An Emerging Role for IQGAP1 in Regulating Protein Traffic by Mahasin Osman

“…Recent evidence suggests that IQGAP1 plays its role in cell growth through regulating the conserved mTOR pathway. The mTOR signaling cascade has been implicated in membrane traffic and is activated in nearly all human cancers, but clinical response to the mTOR-specific inhibitor rapamycin has been disappointing. …”
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Comparing the Prognostic Value of PTEN and Akt Expression with the Mitotic Activity Index in Adjuvant Chemotherapy-Treated Node-Negative Breast Cancer patients aged <55 years by Emiel A. M. Janssen, Håvard Søiland, Ivar Skaland, Einar Gudlaugson, Kjell H. Kjellevold, Arne Nysted, Jon-Arne Søreide, Jan P. A. Baak

“…Background: The prognostic value of the PI3K/Akt/mTOR pathway and PTEN in invasive breast cancer (IBC) is controversial. …”
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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…Western blots confirmed that the expression of PI3K/Akt/mTOR pathway-related proteins (p-PI3K, p-AKT, and mTOR) in BC cells was decreased after knocking down SAE1. …”
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Protocol for microinjection of rapamycin into the zebrafish habenula by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

“…Summary: Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. …”
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ET-1通过上调CXCR4表达促进鼻咽癌低转移 细胞株6-10B的迁徙

“…【目的】 探讨内皮素-1(ET-1)上调CXCR4表达促进鼻咽癌低转移细胞株6-10B迁徙的机制【方法】 加入不同浓度的ET-1以及同时加入SDF-1α处理6-10B细胞,趋化实验检测ET-1对6-10B细胞迁徙能力的影响分别采用Real-time PCR以及Western Blot方法检测ET-1上调后CXCR4在基因以及蛋白水平的变化Western Blot方法检测用特异性的ETAR抑制剂或者PI3K/AKT/mTOR或MARK1/ERK1/2抑制剂预处理6-10细胞后,ET-1对CXCR4表达水平的变化;并探讨ET-1处理后,PI3K/AKT/mTOR以及MARK1/ERK1/2信号通路中蛋白磷酸化水平的改变【结果】 趋化实验显示6-10B细胞丧失对SDF-1α的趋化能力,但不同浓度的ET-1刺激后6-10B细胞对SDF-1α的迁徙能力明显增强(P < 0.05),10 nmol/L ET-1的作用最明显Real-time PCR以及Western Blot结果显示,不同浓度的ET-1处理6-10B细胞后,ET-1在基因以及蛋白的水平可以呈剂量依赖性及时间依赖性地上调CXCR4的表达用特异性的内皮素受体A(ETAR)抑制剂或者PI3K/AKT/mTOR或MARK1/ERK1/2通路的抑制剂,能够明显抑制ET-1对CXCR4表达的上调Western blot检测显示ET-1能激活PI3K/AKT/mTOR及MAPK1/ERK1/2信号通路【结论】 ET-1能上调6-10B细胞功能性CXCR4的mRNA和蛋白表达,并能明显增强6-10B细胞对SDF-1α的迁徙能力CXCR4的上调主要是通过ETAR介导,激活PI3K/AKT/mTOR和MAPK1/ERK1/2信号通路…”
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Differential Role of Rapamycin and Torin/KU63794 in Inflammatory Response of 264.7 RAW Macrophages Stimulated by CA-MRSA by Rebekah K. H. Shappley, Thomas Spentzas

“…There is a differential response between Rapamycin (mTOR-1 inhibitor) and Torin KU63794 (mTOR 1 and 2 inhibitors). …”
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Molecular mechanisms of autism as a form of synaptic dysfunction by E. A. Trifonova, T. M. Khlebodarova, N. E. Gruntenko

“…Normally mTOR-regulated translation is slightly inhibited, and in most cases mutational damage to at least one of the links of the mTOR signaling pathway, increases translation and leads to impaired synaptic plasticity and behavior. …”
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MFAP5 inhibits the malignant progression of endometrial cancer cells in vitro by Liang Guanying, Qi Zijuan, Du Chun

“…A western blot was used to analyze the expression of markers affiliated with the epithelial–mesenchymal transition process and AKT/mTOR pathway. As a result, MFAP5 was found to be down-regulated in EC. …”
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卡博替尼联合PD-L1单抗对黑色素瘤小鼠移植瘤的抑制作用机制 by 祝保艳, 李婧婧, 李丹丹, 文习之, 黄复雪, 刘巍, 张晓实

“…Western blot检测显示联合组及卡博替尼组对AKT和mTOR蛋白水平无明显影响，但均可下调p-AKT和p-mTOR蛋白水平，联合组作用更为显著。【结论】卡博替尼联合PD-L1单抗有协同抗肿瘤作用，这种协同抗肿瘤作用机制可能是通过促进B16-F10细胞凋亡及抑制AKT/mTOR通路所致。…”
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雷帕霉素逆转卵巢癌细胞系SKOV3/DDP顺铂耐药及其机制探讨 by 徐国才, 王东雁, 卢淮武, 林仲秋, 张丙忠

