Targeting LINC02320 prevents colorectal cancer growth via GRB7-dependent inhibition of MAPK signaling pathway by Lingwei Zhang, Hong Chen, Yangmei Yang, Liangbo Zhao, Huimin Xie, Peixian Li, Xinrui Lv, Luyun He, Nian Liu, Benyu Liu

“…ISH staining using tissue microarray (TMA) indicated that LINC02320 is associated with the clinical stage and survival rate of patients with CRC. …”
Get full text

Inhibition of ATG5-mediated autophagy maintains PMAIP1 stability to promote cell apoptosis and suppress triple-negative breast cancer progression by Fangjian Shang, Hongfeng Nie, Liyan Du, Jin Shang, Xiangquan Song, Ya Chen, Hui Li, Zhuo Wang, Yixin Qi, Liyan Zhao

“…Methods TNBC-related datasets were retrieved from The Cancer Genome Atlas and selected by Prediction Analysis of Microarray 50 analysis to assess the expression of PMAIP1 in the samples. …”
Get full text

The differentially expressed circular ribonucleic acids of primary hepatic carcinoma following liver transplantation as new diagnostic biomarkers for primary hepatic carcinoma by Weiguo Sui, Qing Gan, Fuhua Liu, Huaizhou Chen, Junfu Liu, Yong Dai

“…We chose differentially expressed targeted circular ribonucleic acids according to fold change ≥2.0 or ≤–2.0 between circular ribonucleic acids microarray of perioperative liver transplantation and normal controls. …”
Get full text

Editorial by Editorial

“…Terdapat pula kelompok pasien yang apabila diberikan kemoterapi tidak memperpanjang survival, namun justru memperpendek.9,10 Dengan penurunan cut point off reseptor estrogen dan progesteron dari 10% menjadi 1% nuclear staining, diperkirakan akan lebih banyak lagi pasien yang mendapatkan terapi hormonal.11 Apabila pemeriksaan genetic microarray digunakan sebagai prediktor maka untuk kasus kanker payudara stadium I dan II yang mengekspresikan reseptor estrogen, kemoterapi hanya berguna pada 25% kasus.10 Data ini juga menunjukkan bahwa terdapat kecenderungan pengurangan penggunaan kemoterapi dan peningkatan jumlah pasien yang hanya diberikan terapi hormonal. …”
Get full text

Establishment and Genetic Profiling of Platinum/Taxane Doublet-Resistant Cells Generated by Hybridizing Single Resistant Cells by Seiji Isonishi, Rika Saito, Noriko Yamaguchi, Saburo Saito

“…F3 and F4 acquired resistance to cisplatin and paclitaxel by 8.3/4.9- and 3.7/3.3-fold (F3/4) respectively. Microarray analysis demonstrated, out of 22284 genes, 103 genes were >4-fold up-regulated in F3/4 and 33 (32%) were identified as simultaneously upregulated genes (SUG) in C13, PX24 and F3/4. …”
Get full text

Exploration of biomarkers for predicting the prognosis of patients with diffuse large B-cell lymphoma by machine-learning analysis by Shifen Wang, Hong Tao, Xingyun Zhao, Siwen Wu, Chunwei Yang, Yuanfei Shi, Zhenshu Xu, Dawei Cui

“…To improve this situation, it is urgent to explore disease-specific diagnostic biomarkers and mechanisms. Methods Three microarray datasets (GSE25638, GSE12195 and GSE12453) were downloaded from the Gene Expression Omnibus (GEO) database. …”
Get full text

PODO447: a novel antibody to a tumor-restricted epitope on the cancer antigen podocalyxin by Blake Gilks, Martin Köbel, Diana Canals Hernaez, Michael R Hughes, Pamela Dean, Peter Bergqvist, Ismael Samudio, Ola Blixt, Katharina Wiedemeyer, Yicong Li, Chris Bond, Eric Cruz, Calvin D Roskelley, Kelly M McNagny

“…Tumor specificity was assessed using primary normal tissue and an ovarian cancer tissue microarray (TMA). Mapping of the tumor-restricted epitope was performed using enzyme-treated human tumor cell lines and a glycan array.Results One mAb (PODO447) showed strong reactivity with a variety of Podxl+ tumor cell lines but not with normal primary human tissue including Podxl+ kidney podocytes and most vascular endothelia. …”
Get full text

Long read whole genome sequencing-based discovery of structural variants and their role in aetiology of non-syndromic autism spectrum disorder in India by Jhanvi Shah, Debasrija Mondal, Deepika Jain, Priti Mhatre, Ketan Patel, Anand Iyer, Manoj Pandya, Bhargavi Menghani, Gayatri Dave, Jayesh Sheth, Frenny Sheth, Shweta Ramdas, Harsh Sheth

“…Short read sequencing (SRS) and chromosomal microarray (CMA) are unable to resolve these SVs due to their inherent technological limitations. …”
Get full text

Interferon-stimulated circHOMER1 attenuates antiviral innate immunity by Ao Zhang, Ranran Yao, Shijin Geng, Haiying Wang, Rong-Chun Tang, Hengxiang Yu, Rui Xu, Hong-Yan Chen, Yunxuan Zhou, Lan Zhang, Jun Zhang

“…These findings identify circHOMER1 as a novel interferon-stimulated circRNA that serves as a negative modulator of the antiviral innate immune response and is essential for immune homeostasis.IMPORTANCEA growing body of evidence suggests that circRNAs are involved in various physiological or pathological processes. Through circRNA microarray screening of virus-induced differentially expressed circRNAs, we identified an upregulated circRNA, circHOMER1. circHOMER1 is abundantly expressed in the brain. …”
Get full text

