Development and validation of a LAMP-based method for rapid and reliable detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald by Moutoshi Chakraborty, Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Shamsul Arafin Bhuiyan, Simon Strachan, Simon Strachan, Muhammad J. A. Shiddiky, Nam-Trung Nguyen, Rebecca Ford, Rebecca Ford

“…Sugarcane samples from infected plants were subjected to heat lysis, followed by loop-mediated isothermal amplification (LAMP)-based fluorescence and colorimetric quantification within a single microcentrifuge tube.ResultsThe method exhibited exceptional detection sensitivity (detecting as low as 1 cell/μL), reproducibility [with a standard deviation (SD) of <5% for n = 3], and a broad linear dynamic range (10 pM to 1 aM or 107–100 copies/μL, r = 0.99). …”
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Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human Pancreatic β Cells by Akiyo Yamauchi, Asako Itaya-Hironaka, Sumiyo Sakuramoto-Tsuchida, Maiko Takeda, Kiyomi Yoshimoto, Tomoko Miyaoka, Takanori Fujimura, Hiroki Tsujinaka, Chikatsugu Tsuchida, Hiroyo Ota, Shin Takasawa

“…Furthermore, small interfering RNA-mediated targeting of STAT3 blocked the IL-6+Dx-induced expression of REG Iα and REG Iβ. …”
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Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells by So Young Kim, Seung Ha Oh, Jun Ho Lee, Myung-Whan Suh, Moo Kyun Park

“…The PGE2, TGFβ, and VEGF were the mediators of the anti-inflammatory effects of PL-MSCs.…”
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Structural characterization of two γδ TCR/CD3 complexes by Mohammed Hoque, John Benji Grigg, Trudy Ramlall, Jennifer Jones, Luke L. McGoldrick, John C. Lin, William C. Olson, Eric Smith, Matthew C. Franklin, Tong Zhang, Kei Saotome

“…Furthermore, we observed that the Vγ5 Vδ1 complex forms a TCR γ5 chain-mediated dimeric species whereby two TCR/CD3 complexes are assembled. …”
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High rate of false positive malaria rapid diagnostic tests in a district hospital in Uganda by Susanne E. Mortazavi, Susanne E. Mortazavi, Allan Lugaajju, Anna-Clara Ivarsson, Sara Karlsson Söbirk, Sara Karlsson Söbirk, Hans Norrgren, Hans Norrgren, Kristina E. M. Persson, Kristina E. M. Persson

“…This study aimed to evaluate the diagnostic accuracy of RDT compared to microscopy and loop-mediated isothermal amplification (LAMP) in a malaria-endemic region of Uganda.MethodsA prospective study was conducted with 225 febrile individuals diagnosed with malaria using RightSign Malaria Ag HRPII/Pan Plasmodium Aldolase RDT (Hangzhou Biotest Biotech Co., Ltd., China) at Iganga General Hospital, Uganda. …”
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Chemotherapy in synergy with innate immune agonists enhances T cell priming for checkpoint inhibitor treatment in pancreatic cancer by Nan Niu, Keyu Li, Junke Wang, Vanessa Funes, Birginia Espinoza, Pan Li, Jianxin Wang, Melissa Lyman, Mengni He, Brian Herbst, Michael Wichroski, Ruslan Novosiadly, Sami Shoucair, Yiping Mou, Lei Zheng

“…RNA sequencing analysis of the pancreatic tumors demonstrates the role of the combination of STING/NLRP3 agonist and chemotherapy, but not either treatment modality alone, in upregulating the T cell activation signaling. The NLRP3 agonist-mediated T cell activation is likely through regulating the nitrogen metabolism pathways. …”
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Longitudinal assessment of thyroid function in dogs with hypoadrenocorticism: Clinical outcomes and prevalence of autoantibodies by Nadja S. Sieber‐Ruckstuhl, Barbara Riond, Federico Fracassi, Claudia Kuemmerle‐Fraune, Solène Meunier, Regina Hofmann‐Lehmann, Claudia E. Reusch, Claudia Mueller, Natalie Hofer‐Inteeworn, Felicitas S. Boretti

