丙酮酸乙酯抑制脓毒症时HMGB1释放的分子机制

“…36和48 h,LPS+ EP组细胞内HMGB1 mRNA表达比LPS组明显减少;在LPS和LPS+ EP刺激后18~48h,LPS+ EP组培养上清中HMGB1蛋白含量明显低于LPS组?…”
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水蛭素对大鼠急性心肌梗死后室性心律失常的影响

“…【目的】 探讨凝血酶的直接抑制剂重组双功能水蛭素对大鼠急性心肌梗死后室性心律失常的影响及相关机制65377;【方法】 将70只雄性SD大鼠随机分为10组:水蛭素(HIR)结扎前(HIR 0 min)组,HIR结扎后5 min(HIR 5 min)组, HIR结扎后10 min(HIR 10 min)组,HIR结扎后20 min(HIR 20 min)组,HIR结扎后30 min(HIR 30 min)组),和生理盐水(NS)对应的各时间组,即NS 0 min,NS 5 min,NS 10 min,NS 20 min,NS 30 min,最终每组7只65377;观察各组室性心律失常的发生情况,Evans法计量各组心梗面积及采用逆转录聚合酶链反应对各组缺血心肌组织的IP3R家族的三种亚型进行测定65377; 【结果】 水蛭素组发生室性心律失常的持续时间及心律失常评分较生理盐水组在结扎后5 ~ 20 min均减少(P < 0.05);IP3R家族的三种亚型均与心梗后室性心律失常持续时间呈正相关性;但IP3R2 mRNA在结扎后10 min及IP3R3 mRNA在结扎后10 min和20 min,HIR组较NS组下调(P < 0.05)65377; 【结论】 水蛭素有抗心梗后室性心律失常的发生作用,其机制可能为通过IP3R2和IP3R3实现的,而并非IP3R165377;…”
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马鹿<italic>MORF4L2</italic>组织表达、基因克隆及生物信息学分析 by 韩青, 高仰, 吴玄烨, 索婧媛, 金庆梅, 刘学东

“…为了探究马鹿（<italic>Cervus elaphus</italic>）鹿茸中MORF4L2所发挥的功能，使用qPCR技术检测鹿茸不同组织中<italic>MORF4L2</italic>基因的表达水平，采用PCR克隆马鹿<italic>MORF4L2</italic>基因的CDS序列，通过多物种比对<italic>MORF4L2</italic>基因mRNA序列进行相似性分析并构建系统进化树，利用生物信息学方法预测分析<italic>MORF4L2</italic>编码蛋白的结构与理化性质。…”
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Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation by Yanqin Wang, Xinxin Li, Xiaoyun Xing, Haibo Xue, Ruiqun Qi, Hong Ji, Lei Ma

“…Quantitative real-time RT-PCR detected Hes1 mRNA expression. Enzyme-linked immunosorbent assay and western blot measured IL-17A serum concentration and protein expression. …”
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肝癌细胞株Bel-7402中侧群细胞的分离和致瘤能力 by 陈规划, 曾宪成

“…(5.1 ± 0.9)%,两者差异明显(P < 0.05)?SP细胞ABCG2 mRNA和CD133 mRNA平均表达水平分别是NSP细胞的3.03倍(P < 0.01)和1.98倍(P < 0.01)?…”
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A New Way for Beta Cell Neogenesis: Transdifferentiation from Alpha Cells Induced by Glucagon-Like Peptide 1 by Zhen Zhang, Yinghui Hu, Ningning Xu, Wenjun Zhou, Lei Yang, Rongping Chen, Rui Yang, Jia Sun, Hong Chen

“…The regulation on the GLP1 receptor and its downstream transcription factor PI3K/AKT/FOXO1 pathway, which causes increased pancreatic and duodenal homeobox 1 (Pdx1) and MafA mRNA expression but causes decreased MAFB expression, may be the mechanism involved in this process.…”
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白细胞介素-17及其受体在垂体瘤中的表达及临床意义

