Impact of pathogenic mutations of the GLUT1 glucose transporter on solute carrier dynamics using ComDYN enhanced sampling [version 2; peer review: 2 approved, 1 approved with reser... by K. Anton Feenstra, Akiko Higuchi, Kei Yura, Sanne Abeln, Halima Mouhib

“…Background: The solute carrier (SLC) family of membrane proteins is a large class of transporters for many small molecules that are vital for cellular function. …”
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Split-Cre-mediated GFP expression as a permanent marker for flagellar fusion of Trypanosoma brucei in its tsetse fly host by Ruth Etzensperger, Mattias Benninger, Berta Pozzi, Ruth Rehmann, Arunasalam Naguleswaran, Gabriela Schumann, Jan Van Den Abbeele, Isabel Roditi

“…We showed previously that a small proportion of procyclic culture forms (corresponding to insect midgut forms) can fuse their flagella and exchange cytosolic and membrane proteins. This happens reproducibly in cell culture. …”
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The expansion and loss of specific olfactory genes in relatives of parasitic lice, the stored-product psocids (Psocodea: Liposcelididae) by Jun Sun, Jian Wu, Sheng-Yuan Sun, Zhi-Dong Chen, Guy Smagghe, Jin-Jun Wang, Dan-Dan Wei

“…Result Using HMMER method and manual annotation, we have identified common gene families associated with olfactory processes, including odorant binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs). Specifically, we identified 94, 118, 26, 47, and 34 olfactory-related genes in L. bostrychophila, L. tricolor, L. entomophila, L. decolor, and L. yangi, respectively. …”
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A rare haplotype of the GJD3 gene segregating in familial Meniere’s disease interferes with connexin assembly by Alba Escalera-Balsera, Paula Robles-Bolivar, Alberto M. Parra-Perez, Silvia Murillo-Cuesta, Han Chow Chua, Lourdes Rodríguez-de la Rosa, Julio Contreras, Ewa Domarecka, Juan Carlos Amor-Dorado, Andrés Soto-Varela, Isabel Varela-Nieto, Agnieszka J. Szczepek, Alvaro Gallego-Martinez, Jose A. Lopez-Escamez

“…Conclusions The present results describe a novel association between GJD3 and FMD, providing evidence that FMD is related to changes in the inner ear channels, and supporting a new role of tectorial membrane proteins in MD.…”
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In silico development of a multi-epitope-based vaccine against Burkholderia cepacia complex using reverse vaccinology by Donya Ghorbani, Donya Ghorbani, Masoumeh Beig, Masoumeh Beig, Narjes Noori Goodarzi, Mohammad Sholeh, Mohammad Sholeh, Behzad Shahbazi, Behzad Shahbazi, Yaser Moghaddam, Farzad Badmasti

“…Highly conserved and immunogenic B-cell epitopes from outer membrane proteins (OMPs) were incorporated into a multi-epitope vaccine (MEV). …”
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Genetic determinants of silver nanoparticle resistance and the impact of gamma irradiation on nanoparticle stability by Amira M. Mahfouz, Walaa A. Eraqi, Hala Nour El Din El Hifnawi, Alaa El Din Shawky, Reham Samir, Mohamed A. Ramadan

“…Compared to the susceptible isolates, the AgNPs-resistant isolates St.L_R.Ag and KP_R.Ag had unique mutations in specific efflux pump systems, stress response, outer membrane proteins, and permeases. These findings might help to explain how single-nucleotide variants contribute to AgNPs resistance. …”
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Development and utilization of Treponema pallidum expressing green fluorescent protein to study spirochete-host interactions and antibody-mediated clearance: expanding the toolbox... by Kristina N. Delgado, Crystal F. Vicente, Christopher M. Hennelly, Farhang Aghakhanian, Jonathan B. Parr, Kevin P. Claffey, Justin D. Radolf, Kelly L. Hawley, Melissa J. Caimano

“…In the study, we generated and characterized an infectious TPA strain that constitutively expresses green fluorescent protein and used this strain to visualize the interaction of TPA with host cells and functionally characterize antibodies directed against treponemal outer membrane proteins. Most notably, we assessed the ability of surface-bound antibodies to inhibit the growth of TPA in vitro and/or disrupt the spirochete’s fragile outer membrane. …”
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Generation of novel oncolytic vaccinia virus with improved intravenous efficacy through protection against complement-mediated lysis and evasion of neutralization by vaccinia virus... by Dong-Sup Lee, Mi-Ju Park, Namhee Lee, Yun-Hui Jeon, Jiyoon Yoo, Suk-kyung Shin, Songyi Lee, Byung-Jin Jung, Yun-Kyoung Hong, Keunhee Oh

“…Here, we generated SJ-600 series oncolytic vaccinia viruses that can mimic complement-resistant extracellular enveloped virions by incorporating human CRP CD55 on the intracellular mature virion (IMV) membrane.Methods The N-terminus of the human CD55 protein was fused to the transmembrane domains of the six type I membrane proteins of the IMV; the resulting recombinant viruses were named SJ-600 series viruses. …”
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