Multiplex engineering using microRNA-mediated gene silencing in CAR T cells by Giulia Golinelli, Giulia Golinelli, John Scholler, Audrey Roussel-Gervais, Antonija Šakić, Sten Ilmjärv, Decheng Song, Khatuna Gabunia, Mei Ji, Ting J. Fan, Aasha Gupta, Mansi Deshmukh, Abdulla Berjis, Abdulla Berjis, Riccardo Cuoghi Costantini, Kimberly Apodaca, Neil C. Sheppard, Sven Kili, Massimo Dominici, Marco Alessandrini, Carl H. June, Carl H. June, Carl H. June, Bruce L. Levine

“…In vivo, S M5CAR T cells achieved tumor control with higher persistence and superior metastasis prevention. In vitro assays demonstrated enhanced resistance to alloreactive natural killer (NK) cells and peripheral blood mononuclear cells (PBMCs).ConclusionsTitratable multiplex gene silencing via targeted miRNAs offers an alternative to gene editing for CAR T cells, with potential advantages in potency, persistence, metastasis prevention, and immune evasion for allogeneic products. …”
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