miR-9-5p/HMMR regulates the tumorigenesis and progression of clear cell renal cell carcinoma through EMT and JAK1/STAT1 signaling pathway by Xinyang Niu, Dingheng Lu, Weitao Zhan, Jiazhu Sun, Yuxiao Li, Yuchen Shi, Kai Yu, Suyuelin Huang, Xueyou Ma, Xiaoyan Liu, Ben Liu

“…Through bioinformatics analysis, we identified potential microRNAs (miRNAs) that may regulate HMMR, as well as the possible signaling pathways involved. …”
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MyD88 inhibitor TJ-M2010-5 alleviates spleen impairment and inflammation by inhibiting the PI3K/miR-136-5p/AKT3 pathway in the early infection of Trichinella spiralis by Huifang Bai, Qianqian Dang, Guoliang Chen, Lingfeng Xie, Saining Wang, Ning Jiang, Xiaoxia Wu, Shuyan Zhang, Xuelin Wang

“…Sequencing analysis showed that TJ-M2010-5 significantly up-regulated 9 and down-regulated 10 miRNAs compared with the 5 d group. A dual-luciferase reporter assay further revealed that miR-136-5p is involved in the TJ-M2010-5 treatment by targeting AKT3. …”
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The Association of Cell-Free LncH19 and miR-29b Expression with the PI3K/AKT/HIF-1/VEGF Pathway in Patients with Diabetic Nephropathy: In Silico Prediction and Clinical Validation by Noha M. Abd El-Fadeal, Basma Osman Sultan, Asmaa K. K. AbdelMaogood, Essam Al Ageeli, Fatma Tohamy Mekhamer, Sherihan Rohayem, Ahmed Shahidy, Nora Hosny, Manal S. Fawzy, Mohammed M. Ismail, Hidi A. A. Abdellatif

“…A growing body of evidence suggests that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in DN signaling pathways might serve as novel biomarkers or therapeutic targets, although this remains to be fully explored. …”
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Oral probiotic extracellular vesicle therapy mitigates Influenza A Virus infection via blunting IL-17 signaling by Hongxia Zhou, Wenbo Huang, Jieting Li, Peier Chen, Lihan Shen, Wenjing Huang, Kailin Mai, Heyan Zou, Xueqin Shi, Yunceng Weng, Yuhua Liu, Zifeng Yang, Caiwen Ou

“…Furthermore, FISH analysis detected miR-4239, one of the most abundant miRNAs in LrEVs, in both lung and intestinal tissues. …”
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miR124a-3p inhibitor alleviates AFB1-induced hepatoxicity via targeting chicken glucocorticoid receptor mRNA by Mindie Zhao, Liang Chen, Yulan Zhao, Jie Liu, Huimin Chen, Ruqian Zhao

“…However, the expression of GR and GR-targeting miRNAs in poultry after AFB1 poisoning has not been studied. …”
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MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy by Jiabin Liang, Jingxiu Zhao, Lin Yang, Qian Wang, Jing Liao, Jianhao Li, Weizhao Zhuang, Fanghong Li, Jinxian He, Yukuan Tang, Hanwei Chen, Chen Huang

“…Additionally, the differential expression of miRNAs between DSS-Exo and Exo was explored and validation of key miRNA. …”
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Integrated bioinformatics analysis to explore potential therapeutic targets and drugs for small cell carcinoma of the esophagus by Maofei Zhu, Maofei Zhu, Yueming Chu, Yueming Chu, Qiang Yuan, Qiang Yuan, Junfeng Li, Silin Chen, Lin Li, Lin Li, Lin Li

“…In addition, miRDB, miRwalk, Targetscan, OncomiR, starbase, Lncbase were used to predict miRNAs and lncRNAs that regulate hub genes, the ceRNA network was built based on this. …”
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Detection of cervical precancerous lesions and cancer by small-scale RT-qPCR analysis of oppositely deregulated mRNAs pairs in cytological smears by Anastasia A. Artyukh, Mikhail K. Ivanov, Mikhail K. Ivanov, Sergei E. Titov, Sergei E. Titov, Victoria V. Dzyubenko, Sergey E. Krasilnikov, Sergey E. Krasilnikov, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Anastasia O. Shumeikina, Nikita A. Afanasev, Anastasia V. Malek, Sergei A. Glushkov, Eduard F. Agletdinov

