Deep eutectic solvent enhances antibacterial activity of a modular lytic enzyme against Acinetobacter baumannii by Aleksandra Maria Kocot, Tomasz Swebocki, Karolina Ciemińska, Adrianna Łupkowska, Małgorzata Kapusta, Dennis Grimon, Ewa Laskowska, Anna-Karina Kaczorowska, Tadeusz Kaczorowski, Rabah Boukherroub, Yves Briers, Magdalena Plotka

“…Confocal fluorescence microscopy indicated that reline damaged the structure of the biofilm, allowing MLE-15 to penetrate it. …”
Get full text

Microbial Surface Confined Growth Strategy for the Synthesis of Highly Loaded NiCoP Nanoparticles with Hollow Derived Carbon Shells for Sodium Ion Capture by Jianhua Yuan, Tianxiao Sun, Jinfeng Chen, Runhong Zhou, Jianglin Cao, Fei Yu, Liqing Li, Xiumin Zhong, Jie Ma

“…Scanning transmission X‐ray microscopy (STXM) analysis confirmed the reversible conversion reaction mechanism and the robust structure of NiCoP@NPC before and after the reaction; Density function theory (DFT) and electrochemical quartz crystal microbalance (EQCM‐D) further confirmed that the structural design of NiCoP@NPC promoted electron transport, Na+ adsorption as well as improved cycling stability. …”
Get full text

Enhanced radiation shielding via incorporating europium oxide in 316L stainless steel: Synthesis, physical, microstructural, shielding, and mechanical properties by H.O. Tekin, Nihal Yayla, M.Gökhan Albayrak, Ömer Güler, Duygu Sen Baykal, Hessa Alkarrani, Ghada ALMisned

“…X-ray diffraction (XRD) analysis revealed that the face-centered cubic (FCC) structure of the steel matrix was preserved up to 5% Eu2O3 reinforcement, while higher concentrations led to phase formation and crystallographic changes. Scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis showed uniform element distribution at low reinforcement levels, with particle clustering at 20% Eu2O3. …”
Get full text

Oil palm empty fruit bunch biochar fertilizer as a solution to increasing the fertility of peat soil for sustainable agriculture by Sri Nuryani Hidayah Utami, Uray Suci Yulies Vitri Indrawati

“…Then the biochar is weighed to get the desired proportion of biochar then sieved through a 60 mesh sieve.nd analyzed for pH, ash content, organic-C, total-N, total-P, total-K, as well as biochar functional groups using Fourier Transform Infrared Spectroscopy (FTIR), and structural analysis using Scanning Electron Microscopy (SEM). The implementation of the research consisted of 2 activities, namely 1) Planting oil palm seedlings in polybags by giving treatment according to the dose and 2) Chemical characterization of biochar, peat soil properties and growth and NPK uptake of oil palm seedlings. …”
Get full text

Combined multi-scale characterization of pores in ultra-thick coal seams of Jurassic Xishanyao Formation, Tiaohu-Malang sags, Santanghu Basin by Yue CHEN, Qiqi LEI, Dongmin MA, Xin WANG, Xinggang WANG, Diefang HUANG, Gaoxiang RONG

“…Techniques such as high-pressure mercury intrusion porosimetry, low-temperature liquid nitrogen adsorption, nuclear magnetic resonance (NMR), CT scanning, scanning electron microscopy (SEM), and the pore-crack analysis system (PCAS) were used to investigate pore development characteristics. …”
Get full text

<i>Bacillus thuringiensis</i> Cry1A Insecticidal Toxins and Their Digests Do Not Stimulate Histamine Release from Cultured Rat Mast Cells by Hisashi Ohto, Mayumi Ohno, Miho Suganuma-Katagiri, Takashi Hara, Yoko Egawa, Kazuya Tomimoto, Kosuke Haginoya, Hidetaka Hori, Yuzuri Iwamoto, Tohru Hayakawa

“…However, fluorescence microscopy revealed no specific binding of Cry1A toxins to RBL-2H3 cultured rat mast cells. …”
Get full text

Effects of ZnO nanoparticles concentration on the morphology and textural properties of ZnO/NiFe2O4 nanocomposite by Jimoh Oladejo Tijani, Augustine Innalegwu Daniel, Sarah Udenyi Onogwu, Ambali Saka Abdulkareem, Usman Ahmed Aminu, Marshall Keyster, Ashwil Klein

