Comprehensive Structural, Chemical, and Optical Characterization of Cu₂ZnSnS₄ Films on Kapton Using the Automated Successive Ionic Layer Adsorption and Reacti... by Perla J. Vázquez-González, Martha L. Paniagua-Chávez, Lizette A. Zebadua-Chavarria, Rafael Mota-Grajales, C. A. Meza-Avendaño, Enrique Campos-González, A. Escobosa-Echavarría, Yaoqiao Hu, Aldo E. Pérez-Ramos, Manuel-Matuz, Carlos A. Hernández-Gutiérrez

“…Field emission scanning electron microscopy (FE-SEM) showed enhancements in surface morphology, with increased grain size and uniformity post-annealing. …”
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Pink1/Parkin signaling mediates pineal mitochondrial autophagy dysfunction and its biological role in a comorbid rat model of depression and insomnia by Zirong Li, Yi Shu, Deguo Liu, Sheng Xie, Liangbo Xian, Jiaqi Luo, Xiuwen Huang, Haixing Jiang

“…Transmission electron microscopy of the pineal gland in CUMS+SD rats revealed damage to mitochondrial autophagy. …”
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In-vitro and in-vivo assessment of biocompatibility and efficacy of ostrich eggshell membrane combined with platelet-rich plasma in Achilles tendon regeneration by Omid Koohi-Hosseinabadi, Reza Shahriarirad, Amireza Dehghanian, Laleh Amini, Sajjad Barzegar, Afrooz Daneshparvar, Omid Alavi, Seyedeh-Parvin Khazraei, Saba Hosseini, Ali Arabi Monfared, Roya Khorram, Nader Tanideh, Soheil Ashkani-Esfahani

“…High-resolution scanning electron microscopy (HRSEM) was utilized to visualize the sample morphology and fiber bonding. …”
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Influence of substrate bias on the properties of CrAlN wear-resistant coating prepared by arc/magnetron composite technology by Xiaoyan NIU, Mingze LIU, Guoqiang DONG, Yujiang WANG, Haoliang TIAN

“…The research comprehensively analyzes how varying substrate bias voltage influences the microstructural morphology, mechanical properties, and frictional behavior of the coatings using scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction, nanoindentation, scratch test, and friction and wear tests. …”
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Leveraging AI to automate detection and quantification of extrachromosomal DNA to decode drug responses by Kohen Goble, Aarav Mehta, Damien Guilbaud, Jacob Fessler, Jingting Chen, William Nenad, William Nenad, Christina G. Ford, Oliver Cope, Darby Cheng, William Dennis, Nithya Gurumurthy, Yue Wang, Kriti Shukla, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk

“…Extrachromosomal DNA (ecDNA), in contrast, represents a rapid, reversible, and predictable DNA alteration critical for cancer’s adaptive response.MethodsIn this study, we developed a novel post-processing pipeline for automated detection and quantification of ecDNA in metaphase Fluorescence in situ Hybridization (FISH) images, leveraging the Microscopy Image Analyzer (MIA) tool. This pipeline is tailored to monitor ecDNA dynamics during drug treatment.ResultsOur approach effectively quantified ecDNA changes, providing a robust framework for analyzing the adaptive responses of cancer cells under therapeutic pressure.DiscussionThe pipeline not only serves as a valuable resource for automating ecDNA detection in metaphase FISH images but also highlights the role of ecDNA in facilitating swift and reversible adaptation to epigenetic remodeling agents such as JQ1.…”
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CPT1 deficiency blocks autophagic flux to promote lipid accumulation induced by co-exposure to polystyrene microplastic and cadmium by Zhixuan Chen, Huayi Qu, Jian Sun, Tao Wang, Yan Yuan, Yan Yuan, Yan Yuan, Jianhong Gu, Jianhong Gu, Jianhong Gu, Jianchun Bian, Jianchun Bian, Jianchun Bian, Zongping Liu, Zongping Liu, Zongping Liu, Hui Zou, Hui Zou, Hui Zou

“…As observed by transmission electron microscopy (TEM), the number of autophagosomes was significantly increased in the PS-MPs and Cd co-treated group. …”
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Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…The characterization of the CG was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Raman spectroscopy, thermogravimetric analysis (TGA), and X-ray diffraction (XRD). …”
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Evaluating oxide nanoparticle exsolution on A-site deficient PrBaCo2O6-δ electrodes by Alfonso J Carrillo, María Balaguer, Cecilia Solís, Andrés López-García, Sylvio Haas, María Fabuel, Blanca Delgado-Galicia, Isabelle Rodriguez, Einar Vøllestad, Sebastian Wachowski, Ragnar Strandbakke, Truls Norby, Jose M Serra

“…Combined Raman spectroscopy and electron microscopy analysis revealed that BaCoO _3 , Co _3 O _4 , and amorphous BaCO _3 nanoparticles were formed on the surface of the double perovskites after the reductive treatments. …”
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Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38 by Knut Husø Lauritzen, Knut Husø Lauritzen, Kuan Yang, Michael Frisk, Mieke C. Louwe, Maria Belland Olsen, Maria Belland Olsen, Mathias Ziegler, William E. Louch, Bente Halvorsen, Bente Halvorsen, Pål Aukrust, Pål Aukrust, Arne Yndestad, Øystein Sandanger, Øystein Sandanger

“…Ca2+ mobilization was determined by live confocal microscopy. NLRP3 activity was also compared in WT and CD38-/- mouse bone marrow-derived macrophages (BMDMs) with and without CD38 inhibitors.ResultsApigenin significantly inhibited IL-1β release from LPS-primed monocytes and macrophages activated with ATP, MSU, or nigericin. …”
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Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration by You Wu, Xiaokun Yue, Ying Zhang, Ning Yu, Chengyan Ge, Rui Liu, Zhongying Duan, Lilong Gao, Xinlong Zang, Xin Sun, Deteng Zhang

