Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease by S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina

“…Two genes controlling hulled vs naked (Nud) and two-rowed vs six-rowed barley (Vrs1) were used as targets to be modifed via polyethyleneglycol-mediated cellular uptake of guide-RNA/Cas9-encoding plasmid DNA. …”
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<i>Zanthoxylum piperitum</i> Benn. Attenuates Monosodium Urate-Induced Gouty Arthritis: A Network Pharmacology Investigation of Its Anti-Inflammatory Mechanisms by Sung Wook Kim, Soo Hyun Jeong, Jong Uk Kim, Mi Hye Kim, Wonwoong Lee, Cheol-Jung Lee, Tae Han Yook, Gabsik Yang

“…The anti-inflammatory effects of deZP are believed to be mediated through the NLRP3 inflammasome pathway, as indicated by RNA sequencing and network pharmacology analyses. …”
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Influence of the polysaccharide capsule on virulence and fitness of Klebsiella pneumoniae by Lisa Zierke, Rodi Mourad, Thomas P. Kohler, Mathias Müsken, Sven Hammerschmidt

“…In whole blood killing assays, we demonstrate that the K. pneumoniae capsule is essential for survival in human blood, protecting K. pneumoniae against recognition and clearance by the human immune system, as well as complement-mediated opsonization and killing. The non-encapsulated mutant, in contrast, was unable to survive in either whole blood or human plasma. …”
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Lysine Reacts with Cholesterol Hydroperoxide to Form Secosterol Aldehydes and Lysine-Secosterol Aldehyde Adducts by George Wafula Wanjala, Arnold Nola Onyango, David Rasugu Abuga, John Kamathi Muchuna, Calvin Onyango, Moses Makayoto

“…We propose that the amide type adducts, which are major biomarkers of lipid oxidation, are mainly formed by singlet oxygen-mediated oxidation of the carbinolamine adducts.…”
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Genetic disruption of Blimp-1 drastically augments the antitumor efficacy of BCMA-targeting CAR T cells by Anthony M. Battram, Joan Mañé-Pujol, David F. Moreno, Aina Oliver-Caldés, Judit Carpio, Oriol Cardus, Luis Gerardo Rodríguez-Lobato, Álvaro Urbano-Ispizua, Carlos Fernández de Larrea

“…To improve long-term efficacy, we used CRISPR/CRISPR-associated protein 9-mediated gene editing to ablate the expression of the transcription factor Blimp-1. …”
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Transcriptional regulatory network analysis identifies GRN as a key regulator bridging chemotherapy and immunotherapy response in small cell lung cancer by Seungyeul Yoo, Ayushi S. Patel, Sarah Karam, Yi Zhong, Li Wang, Feng Jiang, Ranran Kong, Sharon Bikvan, Wenhui Wang, Abhilasha Sinha, Charles A. Powell, Jun Zhu, Hideo Watanabe

“…Overall, our findings implicate GRN as a bridge between chemotherapy and immunotherapy resistance through GRN-mediated mechanisms.…”
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Renal glomerular and tubular injury in the offspring of the preeclampsia-like syndrome by Yong Wang, Hao Wang, Huiqing Lu, Ji Ma, Wei Wu, Yinan Wang, Bo Ma, Hao Zhu, Rong Hu

“…Preeclampsia induced by L-NAME results in increased blood pressure and renal damage in offspring, potentially mediated by early alterations in the renal RAS system. …”
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Microbiota, parasitic infections and their relationship with nutritional status and neurocognitive functioning in children from Ecuador—Proyecto Guagua: research protocol for a cro... by Sandra Victoria Abril-Ulloa, Tannia Valeria Carpio-Arias, Renata Alejandra Alvarado-Barba, Cristina Gabriela Ríos-Romero, Pamela Vinueza-Veloz, Sueny Paloma Lima-dos-Santos, Sueny Paloma Lima-dos-Santos, Igor Eduardo Astudillo-Skliarova, Igor Eduardo Astudillo-Skliarova, Ruth Irene Arias-Gutiérrez, Manuel Pérez-Quintana, Henk-Jan Boele, Henk-Jan Boele, Henk-Jan Boele, María Fernanda Vinueza-Veloz

“…We also plan to test the hypothesis that an altered microbiota mediates the influence of malnutrition on neurocognitive functioning and parasitosis.Ethics and disseminationProyecto Guagua received ethical approval from the Ethics Committee of Universidad de Cuenca in July 2022. …”
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Lower urinary dysfunction as a long-term effect of childhood vincristine treatment, with potential influences by sex and dose by Nao Iguchi, Ali Teimouri, Duncan T. Wilcox, Anna P. Malykhina, Nicholas G. Cost

“…Noteworthy functional changes were observed in bladder strips from the VCR group, including decreased nerve-mediated contraction, heightened contractile responses to cholinergic and purinergic agonists, enhanced responsiveness to histamine—primarily via histamine receptor 1 (Hrh1)—and an augmented relaxation effect with compound 48/80 (a mast cell degranulator), relative to the control group. …”
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Retinal Levels of Amyloid Beta Correlate with Cerebral Levels of Amyloid Beta in Young APPswe/PS1dE9 Transgenic Mice before Onset of Alzheimer’s Disease by Xi Mei, Mengxiang Yang, Lina Zhu, Qi Zhou, Xingxing Li, Zhongming Chen, Chenjun Zou