“…High resolution transmission electron microscopy (HRTEM) images showed that the average interplanar distance of wurtzite phase at 3, 5, and 7 % dopant concentration were about 0.28, 0.44 and 0.33 nm respectively. …”
Get full text

Spatial Microstructural Ordering of Expansive Clay Minerals by T. López-Lara, J. B. Hernández-Zaragoza, D. Carreón-Freyre, M. Cerca, E. Rojas-González, A. G. Minor Franco, G. Martínez-Barrera, R. Salgado-Delgado

“…The clay microstructure was obtained using X-ray diffraction, and sweeping electronic microscopy. Physical characterization includes pH analysis. …”
Get full text

Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α by Runjie Guo, Ping Chen, Tiantian Fu, Ren Zhang, Yuan Zhu, Nange Jin, Hong Xu, Yong Xia, Xuesong Tian

“…Morphological changes were observed by inverted microscopy. TNF-α protein levels in cell lysates were measured by enzyme-linked immunosorbent assay (ELISA). …”
Get full text

Enhancement of host defense against Helicobacter pylori infection through modulation of the gastrointestinal microenvironment by Lactiplantibacillus plantarum Lp05 by Yao Dong, Mei Han, Yongmei Qi, Ying Wu, Zhipeng Zhou, Dacheng Jiang, Zhonghui Gai

“…ObjectiveThis study aimed to assess the impact of Lactiplantibacillus plantarum Lp05 (Lp05) on the gastrointestinal microbiome and pathophysiological status of mice infected with Helicobacter pylori (H. pylori), exploring its potential as a probiotic treatment for H. pylori infections.MethodsIn vitro, the interaction between Lp05 and H. pylori was analyzed using laser confocal and scanning electron microscopy. In vivo, C57BL/6 mice infected with H. pylori were treated with Lp05 and divided into six groups: control, model, quadruple therapy, and three dosage levels of Lp05 (2×107, 2×108, 2×109 CFU/mouse/day). …”
Get full text

Exosomes and their distinct integrins transfer the characteristics of oxaliplatin- and 5-FU-resistant behaviors in colorectal cancer cells by Zeynab Vakili-Ghartavol, Hoda Deli, Amir Shadboorestan, Roxana Sahebnasagh, Elahe Motevaseli, Mohammad Hossein Ghahremani

“…Exosomes were isolated via ultracentrifugation and characterized using DLS and electron microscopy to evaluate size and morphology. Western blot analysis was employed to identify exosomal markers. …”
Get full text

Transport and inhibition of the sphingosine-1-phosphate exporter SPNS2 by Huanyu Z. Li, Ashley C. W. Pike, Yung-Ning Chang, Dheeraj Prakaash, Zuzana Gelova, Josefina Stanka, Christophe Moreau, Hannah C. Scott, Frank Wunder, Gernot Wolf, Andreea Scacioc, Gavin McKinley, Helena Batoulis, Shubhashish Mukhopadhyay, Andrea Garofoli, Adán Pinto-Fernández, Benedikt M. Kessler, Nicola A. Burgess-Brown, Saša Štefanić, Tabea Wiedmer, Katharina L. Dürr, Vera Puetter, Alexander Ehrmann, Syma Khalid, Alvaro Ingles-Prieto, Giulio Superti-Furga, David B. Sauer

“…Here, we use a combination of cryo-electron microscopy, immunofluorescence, in vitro binding and in vivo S1P export assays, and molecular dynamics simulations to probe SPNS2’s substrate binding and transport. …”
Get full text

Tuning CO2 reduction selectivity via structural doping of TiO2 photocatalysts by Hana Kmentová, Miroslava Filip Edelmannová, Zdeněk Baďura, Radek Zbořil, Lucie Obalová, Štěpán Kment, Kamila Kočí

“…High-resolution transmission electron microscopy revealed uniformly distributed active sites on platinum-doped TiO₂ surfaces. …”
Get full text

Co-obligate symbioses have repeatedly evolved across aphids, but partner identity and nutritional contributions vary across lineages by Manzano-Marín, Alejandro, Coeur d’acier, Armelle, Clamens, Anne-Laure, Cruaud, Corinne, Barbe, Valérie, Jousselin, Emmanuelle