“…【目的】检测人垂体腺瘤组织中白细胞介素(IL-17)及其受体IL-17R的表达，并探讨其在垂体腺瘤侵袭性发病机制中的作用【方法】收集85例人垂体腺瘤组织标本，分成2组：侵袭性垂体腺瘤组45例非侵袭性垂体腺瘤组40例采用免疫组化法检测各标本IL-17IL-17R的表达随机选取其中50例标本(分成2组:侵袭性组25例非侵袭性组25例)，采用RT-PCR法检测IL-17IL-17R的mRNA表达水平分析IL-17IL-17R的表达与垂体腺瘤侵袭性的关系【结果】 免疫组化结果显示:IL-17IL-17R在侵袭性垂体腺瘤中的强阳性及总阳性表达率分别38.0%和83.0%49%和86.7%，而非侵袭性垂体腺瘤中的强阳性及总阳性表达率分别20.0%和52.5%20.0%和62.5%IL-17IL-17R在侵袭性垂体腺瘤中的表达率明显高于在非侵袭性垂体腺瘤中的表达率，统计学分析显示有统计学意义(P < 0.05)RT-PCR结果显示：侵袭性垂体腺瘤中的IL-17IL-17R mRNA的表达水平明显高于非侵袭性垂体腺瘤，差异有统计学意义(P < 0.05)【结论】 IL-17IL-17R在侵袭性垂体腺瘤组织中的表达明显升高，可能促进了垂体腺瘤侵袭性的发生…”
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Effects of Functional Red Pine Seed Direct-Drinking Oil on Constipation and Intestinal Barrier Function in Mice by Jie Li, Haonan Zheng, Jiahui Liu, Jie Ding, Qingqi Guo, Na Zhang

“…Additionally, the protein expression levels of occludin and claudin-1 were reduced by 86.11% and 25.00%, respectively (<i>p</i> < 0.05), while mRNA expression levels decreased by 70.80% and 59.00% (<i>p</i> < 0.05). …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…The METTL3-dependent regulation of DLG2 mRNA stability could be a critical factor in cervical cancer progression.…”
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Tangduqing Granules Attenuate Insulin Resistance and Abnormal Lipid Metabolism through the Coordinated Regulation of PPARγ and DGAT2 in Type 2 Diabetic Rats by Qinghua Zhang, Yingying Huang, Xiaojin Li, Hongyi Liu, Baichuan He, Bin Wang, Yuntao Ma, Xiang Zhou, Yaqin Liu, Shentao Wu

“…Furthermore, TDQ reversed diabetes-induced decrease in the mRNA and protein expression of PPARγ and elevation in the mRNA and protein levels of DGAT2 in the liver. …”
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Analysis of the lncRNA-Associated Competing Endogenous RNA (ceRNA) Network for Tendinopathy by Bing-Zhe Huang, Yang Jing-Jing, Xiao-Ming Dong, null Zhuan Zhong, null Xiao-Ning Liu

“…Moreover, a ceRNA regulatory network was constructed based on the identified lncRNA–mRNA coexpression relationship pairs and miRNA–mRNA regulation pairs. …”
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shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells by Qun Cheng, Shu Zhen Jiang, Li Bo Huang, Wei Ren Yang

“…Compared with the Sh-Nrf2 group, the activities of total superoxide dismutase and glutathione peroxidase and relative expressions of Keap1 at mRNA and protein level in the Sh-Nrf2+ZEA20 and Sh-Nrf2+ZEA40 groups were significantly reduced, the malondialdehyde level, and the fluorescence intensity around and within the nucleus of reactive oxygen species and Nrf2, and the relative expressions of Nrf2, quinone oxidoreductase 1, and hemeoxygenase 1 at mRNA and protein level significantly increased. …”
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Assessing molecular changes underlying isopropylated phenyl phosphate (IPP)-induced larval sensorimotor response deficits in zebrafish by Sunil Sharma, Alfredo Rojas, Abhishek Gour, Rosemaria Serradimigni, Connor Leong, Abhisheak Sharma, Subham Dasgupta