“…Abstract Background Knowledge about primary hypoadrenocorticism coexisting with immune‐mediated thyroiditis (Schmidt's syndrome) in dogs is limited. …”
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Sevuparin strongly reduces hepcidin expression in cells, mice, and healthy human volunteers by Michela Asperti, Andrea Denardo, Magdalena Gryzik, Kristina E. M. Persson, Göran Westerberg, John Öhd, Maura Poli

“…Abstract Hepcidin is an essential regulator of systemic iron availability mediating both iron uptake from the diet and its release from body stores. …”
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Genetic predisposition to altered blood cell homeostasis is associated with glioma risk and survival by Linda Kachuri, Geno A. Guerra, Taishi Nakase, George A. Wendt, Helen M. Hansen, Annette M. Molinaro, Paige Bracci, Lucie McCoy, Terri Rice, John K. Wiencke, Jeanette E. Eckel-Passow, Robert B. Jenkins, Margaret Wrensch, Stephen S. Francis

“…Our findings highlight immune-mediated susceptibility mechanisms with potential disease management implications.…”
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A guide for the generation of repositories of clinical samples for research on Chagas disease. by Nieves Martínez-Peinado, Juan Carlos Gabaldón-Figueira, Roberto Rodrigues Ferreira, María Carmen Thomas, Manuel Carlos López, Tania Cremonini Araújo-Jorge, Belkisyolé Alarcón de Noya, Soledad Berón, Janine Ramsey, Irene Losada Galván, Alejandro Gabriel Schijman, Adriana González Martínez, Andrés Mariano Ruiz, Gimena Rojas, Roberto Magalhães Saraiva, Oscar Noya-González, Andrea Gómez, Rosa A Maldonado, Jimmy Pinto, Faustino Torrico, Ivan Scandale, Fernán Agüero, María-Jesús Pinazo, Joaquim Gascón, Alejandro Marcel Hasslocher-Moreno, Julio Alonso-Padilla, NHEPACHA Network (New Tools for the Diagnosis and Evaluation of Chagas Disease)

“…New diagnostic methods, such as rapid tests (RDTs) to diagnose chronic Chagas disease, or loop-mediated isothermal amplification (LAMP), to detect acute infections, represent valuable alternatives, but the parasite's remarkable genetic diversity might make its implementation difficult. …”
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Transcriptional and Posttranscriptional Regulations of the HLA-G Gene by Erick C. Castelli, Luciana C. Veiga-Castelli, Layale Yaghi, Philippe Moreau, Eduardo A. Donadi

“…The HLA-G promoter region is different from the classical class I promoters, mainly because (i) it lacks regulatory responsive elements for IFN-γ and NF-κB, (ii) the proximal promoter region (within 200 bases from the first translated ATG) does not mediate transactivation by the principal HLA class I transactivation mechanisms, and (iii) the presence of identified alternative regulatory elements (heat shock, progesterone and hypoxia-responsive elements) and unidentified responsive elements for IL-10, glucocorticoids, and other transcription factors is evident. …”
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Impact of sleep deprivation on colon cancer: Unraveling the KynA-P4HA2-HIF-1α axis in tumor lipid metabolism and metastasis by Zuojie Peng, Jia Song, Wenzhong Zhu, Haijun Bao, Yuan Hu, Yongping Shi, Xukai Cheng, Mi Jiang, Feifei Fang, Jinhuang Chen, Xiaogang Shu

“…Subsequently, mice feces were collected for untargeted metabolomics to screen and identify the key mediator, Kynurenic acid (KynA). Furthermore, HILPDA was screened by transcriptomics, and its potential mechanism was explored through ChIP, co-IP, ubiquitination experiments, phenotyping experiments, etc. …”
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The guanine nucleotide exchange factor DOCK5 negatively regulates osteoblast differentiation and BMP2-induced bone regeneration via the MKK3/6 and p38 signaling pathways by Ju Ang Kim, Soomin Im, Jiwon Lim, Jung Min Hong, Hye Jung Ihn, Jong-Sup Bae, Jung-Eun Kim, Yong Chul Bae, Eui Kyun Park