“…Additionally, in the same specimens, the HPV genotyping, viral load assessment, diagnosis of cervicovaginal microbiome imbalance and profiling of some relevant mRNAs and miRNAs were performed by qPCR-based methods.ResultsThe best classifiers were obtained with pairs of mRNAs whose expression changes in opposite directions during lesion progression. …”
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Regulatory Role of rno-miR-30b-5p in IL-10 and Toll-like Receptor 4 Expressions of T Lymphocytes in Experimental Autoimmune Uveitis In Vitro by Yuanyuan Sun, Dadong Guo, Bin Liu, Xuewei Yin, Huixia Wei, Kai Tang, Hongsheng Bi

“…Uveitis is a serious eye disease that usually damages young adult’s health. MicroRNAs (miRNAs) are a class of small noncoding RNAs which regulate messenger RNA (mRNA) expression. …”
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MiR-4298 and lncKRTAP5-6-3 regulated Cathepsin D expression through ERK-MAPK signaling pathway in chronic UVB-damaged HaCaT cells by Xinling Chen, Feng Zhou, Yao Lin, Yue Xia, Jie Zhang, Wenyi Hou, Yu Sun, Wei Lai, Yue Zheng

“…ObjectiveMiRNAs and lncRNAs are important regulators in the process of skin photoaging. …”
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肌球蛋白重链7基因来源的miR-208b-3p促进心肌成纤维细胞中纤维化相关基因表达 by 张梦珍, 翟琳, 郭林林, 朱杰宁, 李晖, 徐金东, 方咸宏, 单志新

“…方法通过miRNA 表达谱芯片分析18周龄的糖尿病db/db小鼠和db/m对照小鼠心肌中差异的miRNAs。通过实时荧光定量PCR（RT-qPCR）检测miR-208b-3p 在血管紧张素Ⅱ（Ang Ⅱ）和高糖/葡萄糖氧化酶（G/Go）处理的C57BL/6乳小鼠心肌细胞（NMVCs）和心肌成纤维细胞（mCFs）中的表达；利用CCK8细胞增殖实验、流式细胞术和纤维化相关蛋白（包括COL1A1、COL3A1和α-SMA）表达检测来了解miR-208b-3p对mCFs纤维化表型的影响；双萤光素酶报告基因实验检测miR-208b-3p与潜在靶基因Mtf2和Pgrmc1 3'端非翻译区（3'-UTR）的结合作用；利用RT-qPCR和Western blot 法分别检测miR-208b-3p转染的 mCFs中Mtf2 和Pgrmc1 表达；通过小干扰RNA（siRNA）抑制Mtf2和Pgrmc1表达，并检测对mCFs中纤维化相关蛋白表达的作用。…”
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Exploration of the mechanism of Lithospermum erythrorhizon oil in treating atopic dermatitis based on network pharmacology and experimental validation of the PI3K-Akt pathway regul... by Weisheng Hu, Yinlan Wang, Yingjie Zhou, Junbao Shi, Zengyan Li, Xiaoling Jiang, Qinyuan Wu, Changming Zhong, Huilan Weng, Sijie Ouyang, Yuan Jing, Xianxiang Cai, Mingda Ye, Ning Huang

