Characteristics of the smallest brucellaphage with strong lytic ability by Hongbaiyu Liu, Hongbaiyu Liu, Youhong Zhong, Zhihong Zhang, Kehong Xu, Kehong Xu, Chunpeng Mao, Chunpeng Mao, Qiuju Yang, Lihua Yang, Binbin Yu, Ying Long, Ying Long, Xinyu Qin, Xinyu Qin, Liyuan Shi, Sheng Chang, Yuanying Shen, Peng Wang

“…Transmission electron microscopy revealed that Y17 was composed of an icosahedral head (48.1 ± 2 nm) and a short tail (10.8 ± 1 nm), making it the smallest brucellaphage described so far. …”
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Analysis of stomatal characteristics of maize hybrids and their parental inbred lines during critical reproductive periods by Changyu Zhang, Changyu Zhang, Yu Jin, Yu Jin, Jinglu Wang, Jinglu Wang, Ying Zhang, Ying Zhang, Yanxin Zhao, Xianju Lu, Xianju Lu, Wei Song, Xinyu Guo, Xinyu Guo

“…This study employed Jingnongke 728 and its parental inbred to capture stomatal images from various leaf positions and abaxial surfaces during key reproductive stages using rapid scanning electron microscopy. We uesd a target detection and image segmentation approach based on YOLOv5s and Unet to efficiently obtain 11 phenotypic traits encompassing stomatal count, shape, and distribution. …”
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Annexin A8 deficiency delays atherosclerosis progression by Carmen Gutiérrez‐Muñoz, Rafael Blázquez‐Serra, Irene San Sebastian‐Jaraba, Sandra Sanz‐Andrea, Maria J. Fernández‐Gómez, Gonzalo Nuñez‐Moreno, Pablo Mínguez, Joan Carles Escolá‐Gil, Paula Nogales, Veronique Ollivier, Jose L. Martín‐Ventura, Benoit Ho‐Tin Noe, Ursula Rescher, Nerea Méndez‐Barbero, Luis M. Blanco‐Colio

“…In vitro experiments revealed that AnxA8 deficiency in MAECs suppressed P/E‐selectin and CD31 expression and secretion induced by ox‐LDL with a concomitant reduction in platelet and leukocyte adhesion. Intravital microscopy confirmed the reduction in leukocyte and platelet adhesion in ApoE−/−AnxA8−/− mice. …”
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Automated Quantification of Neuropad Improves Its Diagnostic Ability in Patients with Diabetic Neuropathy by Georgios Ponirakis, Hassan Fadavi, Ioannis N. Petropoulos, Shazli Azmi, Maryam Ferdousi, Mohammad A. Dabbah, Ahmad Kheyami, Uazman Alam, Omar Asghar, Andrew Marshall, Mitra Tavakoli, Ahmed Al-Ahmar, Saad Javed, Maria Jeziorska, Rayaz A. Malik

“…The diagnostic performance of Neuropad was compared between the categorical and continuous (image-analysis (Sudometrics)) outputs to diagnose diabetic peripheral neuropathy (DPN). 110 subjects with type 1 and 2 diabetes underwent assessment with Neuropad, Neuropathy Disability Score (NDS), peroneal motor nerve conduction velocity (PMNCV), sural nerve action potential (SNAP), Deep Breathing-Heart Rate Variability (DB-HRV), intraepidermal nerve fibre density (IENFD), and corneal confocal microscopy (CCM). 46/110 patients had DPN according to the Toronto consensus. …”
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Biogenic silver nanoparticles produced by Trichoderma reesei inhibit SARS-CoV-2 infection, reduce lung viral load and ameliorate acute pulmonary inflammation by Marcus V.M.V. Amaral, Cláudia B. Carraro, Amanda C.C. Antoniêto, Mariana N. Costa, Thais F.C. Fraga-Silva, Ualter G. Cipriano, Rodrigo P.F. Abuná, Tamara S. Rodrigues, Ronaldo B. Martins, Andreia M. Luzenti, Glaucia R. Caruso, Priscyla D. Marcato, Vania L.D. Bonato, Dario S. Zamboni, Bergman M. Ribeiro, Sônia N. Báo, Joao S. da Silva, Flávio P. Veras, Roberto N. Silva