Evaluating IL22RA1 expression as a predictive indicator in human colon cancer progression by Xuan Yin, Renhao Geng, Junjun Chen, Bin Xu, Hui Ma, Yingting Liu, Xiao Zheng, Lujun Chen

“…Methods In this study, we utilized a multi-color immunohistochemistry (mIHC) assay to examine the expression patterns of IL22RA1, IL22, CD155, and IL22BP in a tissue microarray (TMA) comprising 90 colon cancer samples. …”
Get full text

Efficacy and tolerability of rufinamide in the treatment of epilepsy (experience of the Svt. Luka’s Institute of Child Neurology and Epilepsy) by K. Yu. Mukhin, O. A. Pylaeva, M. Yu. Bobylova, N. V. Freydkova, L. Yu. Glukhova, M. O. Abramov

“…All patients underwent electroencephalography, video-electroencephalography monitoring during wakefulness and sleep, magnetic resonance imaging (MRI) (including high-resolution MRI with special epilepsy protocols when indicated), genetic examination (tandem mass spectrometry, hereditary epilepsy gene panel test and chromosomal microarray analysis) when indicated, and laboratory tests to assess tolerability of antiepileptic drugs.Results. …”
Get full text

A first large study of whole-exome sequencing (WES) in 489 patients with suspected rare genetic disorders at a tertiary centre in Malaysia by Lip Hen Moey, Go Hun Seo, Boon Eu Cheah, Wee Teik Keng, Hane Lee, Gaik Siew Ch’ng

“…Tests offered to patients referred to genetic clinics in Malaysia include conventional karyotyping, Fragile X testing, metabolic screening and/or single gene testing. Chromosomal microarray analysis (CMA) and gene-panel testing are not routinely offered due to lack of laboratory resources. …”
Get full text

Chronic sleep deprivation induces plasma exosome-derived miR-150-5p downregulation as a novel mechanism involved in Parkinson’s disease progression by targeting DCLK1 by Li Liu, Xiangting Liu, Runze Zhang, Xiao Sun, Keping Zhang, Beibei Zheng, Ruoxi Yang, Kaiyue Yang, Guohua Song, Zhaoqiang Zhang

“…Levels of miRNA in exos were analyzed by gene expression profile microarray. Levels of miR-150-5p in exos and substantia nigra pars compacta (SNpc) were further confirmed by reverse transcription quantitative polymerase chain reaction (RT-qPCR). …”
Get full text

Identification and external validation of tumor DNA methylation panel for the recurrence risk stratification of stage II colon cancer by Tanwei Yuan, Dominic Edelmann, Víctor Moreno, Elisabeth Georgii, Lisa Barros de Andrade e Sousa, Helena Pelin, Xiaofeng Jiang, Jakob Nikolas Kather, Katrin E. Tagscherer, Wilfried Roth, Melanie Bewerunge-Hudler, Alexander Brobeil, Matthias Kloor, Hendrik Bläker, Hermann Brenner, Michael Hoffmeister

Get full text

The role of collagen type VI alpha 6 chain as a potential tumor suppressor in breast cancer: an immune regulation perspective by Jian-Di Li, Li-Li Deng, Jia-Yuan Luo, Chao-Hua Mo, Bang-Teng Chi, Wan-Ying Huang, Rong-Quan He, Di-Yuan Qin, Chang Song, Wen Zou, Gang Chen

“…Methods Immunohistochemical staining of COL6A6 was conducted on 136 breast cancer tissues and 50 non-breast-cancer controls in-house. Global microarray and high-throughput sequencing datasets were analyzed to confirm mRNA expression trends, supported by single-cell RNA sequencing for expression intensity and distribution. …”
Get full text

A retrospective analysis of 6942 amniocentesis cases by Qingsha An, Yuxiao Huang, Feifei Yu, Yilun Tao, Juan Li, Xiaoze Li

“…Among the single-method cases, Chromosomal Microarray Analysis (CMA) was the most frequently selected technique (14.18%). …”
Get full text

Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation by Jiahui Dong, Ruiping Du, Yani Zhang, Bo Hong, Gengyun Sun

“…The expressions of CAV1 and EGFR in LUAD tissues and adjacent tissues were detected by Western blotting, immunohistochemistry and microarray technology. Kaplan-Meier survival analysis and cox regression analysis were performed to verify the correlation between CAV1 expression level and the prognosis of LUAD. …”
Get full text

A Transcriptomic Approach to Sex Differences in Calcific Aortic Valve Stenosis in Patients with a Tricuspid Aortic Valve by Emma Le Nezet, MSc, Rose Tam, MSc, Zhonglin Li, MSc, Nathalie Gaudreault, BSc, Patrick Mathieu, MD, MSc, Nancy Côté, PhD, Isabelle Guisle, PhD, Sébastien Thériault, MD, MSc, Yohan Bossé, PhD, Marie-Annick Clavel, DVM, PhD

“…Methods: A total of 300 valves were collected, and genomewide gene expression was quantified using a microarray on 240. Among these, 62 female patients were matched with 62 male patients, for age (within 2 years), body mass index (within 2 kg/m2), arterial pressure (within 10/5 mm Hg), diabetes (exact), hypertension (exact), and calcific aortic valve stenosis severity. …”
Get full text