“…The Gene-miRNA network suggested Lithospermum erythrorhizon oil may regulate miRNAs like hsa-mir-124-3p and hsa-let-7b-5p. Experimental results showed that Lithospermum erythrorhizon oil significantly improved AD symptoms in mice, reduced IL-4 and IL-13 levels, and decreased p-PI3K, p-PI3K/PI3K, p-Akt, and p-Akt/Akt expression, inhibiting PI3K-Akt pathway activation. …”
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Non-coding RNAs secreted by renal cancer include piR_004153 that promotes migration of mesenchymal stromal cells by Joanna Bogusławska, Małgorzata Grzanka, Piotr Popławski, Weronika Zarychta-Wiśniewska, Anna Burdzinska, Karolina Hanusek, Helena Kossowska, Roksana Iwanicka-Nowicka, Alex Białas, Beata Rybicka, Anna Adamiok-Ostrowska, Joanna Życka-Krzesińska, Marta Koblowska, Leszek Pączek, Agnieszka Piekiełko-Witkowska

“…Results RNAseq revealed 40 miRNAs, 71 tRNAs and four piRNAs that were consistently secreted by RCC cells. qPCR validation using five independent RCC cell lines confirmed that expressions of miR-10b-3p and miR-125a-5p were suppressed, while miR-365b-3p was upregulated in exosomes from RCC cells when compared with normal kidney proximal tubules. …”
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Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…This study reveals that exosomal miRNAs derived from endothelial cells exposed to catecholamine contribute to ion channel dysfunction in the setting of TTC. …”
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Transcriptomic profiles of monocyte-derived macrophages exposed to SARS-CoV-2 VOCs reveal immune-evasion escape driven by delta by Alessia Gallo, Josè Camilla Sammartino, Roberta Vazzana, Roberto Giambruno, Claudia Carcione, Nicola Cuscino, Salvatore Castelbuono, Vitale Miceli, Matteo Bulati, Daniele Lilleri, Irene Cassaniti, Pier Giulio Conaldi, Fausto Baldanti

“…Finally, we observed a significant deregulation of 3 main types of non-coding RNAs (ncRNAs): long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and small nucleolar RNAs (snoRNAs), some of which are common to coronaviruses, and some specific to SARS-CoV-2. …”
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Macrophage Notch1 signaling modulates regulatory T cells via the TGFB axis in early MASLD by Mengya Zhang, Kun Li, Xiaoxing Huang, Dongqin Xu, Ruobin Zong, Qintong Hu, Xiaoyu Dong, Qinyong Zhang, Chaochen Jiang, Yue Ge, Changyong Li, Jie Ping

“…Methods: A mouse model subjected to a short-term high-fat diet (HFD) to mimic early steatosis, along with liver biopsy samples from patients with simple steatosis, and macrophage-specific Notch1-knockout mice (Notch1M-KO), were used to investigate the role of Tregs in early MASLD and the effect of hepatic macrophage Notch1 signaling on Treg frequency. The miRNAs correlated with Treg differentiation were analyzed using exosomal miRNA sequencing. …”
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Using multiomic integration to improve blood biomarkers of major depressive disorder: a case-control studyResearch in context by Amazigh Mokhtari, El Chérif Ibrahim, Arnaud Gloaguen, Claire-Cécile Barrot, David Cohen, Margot Derouin, Hortense Vachon, Guillaume Charbonnier, Béatrice Loriod, Charles Decraene, Ipek Yalcin, Cynthia Marie-Claire, Bruno Etain, Raoul Belzeaux, Andrée Delahaye-Duriez, Pierre-Eric Lutz

“…Methods: We characterised gene expression and two mechanisms of epigenomic regulation, DNA methylation (DNAm) and microRNAs (miRNAs), in blood samples from a cohort of individuals with MDD and healthy controls (n = 169). …”
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The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats by Ailin Li, Jian Wang, Xinyue Meng, Xu Ma, Ying Liu, Hanying Li, Zhe Mo, Rui Zhang, Lei Wu, Shirui Yan, Paula Fiorella Chacon Campoverde, Gazala Zafar, Yongzheng Ma, Wenjun Que, Zhe Li, Tuo Wang, Korto Fatti Tarnue, Junrui Pei

“…In summary, fluoride has different effects on the expression levels of miRNA-200c in various biological samples of rats, and miRNAs in urine demonstrate potential as biomarkers for fluorosis.…”
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