“…With increasing age, Aβ-mediated degenerative change in the cerebrum (P<0.001 in 5 months) and retina (P<0.01 in 5 months) increased significantly. …”
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Poplar transformation with variable explant sources to maximize transformation efficiency by Haiwei Lu, Sara Jawdy, Jin-Gui Chen, Xiaohan Yang, Udaya C. Kalluri

“…Abstract For decades, Agrobacterium tumefaciens-mediated plant transformation has played an integral role in advancing fundamental and applied plant biology. …”
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Semen extender triggers a mild physiological inflammatory response in the uterus without disrupting sperm-uterine immune crosstalk in vitro in cattle by Malinda HULUGALLA, Alireza MANSOURI, Elham WAEHAMA, Ihshan AKTHAR, Akio MIYAMOTO

“…These findings demonstrate that egg yolk-based SE, independent of sperm, triggers a mild physiological inflammatory response mediated by the TLR2/1 and NOD1 signaling pathways. …”
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Hypertension and retinal microvascular dysfunction (HyperVasc): protocol of a randomised controlled exercise trial in patients with hypertension by Justin Carrard, Lukas Streese, Timo Hinrichs, Henner Hanssen, Arno Schmidt-Trucksäss, Konstantin Gugleta, Joséphine Gander, Christoph Hauser

“…Further outcomes will be other retinal microvascular biomarkers, flow-mediated dilation of the brachial artery as well as blood pressure, cardiorespiratory fitness, microalbuminuria, hypertensive retinopathy and classical CV risk markers. …”
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Cross-Talk between NOK and EGFR: Juxtamembrane and Kinase domain interactions enhancing STAT3/5 signaling in breast cancer tumorigenesis by Yinyin Wang, Bingdong Zhang, Chunhua He, Bo Tian, Sihan Liu, Jianghua Li, Jiayu Wang, Shigao Yang, Bingtao Zhu, Xiaoguang Wang, Zhijie Chang, Chenxi Cao

“…Hence, investigating the diverse partnership profiles of EGFR is crucial for elucidating the mechanisms underlying EGFR-mediated actions in tumors, which in turn can guide the development of targeted therapeutic strategies. …”
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Evolution of the RNA alternative decay cis element into a high-affinity target for the immunomodulatory protein Roquin by Jan-Niklas Tants, Katharina Friedrich, Jasmina Neumann, Andreas Schlundt

“…The Ox40 3’UTR comprises both a CDE and ADE, each sufficient for Roquin-mediated control. Opposed to highly conserved and abundant CDE structures, ADEs are rarer, but predicted to exhibit a greater structural heterogeneity. …”
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Limb Position Influences Peripheral Arterial Stiffness Reduction with Reactive Hyperemia by Ronald E. Jackson, Natalia S. Lima, Sara R. Sherman, Brooks A. Hibner, Philip S. Clifford

“…Abstract The mechanism behind the acute reduction in peripheral arterial stiffness with reactive hyperemia is presumed to be flow-mediated; however, this has not been clearly demonstrated. …”
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Overexpression of OsDUF868.12 enhances salt tolerance in rice by Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li, Lihua Li

“…A series of experiments, including RT-qPCR, Agrobacterium-mediated transient transformation in tobacco for localization analysis, phenotypic characterization, physiological and biochemical index measurement, and leaf staining, were conducted to investigate the function of OsDUF868.12 under salt stress. …”
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Septuple XBi2Te4 (X=Ge, Sn, Pb) intercalated MnBi2Te4 for realizing interlayer ferromagnetism and quantum anomalous hall effect by Ruixia Yang, Xiaoxiao Man, Jiahui Peng, Jingjing Zhang, Fei Wang, Fang Wang, Huisheng Zhang, Xiaohong Xu

“…Using first-principles calculations, we demonstrate that the p z orbital of the X atom mediates interactions between interlayer Mn atoms, enabling FM coupling. …”
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Physiology‐based pharmacokinetic model with relative transcriptomics to evaluate tissue distribution and receptor occupancy of anifrolumab by Pradeep Sharma, David W. Boulton, Lynn N. Bertagnolli, Weifeng Tang

“…Here, we built a population physiology‐based pharmacokinetic (PBPK) model of anifrolumab by utilizing the physiochemical properties of anifrolumab, binding kinetics to the Fc gamma neonatal receptor, and target‐mediated drug disposition properties. A novel relative transcriptomics approach was employed to determine IFNAR1 expression in tissues (blood, skin, gastrointestinal tract, lungs, and muscle) using mRNA abundances from bioinformatic databases. …”
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Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe by Rika Indri Astuti, Muhammad Eka Prastya, Irmanida Batubara, Eka Budiarti, Aulia Ilmiyawati

“…Interestingly, S. nodiflora water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in S. pombe. …”
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