“…Fluorescent in situ hybridisation microscopy shows a common bacteriocyte localisation of two newly acquired symbionts. …”
Get full text

Cell Morphology on Poly(methyl methacrylate) Microstructures as Function of Surface Energy by Matthias Katschnig, Boris Maroh, Natascha Andraschek, Sandra Schlögl, Ulrike Zefferer, Elisabeth Bock, Gerd Leitinger, Christa Trattnig, Maria Kaufmann, Werner Balika, Clemens Holzer, Ute Schäfer, Silke Patz

“…The surface energy of the resultant microstructures was determined by static contact angle measurements. Light scanning microscopy of the morphology of NT2/D1 and MC3T3-E1 preosteoblast cells cultured on structured PMMA samples in both cases revealed a profound surface energy dependence. …”
Get full text

Nuclear talin-1 provides a bridge between cell adhesion and gene expression by Alejandro J. Da Silva, Hendrik S.E. Hästbacka, Mikael C. Puustinen, Jenny C. Pessa, Jens C. Luoto, Erika Sundström, Benjamin T. Goult, Guillaume Jacquemet, Eva Henriksson, Lea Sistonen

“…By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. …”
Get full text

Citronellol-functionalized natural silica: a biogenic approach for antifungal and antibacterial material applications by Guillermo P. Lopez, Guillermo P. Lopez, Leyanet Barberia Roque, Katerine Igal, Katerine Igal, Erasmo Gámez Espinosa, Erasmo Gámez Espinosa, Natalia Bellotti, Natalia Bellotti

“…The hybrids obtained were characterized by thermogravimetric analysis (TGA), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier-transform infrared spectroscopy (FTIR). …”
Get full text

Selective Serotonin Reuptake Inhibitors: Antimicrobial Activity Against ESKAPEE Bacteria and Mechanisms of Action by Thiago Hideo Endo, Mariana Homem de Mello Santos, Sara Scandorieiro, Bruna Carolina Gonçalves, Eliana Carolina Vespero, Márcia Regina Eches Perugini, Wander Rogério Pavanelli, Gerson Nakazato, Renata Katsuko Takayama Kobayashi

“…Both drugs effectively eradicated bacterial populations within 2 to 24 h and caused morphological changes, such as protrusions and cellular fragmentation, as shown by electron scanning microscopy. Regarding their mechanisms of action as antibacterials, for the first time, increased membrane permeability was detected, as evidenced by heightened dye absorption, along with the increased presence of total proteins and dsDNA in the extracellular medium of <i>Escherichia coli</i> ATCC2 25922 and <i>Staphylococcus aureus</i> ATCC 25923, and oxidative stress was also detected in bacteria treated with sertraline and paroxetine, with reduced efficiency observed in the presence of antioxidants and higher levels of oxygen-reactive species evidenced by their reaction with 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate. …”
Get full text

Design, defect analysis, compressive strength and surface texture characterization of Laser Powder Bed Fusion processed Ti6Al4V lattice structures by Bruno Zluhan, Shubhavardhan Ramadurga Narasimharaju, Abhijit Cholkar, Ken Thomas, Ramesh Raghavendra, Eder S.N. Lopes

“…The surface roughness, defects and porosities are analysed using Alicona, optical microscopy, SEM and XCT techniques. Pycnometer and compressive testing are adopted to study apparent density and ultimate compressive strength. …”
Get full text

Click chemistry-enabled gold nanorods for sensitive detection and viability evaluation of copper(II)-reducing bacteria by Tongtong Tian, Wenjing Yang, Xiaohuan Wang, Te Liu, Baishen Pan, Wei Guo, Beili Wang

“…Our method, termed AuNRs–bacteria-initiated click chemistry (AuNRs–BICC), detects CuII-reducing bacteria by quantifying AuNRs bound to a biosensing interface via bacteria-mediated CuII reduction to CuI and subsequent click chemistry with biosensing interface of azide modifications. Using dark-field microscopy (DFM), we demonstrated a strong linear correlation between AuNR counts and the logarithm of bacterial concentration for both Gram-negative Escherichia coli (including KPC-2-expressing antibiotic-resistant strains) and Gram-positive Staphylococcus aureus across a range of 101 to 107 cells, achieving a remarkable detection limit of 101 cells. …”
Get full text