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Colon Epithelial MicroRNA Network in Fatty Liver by Paule V. Joseph, Sarah K. Abey, Dan Wang, Nicolaas H. Fourie, Natnael D. Kenea, Tatyana G. Vishnyakova, Jeffrey M. Robinson, Kristen R. Weaver, Christina M. Boulineaux, Hannah R. Davidson, LeeAnne B. Sherwin, Onyinyechi Ozoji, Ana F. Diallo, Paul A. Smyser, Amy P. Patterson, Wendy A. Henderson

“…Changes in barrier (transepithelial electrical resistance, TEER) and epithelial cell junction structure (Occludin and Zona Occludens-1/ZO-1 immunofluorescence staining-confocal microscopy) were examined pre- and posttransfection in Caco-2 cell monolayers. …”
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Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals by Ignacio Rafael Chiumiento, Ignacio Rafael Chiumiento, María Alejandra Tricerri, María Alejandra Tricerri, María Fernanda Cortéz, Santiago Ituarte, Santiago Ituarte, Julia Tau, Karina Valeria Mariño, Paola Lorena Smaldini, Horacio Heras, Horacio Heras, Marcos Sebastián Dreon, Marcos Sebastián Dreon

“…This species, known for its high reproductive rate and easy rearing, represents a promising source of potential biomedical compounds, including hemocyanin.MethodsEmploying flow cytometry, fluorescence microscopy, immunoassays, and quantitative PCR, we analysed the effects of PcH on THP-1 monocytes and their derived macrophages, as well as its ability to induce humoral response on C57BL/6 mice. …”
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Preparation of Composite Films Based on Methyl Cellulose/Polyvinyl Alcohol-loaded Nanosilver (AgNPs@PVA/MC) and Its Effect on Storage Quality of Ougan by Shuaishuai DAI, Weijie WU, Xiangjun FANG, Hangjun CHEN, Jun HUANG, Ruiling LIU, Huizhi CHEN, Ben NIU, Haiyan GAO

“…Among them, the composite film with 0.1% AgNPs content had the best comprehensive performance, its tensile strength reached 12.45 MPa, elongation at break was 6.47%, water vapor permeability was 21.03×10−6 g·mm/(m2·s·Pa), and oxygen permeability was 2.31×10−6 cm3/(cm2·s·Pa). Scanning electron microscopy (SEM) showed that composite film with 0.1% AgNPs content was regular, compact and evenly distributed in cross section. …”
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A key technology for synergistic backfilling of coal-based solid waste and high-salinity wastewater by Shuning DONG, Shujiang YU, Xingling DONG, Buqin ZHANG, Xiaoming GUO, Xiaodong WANG, Kai WANG, Shibin ZHU, Boqiang WU, Lei LIU

“…The mechanical properties, microstructures, and heavy metal leaching characteristics of the solidified filling paste were analyzed using the uniaxial compressive strength (UCS) test, scanning electron microscopy (SEM), and inductively coupled plasma-mass spectrometry (ICP-MS). …”
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Brucella suis ΔmapB outer membrane vesicles as an acellular vaccine against systemic and mucosal B. suis infection by Florencia Muñoz González, Florencia Muñoz González, Magali G. Bialer, Maria L. Cerutti, Silvia M. Estein, Lila Y. Ramis, Pablo C. Baldi, Pablo C. Baldi, Ángeles Zorreguieta, Ángeles Zorreguieta, Mariana C. Ferrero, Mariana C. Ferrero

