shiu Fred Wong, Wai-Shiu Fred Wong, Veronique Angeli, Veronique Angeli

“…Further analysis by polarized light microscopy and Sircol collagen assay revealed that the collagen fibers accumulating in the lungs depleted of Lyve-1+ macrophages were thicker and crosslinked. …”
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MERS-CoV spike vaccine-induced N-terminal domain-specific antibodies are more protective than receptor binding domain-specific antibodies by Olubukola M. Abiona, Nianshuang Wang, Sarah R. Leist, Alexandra Schäfer, Adam S. Cockrell, Lingshu Wang, Sandhya Bangaru, Laura Stevens, Rachel L. Graham, Jacob F. Kocher, Yaroslav Tsybovsky, Masaru Kanekiyo, Azad Kumar, Kaitlyn M. Morabito, Osnat Rosen, Wei Shi, Anne Werner, Yi Zhang, Cynthia Ziwawo, Christian K.O. Dzuvor, Charis Palandjian, Connor Eastman, Hannah R. Matthews, Jeswin Joseph, James D. Chappell, Wing-Pui Kong, John R. Mascola, Andrew B. Ward, Mark R. Denison, Ralph Baric, Jason S. McLellan, Barney S. Graham, Kizzmekia S. Corbett-Helaire

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E. Coli cytotoxic necrotizing factor-1 promotes colorectal carcinogenesis by causing oxidative stress, DNA damage and intestinal permeability alteration by Michela Tozzi, Alessia Fiore, Sara Travaglione, Francesca Marcon, Gabriella Rainaldi, Elena Angela Pia Germinario, Ilenia Laterza, Simona Donati, Daniele Macchia, Massimo Spada, Omar Leoni, Maria Cristina Quattrini, Donatella Pietraforte, Sofia Tomasoni, Filippo Torrigiani, Ranieri Verin, Paola Matarrese, Lucrezia Gambardella, Francesca Spadaro, Maria Carollo, Agostina Pietrantoni, Francesca Carlini, Concetta Panebianco, Valerio Pazienza, Filomena Colella, Donatella Lucchetti, Alessandro Sgambato, Antonella Sistigu, Federica Moschella, Marco Guidotti, Olimpia Vincentini, Zaira Maroccia, Mauro Biffoni, Roberta De Angelis, Laura Bracci, Alessia Fabbri

“…Methods Viability assays, confocal microscopy of γH2AX and 53BP1 molecules and cytogenetic analysis were carried out to assess CNF1-induced genotoxicity on non-neoplastic intestinal epithelial cells. …”
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Contribution à l’histoire de la boulangerie romaine : étude de « pains/galettes » découverts en Gaule by Andreas G. Heiss, Véronique Matterne, Nicolas Monteix, Margaux Tillier, Camille Noûs

“…Examination of the surfaces, with the naked eye or by optical microscopy, in most cases does not reveal any identifiable macroscopic component; it is therefore necessary to use appropriate and non-destructive analytical methods. …”
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Uvaol attenuates TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cells by modulating expression and membrane localization of β-catenin by Liliane Patrícia Gonçalves Tenório, Liliane Patrícia Gonçalves Tenório, Liliane Patrícia Gonçalves Tenório, Liliane Patrícia Gonçalves Tenório, Liliane Patrícia Gonçalves Tenório, Felipe Henrique da Cunha Xavier, Felipe Henrique da Cunha Xavier, Felipe Henrique da Cunha Xavier, Felipe Henrique da Cunha Xavier, Mônica Silveira Wagner, Kayo Moreira Bagri, Erick Gabriel Alves Ferreira, Romulo Galvani, Romulo Galvani, Romulo Galvani, Romulo Galvani, Claudia Mermelstein, Adriana Cesar Bonomo, Adriana Cesar Bonomo, Adriana Cesar Bonomo, Adriana Cesar Bonomo, Wilson Savino, Wilson Savino, Wilson Savino, Wilson Savino, Emiliano Barreto, Emiliano Barreto

“…Changes in cell shape were measured using phase-contrast and confocal microscopy, whereas protein expression levels were measured using immunofluorescence, flow cytometry, and Western blotting. …”
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Hybridization-based discovery of novel quinazoline-2-indolinone derivatives as potent and selective PI3Kα inhibitors by Changqun Liu, Yuening Cao, Yi Zuo, Chaozheng Zhang, Senmiao Ren, Xin Zhang, Chuanqi Wang, Yingjie Zeng, Jie Ling, Yilan Liu, Zixian Chen, Xiujun Cao, Zhengzhi Wu, Chuantao Zhang, Jun Lu

“…The biological evaluation of compound 8 was performed by transwell, flow cytometry, laser scanning confocal microscopy, Western blot, CTESA and immunohistochemistry. …”
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Solar-Driven Water Purification: Advancing PVA-Chitosan/PANI Hydrogel to Enhance Solar Vapor Generation for Freshwater Treatment by Flora Serati, Syazwani Mohd Zaki, Ahmad Akid Zulkifli, Siti Rabizah Makhsin

“…The morphological structure of the obtained hydrogels was analyzed by scanning electron microscopy (SEM). In contrast, the physicochemical and mechanical properties of the hydrogels were evaluated by dynamic light scattering (DLS), FTIR, swelling test and rheology. …”
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AdipoRon ameliorates the progression of heart failure with preserved ejection fraction via mitigating lipid accumulation and fibrosis by Wuping Tan, Yijun Wang, Siyi Cheng, Zhihao Liu, Mengjie Xie, Lingpeng Song, Qinfang Qiu, Xiaofei Wang, Zeyan Li, Tianyuan Liu, Fuding Guo, Jun Wang, Xiaoya Zhou

“…Cardiac function was evaluated using echocardiography, and Postmortem analysis included RNA-sequencing, untargeted metabolomics, transmission electron microscopy and molecular biology methods. Results: Our study presents the pioneering evidence that AdipoR was downregulated and impaired fatty acid oxidation in the myocardia of HFpEF mice, which was associated with lipid metabolism as indicated by untargeted metabolomics. …”
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