“…【结论】雷帕霉素对卵巢癌顺铂耐药细胞系SKOV3/DDP具有耐药逆转作用，其机制可能为通过抑制 Akt/mTOR通路中mTOR及下游相关蛋白表达进而抑制耐药细胞的增殖并促进其凋亡进程。…”
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Bevacizumab Demonstrates Prolonged Disease Stabilization in Patients with Heavily Pretreated Metastatic Renal Cell Carcinoma: A Case Series and Review of the Literature by Nicole M. Agostino, Rebecca Gingrich, Joseph J. Drabick

“…We report our experience in 4 patients with metastatic RCC who had failed prior cytokine, TKI, and mTOR inhibitors who were treated with bevacizumab as single agent therapy. …”
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The molecular view of mechanical stress of brain cells, local translation, and neurodegenerative diseases by T. M. Khlebodarova

“…Evidence is provided that chronic mechanical stress may result in aberrant activation of mTOR signaling and the WAVE regulatory complex via the YAP/TAZ system, the key sensor of mechanical signals, and influence the associated pathways regulating the formation of F actin filaments and the dendritic spine structure. …”
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Neuronal subset-specific Pten-deficient mice do not exhibit deficits in sensorimotor gating processes [version 4; peer review: 1 approved, 2 approved with reservations] by Matthew S. Binder, Joaquin N. Lugo, Suzanne O. Nolan

“…NS-Pten KO mice exhibit hyperactivity of the PI3K/AKT/mTOR signaling pathway which is implicated in the onset of autistic deficits. …”
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The Role of Targeted Therapy in Metastatic Renal Cell Carcinoma by Jaya Unnithan, Brian I. Rini

“…Temsirolimus is an mTOR inhibitor that leads to G1 cell cycle arrest and may affect VEGF production. …”
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NOP2/Sun RNA Methyltransferase 4 Regulates the Mammalian Target of Rapamycin Signaling Pathway to Promote Hepatocellular Carcinoma Progression by Congren Wang, Shaoying Ke, Shaoze Lin, Conglin Lin, Zhibing Cai, Lingju Hong, Qunxiong Pan

“…The western blot was utilized to determine the protein expression level of NSUN4 and mammalian target of rapamycin (mTOR) pathway-related proteins in cells and mouse tumor tissues. …”
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Neuronal subset-specific Pten-deficient mice do not exhibit deficits in sensorimotor gating processes [version 3; peer review: 1 approved, 2 approved with reservations] by Matthew S. Binder, Joaquin N. Lugo, Suzanne O. Nolan

“…NS-Pten KO mice exhibit hyperactivity of the PI3K/AKT/mTOR signaling pathway which is implicated in the onset of autistic deficits. …”
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Improvement in Impaired Social Cognition but Not Seizures by Everolimus in a Child with Tuberous Sclerosis-Associated Autism through Increased Serum Antioxidant Proteins and Oxidan... by Kunio Yui, George Imataka, Hitomi Sasaki, Yohei Kawasaki, Tohru Okanshi, Ryoichi Shiroki, Shigemi Yoshihara

“…We investigated the effect of the mammalian target of rapamycin (mTOR) inhibitor everolimus on tuberous sclerosis complex- (TSC-) associated autistic symptoms and focal seizures with impaired awareness in a female child with TSC. …”
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Lycium barbarum polysaccharide inhibits retinal neovascularization and inflammation in vitro and in vivo by Bin Wu, Xue Wang, Wei Zhang, Xin Wang, Qi-Miao Wang, Ya-Ju Pang, Yi-Chun Kong

“…The expression of epidermal growth factor receptor (EGFR), phosphatidylinositol 3-kinase (PI3K), mammalian target of rapamycin (mTOR), phosphorylated mammalian target of rapamycin (p-mTOR), protein kinase B (AKT), phosphorylated protein kinase B (p-AKT), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), and tumor necrosis factor-α (TNF-α) in each group were analyzed by Western blot. …”
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Promising Response with PI3K Inhibitor for a Patient with Heavily Pretreated PIK3CA Mutation Head-and-Neck Cancer by Ming-Jing Lee, Hsiang-Fong Kao

“…Several pathways have been identified in HNSCC, including the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. The PI3K/AKT/mTOR pathway is frequently dysregulated in various cancers due to activating mutations or amplification of PIK3CA. …”
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The Synergistic Effects of Celastrol in combination with Tamoxifen on Apoptosis and Autophagy in MCF-7 Cells by Lijun Wang, Luqun Tang, Chengyun Yao, Chunyan Liu, Yongqian Shu

“…Furthermore, the decrease in p-Akt and p-mTOR in MCF-7 cells, along with the increase in the autophagy marker proteins LC3II and P62, suggested that the Akt/mTOR pathway might be involved in the triggering of cell autophagy by the combination treatment. …”
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