“…In this study, we characterize the outer membrane vesicles (OMVs) of B. suis 1330 (wt) and those of B. suis ΔmapB (ΔmapB) mutant strain and evaluate their vaccine potential in mice.MethodsOMVs were isolated using the ultracentrifugation method and characterized through electron microscopy, Dynamic Light Scattering, SDS-PAGE and proteomics. …”
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Transient lymphocyte count decrease correlates with oncolytic adenovirus efficacy in humans: mechanistic and biomarker findings from TUNIMO phase I trial by Tuomo Alanko, Akseli Hemminki, Víctor Cervera-Carrascón, Riikka Havunen, Kristian Taipale, Dafne C A Quixabeira, Jorma Sormunen, Katriina Peltola, Suvi Sorsa, Santeri A Pakola, Elise Jirovec, Lyna Haybout, Claudia Kistler, Tatiana V Kudling, Victor Arias, James H A Clubb, Mirte van der Heijden, Teijo Pellinen, Joao M Santos

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Graphene oxide and silver effects on the arc erosion properties of Al2O3–Cu/30Cr composite by Qiujie Liu, Meng Zhou, Baohong Tian, Yi Zhang, Qimeng Zhang, Jiacan Zhang, Zhiyu Han, Chunhe Chu, Ke Jing, Xu Li, Huiwen Guo, Alex A. Volinsky

“…Furthermore, the transmission electron microscopy (TEM) and energy dispersive spectrometer (EDS) results show that Cr3C2 compounds effectively pin the Cr–Cu interface. …”
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Effect of Yanshiqiangxin Decoction on Cardiomyocyte Apoptosis in Heart Failure Rats Based on PI3K/Akt/Caspase-9 Signaling Pathway by ZHU Sixing, YAN Shiyun, XU Yan, QIN Yan, CHEN Liyun, JIA Meijun

“…One day after the last gavage, M-mode echocardiography [left ventricular ejection fraction (LVEF), left ventricular short-axis shortening (LVFS), left ventricular internal dimension diastole (LVIDd) and left ventricular internal dimension systole (LVIDs)] was performed through an ultra-high resolution small animal ultrasound imaging system; B-type natriuretic peptide (BNP) was measured by ELISA method; the apoptosis of cardiomyocytes was observed through laser confocal microscopy and the apoptosis index was calculated; the expression of apoptosis-related proteins Bax, Bcl-2 and PI3K/Akt/Caspase-9 signalling pathway proteins were detected by Western blot method.Results① Cardiac ultrasound test results: compared with the normal group, the left ventricular anterior wall motion waveform of the model group was not obvious, close to linear motion, and LVEF and LVFS decreased significantly (<italic>P</italic>&lt;0.05), LVIDd and LVIDs increased significantly (<italic>P</italic>&lt;0.05), the left ventricle was significantly enlarged and the contractility was reduced. …”
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Galactin-8 DNA methylation mediates macrophage autophagy through the MAPK/mTOR pathway to alleviate atherosclerosis by Bing Xia, Yan-lin Lu, Jin Peng, Jing-wei Liang, Fang-qin Li, Jiu-yang Ding, Chang-wu Wan, Cui-yun Le, Jia-lin Dai, Jie-Wang, Bing Guo, Jiang Huang

“…Detection of autophagosomes in macrophages by transmission electron microscopy was also performed. The foam cell model was induced with human monocytes (THP-1) and co-cultured with foam cells using siRNAs targeting GAL8, DO05, and UO126. …”
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Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies by Camilla T. Ekanger, Camilla T. Ekanger, Camilla T. Ekanger, Nilima Dinesh Kumar, Rosanne W. Koutstaal, Fan Zhou, Martin Beukema, Joanna Waldock, Simon P. Jochems, Noa Mulder, Cécile A. C. M. van Els, Cécile A. C. M. van Els, Othmar G. Engelhardt, Nathalie Mantel, Kevin P. Buno, Karl Albert Brokstad, Karl Albert Brokstad, Agnete S. T. Engelsen, Agnete S. T. Engelsen, Rebecca J. Cox, Rebecca J. Cox, Barbro N. Melgert, Barbro N. Melgert, Anke L. W. Huckriede, Puck B. van Kasteren