“…Abstract DOCK5 (dedicator of cytokinesis 5), a guanine nucleotide exchange factor for Rac1, has been implicated in BMP2-mediated osteoblast differentiation, but its specific role in osteogenesis and bone regeneration remained unclear. …”
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Effects of Melatonin and Epiphyseal Proteins on Fluoride-Induced Adverse Changes in Antioxidant Status of Heart, Liver, and Kidney of Rats by Vijay K. Bharti, R. S. Srivastava, H. Kumar, S. Bag, A. C. Majumdar, G. Singh, S. R. Pandi-Perumal, Gregory M. Brown

“…Several experimental and clinical reports indicated the oxidative stress-mediated adverse changes in vital organs of human and animal in fluoride (F) toxicity. …”
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Oral Health Impact Profile in Celiac Patients: Analysis of Recent Findings in a Literature Review by Gabriele Cervino, Luca Fiorillo, Luigi Laino, Alan Scott Herford, Floriana Lauritano, Giuseppe Lo Giudice, Fausto Famà, Rossella Santoro, Giuseppe Troiano, Gaetano Iannello, Marco Cicciù

“…The celiac disease (CD) is a chronic immune-mediated disorder triggered by the ingestion of gluten that appears in genetically predisposed patients. …”
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Impacts of stigma and discrimination on people with obesity who smoke cigarettes by Liza A. Kolbasov, Arryn A. Guy, Cara M. Murphy

“…Those who reported more internalization of weight bias and more everyday discrimination reported greater depressive symptomatology and greater concern about gaining weight while quitting smoking, with depressive symptomatology fully mediating both internalization of weight bias and everyday discrimination’s relation with concern about gaining weight while quitting smoking. …”
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Oncolytic Viruses and Immunotherapy for the Treatment of Uveal Melanoma and Retinoblastoma: The Current Landscape and Novel Advances by Merve Kulbay, Nicolas Tuli, Massimo Mazza, Armaan Jaffer, Sarinee Juntipwong, Emily Marcotte, Stuti Misty Tanya, Anne Xuan-Lan Nguyen, Miguel N. Burnier, Hakan Demirci

“…To overcome these obstacles, immunotherapy, which encompasses different treatment options such as oncolytic viruses, antibody-mediated immune modulations, and targeted immunotherapy, has shown great potential as a novel therapeutic tool for cancer therapy. …”
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Work-related well-being in early career: A role of self-compassion by Kristýna Krejčová, Igor Krejčí, Hana Chýlová, Pavla Rymešová, Pavel Michálek

“…Structural equation modeling indicated that workaholism was a mediator between the negative scales of self-compassion and work engagement with job boredom. …”
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Ranakinestatin-PPF from the Skin Secretion of the Fukien Gold-Striped Pond Frog, Pelophylax plancyi fukienensis: A Prototype of a Novel Class of Bradykinin B2 Receptor Antagonist P... by Jie Ma, Yu Luo, Lilin Ge, Lei Wang, Mei Zhou, Yingqi Zhang, Jinao Duan, Tianbao Chen, Chris Shaw

“…Using a preconstricted preparation of rat tail arterial smooth muscle, a single dose of 10−6 M of the peptide effectively inhibited the dose-dependent relaxation effect of bradykinin between 10−11 M and 10−5 M and subsequently, this effect was pharmacologically-characterized using specific bradykinin B1- (desArg-HOE140) and B2-receptor (HOE140) antagonists; the data from which demonstrated that the antagonism of the novel peptide was mediated through B2-receptors. Ranakinestatin—PPF—thus represents a prototype of an amphibian skin peptide family that functions as a bradykinin B2-receptor antagonist herein demonstrated using mammalian vascular smooth muscle.…”
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Research hotspots and trends in the antioxidant effects of acupuncture: A bibliometric analysis from 2003 to 2024 by Zhilin Huang, Song Li, YiHao Zhou, Anhong Dai, Yi Zhang, Meifang Liu, Mengqi Le, Jing Shi

“…Objective: Oxidative stress is an important factor mediating the pathologic progression of many diseases. …”
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