“…We dissected eyes and brains from IPP-exposed larvae and conducted 5-mC assessments and mRNA-sequencing. Interestingly, neither of the organs showed differences in 5-mC levels and the brains also did not show substantial transcriptomic effects. …”
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Activation of Adenosine A3 Receptor Alleviates TNF-α-Induced Inflammation through Inhibition of the NF-κB Signaling Pathway in Human Colonic Epithelial Cells by Tianhua Ren, Yumei Qiu, Weiyun Wu, Xiao Feng, Shicai Ye, Zhuang Wang, Ting Tian, Yanting He, Caiyuan Yu, Yu Zhou

“…Furthermore, 2-Cl-IB-MECA significantly decreased TNF-α-stimulated IL-8 and IL-1β mRNA expression and secretion, compared to the TNF-α-only treated group. …”
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Effects of Different Levels of <i>Lycium barbarum</i> Flavonoids on Growth Performance, Immunity, Intestinal Barrier and Antioxidant Capacity of Meat Ducks by Minhang Tu, Gentan Cai, Longfei Ma, Leyan Yan, Tian Wang, Zhendan Shi, Chao Wang, Zhe Chen

“…Dietary supplementation with 500 mg/kg LBFs significantly up-regulated the mRNA expression of genes associated with intestinal barrier function and antioxidant capacity in the jejunal and ileal mucosa, as well as the protein expression of these antioxidant genes, and led to a significant reduction in the mRNA expression of pro-apoptotic and inflammatory-related genes. …”
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Bmi-1基因沉默对人视网膜母细胞瘤SO-RB50细胞生长的体外抑制作用

“…荧光定量RT-PCR和western-blot分别检测转染Bmi-1 siRNA后的人SO-RB50瘤细胞中Bmi-1 mRNA和蛋白水平的变化。MTT法测定Bmi-1基因干扰后SO-RB50瘤细胞增殖情况。流式细胞仪检测Bmi-1 siRNA对人SO-RB50瘤细胞凋亡的影响。…”
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前列腺素E2体外可促进人CD34+ 细胞增殖 by 赖淑萍

“…real time-PCR检测survivin-mRNA水平?Western blot检测胞浆内β-catenin和survivin蛋白表达情况?…”
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Sustained Interleukin-1β Exposure Modulates Multiple Steps in Glucocorticoid Receptor Signaling, Promoting Split-Resistance to the Transactivation of Prominent Anti-Inflammatory Ge... by Pedro Escoll, Ismael Ranz, Norman Muñoz-Antón, Ana van-den-Rym, Melchor Alvarez-Mon, Carlos Martínez-Alonso, Eva Sanz, Antonio de-la-Hera

“…Compared to GC alone, in a broad range of GC doses plus sustained IL-1β, FKBP51 mRNA expression was reduced by 1/3, TTP by 2/3, and IRF8 was completely knocked down. …”
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Suppressive Effect of Insulin on the Gene Expression and Plasma Concentrations of Mediators of Asthmatic Inflammation by Husam Ghanim, Kelly Green, Sanaa Abuaysheh, Manav Batra, Nitesh D. Kuhadiya, Reema Patel, Antoine Makdissi, Sandeep Dhindsa, Ajay Chaudhuri, Paresh Dandona

“…Following insulin infusion, the mRNA expression of IL-4, ADAM-33, LIGHT, and LTBR mRNA expression fell significantly (P<0.05 for all). …”
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Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway by Xiaomei Du, Kexin Wang, Xiaoyan Sang, Xiangxing Meng, Jiao Xie, Tianxin Wang, Xiaozhi Liu, Qun Huang, Nan Zhang, Hao Wang

“…Moreover, naringin down-regulated the mRNA expressions of inr, chico, pi3k, and akt-1, and up-regulated the mRNA expressions of dilp2, dilp3, dilp5, and foxo, thereby activating autophagy-related genes and increasing the number of lysosomes. …”
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