“…We first assessed the presence of various differentiated cell types using immunofluorescence microscopy. Using a shared stock of influenza A virus, we then assessed viral growth kinetics, epithelial cytokine responses, and serum-mediated inhibition of infection.ResultsThe presence of club, goblet, and ciliated cells was confirmed in all models. …”
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Lycium barbarum glycopeptide ameliorates aging phenotypes and enhances cardiac metabolism by activating the PINK1/Parkin-mediated mitophagy pathway in D-galactose-induced mice by Tianchan Peng, Jian Xiang, Yun Tian, Xiaogen Tang, Lina Wang, Lijuan Gao, Oscar Junhong Luo, Li’an Huang, Guobing Chen

“…Transmission electron microscopy was used to observe the ultrastructure of mitochondria. …”
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响应面优化氯化胆碱-丙三醇体系提取芝麻蛋白工艺条件 "Response surface optimization of the extraction process conditions for sesame protein using choline chloride-glycerol system"... by 曹培换1，张晨霞1，刘燕2，马宇翔1，刘华敏1，郑永战1，3 "CAO Peihuan1，ZHANG Chenxia1，LIU Yan2，MA Yuxiang1， LIU Huamin1，ZHENG Yongzhan1,3"

“…Fourier transform infrared spectroscopy and scanning electron microscopy were used to characterize the structure of sesame protein. …”
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Antiedemic Effect of the Myosin Light Chain Kinase Inhibitor PIK7 in the Rat Model of Myocardial Ischemia Reperfusion Injury by Dmitry L. Sonin, Mikhail S. Medved, Asker Y. Khapchaev, Maria V. Sidorova, Marina E. Palkeeva, Olga A. Kazakova, Garry V. Papayan, Daniil A. Mochalov, Sarkis M. Minasyan, Ilya E. Anufriev, Daria V. Mukhametdinova, Natalia M. Paramonova, Ksenia M. Balabanova, Anastasia S. Lopatina, Ilia V. Aleksandrov, Natalya Yu. Semenova, Anna A. Kordyukova, Kirill V. Zaichenko, Vladimir P. Shirinsky, Michael M. Galagudza

“…The early no-reflow detected for the first time after 30 min ischemia in this model of myocardial infarction reduces the area accessible for PIK7. Electron microscopy has shown membrane-bound blebs of endotheliocytes, which partially or completely obturate the capillary lumen, and few capillaries with signs of intercellular gap formation in samples obtained from the center of the early no-reflow zone in control and PIK7-injected rats. …”
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Enhancement of physicochemical and techno-functional properties of soy protein isolate amyloid fibrils by moderate ultrasonic pretreatment by Yunqi Ni, Tianyi Yan, Kai Fu, Congyi Xu, Liang Zhang, Donghong Liu, Wenjun Wang

“…Morphological examination by transmission electron microscopy exhibited a higher maturation state, showing more rigid and straight fibrils structure in SPF10 compared to SPF0. …”
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Proteasome inhibition enhances oncolytic reovirus therapy in multiple myeloma independently of its direct cytotoxic effects by Ada Alice Dona, Theophilus Tandoh, Lokesh Nigam, Mahmoud Singer, Enrico Caserta, Mariam Murtadha, Yinghui Zhu, Milad Moloudizargari, Preeti Sharma, Ottavio Napolitano, Janet Winchester, Arnab Chowdhury, Alex Pozhitkov, James F. Sanchez, Hawa Vahed, Guido Marcucci, Matt Coffey, Gerard Nuovo, Douglas W. Sborov, Flavia Pichiorri, Craig C Hofmeister

“…The combination with proteasome inhibitors (PIs) has shown to enhance RV therapeutic activity, but the mechanisms of action have not been fully elucidated. Methods Electron microscopy, q-RT-PCR, single-cell mass cytometry (CyTOF), flow cytometry, plaque assays, immunohistochemistry, and Western blot analysis were used to assess RV infection of both myeloma and immune cells. …”
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