Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology by Mattia Santoni, Giovanni Piccinini, Giovanni Piccinini, Giovanni Liguori, Maria Roberta Randi, Massimo Baroncini, Liliana Milani, Francesca Danesi

“…Here, we developed an enhanced Caco-2/HT29-MTX-E12 co-culture model incorporating orbital mechanical stimulation to better replicate intestinal conditions and investigate nanoparticle interactions.MethodsWe established co-cultures under static and dynamic conditions, evaluating their development through multiple approaches including barrier integrity measurements, gene expression analysis, and confocal microscopy. We introduced novel quantitative analysis of dome formation as a differentiation marker and demonstrated the model application by investigating cellular responses to titanium dioxide (TiO₂) nanoparticles in a digested food matrix.ResultsDynamic conditions accelerated epithelial differentiation, achieving functional barrier properties by day 14 rather than day 21, with enhanced mucin production and more organized three-dimensional structure. …”
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Age and diet affect self-resilience of intestinal microbiome in mice by Chenyi Shao, Shenmin Chen, Huan Yang, Mufan Li, Yinhui Liu, Shu Wen, Jing Xiao, Li Tang

“…This study aimed to investigate the self-resilience of the gut microbiome at different ages and the effects of diet on its recovery capacity in adulthood.MethodsA rodent model of antibiotic-induced dysbiosis was used. Microscopy was used to observe morphological changes in the mucosa. …”
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Saponins Effect on Human Insulin Amyloid Aggregation by Eleonora Mari, Silvia Vilasi, Paolo Moretti, Maria Rosalia Mangione, Giorgia Giorgini, Roberta Galeazzi, Maria Grazia Ortore

“…The morphology of the aggregates was assessed by Atomic Force Microscopy. To deepen the understanding of the saponins interaction with insulin, a Molecular Dynamics investigation is performed, too. …”
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Formulation, Characterization, Antibacterial Activity, Antioxidant Activity, and Safety Evaluation of <i>Camphora longepaniculata</i> Essential Oil Nanoemulsions Through High-Press... by Yue Yan, Changhe Wei, Xin Liu, Xin Zhao, Shanmei Zhao, Shuai Tong, Guoyou Ren, Qin Wei

“…The formation of nanoemulsions was verified by Fourier transform infrared spectroscopy and transmission electron microscopy. Homogenized nanoemulsions had smaller particle sizes of 39.99 ± 0.47 nm and exhibited enhanced stability. …”
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Effects of sodium tripolyphosphate on the rheological properties of concrete: Mechanical, microstructural and carbon footprint by André Pico-Molineros, Natividad García-Troncoso, Jorge Flores-Rada, Dan V. Bompa

“…Testing was conducted in accordance with international and national standards, including slump, compressive strength, air content, setting time, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and embodied carbon assessment. …”
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Enhanced Photocatalytic Degradation of Methylene Blue Dye Using Co3O4 Nanoparticles from the Fruit Extracts of Diplocyclos palmatus (L) C. Jeffrey for Wastewater Remediation by Parethe Gurusamy Thangam, Rajesh Pattulingam, Velmani Vellaiyan, Balaji Murugaesan, Kavica Sathasivam

“…The nanoparticles were characterized for UV- visible light spectra, XRD analysis, Scanning electron microscopy (SEM) and energy - dispersive X-ray (EDX) spectroscopy showing their irregular, small particles with stakes of various sizes. …”
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Mesoscopic fracture damage evolution and fractal damage constitutive model of heat-treated red sandstone under direct tensile impact loadings by Shi Liu, Yu Jia, Yue Zhai, Shaoxu Hao

“…An improved scanning electron microscopy (SEM) experimental method was used to analyze the tensile fracture surfaces of rock samples. …”
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Decision tree-based learning and laboratory data mining: an efficient approach to amebiasis testing by Enas Al-khlifeh, Ahmad S. Tarawneh, Khalid Almohammadi, Malek Alrashidi, Ramadan Hassanat, Ahmad B. Hassanat

“…The primary diagnostic method in laboratories involves the microscopy of stool samples. However, this approach can sometimes result in the misinterpretation of amebiasis as other gastroenteritis (GE) conditions. …”
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Kaposi's sarcoma-associated herpesvirus (KSHV) gB dictates a low-pH endocytotic entry pathway as revealed by a dual-fluorescent virus system and a rhesus monkey rhadinovirus expres... by Shanchuan Liu, Sarah Schlagowski, Anna K Großkopf, Natalia Khizanishvili, Xiaoliang Yang, Scott W Wong, Elina M Guzmán, Marija Backovic, Stefano Scribano, Arne Cordsmeier, Armin Ensser, Alexander S Hahn

“…We demonstrated lytic reactivation and infectivity of dual-fluorescent KSHV. Confocal microscopy was used to quantify co-localization of fluorescently-tagged glycoproteins and capsid proteins. …”
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Evaluation of Antimicrobial and Wound Healing Effects of Gold Nanoparticles Containing Abelmoschus esculentus (L.) Aqueous Extract by Shahla Korani, Khodabakhsh Rashidi, Mona Hamelian, Ali R. Jalalvand, Ahmad Tajehmiri, Mitra Korani, Thozhukat Sathyapalan, Amirhossein Sahebkar

“…The Ok Au NPs were characterized using various techniques like UV-Vis absorption spectroscopy, FTIR, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Cutaneous wounds were created on 30 rats and randomized into three groups: untreated and two groups treated with Ch Au NPs and Ok Au NPs. …”
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Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples by Ibrahim N. Mwangi, Eric L. Agola, Robert M. Mugambi, Esther A. Shiraho, Gerald M. Mkoji