“…Functional group analysis, surface morphology and hydrophilicity were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and an optical contact angle measuring instrument, respectively. …”
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Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0... by Alena S. Ovchinnikova, Dmitrii A. Odnoshevsky, Alexey S. Kabanov, Sergey A. Bodnev, Oleg V. Pyankov, Oksana P. Os'kina, Maria V. Sivay, Andrey V. Bespalov, Tatyana V. Tregubchak, Larisa N. Shishkina, Oleg S. Taranov, Vladimir V. Zolin, Artemiy A. Sergeev, Alexander P. Agafonov

“…In this study, the pathogen of monkeypox was detected and identified using real-time PCR, NGS and electron microscopy, and the first imported case of this disease in Russia was confirmed. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…Biofilm viability was assessed using CAM/TMA-DPH or SYTO9/PIstaining, followed by analysis with confocal laser scanning microscopy (CLSM) and ImageJ-based biofilm surface coverage quantification. …”
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Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 by Shen Chen, Kexin Nie, Hongzhan Wang, Yang Gao, Xinyue Jiang, Hao Su, Zhi Wang, Yueheng Tang, Fuer Lu, Hui Dong, Jingbin Li

“…Brown adipose tissue (BAT) activity was evaluated using Positron Emission Tomography, and ultrastructural changes were examined via transmission electron microscopy. Proteomic analysis identified targets of WMW in obesity treatment. …”
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Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut m... by Lei Li, Lei Li, Lei Li, Jun Ma, Jun Ma, Zeshi Li, Zeshi Li, Juelin Chen, Jiawei Zhou, Yawei Wang, Yankun Pei, Yitong Gong, Jianyao You, Yangyang Cao, Man Wang, Jikuai Chen, Wenjun Chang, Weiyi Ma, Hanyu Zhu, Chuhan Xiang, Shuogui Xu, Qing Song, Qing Song

“…Transmission electron microscopy confirmed the preservation of tight junction structures. …”
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plaTform fOr Urinary tract infection diagnostiC evAluatioN (TOUCAN): a protocol for a prospective diagnostic accuracy study of point-of-care testing in patients suspected of acute... by Thomas R Fanshawe, Philip J Turner, Gail N Hayward, Margaret Glogowska, Alastair D Hay, Michael Moore, Mark Lown, Christopher C Butler, Mandy Wootton, Mark H Wilcox, Jane Freeman, Nicola Kenealy, Valerie Tate, Owain Llion, Rebecca Lowe

“…The second part of the sample will be processed at a National Health Service-based reference laboratory using a modified reference standard including microscopy, microbiological culture, pathogen speciation and antimicrobial susceptibility testing. …”
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Technological dental sealants: in vitro evaluation of material properties and antibiofilm potential by Witalo Pereira de Jesus, Luiza de Almeida Queiroz Ferreira, Washington Henrique Themoteo da Silva, Fernanda Vieira Belém, Ana Paula Turrioni, Cláudia Silami de Magalhães, Mila Fernandes Moreira Madeira, Ivana Márcia Alves Diniz, Marco Aurelio Benini Paschoal

“…Abstract Background Due to the launch of new self-etching and self-adhesive sealing materials combined with the release of bioactive ions in the dental market, this in vitro study aimed to compare physical values of surface roughness (RS), vickers microhardness (VM), compositional energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), microbiological tests (inhibition halo-HI and surface microbial adhesion-MAS) and mechanical microshear bond strength (MBS). …”
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Enhancing thermoelectric properties of ScN films through twin domains by J. More-Chevalier, U.D. Wdowik, J. Martan, T. Baba, S. Cichoň, P. Levinský, D. Legut, E. de Prado, P. Hruška, J. Pokorný, J. Bulíř, C. Beltrami, T. Mori, M. Novotný, I. Gregora, L. Fekete, L. Volfová, J. Lančok

“…The microstructure of the produced thin films is examined by X-ray diffraction and atomic force microscopy, while their chemical composition and contamination by defects are determined by X-ray photoelectron spectroscopy. …”
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Green biosynthetic silver nanoparticles from Ageratum conyzoides as multifunctional hemostatic agents: Combining hemostasis, antibacterial, and anti-inflammatory properties for eff... by Yang Li, Yinfeng Tan, Huange Zhao, Shiting Chen, Azadeh Nilghaz, Rong Cao, Songlin Zhou

“…Subsequent experiments including scanning electron microscopy, flow cytometry, western blotting, and network pharmacology analysis have revealed that AC-AgNPs can cause platelet activation and aggregation, stimulated kallikrein-kinin system, shorten activated partial thromboplastin and prothrombin time, and increase fibrinogen content. …”
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Therapeutic effect of dihydroartemisinin on Alzheimer’s disease model mice with senile macular degeneration by Gao Han, Gong Xuewu, Zhang Meng, Wang Yuejing, Wang Yuchun, Zhang Keshuang, Yao Hongbo

“…Transmission electron microscopy showed that DHA and donepezil could reduce mitochondrial vacuolation and synaptic edema. …”
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Structural basis of FpGalNase and its combination with FpGalNAcDeAc for efficient A-to-O blood group conversion by Meiling Zhou, Kaishan Luo, Chao Su, Yan Sun, Zuyan Huang, Shuo Ma, Xun Gao, Jiwei Wang, Chen Zhang, Pengcheng Han, Guoqiu Wu

“…Using cryo-electron microscopy, we resolved the full-length structure of FpGalNase, identifying critical active site residues involved in its catalytic mechanism. …”
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