“…The LAMP assay was evaluated against the microscopy-based procedure and the results were analysed using Cohen’s kappa coefficient to determine the degree of agreement between the two techniques. …”
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Smart Zinc-Based Coatings with Chitosan–Alginate Nanocontainers Loaded with ZnO and Caffeine for Corrosion Protection of Mild Steel by Kamelia Kamburova, Nelly Boshkova, Tsetska Radeva, Nikolai Boshkov

“…The surface morphology and elemental composition of the electrodeposited hybrid coatings before and after treating in the model corrosive medium of 3.5% NaCl is studied by scanning electron microscopy (SEM). The cyclic voltammetry method (CVA) is applied to characterize the cathodic (electrodeposition) and anodic (dissolution) processes. …”
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Study of Structural, Dielectric, and Phase Transition of Strontium Doped BaTiO₃ by Asmaa Zouitine, Abdelhak El Ghandouri, Abdelhalim Elbasset, Farid Abdi, Taj-dine Lamcharfi, Hicham Laribou, Lamiae Mrharrab, Zouhairi Mohammed

“…The results obtained from the X-ray diffraction spectrum confirmed the creation of the pure perovskite structure, with the diffraction peaks of the BSxT shifting toward the bigger 2θ with increasing Sr content. Scanning electron microscopy images show that the grain size of the BSxT ceramics decreases with increasing Sr content. …”
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Process Mineralogy and Occurrence State of Lithium of Spodumene Tailings in Sichuan Province by Panpan WANG, Junfeng WU, Lin CHEN, Yanqing CHEN

“…The samples were identified by optical microscopy, artificial heavy sand research (separation, identification, etc.), optical semi-quantitative analysis, chemical analysis, MLA automatic quantitative detection of minerals, LA-ICPMS in-situ analysis of mineral trace elements, and electron microscope energy spectrum analysis, X-ray powder crystal diffraction analysis and other work. …”
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Selective Solid-Phase Extraction of Pb(II) Ions Using an Ion-Imprinted Functionalized Sodium Trititanate Whisker Adsorbent Prepared by a Surface-Imprinting Technique by Wanzhen Xu, Wenming Yang, Wei Zhou

“…The prepared sorbent was characterized by Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). A series of batch adsorption experiments was performed to evaluate the adsorption behaviour of the Pb(II)-IIPs/Na 2 Ti 3 O 7 materials towards Pb(II) ions, as assessed by inductively coupled plasma–atomic emission spectrometry (ICP–AES). …”
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Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells by Juo Lee, Sungmin Lee, Iksong Byun, Myung Chul Lee, Jungsil Kim, Hoon Seonwoo

“…The morphology and orientation of the fibers were confirmed through electron microscopy. X-ray diffraction spectrometry was employed to confirm the integration of RGO and PCL. …”
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High-entropy assisted capacitive energy storage in relaxor ferroelectrics by chemical short-range order by Tongxin Wei, Jinzhu Zou, Xuefan Zhou, Miao Song, Yan Zhang, Cewen Nan, Yuanhua Lin, Dou Zhang

“…Atomic-scale scanning transmission electron microscopy observations show that Nb exhibits a chemical short-range order structure, with Nb enriched regions displaying ultrasmall polar nanoregions and more flexible polarization configurations, which is conducive to achieving high maximum polarization and low residual polarization. …”
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Development and performance evaluation of sustainable lightweight geopolymer based fireproofing coatings for steel construction by Zhenyu Huang, Yingwu Zhou, Hammad Salahuddin

“…X-ray diffraction and scanning electron microscopy were carried out to examine the micro-morphology of the samples, revealing that the main component of the geopolymer was Ca2(Al2SiO7) in a colloidal state at 30℃, 300℃, and 600℃. …”
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Structural insights into light-gating of potassium-selective channelrhodopsin by Takefumi Morizumi, Kyumhyuk Kim, Hai Li, Probal Nag, Tal Dogon, Oleg A. Sineshchekov, Yumei Wang, Leonid S. Brown, Songhwan Hwang, Han Sun, Ana-Nicoleta Bondar, Igor Schapiro, Elena G. Govorunova, John L. Spudich, Oliver P. Ernst

“…Here, we use single-particle cryo-electron microscopy of peptidisc-incorporated protein samples to determine the structures of the slow-cycling mutant C110A of kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) in the dark and upon laser flash excitation. …”
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Chronic Unexpected Mild Stress Destroys Synaptic Plasticity of Neurons through a Glutamate Transporter, GLT-1, of Astrocytes in the Ischemic Stroke Rat by Dafan Yu, Zhenxing Cheng, Abdoulaye Idriss Ali, Jiamin Wang, Kai Le, Enkhmurun Chibaatar, Yijing Guo

“…We exposed the ischemic stroke rats to ceftriaxone, during the CUMS intervention period to determine the effects of GLT-1 on glutamate circulation by immunofluorescence and mass spectrometry and its influences to synaptic plasticity by western blot and transmission electron microscopy. Result. CUMS evidently reduced the level of astroglial GLT-1 in the hippocampus of the ischemic rats (p<0.05), resulting in smaller amount of glutamate being transported into astrocytes surrounding synapses (p<0.05), and then expression of synaptophysin was suppressed (p<0.05) in hippocampal dentate gyrus. …”
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